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INDONESIA
AGRISAINS
Published by Universitas Tadulako
ISSN : 14123657     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Ilmiah AgriSains terbit dalam tiga volume dalam satu tahun yakni bulan April, Agustus dan Desember. Berisi artikel ilmiah dalam bidang agrisains. Penerbitan jurnal ini bertujuan meningkatkan kualitas, kuantitas, media komunikasi dan penyebaran informasi dalam bidang agrisains.
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 21, No 1 (2020)" : 7 Documents clear
Evaluation of CpG DNA for Increasing Immune Gene Expression in Grouper (Epinephelus fuscoguttatus) Jamaluddin, Ruqayyah; Yusuf, Sunarti; Nursida, Nur Fajriani; Suryahman, Agus; Tahya, Akbar Marzuki
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

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Abstract

CpG DNA is a kind of DNA synthetic immunostimulant containing CpG motifs consisting of short sequences containing one or more CpG motifs which act as a danger signal to the natural and innate immune systems. This study aimed to analyze the ability of CpG DNA to increase Interleukin 1β (IL1β) gene expression in tiger grouper so that it could be used as immunostimulant. Analysis of the expression level was carried out by extracting RNA- from the cephalic kidney which was treated with CpG DNA or with PBS as a control. Complementary DNA (cDNA) synthesis was carried out using the Ready To Go You Prime First Strand Beads (GE Healthcare) kit using the RT-PCR technique. The PCR product was analized by measuring the photostimulated luminescence value using UN SCAN IT software based on the thickness of the DNA fragments. The gene expression level and photostimulated luminescence value of the IL1β gene were higher than that of the PBS treatment used as a control. The luminescence value of the photostimulated IL1β gene in CpG DNA treatment was 113684 while the PBS treatment as a control was only 95610.
Frequency of Feeding on Body Weight and Chemical Levels of Local Goat Blood Hamid, Padang; Zainal, Zainal; Nirwana, Nirwana; Mustafa, Mustafa
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

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Abstract

This study aims to determine the body weight gain in the consumption of dry matter, the efficiency of the use of dry matter, blood glucose levels and blood urea levels of local goat which were fed with different frequencies. The test organisms were 15 local female goats aged 10 months and body weight of 14–20 kg. This research was an experimental study using a completely randomized design (CRD) with three treatments and 5 replications. The treatments tried were: P1 = Feeding 1.5% concentrate and 1.5% dry matter forage given once at 07.00 (UTC+8) based on body weight; P2 = 0.75% concentrate and 0.75% dry matter forage given at 07.00 (UTC+8), 13.00 (UTC+8) given 0.75% concentrate and 0.75% dry matter forage based on body weight; P3 = Feeding 0.5% concentrate and 0.5% forage dry matter is given at 07.00 (UTC+8), and at 13.00 (UTC+8) given 0.5% concentrate and 0.5% and at 19.00 (UTC+8) given 0.5% concentrate and 0.5% for dry matter based on body weight. The results of this study indicated that the frequency of feeding did not have an effect on dry matter consumption and blood urea levels, however, 2 times feeding significantly increased body weight gain, ration efficiency and blood glucose levels of local goat.
Different Preliminary Feed on The Response of Growth And Sustainability of Betta Fish Larvae (Betta Splendens) Yohana, Yohana; Tantu, Fadly Y; Madinawati, Madinawati
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

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Abstract

Some problems in Betta splendens cultivate activities were slow growth and mortality rates still high in the larval stage. Generally, Betta splendens larvae require a feed type based to the larval mouth. Some types of feed that can be given for larvae were Infusoria and yolk suspension. The study aims to find out the growth and survival rate of Betta splendens giving different first feeding. The study was conducted in January-February 2019 at Laboratory of Water Quality and Aquatic Biota, Faculty of Animal Husbandry and Fisheries, Tadulako University. The study used a Completely Randomized Design consisting of 3 treatments: A (infusoria); B (egg yolk); C (infusoria and egg yolk). Data were analyzed with ANOVA. The results showed that giving different first feeding did not significantly affect the growth of Betta splendens. The highest weight in the treatment of infusoria feed and the highest percentage of survival rate in the treatment of egg yolk.
Phylogenetic Analysis of Mud Crab Scylla olivacea with Several Crustacean Based mRNA Encoding FAMeT Yusuf, Sunarti; Tahya, Akbar Marzuki; Jamaluddin, Ruqayyah
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

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Abstract

Previous studies have succeeded in isolating, and the basic sequence of mRNA encoding FAMeT Scylla olivacea. Several studies have noted that the FAMeT encoding mRNA sequence has differences in crustacean species. The aim of this study was to determine the relationship between S. olivacea and other crustaceans based on the FAMeT encoding mRNA sequence. The similarity of the FAMeT encoding mRNA sequences showed that several crustaceans had similarities to the FAMeT mRNA sequences of S. olivacea. The phylogeny tree analysis showed the existence of 2 clusters of the relationship between the mRNA encoding FAMeT of S. olivacea on the same branch as 2 other crab species: S. serrata and S. paramamosain. It was also found in Portunus trituberculatus and P. pelagicus and river prawn Macrobrachium rosenbergii. These findings indicated that the S. olivacea crab was monophyletic with 2 other mangrove crabs, namely the crab and the river prawn M. rosenbergii. So that describes a very close kinship relationship genetically. Meanwhile, the second branch consists of crustaceans such as Homarus americanus, Cherax quadricarinatus, Fenneropenaeus merguiensis, Metapenaeus ensis and Litopenaeus vannamei.
Comparison of Agronomic Attributes Two Types of Panicum Grass on The Pot Trial Tarsono, Tarsono
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

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Abstract

A pot trial aimed comparing several aspects of agronomical atributes of two types of Panicum has been carried out from August to September 2018 in the greenhouse of Tadulako University, Palu. A completely randomized design (CRD) of two treatments which were repeated 10 times was used in this study. The variables observed included plant height, number of tillers, length of leaf-sheath, length of leaf-blade, leaf width, and dry matter production. The data collected was analyzed for variance using the SX program, with followed-up by the least significance difference test (P=0.05) for variables that were significantly affected by treatment. The results showed that Panicum sarmentosum grass has a similarity to Panicum maximum in terms of number of tillers, leaf-sheath length, leaf width, and plant height (P>0.05), except for leaf-blade length, and dry matter production (P<0.05).
Survival Rate and Growth of Pterapogon kauderni Larvae Given Different Feed Types Safir, Muhammad; Tobigo, Desiana Trisnawati; Mangitung, Septina F; Sambaeni, Daniel; Ryaldi, Muhammad; Adam, Rizilvana Datu; Zainab, Zainab; Husain, Husain
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

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Abstract

This research was conducted to determine the type of feed that provides high survival and growth in Pterapogon kauderni larvae after release from the mother's mouth. The study was designed using three treatments including; commercial feeding (A), shrimp/Acetes (B), and rotifer/Rotifera sp. (C). Each treatment was given three replications. Feed is given three times a day (morning, afternoon and evening) by at-satiation. Maintenance time is carried out for one week. The analysis showed that the highest daily growth rate occurred in treatment B (1.39 ± 0.92%), and was significantly different (P <0.05) with treatment A (0.90 ± 0.02%). The highest increase in biomass occurred in treatment C (0.005 g) compared to other treatments (A = 0.003 g, B = 0.003 g). Survival for all treatments did not differ significantly (P> 0.05). In conclusion, the type of feed that provides the highest survival and growth in P. kauderni larvae after release from the mother's mouth is rotifers.
Effect of Probiotic Addition EM-4 (Effective Microorganism-4) on Feed to Growth, Food Conversion Ratio and Survival Rate of Common Carp Larva Cyprinus carpio L. Abdillah, Abdillah; Madinawati, Madinawati
AgriSains Vol 21, No 1 (2020)
Publisher : FAPETKAN UNTAD

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Abstract

The problem that often becomes an obstacle in common carp culture is that the artificial feeding of common carp seeds has not yet had an optimal effect on survival and growth rates. due to the digestive system of seeds has not morphologic or physiologic differentiated, so the ability of seeds to digest feed still requires the addition of supplements such as probiotics. The research aims to analyze the effect of EM-4 probiotics addition on feed to growth SGR, FCR and survival rate of common carp seeds. The study was conducted in April to May 2019 at the Water Quality and Aquatic Biota Laboratory, Tadulako University. The study used a Completely Randomized Design consisting of 5 treatments and 4 replications. The treatments in this study were variations in the dose of EM-4 mixed addition in commercial feeding: A (0 ml/kg of feed); B (2 ml/kg of feed); C (6 ml/kg of feed); D (10 ml/kg of feed); E (14 ml/kg of feed). Data analysis used ANOVA and continued with LSD tests. The results showed that the use of EM-4 probiotics in feed significantly affected (P <0.05) on SGR, FCR and survival rate. Treatment D (10 ml / kg of feed) was the best treatment that resulted in SGR 0.014%, absolute weight growth of 7.775 g, FCR of 0.350 and 95% survival rate.

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