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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 202 Documents

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Use of Six Planting Media on Shallow Bed and Plant Container Systems to The Growth and Yield of Sweet Potato (Ipomoea batatas L.) for Roof Garden Sitawati Sitawati; Agus Suryanto; Euis Elih Nurlaelih
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Roof garden is an alternative to overcome food security through breeding crops and horticultures of fruits and vegetables. The research was conducted in Malang by applying two planting systems, such as shallow bed (direct cropping) and plant container bag by the same planting medium treatments. Research, which applied shallow bed and container bag, was conducted using Randomized Block Design (RBD) by 6 treatments of planting medium and 4 replications, such as : Soil (100%) (M0), Soil + Charcoal (1:1) (M1), Soil + Cocopeat (1:1) (M2), Soil + Compost (1:1) (M3), Soil + Manure (1:1) (M4), and Soil + Moss (1:1) (M5). Data analysis of the research used analysis of variance, and if any significant influence was found, it would be followed by the Least Significant Difference (LSD) at level 5%. And then, it would be followed by T-test as comparison between shallow bed system and container bag system. Parameters that being observed in the research included length of crop, leaf area, numbers of tuber, fresh weight of tuber, dry weight of tuber, and total dry weight of plants. Results of the research showed that the application of medium types of soil and manure (1:1) by shallow bed system on Roof Garden concept may increase numbers of tuber, fresh weight of tuber, dry weight of tuber, and dry weight of Sweet Potato (Ipomoea batatas L.) in comparison with the application of soil medium and manure (1:1) by plant container bag system.

The Effects of Non-Genetic Factors on The Birth Weight, Litter Size and Pre-Weaning Survive Ability of Etawah Cross-Breed Goats in The Breeding Village Center in Ampelgading District Tri Eko Susilorini; Kuswati Kuswati; Sucik Maylinda
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

A total of 106 late gestation goats (age 4-5 months of pregnancy) records each of Etawah Crosbred goats kept on small farmer over a period of 10 years were assessed to determine the effects of environmental factors on them. Body Condition Score is non-genetic factor and in dairy goats to predicted milk production, fertility, and general health of the animal because BCS is visualisasi from feeding manajement. The objective of this study was to investigated the relationship between Body Condition Score (BCS) of late gestation with litter size and birth weight on etawah crossbred goats. The results showed that the average of litter size and birth weight (kg) were 1.89±0.66 and 3.84±0.73 respectively. The relationship between BCS with litter size were 0.13 (very low) and the relationship between BCS with birth weight were 0.11 (very low). The conclusion of this research is BCS of late gestation had positive correlation on litter size and birth weight, however this was low and birth weight as first indicator of future growth rate.

The Linnaeus’s Reed Snake, Calamaria linnaei Boie (Squamata: Colubridae: Calamariinae) from Ijen Plateau, East Java, Indonesia Irvan Sidik; Sutiman Bambang Sumitro; Nia Kurniawan
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The decisive morphological character of Calamaria linnaei from the Ijen plateau was analyzed in an attempt to evaluate these snakes independently achieved as different populations or whether more likely only cryptic species affected by natural conditions. The evidence was concluded that a cryptic species is very interesting to be regarded as something entirely different. In general, however, the population appears to have more closely related to other congeners populations of the same species. It is suggested that at least temporarily, the C. linnaei population of the Ijen plateau be specifically considered to include in species with high individual variations based on limited geographic boundaries until molecular analysis proves it.

Potential of Topical Curcumin in Reduction of TNF-α expression and Synovium Hyperplasia on Wistar Rats of Rheumatoid Arthritis Model Ferri Widodo; Diana Lyrawati; Bagus Putu Putra Suryana
Research Journal of Life Science Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Rheumatoid arthritis is a chronic inflammatory autoimmune disease associated with articular and systemic effects. This disease affects synovial joints covered by a special tissue called synovium. Curcumin has a potent antioxidant, antiinflammatory agent, antiangiogenic and anticarcinogenic. Curcumin can downregulate the expression of various proinflammatory cytokines and is reported beneficial effects in arthritis, but has a poor solubility dan bioavailability as well. The purpose of this research was to study the potential of liposomes topikal curcumin in reducing athritis score, reducing the expression of TNF-α and histopathological synovium hyperplasia of hind paw on Wistar rats with CFA that had been treated with topical curcumin. In this study, rats were divided into 7 groups: positive control, negative control, rheumatoid arthritis with topical curcumin therapy of 90 mg/kg BW, rheumatoid arthritis with topical curcumin therapy of 110 mg/kg BW, rheumatoid arthritis with topical curcumin therapy of 200 mg/kg BW, rheumatoid arthritis with methotrexate therapy, rheumatoid arthritis with placebo therapy. Results from this experiment indicated that topical curcumin has no significant to the arthritis score, significantly effect to percentase expression of TNF-α (p<0.05) and could decrease synovium hyperplasia based on histophatology examination. It could be concluded that therapy of topical curcumin could decrease the expression of TNF- α and synovium hyperplasia in rheumatoid arthritis rat.

Management Restoration Plans for Coastal Villages Rudianto Rudianto; Edi Susilo; Supriyatna Supriyatna
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The village is located in the coastal area up to this time has decreased the quality of the coastal environment either caused by the process of natural or anthropogenic processes. Coastal damage Persistent will affect people's lives. Based on studies conducted by Rudianto (2013) and continued research by Rudianto (2014) on the institutional model for implementing the strategy, the resulting output coast of research called restoration plan for coastal villages or R2DP coastal villages.The objective of R2DP is helping the village administration to alleviate the problems of coastal areas. R2DP is a guideline that will be used by the village government based on a legal framework called the village regulations.The method used to compile R2DP is descriptive method. By using the method of Miles and Huberman (1984) used data reduction techniques. This technique is to pick and choose which data is critical to focus on the purpose of research.the results of research to produce findings about the restoration plan or a coastal village called R2DP. The essence of the mechanism and procedure R2DP is doing the restoration work by using institutions as a means of restoration.

Stock Identification Sardinella Lemuru, Bleeker 1853 In Madura Strait, Bali Strait, and Southern-East Java Water Setyohadi, Daduk; Iranawati, Feni; Hikmah, Syarifah
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Balinese Sardinella (Sardinella lemuru) is the main commodity in Indonesia. Despite, more research show that lemuru got the overfishing condition. In East Java region, likely Bali Strait, Southern-East Java water, and Madura Strait are the region which the most production of lemuru catch. So it needs identification stock of Sardinella lemuru on the third fisheries management east java sub-region, as it to be used as a reference to managing the sustainability of the catch sardinella lemuru for the future. Research carried out in January to May 2016 with the methods is the sample of random sampling on three different locations water representing Madura Strait, Bali Strait, and southern-East Java water. The each sample implemented were in four times during a period of months old different. Every the sample researchers get as 50-200 tail. Sardinella lemuru in accordance with the season fish. The sample which has been obtained taken to the laboratory of Marine Science University of Brawijaya to data take. The analysis used in this research was Truss morphometric and Factor analysis of the condition allometric. Based on the results of the analysis in the Madura Strait, Bali Strait, and Southern-East Java water are having a distinction of morphometric character amount 44 % with the character being very affected is PAL_FL, DFB_HL , PVL_FL, UPCL_FL, OD_HL, and PPL_FL. Around 64 % of the whole morphometric character that is 14 characters. Next, if it is seen from Allometric condition in the third these waters having b value different from each aquatic and after B-test that lemuru in the three the waters might otherwise dissimilar stock so management of Sardinella lemuru in the three waters should have been undertaken in separated.

Saxitoxin Level Comparation in Bali Sardine (Sardinella Lemuru) in Bali Strait in Different Monsoons Endang Yuli Herawati; Mohammad Mahmudi; Fani Fariedah; Ruly Isfatul Khasanah
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Sardinella fish (Sardinella lemuru) is a species of important pelagic fish from the family of Clupeidae found in Bali Strait. The dynamic condition of Bali Strait makes it fertile which is identical with plankton blooming. The objective of this research was to understand the potential of saxitoxin in sardinella fish because of dinoflagellates consumption in Bali Strait. The research was conducted in Southeast Monsoon (June - August 2015) and Northwest Monsoon (December 2015 – February 2016). The method used was in situ plankton sampling, counting and ELISA test to determine the level of saxitoxin in the fish. Hydro-oceanographic parameters measured were temperatures, salinity, DO (dissolved oxygen), pH level, phosphate level, nitrate level and transparency. They showed average optimum value for phytoplankton growth especially during Southeast Monsoon which its temperature lower and the nutrients (phosphate nitrate) higher than the Northwest Monsoon. This condition caused high richness of phytoplankton in the water and followed with dinoflagellates richness so they accumulated in sardinella fish. It was supported by the analysis of the fish gastric which showed positive correlation between dinoflagellates richness in the water and the fish gastric. ELISA test also showed that saxitoxin level of fish caught in Southeast Monsoon was higher than the one caught in Northwest Monsoon even though the level of saxitoxin was still in the safe range (less than 80 μg STXeq. per 100 g) but the danger of saxitoxin accumulation should be watched out for.

Enzymatic Cellulose Palmitate Synthesis Using Immobilized Lipase Anna Roosdiana; Diah Mardiana; Ellya Indahyanti; Dyah Ayu Oktavianie
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Bacterial cellulose can be modified by esterification using palmitic acid and Mucor miehei lipase as catalyst. The purpose of this research was to determine the optimum conditions of esterification reaction of cellulose and palmitic acid . The esterification reaction was carried out at the time variation of 6, 12, 18, 24 and 30 hours and the mass ratio of cellulose: palmitic acid (1: 11: 2, 1: 3, 1: 4, 1: 5,1:6) at 50 °C. The cellulose palmitate was examined its physical and chemical properties by using FTIR spectrophotometer, XRD, bubble point test and saponification apparatus. The results showed that the optimum reaction time of esterification reaction of cellulose and palmitic acid occurred within 24 hours and the mass ratio of cellulose: palmitic acid was 1: 3 resulting in DS of 0.376 with swelling index of 187 %, crystallinity index of 61.95%, and Φ porous of 2.40 μm. Identification of functional groups using FTIR spectrophotometer showed that C=O ester group was observed at 1737.74 cm-1 and strengthened by the appearance of C-O ester peak at 1280 cm-1. The conclusion of this study is reaction time and reactant ratio influence significantly the DS of cellulose ester.

Development of Candidate Antigens for Rapid Test Kit to Detect Autoantibodies in Patients with Systemic Lupus Erythematosus Wisnu Barlianto; Hani Susianti; Singgih Wahono; Nelly Ismayasih; Rossy Meilani; Kusworini Handono
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Systemic Lupus Erythematosus (LES) is an autoimmune inflammatory disease characterized by the formation of anti-nuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) antibodies as diagnostic markers. Detection of such autoantibodies requires advanced equipment and trained personnel.This study was conducted to acquire candidate antigens that can be used for rapid test kit for practical and accurate detection of ANA and anti-dsDNA to speed up SLE diagnosis.Nuclear proteins and DNA derived from cell lines, hair follicles, and leukocytes of SLE patients and healthy individuals were isolated using QiaGEN kit and modified-manual procedure. Antigen-antibody bonds were tested by dot blot assay.The strongest binding between DNA antigens of a healthy individual and antibodies occurred at dilution factors of 1:5,120 for the antigen and 1:2,560 for the antibody. The strongest binding between nuclear protein antigens from the cell line and antibodies occurred at dilution factors of 1:512 for the antigen and 1:1,600 for the antibody.Nuclear antigens derived from cell line and DNA antigens of healthy individuals were antigen candidates for the development of ANA and anti-dsDNA rapid detection tests.

The Comparison of Chromosome Analysis Result by Manual and Software Cytovision Image Analysis Using Simple G-Banding Gatot Ciptadi; Muhammad Nur Ihsan; Sri Rahayu; Veronica Margareta Ani Nurgiartiningsih; Mudawamah Mudawamah; Ardyah Ramadhina Irsanti Putri
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Chromosome analysis or karyotyping is one among powerful methods to characterize normal or abnormal genetic of animals. On the basis of the important chromosome abnormalities and their negative effect in the near future, chromosomal investigation of breeding bull especially for Artificial Insemination (AI) began in different countries. Chromosomal abnormalities are usually considered to be a plague and are to eliminate. In Indonesia, AI implementation in cattle have been started intensively, especially using imported bulls (bos Taurus) i.e. Limousine. A number of cattle breeds have been reported on of the 50 exotic breeds with the problem of 1/29 translocation, then the chromosome analysis to be important to execute. Method performed by collecting blood samples from first generation of crossing breed Madura Cattle vs. Bos Taurus. Sample was added to medium (Karyo MAX Gibco) then placed in CO2 incubator at 38°C. Colcichine was added after 70 hours and kept for 2–3 hours. Slides were prepared and dried then stained with Giemsa. Slides were examined under high phase-contrast microscope, chromosome analysis using cytovision software and manual analysis straightly captured under microscope then arranged. Result of both method of karyotyping may accepted for analysing method of abnormal/normal chromosome. It showed that the 2 N diploid number of chromosome was normal was 60, there were 58 autosomes and 2 sex chromosomes in all cattle observed. It was observed that all cattle tested in this research were normal categories. The karyotype analysis of all cattle showed that the chromosomes of one cell and different individual each breed varied in size, shape and position of centromere. It was recommended to performed chromosomal investigation of breeding bulls using advanced sophisticated tools of analysis like cytovision image analysis of fluorescent technique. Manual method was recommended only for analysis of normal or abnormal number of chromosome.

Page 8 of 21 | Total Record : 202

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Kuswanto

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rjls@ub.ac.id

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+62341 575825

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rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA