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Cantilever Bridge Design as Esthetic Restoration on Post Endodontic Treatment: Case Report Fatmawati, Dwi Warna Aju
The Indonesian Journal of Dental Research Proceeding Book
Publisher : The Indonesian Journal of Dental Research

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In dentistry, aesthetic and cosmetic had become patient demands, because it was related with beauty. In aesthetic restorative and cosmetic treatment, dentists were not only concerned in beauty but also must consider function of restoration. Thus, dentists must select materials and designs that had ability on occlusion and mastication bearing for long time in oral cavity. Dentures or restoration treatment that had similar color with tooth was known for a long time, because it can improve aesthetic and self-confidence. The aim of this case report was to explore aesthetic restoration used in cantilever bridge on post endodontic treatment subject. This bridge can be used as a more simple alternative treatment for subject whose restorative was broken and used partial denture.Â

Dynamic Changes of Sp6 Transgene Expression in Dental Epithelial Cells during Long-term Culture Trianna W. Utami; Keiko Miyoshi; Hiroko Hagita; Ryna D. Yanuaryska; Taigo Horiguchi; Takafumi Noma
The Indonesian Journal of Dental Research Vol 1, No 3 (2011)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

To investigate the function of specificity protein 6 (SP6) transcription factor by gain-of-function procedure, we established cytomegalovirus (CMV) promoter-driven Sp6 stable transformants, C9 cells, using dental epithelialderived cells. Initially, C9 cells produced a significant amount of SP6 protein. However, SP6 expression was reduced in these cells upon long-term culture. We could detect Sp6 transcripts in C9 cells by RT-PCR throughout the passages, although the CMV promoter is known to be epigenetically silenced. We recently found that SP6 was a short-lived protein that was degraded by a ubiquitin-independent proteasome pathway, although it is yet unclear how Sp6 expression was regulated during culture. Thus, we studied the possibility of epigenetic regulation of Sp6 expression. Comparative analysis of endogenous and exogenous Sp6 mRNA expressions demonstrated the specific down-regulation of exogenous Sp6 mRNA levels during culture passages. A DNA methyltransferase inhibitor, 5-Aza-2'-deoxycytidine (5AC), and a histone deacetylase inhibitor, valproic acid (VPA), enhanced or induced SP6 protein expression up to passage 28 without enhancing the mRNA level. The dramatic up-regulation of exogenous Sp6 mRNA was uniquely observed only at passage 50 by 5AC or VPA treatment. These findings indicate that multiple epigenetic regulatory mechanisms operate to fine-tune Sp6 expression during long-term culture.

Systemic IL-1β and TNF-α Productions of E. coli Lipopolysaccharide-Induced Periodontitis Model on Rats Alma Linggar Jonarta; Widya Asmara; Indwiani Astuti; Regina TC Tandelilin
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Periodontal disease, a common inflammatory oral disease involved periodontal tissues, has been linked with the evidence of some systemic disorders. Recently, periodontal disease has been suspected as a trigger of systemic disorders. Penetration of bacterial products, such as lipopolysaccharide (LPS) may reach into deeper periodontal tissues. Therefore there may affect systemic blood and cytokines production. Interleukin-1β (IL-1β) and Tumour Nuclear Factor-α (TNF-α) are known as pro-inflammatory cytokines. The production of systemic IL-1β and TNF-α of E. coli lipopolysaccharide-induced periodontitis model on rats was investigated in this research. Fifteen male Wistar rats, aged 6-8 weeks used for this study were divided into 3 groups. For group 1 and 2, silk ligature 3/0 were inserted in interdental area between upper right molar 1 and 2. First and second group received solution containing 10μg/ml and 1mg/ml E. coli lipopolysaccharide, respectively, mixedwith 2% carboxymethylcellulose (CMC) diluted in 100μl of phosphate buffer saline (PBS). The solution was topically applied on gingival tissues around the gingival sulcus, a single topical application of solution onceper 2 days for 14 days. Untreated subjects were used as negative control. On day 15, the blood was collected from vena orbitalis, and rats were sacrificed. The blood serum of each group was divided into 2 groups andcultured for 4 hours with or without 20μl of 100ng/ml of E. coli LPS. ELISA techniques were used to measure the cytokine productions of the supernatant. The data was analysed using Repeated Measure ANOVA. This study showed that there was a significant increase of IL-1β production on low dose of LPS compared to control and high dose of LPS groups (p<0.05). Whereas TNF-α not significantly showed increasing trend. The increasing trend of pro-inflammatory cytokine productions, such as IL-1β and TNF-α, on LPS-induced periodontitis model in this experiment supports the previous studies about the contribution of periodontal disease in the pathogenesis of systemic diseases.

Anti-Inflammatory Activity of Ethanol Extract of Cashew Stem Bark (Annacardium Occidentale L.) on Rat Paw Edema Induced by Carrageenan Harsini, Harsini; Sutardja, Iwa; Martono, Sudibyo; Sunarintyas, Siti; Sudarsono, Sudarsono
The Indonesian Journal of Dental Research Proceeding Book
Publisher : The Indonesian Journal of Dental Research

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19.124 KB)

Introduction: Cashew stem bark (Anacardium occidentale L.) was traditionally used to cure inflammation in the oral cavity. Phenolic substances such as phenol and anacardic acid that have anti-inflammatory effect was found in cashew stem bark. The aim of this study was to investigate the anti-inflammatory activity of the ethanol extract of cashew stem bark and indometazine as Non-steroid anti-inflammatory drug. Materials and Methods: Cashew stem bark was collected from Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta, Indonesia. Extraction was done by maceration method using ethanol as solvent. Anti-inflammatory activity of 40 mg/kg bw, 80mg/kg bw, 160mg/kg bw dosage of cashew stem bark extract was monitored and indometazine 10 mg/kg bw was used as positive control. Edema volume determination on rat paw was counted as area under cure (AUC) value and anti-inflammatory percentage. Result: This study result showed that total phenolic content on cashew stem bark was 12.25 Â± 0.26% w/w gallic acid equivalent (GAE). The anti-inflammatory activity of cashew stem bark extract in this study were 9.985Â±6.483% for 40mg/kg BW, 15.576Â±6.754% for 80mg/kg bw, 25.87Â±19.7% for 160mg/kg bw and 56.85 Â±15.52% for Indometazine 10 mg/kg bw. Analysis of Variance (ANOVA) method was applied on the results and showed significant anti-inflammatory activity of ethanol extract on cashew stem bark (p<0.05). Conclusion: In conclusion, ethanol extract of cashew stem bark has anti-inflammatory activity. However, itsâ activity is lower than indometazine.

The Effect of Diabetes Mellitus on the Thickness of Gingival Junctional Epithelium (Study in the Experiment of Caspase-3) Abdelaziz Eljawadi; Totok Utoro; Nunuk Purwanti
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65708

Diabetes mellitus (DM) is a metabolic disorder manifested by abnormally high levels of blood glucose, resulting in hyperglycemia that affects the oral cavity, leading to periodontitis. The junctional epithelium (JE) is the epithelial component of the dento-gingival unit that is in contact with the toothsurface. Apoptosis and proliferation of JE are essential to maintenance JE thickness. Apoptosis is programmed cell death that can be triggered by various signals and is characterized by well-defined morphologic changes and biochemical features. Caspase-3 is involved in the underlying mechanisms of apoptosis, and the activation of caspase-3 is considered to be the final step in many apoptosis pathways. Purpose of this study was to investigate the effect of DM on the expression of caspase-3 and the thickness of JE. Sixteen male Sprague-Dawley rats were used and divided equally into two groups: the diabetic group that injected intraperitoneal by streptozotocin (STZ) and negative control group. Measurements of blood glucose levels were analyzed before and at 2, 4 weeks after STZ injection. In addition, JE thickness and expression of caspase-3 were examined after 2 and 4 weeks. JE was stained by hematoxylin-eosin (H&E) staining for thickness measurement and the immunohistochemistry by using the anti-caspase-3 antibody for caspase-3 expression measurement and examined under light microscope. The results of the present study showed that a decrease of JE thickness and increase of caspase-3 expression were obtained while increasing the diabetic duration. Two ways Anova and Least Significant Difference (LSD) tests indicated a significant difference of JE thickness and caspase-3 expression between all groups except in diabetic group after 2 and 4 weeks. Also, caspase-3 expression in diabetic group after 2 and 4 weeks (P > 0.05) were not significantly different. It can be concluded that diabetes mellitus (DM) affected on the thickness and caspase-3 expression of JE. Furthermore, the results suggest that high expression of caspase-3 was associated with the diabetes-induced apoptotic cell-death resulting in reduction of JE thickness.

Influence of Bacterial Endotoxin on Mucosal Immune Response to Phosphorylcholine Sapta Adisuka Mulyatno; Kosuke Kataoka; Makoto Fukui; Tselmeg Baatarjav Rita Cristina Orihuela Campos; Hiro-O Ito
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65711

Bacterial lipopolysaccharide (LPS), a component of the outer membrane of gram-negative bacteriathat initiates inflammation by activation innate immune responses through Toll-like receptor 4(TLR4). However, the influence of LPS on the mucosal immune reactions remains to be addressed.This study was examined the effect of LPS in nasal vaccination model. BALB/c and C57BL/6 micewere nasally immunized with keyhole limpet hemocyanin (KLH) conjugated with hapten phosphorylcholine (PC) or trinitrophenol (TNP) with LPS as a mucosal adjuvant, in the presence orabsence of cholera toxin (CT). The antibody titers were measured in serum, saliva, and nasal washfluids by an enzyme-linked immunosorbent assay (ELISA) in IgM, IgG, and IgA isotype-specificmanner. The epitope-specific antibody production induced in blood and mucosal fluid was furtherenhanced by LPS for all isotypes examined. Besides, LPS, which has rarely been regarded as a mucosal adjuvant, was tested for its adjuvanticity by comparing the nasal immunization with PC-KLH plus LPS or with PC-KLH plus CT. LPS showed high adjuvanticity almost equal to CT. Possible differences of LPS from CT as a mucosal adjuvant remains to be elucidated.

The Effect of Toothbrushing Duration on Nickel Chromium Alloy Wear Sri Budi Barunawati; Siti Sunarintyas; Rini Dharmastiti
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65713

Nickel-chromium alloy is a preferred material for fixed partial denture due to its low cost as well as good physical and mechanical properties. Tooth brushing using toothpaste produces abrasion on restoration, especially in a long period. This study aimed to observe the effect of toothbrushing duration on the wear of nickel-chromium alloy. Twenty four specimens of nickel-chromium alloy (Metal 4all, Ivoclar, USA) in 30X15X1mm3 dimension were treated using tooth brushing simulation machine (wear test machine, pin on plate unidirectional movement type) and toothpaste (modification of Balsam formula). The brushing durations were 30.9, 77.25, 123.6, and 154.5 hoursas the simulation of 2, 5, 8, and 10 years tooth brushing. Surface roughness and weight difference as abrasion indicator were measured and analyzed using one-way ANOVA followed by LSD test. Tooth brushing duration of 2, 5, 8, and 10 years increased nickel-chromium alloy surface roughness (Ra) by 0.16, 0.39, 0.43, and 0.56µm with weight loss of 8%, 15%, 23%, and 32 %, respectively. These differences were statistically significant (p <0.05). The result of LSD test showed a significant effect (p <0.05) between groups of toothbrushing duration. The increase of surface roughness affects the increase of wear volume of nickel-chromium alloy indicated by R = 0.11 for brushing duration of 2, 5, 8, and 10 years. The conclusion of this study was 10 years tooth brushing promoted wear on nickelchromium alloy, whichwas indicated by the increase in surface roughness and weight loss.

Lithium Disilicate Glass-Ceramic Surface Appearance after Acid Surface Treatment Sana Mohammed Alrefae; Siti Sunarintyas; Widowati Widowati
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65714

Dental ceramics are widely used and studied in dentistry because they are durable, aesthetically appealing and provide excellent biocompatibility. All glass-ceramic surfaces must be etched using hydrofluoric acid (HF) to increase surface roughness determined by roughness average (Ra) before cementation to a tooth surface. This research aimed to analyze the effect of hydrofluoric acid surface treatment concentration on the surface roughness of lithium disilicate glass ceramic. A total of fifteen discs of lithium disilicate glass ceramic were prepared (10mm in diameter and 1mm in thickness). Specimens were divided into 3 groups (n=5). Group A (control) was no treatment, group B was etched by 5% HF for 2 min, and group C was etched by 9.5% HF for 2 min. The etched surfaces were observed by Scanning Electron Microscope (SEM). The measurement of the Ra of the lithium disilicate glass ceramic was determined with surface roughness tester machine. The results showed that the means of Ra (μm) were 0.096±0.009μm, 0.608±0.054μm, and 0.892±0.101μm in group A, B, and C, respectively. The one-way ANOVA showed there was an effect of hydrofluoric acid surface treatment concentration on the surface roughness of the lithium disilicate glass ceramic. The post hoc test showed there was a difference of Ra (μm) among the experimental study groups (p<0.05). In conclusion, the concentration of hydrofluoric acid influences Ra of lithium disilicate glass ceramic.

Identification of Veillonella spp. on Tongue Plaque and Saliva Using Real-Time PCR Karina Dhaniarti; Fathia Agzarine Deandra; Ariadna A Djais; Boy M Bachtiar
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65715

Veillonella spp., Gram-negative obligate anaerobic cocci bacteria, amounts to 3% in the oral cavity, relies on the fermentation of lactate as a carbon and energy source for growth. The bacteria are considered anti-cariogenic as they metabolize lactic acid into propionic acid which increases oral environment’s pH and reduces demineralization rate of tooth structure. Identification of Veillonella spp. using traditional methods is difficult due to the lack of conventional phenotypic and biochemical tests. Thus, the biomolecular methods are suitable for the specific detection and identification of Veillonella spp. One of the biomolecular methods that can be used is real-time Polymerase Chain Reaction (PCR), which the results can be qualitative and quantitative. This study aimed to identify Veillonella spp. in tongue plaque’s and saliva’s samples using Real -time PCR. The DNA of Veillonella spp. derived from 36 samples, 18 samples of tongue plaque and 18 samples of saliva, were extracted using a freeze-thaw method and then quantified by real-time PCR using forward primer 5’-CCG TGA TGG GAT GGA AAC TGC-3’ and reverse primer 5’-CCT TCG CCA CTG GTG TTC TTC-3’. Veillonella spp. in 18 samples of tongue plaque was 3,06 x 107 CFU/ml and in 18 saliva samples was 1,51 x 105 CFU/ml. It was concluded real-time PCR can detect Veillonella spp. from all tongue plaque’s and saliva’s samples.

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Journal Mail Official
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Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA