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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 202 Documents

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Post Thawing Sperm Quality and Ca+2 Intensity Characters of Local Goat Sperm After Freezing by Simple Method Using Deep Freezing Gatot Ciptadi; Muhammad Nur Ihsan; Sri Rahayu; Sri Wahjuningsih; Anis Mei Munazaroh; R. P. Putra
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The objective of this research was to determine the effect of the simple modified freezing method, 1°C/minute freezing rate with different diluter ration on a post-thawing quality of local goat sperm namely Peranakan Etawah (PE). This work is aimed to study the quality of post-thawing sperm and to characterize the calcium intensity profile of both fresh and post thawing goat sperm. The method used is the experimental design of a laboratory. Freezing semen was performed in 2 main temperatures of -45°C then -196°C respectively using Mr. Frosty (®) System. Early Sperm characters of Ca+2 intensity was performed by Confocal Laser Scanning Microscope (CLSM) through Fluo-3 staining and Ca++ intensity was analysis descriptively. The result showed that post-thawing qualities are considered as good as standard qualities, at least, more than 40% based on Indonesian National Standard (SNI, 2014). The different level diluents commercial of Andromeda used were influenced highly significant (P<0.01). The best diluents ration is 1:4 (v/v) for final sperms stocked at -196°C. However freezing sperm conserved in -196°C is better than in -45°C. Meanwhile, the sperm characters of two condition showed the important variation of Ca+2 intensity, with the length of region measurement of 39.06±4.595 and 32.696±9.011 µm each. It was concluded that the calcium intensity pattern was varied more and higher in fresh sperm than in freezing sperms. This simple modified method of a freezing system was considered as a feasible alternative method for goat semen in a reason for both for sperm post-thawing quality and practical purposes.

Ethanol Fermentation Potency of Wild Yeast Which Isolated From Soil Drive Nutrient (Sdn) Plantation System Anton Muhibuddin; Indira Rizqita Ivanesthi; Sri Nurhatika; Tjuk Eko Hari Basuki
Research Journal of Life Science Vol 4, No 3 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Yeast which has a fermentative type can ferment alcohol, which break down the sugars into alcohol, lactic acid and gas. This yeast’s type is needed in biofertilizer process production. The purpose of this study was to observed the potential in ethanol production of the soil wild yeast that isolated from Jember-East Java, and determine the concentration of sugar product as well as the best time of ethanol fermentation. Our previously observation have found 113 yeast species. Then we select them based on fermentative ability and found five best species. These five species then has determined it’s sugar product as well as the best time of ethanol fermentation and choose two best. We have counted the level of ethanol production using glucose medium of 10%, 15% and 20% of these two best species. The results showed that Candida sp. 1 (species number-1) and Candida sp. 3 (species number-3) has a better potential to produce ethanol. The highest ethanol content was produced by Candida sp. 1 (56%) while by Candida sp. 3 (70%). While the optimum time for the ethanol fermentation process Candida sp. 1 and Candida sp. 3 is 24 hours.

Catechins of GMB-4 Clone Inhibits Adipogenesis Through PPAR-γ and Adiponectin in Primary Culture of Visceral Preadipocyte of Rattus Norvegicus Wistar Aswaty Nur; Retty Ratnawati; Diana Lyrawati
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Catechins of green tea (Camelia sinensis) GMB4 clone may serve as a potential therapeutic antiobesity agent, probably through its effects on preadipocytes. Thus, to evaluate such antiobesity effects, we performed series of in vitro experiments using primary cultures of visceral preadipocytes from Rattus norvegicus strain Wistar. Quick Cell Proliferation assay, Oil Red-O staining, ELISA and immunocytochemistry were used to determine the effects of 25 µM, 50 µM, 75 µM, and 100 µM catechins on primary culture of preadipocytes, particularly on cell viability and differentiation as well as on expression of relevant obesity genes i. e. PPARγ and adiponectin levels. The results showed that there were no significant differences on preadipocytes viability among control and catechins treatments except in cells treated with 50 µM catechins (means±SD=128±2.47) which resulted 28% higher viability than control (p= 0.037). Catechins inhibited preadipocytes differentiation into adipocytes, at 100 µM up to 78% lower than control. The level of PPARy apparently was reduced by catechins, but statistically significant only at 75 μM (p= 0.029). In contrast, the adiponectin level on preadipocytes increased by catechins at 75 µM and 100 µM (0.786±0.126 and 0.673 ±0.319 ng/ml; control: 0.077±0.017, p ≤0.01). In conclusions, our data revealed that desired antiobesity effects of catechins of green tea GMB4 clone on visceral preadipocytes were concentration-dependent; at dosage 50 µM catechins enhanced cell viability; at more than 75 µM inhibited differentiation of preadipocytes and was associated with lower PPARy and higher adiponectin levels.

Design Development of Sprinkler Irrigation System To Protect The Failed Harvest of Citrus “Keprok 55” In Dry Season at Selorejo, Dau, Malang Bambang Suharto; Liliya Dewi Susanawati
Research Journal of Life Science Vol 3, No 1 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This study is a part of ongoing research to mainstay citrus productivity in Malang region. Therefore, the specific aim of this study is developing the design of sprinkle irrigation in field. Selorejois central location of citrus production called “KEPROK 55” in Dau-Malang. The cultivation requires maintenance of soil and plants, in addition the lower of citrus production especially in dry season thus indispensable an effort to increase the production. Application of technology using sprinkle irrigation is expected to create the increasing of Citrus production. Result on research study show that the coefficient of uniformity tends to have same value on all the treatment that is above 98%, where the average value of the coefficient of uniformity was 98.8%. The highest value of the coefficient of uniformity achieved at combination of treatment P1T1 (pressure of 1 bar with a riser pipe 50 cm height) that is equal to 99.78%. While the coefficient of uniformity was lowest at the combination of treatment P2T3 (pressure of 1.5 bar with a riser pipe 150 cm height) by 96.6%. The pressure effected to the coefficient of uniformity indicates that the greater pressure exerted, then the coefficient of uniformity will be higher. It indicated at the treatment of pressure P1, P2, and P3 (1 bar, 1.5 bar and 2 bar) that have the average coefficient of uniformity of 98.53%, 98.27%, and 99.64%.

Antihypertensive Effects of Black Cincau (Mesona palustris BL) Effervescent Powder and Malondialdehyde Concentration on Wistar Rats as a Hypertensive Model Tri Dewanti Widyaningsih; Novita Wijayanti; Dian Handayani; Guntur Prasetyo
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This study investigated the effects of Black cincau (Mesona palustris BL) effervescent powder (BCEP) on systolic blood pressure and malondialdehyde concentration in hypertensive rats. BCEP were orally administered to hypertensive rats for 4 weeks, and antihypertensive effects were determined. Rats were given 630 mg/kg, 1.260 mg/kg and 1.890 mg/kg BCEP on dose group 1, 2, 3 respectively. These groups were compared with a negative control group, hypertensive control group and Captopril control group. These results showed that systolic blood pressure and MDA concentrations of rats dosed with BCEP were significantly different (α=0,01) to those of control rats. Rats dose third group with of 1.260 mg/kg showed the highest percentage of lowering blood pressure. The rat dose third group had highest percentage decreased in systolic blood pressure (43,97%) and in MDA concentration (68,77%).Key words : Antihypertensive; Black Cincau (Mesona palustris BL) Effervescent Powder; Malondialdehyde

The Effects of Fiddler Crabs (Uca sp) on C/N Ratio and Redox Potential of Soil in Mangrove Ecosystems Mulyanto Mulyanto; Herwati Umi
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Research has been done in Ketapang mangrove area of Probolinggo city in months of September-November 2015. The objectives are to observe the fiddler crab community stucture and to analyze the effects of fiddler crabs on C/N ratio and redox potential of soil in mangrove ecosystems. The samples of fiddler crabs were taken during the low tides at 4 station (20 transects with sizes of 1 m2). Data of the fiddler crabs were measured from the soil digging insides the transect. The soil samples were taken from these crab holes wall (at the surface and at the depth of 20 cm), under the holes at the depth of 40 cm as well as from the locations that undwells by these animals at the same depth. The fiddler crab identified are U. Triangularis between 2 – 6 ind/m2, U paradussumieri 1 – 3 ind/m2, U perplexa 14 – 32 ind/m2, U dussumieri 12 – 27 ind/m2 and U. Tetragonon 3 – 6 ind/m2. The diversity is moderate (H = 1.7) and the dominance index was low (C = 0.37). C/N ratio soil were inhabited by fiddler crab between 6 – 14, the undwelled area were 14 – 20. Soil C/N ratio was inhabited by the fiddler crab at the surface and depth of 20 cm in average of 9 cm while at 40 cm in avergae of 12. The low of C/N ratio at surface and depth of 20 cm causing the organic matter turnover faster because the high nitorgen content. Soil potential redox (Eh) the undwelled areas was found –0.647 mV, meanwhile the soil Eh in the dwelled areas was positive (0.68 till 0.87 mV). This mean, the decpmposition was occured during aerobic condition and will produce untoxic subtances.

The Effect of Photoperiod to Break Dormancy of Porang’s (Amorphophallus muelleri Blume) Tuber and Growth Serafinah Indriyani; Wahyu Widoretno
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aim of the research was to obtain the method of porang’s tuber dormancy breaking. The source of porang’s tuber were obtained from Rejosari Village, Bantur Subdistrict, Malang Regency. The diameter and weight of porang’s tuber were 5-7 cm and 100-150 g respectively. The research design was Completely Randomized Design. Porang’s tubers were storaged in the photoperiod cabinet during one month. The levels of photoperiod were 0, 8, 12, 16, 20, and 24 hours/day. The light intensity of TL lamp was 400 lux. As a control it was used porang’s tuber that it was placed in the dark cabinet without light. Repetition was four times. The success of dormancy breaking was observed through the tuber capasity to grow that it was identified by bud emerged. Data were analyzed by ANOVA that it was continued by Duncan test (α = 0.05). The results showed that photoperiod 8, 12, 16, 20, and 24 hours/day during one month could stimulate porang’s tuber dormancy breaking. Photoperiod 16 hours/day emerged the height of buds 4.97 ± 1.20 mm and the diameter of buds 7.28 ± 1.22 mm at photoperiod 24 hours/day. The growth of porang’s tuber which is treated by photoperiod during one month were significantly different with control. Photoperiod 16 hours/day emerged the height of plants and the diameter of petiole were 106.38 ± 15.11 cm and 2.90 ± 0.29 mm respectively, while the width of canopy was 72.50 ± 22.17 cm at photoperiod 24 hours/day. Besides, the diameter and weight of harvest tuber were 7.93 ± 2.20 cm and 383.20 ± 23.58 g. The weight of harvest tuber increased 255% from the early weight of tuber. Photoperiod treatment promote breaking of porang’s tuber dormancy.

The Effect of Niosomal System (Span 60-Cholesterol) on Diclofenac Sodium Preparation Characteristics and Diclofenac Sodium Preparation of Hydroxypropyl Cellulose Gel Base (HPC) Adeltrudis Adelsa Danimayostu; Esti Hendradi; Tutiek Purwanti
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Diclofenac sodium is a lipophilic drug. That characteristic makes it difficult to disperse well in hydrophilic gel base. The niosome with its vesicle system could overcome that low dispersibility. It affects penetration by reducing water loss in trans-epidermal layer and change lipid bilayer conformation. The present study was designed to investigate the effect of niosomal system (Span 60–Cholesterol) on preparation characteristics and diclofenac sodium penetration of hydroxypropyl cellulose (HPC) gel base. We examined three different formulas in HPC gel base. Formula III was made in niosomal system. Preparation characteristics were evaluated with organoleptic and acidity tests. Drug penetration was checked using apparatus 5-paddle over disk and 0.45 μm Milipore membrane impregnated with isopropyl myristate. The solution is phosphate buffer saline pH 7.4±0.05 in temperature 37±0.5°C. All of the data were evaluated based on one way ANOVA and continued with HSD test. It was concluded that niosomal system (span 60-cholesterol) has an influence in increasing pH value and penetration (based on flux value and permeability) of diclofenac sodium in HPC gel base.

Eficacy of Water Clover Extract (Marsilea crenata) Against Blood Estrogen Progesteron Balance, Blood Calcium Levels and Impact on Dense of Bone Tissue of Rat (Rattus novergicus) Pratiwi Trisunuwati
Research Journal of Life Science Vol 4, No 1 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The potential of phytoestrogen for the stabilized of low condition blood estrogen hormone much more important to be sudy. So the aim of this reseach were to find an natural herbal active compounds Water Clover Extract (Marsilea crenata) which contain phytoestrogen isoflavones as an alternative invasive treatment against menopause women and subestrus in dam of livestock. The other advantage is that could be potential to develop as alternative compound against animal with low level of blood calcium that relevance on progesterone level, because there was some rising of exogen phytoestrogen. This research is true experimentally post test control design based on completely randomized design. The old females white rat (Rattus norvegicus), divided into 5 groups challenges with 2 ml each Marsilea crenata extract in different concentration such as 6.25%, 12.5%, 25%, 50% given by gastric sonde along 23 days, compare negative control group. Level of blood calcium using SSA showed increase in all the treatment (P<0,05) started on 12,5%, lower the progesterone and raised estrogen hormone as natural balance. To find about effect of Calcium toward osteogenesis used to determine the description of histopathologis (HE) bone tissue that impact on bone density. Histology os humerus showed that have impact on dense of bone tissue. The conclusion of this research were Marsilea crenata juice plays a role in increasing toward blood calcium levels and surely increase bone density in animal laboratory (Rattus norvergicus), supporting by blood estrogen progesterone balance. The suggestion was Marsilea crenata extract could be potential as a substance to increase estrogenic progesterone balance and rise blood calcium both in human and animal.

Chemical Characteristics of Biscuit Substituted by Modified Cassava Product (Mocap) Flour from High Cyanide Variety of Cassava Harijono Harijono; Maimunah Hindun Pulungan; Jhauharotul Muchlisyiyah; Erna Triyas Tanti; Teti Estiasih
Research Journal of Life Science Vol 4, No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Cassava (Manihot esculenta Cranzt) is easily cultivated and an important source of carbohydrates. Currently, only a certain type of low cyanide cassava has been utilized for food product and intermediate product as flour. Processing of high cyanide cassava through submerged and solid-state fermentation simultaneously into mocap (modified cassava product) flour has successfully lowered the cyanide into a safe level for consumption. The application of mocap flour for biscuit is important to know the characteristic changes that occur due to substitution of mocap flour. Variations of wheat flour: mocap flour of 40:60, 30:70, 20:80, 10:90, and 0:100 were used in the preparation of biscuits. The resulting biscuits were analyzed for their chemical characteristics including moisture, protein, fat, starch, amylose, crude fiber, reducing sugars, and cyanide compounds. The cyanide compounds comprised of total cyanide, HCN, and cyanogenic glycosides. Substitution of wheat flour by mocap flour in biscuit making produced the changes of biscuit characteristics. Protein, moisture, starch, amylose, fat, and reducing sugar content was decreased by increasing substitution of mocap flour. Meanwhile, crude fiber and cyanogenic compounds increased by increasing mocap flour substitution. All biscuits with variation of mocap flour substitution were safe to consume because of low level of cyanides.

Page 7 of 21 | Total Record : 202

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Kuswanto

Contact Email
rjls@ub.ac.id

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+62341 575825

Journal Mail Official
rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA