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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 202 Documents

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Histopathological in Gills, Hepatopancreas and Gut of White Shrimp (Litopenaeus Vannamei) Infected White Feces Disease (WFD) Jefri Anjaini; Mohamad Fadjar; Sri Andayani; Ivana Agustin; Ilham Bayu
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

White Feces Disease which is the presence of white feces floating on the surface of the water. Physical disruption of White Feces Disease attacked by exocytes becoming soft. the cause of white defect disease (WFD) is a decrease in environmental quality such as salinity, DO and ammonia. white feces disease is associated with gregarine protozoa in the Apicomplexa phylum. Gregarine parasitic infection from the type of nematopsis that was interacted with Vibrio and Vibrio type bacteria found in white shrimp (Litopenaeus vannamei) which was attacked by white impurities was V. harveyi. This study aims to analyze the presence of gregarine protozoa in the gills, intestines and hepatopancreas of white shrimp infected with White Feces Disease. The method used in this research is descriptive method. Histopathological results showed that there were protozoa (Gregarine) in the intestine, hepatopancreas and gills of white shrimp infected with WFS in 3 different locations and found Aggregated Transformed Microvilli (ATM) that resembled gregarine.

Identification of Koi Herpesvirus on Koi Fish (Cyprinus carpio) with Immunocytochemistry Test Streptavidin Biotin Rika Wahyuningtyas; Uun Yanuhar
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Koi herpesvirus (KHV) has been identified in common carp (Cyprinus carpio) and varieties of C. carpio such as koi carp. Handling KHV outbreak on koi fish are always late and on case KHV infection, hence the purpose of this study is to find an alternative method in identifying a particular fish diseases KHV which more simple, practical and accurate. 20 samples are positively infected by KHV were derived from Blitar, with the characteristic of them are gill slime, erosion or ulcers on the skin, movement were not controlled, and skin blister. The result of our study showed that 100% positive reaction to the ICC in smear blood, which is marked golden chocolate color. The result of immunostaining streptavidin biotin obtained that koi fish have been infected by severe KHV showed golden chocolate color on the blood smear compared to positive control. While fish have been infected by undemanding KHV infection or still in the beginning stage, the blood smear showed chocolate bluish. It can be concluded that Immunocytochemistry Test Streptavidin Biotin can be used for early identifying of Koi Herpes Virus (KHV) due to simpler producer, more practical, and accurate result.

Investigation of Scutellaria radix on Phenoloxidase and Prophenoloxidase of Giant Freshwater Prawn, Macrobrachium rosenbergii, via Reverse-gavage Feeding Andriawan, Soni; Bao, Hung Tran; Cheng, Phillip T.; Yuniarti, Ating
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Scutellaria radix is one of the ancientest approaches to treat diseases. The aim of this study was to investigate the effect of Scutellaria radix on the immune responses of Macrobrachium rosenbergii by reverse-gavage feeding and their protection against Lactococcus garvieae. In brief, M. rosenbergii was injected with S. radix water extract at 1, 5 and 10 µg/µl via reverse-gavage feeding (RGF). Phenoloxidase (PO) activity of hemocytes and prophenoloxidase (proPO) gene of hepatopancreas were assessed to determine M. rosenbergii immunity. The results revealed that the concentration of S. radix extract at 5 µg/µl enhanced significantly the phenoloxidase of M. rosenbergii correlated with the control group. For pro PO were up-regulated by all concentration in a time-dependent manner. The highest percentage of survival rates was observed at 5 µg/µlof S. radix water extract after challenged with L. garvieae. The present discoveries suggest that S. radix water extract at the concentration of 5 µg/µlmay raise the immune responses and gene expressions in M. rosenbergii using RGF.

Phytogenetic Relationships of Mystacoleucus marginatus (Valenciennes 1842) based on Cytochrome Oxidase C Subunit I (COI) Gene Fitri Sil Valen; Maheno Sri Widodo; Yuni Kilawati; R. Adharyan Islamy
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.3

Mystacoleucus marginatus is a type of freshwater fish that is spread in various countries with tropical climates, one of which is Indonesia. This fish can be found in the Brantas River Basin (DAS), East Java. In its development, identification of fish species needs to be done in detail and more deeply, so that the correct identification results can be obtained. This study aims to identify genetic and phylogenetic characteristics of Mystacoleucus marginatus in the Brantas River Basin based on the Cytochrome Oxidase C Subunit I (COI) gene. Test samples were taken from 11 individuals of Mystacoleucus marginatus in the Brantas watershed, from June to August 2018. The results showed that Mystacoleucus marginatus in the Brantas watershed had 2 Haplotypes with Haplotype (Hd)=0.96 diversity values and nucleotides diversity (Pi)=0.00357. These results indicate that the population in the Brantas watershed is interconnected between one location and another sampling location, this is supported by a phylogenetic topology of DNA barcodes showing a monophyletic clade, as well as a haplotype distribution indicating that the sample area is genetics are not isolated from one another. So that the type of Mystacoleucus marginatus scattered along the Brantas watershed is still in one species, which is indicated by the low level of genetic variation. Genetic identification efforts must be carried out continuously so that appropriate information can be obtained for the diversity of fish species, especially endemic fish.

Minimum Inhibitory Concentration of Marine Microalgae Dunaliella salina on Fish Pathogenic Bacteria Edwardsiella tarda Rusmawanto, Rusmawanto; Prajitno, Arief; Yuniarti, Ating
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Dunaliella salina is a type of marine microalgae. The objective of this research were investigated the effect of D. salina to inhibit the growth of E. tarda. The method used in this study was examined antibacterial activity of D. salina using disc diffusion and co culture test. The secondary metabolite compound in D. salina was tested using phytochemical screening and FTIR methods. The results obtained D. salina extracted using n-hexane showed the best activity for inhibiting the growth of E. tarda. Minimum concentration of 100 mg.L-1 crude extract can inhibit E. tarda with a total of bacterial colonies 137x1011 CFU.mL-1. The phytochemical test results showed that D. salina extract using n-hexane contained flavonoids, saponins, alkaloids, terpenoids and phenols. The FTIR analysis showed phenol and carotene compounds are one of the secondary metabolites that can be used as antibacterial. It can be concluded that the extract has strong antibacterial activity against E. tarda and potentially as antibacterial in aquaculture.

Accumulation of Heavy Metals Lead (Pb) and Copper (Cu) in Mangrove Area of Avicennia marina in Manyar Subdistrict, Gresik District, East Java Made Dwipa Kusuma Maharani; Asus Maizar Suryanto Hertika; Muhammad Musa
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Human and industrial activities in the area of Manyar Subdistrict Mangrove Waters, Gresik District can increase pollution and the presence of heavy metals in aquatic environments and aquatic organisms. This triggers input of heavy metals, especially Pb and Cu. Mangroves can accumulate and have a high tolerance for heavy metals so they can be used as plants for phytoremidiation purposes (phytostabilization). The mangrove ecosystem can be used as a control of heavy metal pollution and can also be used as a pollutant trap. This study was conducted to determine the potential of phytoremidiation based on the accumulation and translocation of heavy metals in Avicennia marina mangroves in absorbing heavy metals Pb and Cu from their environment. Based on research results MacFarlane et al., (2007) mangrove Avicennia marina is one of the plants that can effectively accumulate heavy metals due to its root system. The sample was analyzed using AAS (Atomic Absorption Spectrophotometer). The research parameters include levels of Pb and Cu metals in sediments, mangrove roots and leaves. The results of Pb heavy metal concentrations ranged from 0.30 ppm - 4.84 ppm and Cu ranged from 0.27 ppm - 11.42 ppm with the highest values found in sediment stations 2. Avicennia marina at the study site was excluder because the BCF value < 1 which ranged from 0.02 - 0.18 ppm and phyto-extraction because the value of TF > 1 ranged from 0.35 to 1.69 ppm. The results of this study indicate that Avicennia marina mangroves can be developed into phytoremidiation agents because they are able to absorb and transfer heavy metals from the environment to other body tissues.

Body Dimension Factors Affecting Live Weight Estimation Accuracy of Indonesian Local-Thoroughbred Racing Horse Santie Turangan; Umar Paputungan; Surtijono Edmundus Siswosubroto
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.5

Study was conducted to estimate live weight of Indonesian Local-Thoroughbred racing horse using their chest girth, body length and body volume represented by chest girth and body length dimensions. Data on animal live weight (LW), body length (BL), chest girth (CG) and body volume were collected from male horses (n=188) kept by the owner club members of North Sulawesi Racing Horse. Animal body volume was calculated using cylinder volume formula with CG and BL as component factors of the formula. Data were classified on animal age basis of five groups. Regression analysis was applied for LW with all linear body measurements. Age significantly influenced all body measurements. Simple regression can be recommended to predict live weight of this animal based on body volume with their average age groups as follows: Live weight (Y in kg unit) = - 0.002+1.16 body volume (dm3) with determination coefficient (R2) of 0.98. The multiple regression can also be recommended to estimate accurately live weight of this animal at age average groups as follows: Live weight (Y in kg unit) = - 0.11 - 2.74 chest girth (X1 in cm unit) + 4.92 body length (X2 in cm unit) with R2 of 0.98.

Effect of Sweet Purple Potato Extract (Ipomoea batatas L) on Zebrafish (Danio rerio) by Diet Induced Obesity Aswaty Nur; Yhusi Karina Riskawati; Edwin Widodo; Retty Ratnawati; Amirah Firdausy; Lydia Kusuma Dewi; Maria Karina
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The effect of a high-fat diet on zebrafish as an alternative experimental animal in studying lipid metabolism is not fully understood. In this research, sweet purple potato extract (SPPE) which has anthocyanin have used as an anti-obesity property. Zebrafish aged 8 mpf was divided into five groups: Non-DIO (control negative), DIO (Diet-Induced Obesity, control positive), and three experimental groups (DIO A, DIO B, DIO C). Each group consisted of five Zebrafish. All groups have been fed freshly hatched Artemia for 40 days where the Non-DIO’s amount was 5 mg Cyst/fish one time per day, and the other groups’ amount was 60 mg Cyst/fish three times per day. The experimental groups were exposed to sweet purple potato extract three times per day by different SPPE doses: DIO A by 80 ppm, DIO B by 120 ppm, and DIO C by 160 ppm. The results show that there was no significant difference between the positive control group and the experimental groups in term of feeding intake, BMI, blood glucose, and total cholesterol. However, based on molecular parameters, there was a declining trend on PPAR-γ level, although, the adiponectin level remained stable. We suggest that anthocyanin does not affect feed intake, BMI, blood glucose level, cholesterol, and expression of PPAR-γ and adiponectin of zebrafish with diet-induced obesity.

Isolation, and Identification of Diesel Oil Degrading Bacteria in Water Contamination Site and Preliminary analysis with Potential Bacterial Gordonia terrae Ainun Ramadhani Tri Wahyuni; Endang Yuli Herawati; Andi Kurniawan; Abd. Aziz Amin
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Bioremediation is one of kind method which use of bacteria to degrade of hydrocarbon to be another compound. This study aims to get isolation of indigenous bacteria and potential test with Gordonia terrae on diesel oil bioremediation. The water sample was taken from Tanjung Perak Port of Surabaya, Indonesia where contaminated with diesel oil. Analysis of in-situ water quality parameters were consist of Dissolved oxygen, temperature, pH, salinity. Strain isolate bacteria were isolated by Bushnell-Hass medium. The bacterial density result shows in IN6 shows that bacterial density increased up to 9 × 108 cells/ml at day 2 incubation and decrease up to 1.79 × 108 cells/ml at final incubation, IN8 increased up to 8.6 × 108 cells/ml, then decrease up to 3.57 × 108 cells/ml. While, EX8 increased up to 9.1 × 108 cells/ml, and then decrease up to 3.06 × 108 cells/ml. While, results of degradation diesel oil hydrocarbon show treatment IN6, TPH values showed a decrease in the initial hydrocarbon concentration of 30 ppm to 16 ppm. IN8 TPH value shows a decrease in the initial hydrocarbon concentration of 30 ppm to 16 ppm. EX8 TPH value shows a decrease in the initial hydrocarbon concentration of 30 ppm to 24 ppm. In this experiment was found that Bacillus cereus was effectively degrading of diesel oil and can be agent bacterial for bioremediation.

The Effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) In Type 2 Diabetes Mellitus Rizky Amalia; Karyono Mintaroem; Loeki Enggar Fitri; Nashi Widodo; Hidayat Sujuti
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.6

Diabetes is a disorder in the metabolism of carbohydrates, lipids and proteins with various causes. From the previous research, GPR43 is a potent clinical target for the treatment of type 2 diabetes. It can be activated by Short Chain Fatty Acid (SCFA) which is an organic fatty acid produced through fermentation of dietary fiber by bacteria in the distal intestine. The aim of this study was to analyze the effect of C680T GPR43 Gene Variations to Its Interaction with Short Chain Fatty Acid (SCFA) in silico. The study used 8 sequences of GPR43: ID AF024690, AC002511, EU432114, BC095535, BC096198, BC096199, BC096200, BC096201 and data of ligand (SCFA): acetic acid (CID 176), Butyric acid (CID 264) and Propionic acid (CID 1032). GPR43 was modelled using I-TASSER, Sequence and structural alignments were conducted using Bioedit and Superpose V.10, respectively. The Docking process was done using PyRx and molecular interaction was analyzed using Discovery Studio 2016. The Result showed that three types of SCFA bind to GPR43 variants T and GPR43 variant M obtained a similar pattern. The binding affinity from the largest to the smallest is butyric acid > propionic acid > acetic acid. GPR43 variant T has greater affinity to the SCFA than the GPR43 variant M. There is no differences of preference between GPR43 variant T and GPR43 variant M to bind SCFA.

Page 11 of 21 | Total Record : 202

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

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Contact Name
Kuswanto

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rjls@ub.ac.id

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+62341 575825

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rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA