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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 202 Documents

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Determination Amylolitic Characteristic of Predominant Lactic Acid Bacteria Isolated during Growol Fermentation, in a Different Starch Medium Composition Putri, Widya Dwi Rukmi; Zubaidah, Elok; Masahid, Ardiyan Dwi
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

In order to achieve efficient lactic acid production from starch, fermentation of avarious composition starch medium by lactic acid bacteriawas examined in this study. Many strains of Lactobacillus plantarum isolated from growol fermentation, Lactobacillus plantarumsubsp. plantarum NBRC 15891 and Lactobacillus amylophyllus NBRC 15881 were used as starter cultures in starch basis medium, i.e, basal, basal-starch, enriched basal-starch with polypeptone and yeast extract. Lactobacillus plantarum UA3, AA2, AA11 showed the highest cells growth compare to both reference strains, but Lactobacillus amylophyllus NBRC 15881 showed a greater ability to degrade starch indicated by decreasing of pH and starch content of the fermented substrate. Enriched medium with peptone and yeast extract could generate the growth and starch degradation capabilities for all types of lactic acid bacteria were used.

Knowledge and Socioeconomic Status as The Factors of Pre-hospital Delay in Patients with Acute Coronary Syndrome Yustina Ni Putu Yusniawati; Yuyun Yueniwati; Dewi Kartikawatiningsih
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Acute Coronary Syndrome (ACS) is a cardiac emergency condition that requires fast and precise action while a pre-hospital delay in a patient to come to the ED is still common. This pre-hospital delay is related to the socioeconomic and knowledge factor. The purpose of this study is to determine the relationship of socioeconomic and knowledge with the pre-hospital delay of ACS patients to come to Integrated Heart Service in Emergency Department of Sanglah General Hospital, Denpasar. This research is a quantitative research with cross-sectional design and purposive sampling as data collection technique with 292 patients. The results showed that 55.8% of patients have low socioeconomic status and there are 71.6% of patients who do not know ACS. The pre-hospital delay, in this case, is associated with low socioeconomic status and knowledge of ACS so that health workers need to provide counseling to patients and families about ACS signs and symptoms, healthy lifestyles, and the importance of coming to theemergency department when signs of ACS are felt.

Size Distribution of Massive Porites at Reef Flat in Kondang Merak, Malang, Indonesia Oktiyas Muzaky Luthfi; Putri Zulaikhah Alviana; Guntur Guntur; Sunardi Sunardi; Alfan Jauhari
Research Journal of Life Science Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Kondang Merak was lied on south of Java sea that has monitored coral reef inside. Environment condition such as load of terrestrial’s nutrient into the sea, increase of surface water temperature and tourism activities were affected on coral reef health. The massive Porites that common coral found in this location need be monitored gradually to see coral condition over time. The research was conducted on May 2015 in reef flat area of Kondang Merak. The aims of the research were to know distribution of massive Porites and oceanography condition in Kondang Merak. The highest sea surface temperature was 26,46o C in transect number 2 at station 1 (eastern part). Salinity of sea water was in normal range between 34, 42o/oo to 35,75o/oo, dissolved oxygen was on range 6 mg/l sampai 9,1 mg/l and pH was 7,23 sampai 8,68 respectively. Two species on massive Porites they were Porites lobata dan P. lutea. In station 1 the average diameter of P. lobata was 228.57 cm and P. lutea 63.89 cm, in while P. lobata in station 2 was 260.57 cm and P. lutea 153.56 cm.

Analysis of Food Composition of Yellowstripe Trevally (Selaroidesleptolepis) In Paciran Waters, Lamongan, East Java Dwi Candra Pratiwi; Defri Yona; Mulyanto Mulyanto; Yusrina Rizqi Amalia
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Paciran is one of the regions in East Java with great fisheries potential. Yellow Stripe Trevally is well known for its abundance in waters and categorized as high economic value within fisheries commodity. The abundance of yellow stripe trevally highly depends on food availability within its living habitat. The aim of this study was to analyze yellow stripe trevally food composition and its relationship with plankton abundance. The result of this study shows that phytoplankton abundance (21,600x103cell/m3) was found higher than zooplankton abundance (9.093x103ind/m3). The highest abundance of phytoplankton was found in Bacillariophycae (21,552x103 cell/m3) and the lowest was found in Coscinodiscophyceae (48.197x103 cell/m3). Between two classes of zooplankton found in this study, the abundance of Maxillopoda class (8.184x103ind/m3) was found higher than Oligotriceae class (0.909x103ind/m3). The composition of plankton in the stomach of yellow stripe trevally have similar pattern with the compositions of plankton in the waters.Based on the calculation of electivity index, there were seven genus that have a value 0<Ei<1 and it was considered as food options of yellow stripe trevally. The selected food compositions were devided into six genus of phytoplankton, those were Asterolampra sp, Coscinodiscus sp, Cyclotella sp, Dinophysis sp, Oscillatoria. sp, Pleurosigma sp, Prorocentrum sp, and a zooplankton genus, Calanus sp. The result of main component analysis shows that water clarity parameter had significant impact on the water quality, while pH was correlated with phytoplankton abundance within in the Paciranwaters.

Identification of Plankton on Fish Pond of Oreochromis niloticus Infected by Viral Nervous Necrosis Uun Yanuhar; Muhammad Musa; Dyah Tri Rahayu; Diana Arfiati
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The purpose of study is to determine the type of plankton found in cultivation pond of nile tilapia infected by VNN. The method is to identify the plankton in the water pond and testing the VNN that infects nile tilapia related to plankton eaten using Polymerase Chain Reaction (PCR). VNN identification results on the nile tilapia digestive tract stomach and intestines as well as plankton found in the stomach and intestines indicate VNN positively. Plankton found in the digestive tract of fish is division of Chlorophyta, Bacillariophyta, phylum Rotifera and Crustacea. It indicates that nile tilapia infected by VNN both on the gastrointestinal tract organs, stomatch or intestines and plankton were found with positive VNN digestive duct, while plankton found in water pond is VNN negative. The VNN infection depends on the nature of specifications VNN on nile tilapia and the plankton in the nile tilapia digestive duct.

Prebiotics Activity of Laminaran Derived From Sargassum crassifolium Anies Chamidah; Hardoko Hardoko; Asep Awaludin Prihanto
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The objectives of this study were to evaluate the prebiotic activity based on the change in cell biomass of the probiotic strain after 24-h growth in the presence of Laminaran from Sargassum crassifolium with the methods of laminaran acid extract (LAE) and laminaran modified extract (LME), inulin, or glucose-relative against the change in cell biomass of Escherichia coli FNCC 0091 grown under the same condition. Prebiotic activity was calculated for L. plantarum FNCC 0051 and Bifidobacterium longum FNCC 1081. The results showed that the increasing cell number of L. plantarum was higher in both substrates LAE and LME (0,58 and 2,03log cycle), whereas that of B. longum was lower. The higher prebiotic activity score obtained for L. plantarum and B. longum grown on LME were positive (0,26 and 0,96 log cycle), whereas the lowest score was for L. plantarum and B. longum grown on LAE, which were negative (-0,35 and -0,31 log cycle), but the higher prebiotic activity score was obtained for inulin (4,08 and 4,78 log cycle). It could be concluded that Laminaran Modified Extract (LME) has potential as prebiotic source, but its potential was lower than inulin.

Application of Optimal Control Strategies for the Spread of HIV in a Population Marsudi Marsudi; Noor Hidayat; Ratno Bagus Edy Wibowo
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This paper presents an application of optimal control theory to assess the effectiveness of control measures on the spread of HIV in a population. This paper formulates and analyzes a deterministic mathematical model with use of condom, screening and therapy as control variables using optimal control theory and Pontryagin’s Maximum Principle. It formulates the appropriate optimal control problem and investigate the necessary conditions for the disease control in order to determine the role of unaware infectives in the spread of HIV using of condom, screening of unaware infective and antiretroviral therapy are used as the control items. The optimality system is derived and solved numerically.

Urine Specific Proteins and Alpha-1 Antitrypsin Concentrations to Assess the Severity of Lupus Nephritis Hani Susianti; Wisnu Barlianto; Dian Sukma Hanggara; Kusworini Handono; Purwanto Adipireno; Lisyani Suromo
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Background. Current biomarkers for evaluating disease activity or severity in lupus nephritis (LN) are considered to be unsatisfactory. Pathological changes in glomerular basement membrane and selectivity of electrical discharge are causing specific patterns of urine proteins excretion. Together with alpha-1 antitrypsin (AAT), they are expected to become new biomarkers to assess LN activity.Method. Seventy-one urine samples were collected from healthy controls and LN patients. Patterns of urine specific proteins were determined using column chromatography and SDS-PAGE tests, LN activity was calculated using SLEDAI-renal domain score, and AAT concentrations was measured by ELISA.Result. The majority of proteins in the control group have molecular weights of >66 kDa (88%) and 21- to 25-kDa proteins were observed only in the case group. The p values for differences in urine AAT concentration between active LN and healthy controls, inactive LN and healthy controls, and active LN and inactive LN were 0.004, 0.046, and 0.054, respectively, whereas those for urine AAT/creatinine ratio were 0.489, 0.019, and 0.915, respectively.Conclusion: There were differences in the patterns of the molecular weight of proteins and urine AAT concentrations between case group and control group. However, no such differences were identified between active and inactive LN.

The Effect of Used Form and Level Green Cincau Leaves (Cycleabarbata L. Miers) as Feed Additive on Broiler Performance Production Muhammad Halim Natsir; Osfar Sjofjan; Muharlien Muharlien
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aimed research was to examine the effect of used form and level green cincau leaves (Cycleabarbata L. Miers) as feed additive on broiler performance (feed consumption, body weight gain, feed conversion ratio and income over feed cost). The materials used were 192 Day Old Chicks (DOC) of Lohman MB Platinum with average initial body weight of 37.4±2.87 g, encapsulated or non encapsulated green cincau leaves (Cycleabarbata L. Miers) and encapsulants. Encapsulants that used was the mixture of gum arab-whey (4:1) wich was added BHT 0.06%. Encapsulants that used was 25% of green cincau leaves (Cycleabarbata L. Miers). The method in this experiment was in vivo experiment with Nested Completely Randomized Design with 2 form of green cincau leaves (Cycleabarbata L. Miers) (non encapsulated and encapsulated) and 4 levels of inclusions 0, 0.5, 1.0 and 1.5%, if there were significant effect it would be future tested with Duncan’s Multiple Range Test. The result showed that green cincau leaves (Cycleabarbata L. Miers) in either encapsulated and non encapsulated form didn’t significantly effect (P>0.05) on consumption, feed conversion ratio and IOFC, but it significantly affected (P<0.05) body weight gain and they tended to increase (P<0.01) production index. Increasing levels of green cincau leaves (Cycleabarbata L. Miers) form 0 to 1.5% in encapsulated and non encapsulated form didn’t significantly improve feed conversion ratio, IOFC and production index, thought they tended to increase (P<0.05) feed consumption and they tended to increase (P<0.01) body weight gain. The conclusion of this research was encapsulated better than non encapsulated green cincau leaves (Cycleabarbata L. Miers). The optimal level of feed additive in encapsulated green cincau leaves (Cycleabarbata L. Miers) was 1.0%.

The Analysis of Pandanus Relationship of Purwodadi Botanical Garden Collections Based on Morphological Character and Molecular Marker (trnL and trnL-F) Rachma, Risca Adiyani; Hendrian, Hendrian; Azrianingsih, Rodiyati
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The morphological characteristics of plant vegetative organ has the potential to be the defining factor of pandanus family collection at Purwodadi Botanical Garden (PBG). However, the information is not enough to confirm the identity of pandanus at the type-level. This problem happened at the collection of PBG. It is an urgent issue considering PBG as one of the most important ex-situ plant conservation institute that also has function as a study source for plant taxonomy science in Indonesia. Therefore, the use of molecular technology analysis using molecular marker (primer) is incredibly important to identify the member of pandanus family by identifying its family relationship pattern. This information was expected to give a clarity about the identity of Pandanus family at PBG which will support further research and activity for pandanus conservation. The plant sample includes 16 specimens of pandanus with one member of the family from Freycinetia as the outgroup. Morphological analysis of the 50 characteristics of vegetative organs (leaf, stem, and root) was conducted. Molecular analysis of DNA using primer of trnL and trnL-F. The data sequence as a result of the analysis was then tested using homology test with BLAST. All of the DNA sequence was paralleled using Clustal W analysis, the tree construction relationship was analysed using Maximum Parsimony (MP) method with MEGA 6.0 program. The results of morphological studies showed the character vegetative that is the position of the spines on the leaves, the shape of apex leaf and the condition of the root surface above the soil. Molecularly, appears 1 new genera that has not been previously identified by PBG researcher is Benstonea.

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Kuswanto

Contact Email
rjls@ub.ac.id

Phone
+62341 575825

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rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA