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Expression of MRP8/MRP14 mRNA in Monocytes of Periodontitis: Comparison between Diabetic and Non Diabetic Patients Ahmad Syaify; Sri Budi Barunawati; Suryono Suryono; Marsetyawan HNES
The Indonesian Journal of Dental Research Vol 1, No 1 (2010)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

The severity of periodontitis on patients with type 2 Diabetes Mellitus patients was strongly thought caused by decreasing of leukocytes function such as monoctyes and neutrophils. In our previous research it was found that calprotectin (MRP8/MRP14) level in leukocytes of periodontitis patients with type 2 DM was higher than periodontitis in non DM. The aim of this study was to determine calprotectin (MRP8/MRP14)mRNA expression in human monocytes of periodontitis patients with type 2 DM and without DM. Monocytes were isolated from the peripheral blood of periodontitis patients with uncontrolled type 2 DM, controlled type 2 DM, and non DM. The expression of total RNA calprotectin (MRP8 and MRP14) were detected by RTPCR using GAPDH as the innate control. It was observed that the value of MRP8/MRP14 mRNA expression DM patients were higher than non DM, and the highly significant increase expression (p<0.05) was on the uncontrolled type 2 DM. The basal level of MRP8/MRP14 expression increased in monocyte of periodontitis and type 2 DM patients compared with non diabetes subjects. It was suggested that high basal level MRP8/MRP14 has role in the regulation of severity periodontitis with diabetes mellitus.

The Effect of Toothbrushing Duration on Nickel Chromium Alloy Wear Sri Budi Barunawati; Siti Sunarintyas; Rini Dharmastiti
The Indonesian Journal of Dental Research Vol 1, No 1 (2012)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/theindjdentres.65713

Nickel-chromium alloy is a preferred material for fixed partial denture due to its low cost as well as good physical and mechanical properties. Tooth brushing using toothpaste produces abrasion on restoration, especially in a long period. This study aimed to observe the effect of toothbrushing duration on the wear of nickel-chromium alloy. Twenty four specimens of nickel-chromium alloy (Metal 4all, Ivoclar, USA) in 30X15X1mm3 dimension were treated using tooth brushing simulation machine (wear test machine, pin on plate unidirectional movement type) and toothpaste (modification of Balsam formula). The brushing durations were 30.9, 77.25, 123.6, and 154.5 hoursas the simulation of 2, 5, 8, and 10 years tooth brushing. Surface roughness and weight difference as abrasion indicator were measured and analyzed using one-way ANOVA followed by LSD test. Tooth brushing duration of 2, 5, 8, and 10 years increased nickel-chromium alloy surface roughness (Ra) by 0.16, 0.39, 0.43, and 0.56µm with weight loss of 8%, 15%, 23%, and 32 %, respectively. These differences were statistically significant (p <0.05). The result of LSD test showed a significant effect (p <0.05) between groups of toothbrushing duration. The increase of surface roughness affects the increase of wear volume of nickel-chromium alloy indicated by R = 0.11 for brushing duration of 2, 5, 8, and 10 years. The conclusion of this study was 10 years tooth brushing promoted wear on nickelchromium alloy, whichwas indicated by the increase in surface roughness and weight loss.

Perbedaan Kekuatan Geser Reparasi Gigi Tiruan Cekat dengan Resin Komposit Packable dan Flowable (Uji Laboratoris pada permukaan logam NiCr) Endang Wahyuningtyas; Suparyono Saleh; Sri Budi Barunawati
Majalah Kedokteran Gigi Indonesia Vol 19, No 2 (2012): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Latar Belakang. Resin komposit merupakan bahan pilihan untuk reparasi Gigi Tiruan Cekat porcelain fused to metal (PFM) Karena estetis baik dan manipulasi mudah. Tujuan penelitian ini adalah mengetahui perbedaan kekuatan geser perlekatan resin komposit pada permukaan logam Gigi Tiruan Cekat dengan menggunakan resin komposit jenis packable dan jenis flowable. Metode penelitian. Subjek penelitian berupa logam NiCr (Noritake, Japan) berbentuk silinder dengan diameter 10 mm dan tinggi 3 mm. Penelitian dibagi menjadi dua kelompok, masing-masing terdiri dari 10 subjek. Kelompok pertama reparasi dengan resin komposit packable (Z250™ 3M ESPE, USA) dan kelompok kedua reparasi dengan resin komposit flowable (Dyad flow, Kerr, USA). Permukaan subjek dikasari dengan wheel diamond bur, dietsa dengan asam fosfat 37 % (Scotchbond™, 3M ESPE, USA) kemudian dicuci dan dikeringkan, selanjutnya diaplikasikan silan (Rely X™ Ceramic Primer, 3M ESPE, USA) dan bonding (Adper™ Single Bond, 3M ESPE, USA). Permukaan kemudian dilapisi dengan resin komposit packable dan flowable, disinari selama 40 detik. Subjek penelitian direndam di dalam distilled water dan dimasukkan ke dalam incubator dengan suhu 37°C selama 7 hari. Uji kekuatan geser dilakukan dengan menggunakan Universal Testing Machine. Data dianalisis dengan uji t. hasil uji t menunjukkan terdapat perbedaan bermakna kekuatan geser reparasi gigi tiruan cekat pada permukaan logam (p<0,05). Kesimpulan penelitian ini adalah kekuatan geser reparasi pada permukaan logam dengan menggunakan resin komposit flowable yang mengandung bahan coupling agent lebih tinggi dibandingkan reparasi pada permukaan porselen dengan menggunakan resin komposit packable. Background. One of selected material by repairing the fixed partial denture was composite resin because of its good aesthetics and low manipulation. The aim. This research was aimed at identifying the differences of shear bond strength of composite resin on metal surface by using different composite resins, namely packable and flowable composite resins. Method. The research subjects were cylinders with 10 mm in diameter and 3 mm in height. The research subjects with metal material of NiCr (Noritke, Japan) involved two groups. The first group comprising 10 repair subjects with composite packable (Z250™, 3M ESPE, USA) and 10 repair subjects with flowable composite (Dyad flow, Kerr, USA). The surface of the subjects were roughned with wheel diamond bur and etched with 37% phosphate acid (Scotbond™, 3M ESPE, USA) were applied. The surface was then coated with packable and flowable composite resins and light-cured for 40 seconds. The research subjects were immersed in distilled water and put into the incubator at temperature of 37°C for 7 days. The shear bond strength test was conducted using the Universal Testing Machine. Data were analyzed using t-test. The result. The research result showed that there were differences of shear bond strength of repair between packable and flowable composite resins. The result of t-test indicated significant diffrences on metal surface (p<0,05). The conclusion of this research is that shear bond strength of repair with flowable composite which contain coupling agent has higher shear bond strength than that of packable composite resin.

PREPARATION FOR PALATOPLASTY SURGERY WITH AN ACRYLIC FEEDING OBTURATOR: A CASE REPORT Christine Anita Wardaningrum; Endang Wahyuningtyas; Titik Ismiyati; Sri Budi Barunawati
Dentino : Jurnal Kedokteran Gigi Vol 6, No 1 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i1.10650

ABSTRACTBackground: Clefts on the lips and palate are common cases to be found frequently in the field of dentistry. This gap connects the oral cavity and the nasal cavity so that it interferes with the nutritional intake in patients. The age and size of the oral cavity in patients become particular challenges for prosthetic rehabilitation. Another challenge lies at the impression procedure, because it is an essential part in the making of obturator. Objective: To provide information on the management of patient with cleft palate using feeding obturator from acrylic material. Case: A 13-months-old male patient arrived at Prof. Soedomo Gadjah Mada University Dental Hospital with his parents and was seeking for prosthetic therapy to cover the cleft on his palate because it had been muddling with food and beverage intake. Patient had undergone cheiloplasty surgery and further arranged for palatoplasty procedure. Intraoral examination exhibited a cleft extended from hard palate, soft palate, and uvula. Case management: Upper jaw impression was performed for individual tray fabrication, followed by impression procedure in an upright straight position until the patient bawled. The impression result was casted to construct an obturator using acrylic heat cure material. Fitting surface was covered with soft liner. Treatment result showed that the obturator was retentive, stable and able to assist the nutritional intake in patient. Conclusion: Acrylic feeding obturator can recover the condition in patient with cleft palate and improve nutritional intake as well as body weight to prepare patient for palatoplasty surgery.Keywords: Cleft Palate, Feeding Plate, Obturator

Effects of manufacturing methods of abalone gel as a desensitisation material on the closing of dentinal tubules Sri Budi Barunawati; Wayan Tunas Artama; Suparyono Saleh; Siti Sunarintyas; Yosi Bayu Murti
Dental Journal (Majalah Kedokteran Gigi) Vol. 53 No. 2 (2020): June 2020
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v53.i2.p99-106

Background: Abalone (Haliotis varia Linnaeus) shells possess a high arginine content and are expected to be an alternative desensitisation material that is both insoluble and able to properly close dentinal tubules. Different methods of manufacturing abalone gel affect the molecular weight, hydrophilic or hydrophobic properties and protein content of the lysis. Purpose: This study aimed to determine the effects of different manufacturing methods on the dentinal tubule closure of abalone desensitisation gel. Methods: This study involved the extraction of abalone shells followed by preparative and thin-layer chromatography. The drying of the samples was carried out by the precipitation, drying, and addition methods. The research was divided into eight treatment groups, each consisting of three samples (F1, F2, F3). Each sample was applied to two study subjects’ post-extracted third molars, which were cut into disc shapes and subsequently etched with 6% citric acid. The percentage of dentinal tubule occlusion was calculated by Image J (NIH, USA) software. Data were analysed using three-way ANOVA. Results: The results showed that there were significant differences (p < 0.05) both in terms of the effects of the samples with deposition and addition on the occlusion of the dentinal tubules and in terms of the interactions between the samples with drying and addition. There was no significant difference (p > 0.05) in terms of the interactions of the samples’ three manufacturing methods. Conclusion: The manufacture of abalone gel as a desensitisation material requires a minimum of two interactions between the sample-making method and the addition, deposition, and drying methods. The best method was deposition.

Prosthetic Rehabilitation with Artificial Palpebra of an Ocular Defect with Contracted Socket Chrisdina Puspita Sari; Sri Budi Barunawati; Intan Ruspita; Endang Wahyuningtyas
Indonesian Journal of Prosthodontic Vol 5 No 1 (2024): June 2024
Publisher : Indonesia Prosthodontic Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46934/ijp.v5i1.225

Eyes are one of the most important organs of the body which are set inside the protective orbital bone. Loss of an eye can be caused by congenital defects, trauma, or tumors. The removal of the eyeball (enucleation) may cause a massive impact on the self-image, confidence, and personality of an individual. Acceptable cosmetic results usually can be obtained with an ocular prosthesis which is an artificial maxillofacial prosthesis to replace the lost eye. This paper describes the prosthetic rehabilitation of a 71-year-old male patient with a left ocular defect accompanied by a contracted socket caused by the poorly fitting old prosthesis which results in subpar cosmesis. The soft tissue around the eye is normal, but the palpebral conjunctiva forms an abnormal adhesion to the eye socket. An impression was made using a custom tray and alginate in the defect area, followed by filling the impression with gypsum type 3 to get a working model, from which a wax model was made and adjusted to the patient. Sclera and ocular acrylic prostheses were made on the basis of an adjusted wax model. The prosthesis was then polished and colored according to the contralateral eye, followed by the manufacturing of an artificial palpebra made of silicone for a more aesthetic result. The custom-made eye prosthesis with silicone palpebra is an artistic solution for the treatment of ocular defects with contracted sockets. This technique was able to improve the patient’s psychological and emotional status.

The relationship between bizygomatic width and mesiodistal width of six maxillary anterior teeth in a Javanese population Indrastuti, Murti; Barunawati, Sri Budi; Hasudungan, Dian Samuel
Majalah Kedokteran Gigi Indonesia Vol 8, No 3 (2022): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.79146

The selection of the mesiodistal width of maxillary anterior teeth is the key to success in prosthodontic treatment. Determining anterior teeth width when there is no existing pre-extraction record can be done through bizygomatic width measurement. The purpose of this study was to look at the relationship between bizygomatic width and mesiodistal width of six maxillary anterior teeth in the Javanese population. This study used a cross-sectional design and was conducted on 32 Javanese subjects who met the following criteria: being 18-25 years of age, having Angle class 1 malocclusion, having complete maxillary anterior permanent teeth, not using dentures, currently not undergoing any orthodontic treatment, and having no abnormalities found in the facial area. The bizygomatic width was measured with a digital caliper with an accuracy of 0.01 mm from the marked right and left bizygomatic points. The mesiodistal width of the six maxillary anterior teeth was measured using dental floss looped from the distal left canine to the right canine. Dental floss marked with a pen was stretched horizontally on paper and then measured with a digital caliper with an accuracy of 0.01 mm. The measurement was done in triplicate. Data were analyzed using Pearson’s correlation and the simple linear regression test. This study’s analysis revealed a strong and positive correlation (r = 0.538 and p < 0.05) between the bizygomatic width and mesiodistal width of the maxillary anterior teeth. The result of the simple linear regression test showed a significant result (p < 0.05). It can be concluded that there was a relationship between the bizygomatic width and the mesiodistal width of the six maxillary anterior teeth in the Javanese population. The mesiodistal width of the six maxillary anterior teeth in the Javanese population can be measured by multiplying the bizygomatic width by the multiplier factor, i.e., 0.427.

Prosthetic rehabilitation of nasomaxillary defect with TAD retained surgical obturator followed by hollow bulb definitive obturator and immediate lower denture Wijaya, Agustinus Kenny; Barunawati, Sri Budi; Fransiscus Wihan Pradana; Suparyono Saleh
Indonesian Journal of Prosthodontic Vol 5 No 2 (2024): December 2024
Publisher : Indonesia Prosthodontic Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46934/ijp.v5i2.217

The integrity and functionality of the oral cavity may be compromised by nasomaxillary defects, such as speech, mastication, deglutition, and esthetics. Early prosthodontic rehabilitation can improve quality of life and lessen the psychological harm caused by the surgical treatment. A 70-years-old female patient was referred to the Department of Prosthodontics RSUP Dr. Sardjito Yogyakarta with chief complaints of nasal obstruction, and right-side swelling of the face. Extraoral examination revealed facial asymmetry due to swelling of the right buccal region. On intraoral examination, showing a large, firm mass extending from right palate to the midline. A multidisciplinary approach with ENT is required for the surgical treatment. A surgical obturator was made before the surgery, and immediately inserted after with TAD as a retention device. Three months later, a hollow bulb obturator and immediate lower denture was fabricated so the functional capabilities of speech, mastication, deglutition, and esthetics can be restored. Keywords: surgical obturator, maxillofacial prostheses, definitive obturator, hollow bulb

Comparison of bombyx mori l. Fibroin coatings on the mechanical properties of collagen membranes Fathurrahman, Helmi; Sunarintyas, Siti; Barunawati, Sri Budi; Nuryono, Nuryono
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.146-151

Background: Most of dental implants are accompanied by guided bone regeneration (GBR) procedures. Collagen membrane which is the most widely used type of membrane in the GBR procedure has the disadvantage of not having antimicrobial properties, old bone formation, easy to tear, and soft so that it does not maintain the dimensions of the augmentation area. Bombyx mori L. fibroin has good mechanical properties and has the potential to be used as a pharmaceutical material that is biocompatible, antimicrobial, and stimulates bone growth. The aim of this study was to analyze the effect of Bombyx mori L. fibroin coating on the mechanical properties of collagen membranes. Method: The samples consisted of the control group (group A) and the treatment group, the collagen membrane was coated using the Bombyx mori L dip-coating fibroin technique, namely group B (1 time coating process), group C (2 times coating process) and group D (3 times the coating process). Statistical analysis used one-way ANOVA (p

Comparison of bombyx mori l. Fibroin coatings on the mechanical properties of collagen membranes Fathurrahman, Helmi; Sunarintyas, Siti; Barunawati, Sri Budi; Nuryono, Nuryono
Odonto : Dental Journal Vol 10, No 1 (2023): July 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.1.146-151

Background: Most of dental implants are accompanied by guided bone regeneration (GBR) procedures. Collagen membrane which is the most widely used type of membrane in the GBR procedure has the disadvantage of not having antimicrobial properties, old bone formation, easy to tear, and soft so that it does not maintain the dimensions of the augmentation area. Bombyx mori L. fibroin has good mechanical properties and has the potential to be used as a pharmaceutical material that is biocompatible, antimicrobial, and stimulates bone growth. The aim of this study was to analyze the effect of Bombyx mori L. fibroin coating on the mechanical properties of collagen membranes. Method: The samples consisted of the control group (group A) and the treatment group, the collagen membrane was coated using the Bombyx mori L dip-coating fibroin technique, namely group B (1 time coating process), group C (2 times coating process) and group D (3 times the coating process). Statistical analysis used one-way ANOVA (p

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