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Biocelebes
Published by Universitas Tadulako
ISSN : -     EISSN : 25805991     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 10 Documents
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Search results for , issue "Vol. 12 No. 2 (2018)" : 10 Documents clear
Autekologi Impatiens mamasensis Utami & Wiriad. di Kawasan Resort Tongoa Taman Nasional Lore Lindu Sulawesi Tengah Dian Moh Fauzan; Ramadhanil Pitopang; Samsurizal Suleman
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

A research entitled “Autecological study of Impatiens mamasensis Utami & Wiriad. In the Tongoa resort of Lore Lindu National Park Central Sulawesi has been carried out from April to May 2016. The research objectives was to describe the environmental factor of habitat of I. mamasensis Utami & Wiriad including abiotic and biotic factor. The abiotic factors which was observed such temperature and humidity were measured by Termohygrometer, light intensity by lux meter, and  soil pH and soil humidity were calculated by Soil tester. Geographical position measured by Global Positioning System/ GPS). Meanwhile, rainfall data obtained from the Meteorological and Geophysics Palu. Concentrations of Nitrogen, of habitat was analyzed by the Kjehdahl method whereas Phosphorus, and Potassium was analyzed by extracting 25% HCL. Vegetation was analyzed by using double plot method with plot size 2 by 2 m in size. The results showed that  I. mamasensis grew well at an altitude of 1500 to 1700 m asl with daily temperatures ranged from 17.3 to 23.8 ° C, relative humidity varied from 68.5 to 83.7% soil pH  from 5,8 to 7 and soil humidity ranges from 50 to 80%.  Rainfall was 448 mm and 205 during  April to May. I. mamasensis grow in low concentration N meanwhile concentration P and K soil with moderate concentration. The vegetation is dominated by Elatostema strigosum (Bl.) Hassk (IVI 14,81%.), Desmodium repandum (Vahl.) DC. (IVI 9,71%), Crypsinus montanus Sledge sp (IVI 9,56%), Geophila repens (L.) I.M. Johnst. (INP 9,29%). I noted a number species of  insects ie, Tetrigidae, Tachinidae,  Aulacophora sp, Chrysosoma sp, and Episyrpus sp. The results of this study are expected to provide useful information for the benefit of ex-situ conservation acclimation and cultivation research Impatiens mamasensis Utami & Wiriad. in order to prevent this endemic species from extinction.Key words:  Autecology, Impatiens mamasensis Utami & Wiriad., Lore Lindu National Park (LLNP)..
EFEKTIVITAS ANTIINFLAMASI EKSTRAK ETANOL DAUN SUMAMBU (Hyptis capitata Jacq.) PADA TIKUS PUTIH JANTAN (Rattus norvegicus L.) YANG DIINDUKSI DENGAN KARAGENAN Mia Audina; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

One of the medical plants that can be used as an anti-inflamation treatment is sumambu leaf (Hyptis capitata Jacq.). Most of people in binangga village, Subdistric of central Parigi, Regency of Parigi Moutong, have been used this sumambu leaf for deducting inflammation, swelling, and painful. The aimed of this research is to know the effectiveness of the anti-inflamation of Sumambu leaf (Hyptis capitata Jacq.) towards white male mouse (Rattus novergicus L.) which has been given carrageenan induction. The extract was made by using a maseration method using 96% ethanol liquid. The test of the effectiveness of anti-inflamation was divided into your treatments which each treatment was consist of five mice which have been given negative control  0.5% CMC-Na suspension ;100 mg/KgBW, 200 mg/KgBW, 300 mg/KgBW of sumambu leaf extract. After 60 minutes, 0.1 ml carregeenan has been injected using subplantar method at the paw of the mice. The inflamation volume measured using  pletysmometer for 6 hours.  The data collection was statistically analyzed by ANOVA (Analysis of variance) one direction and continued to Duncan test. The results of this research  showed that the three doses of sumambu extract which are ES 100 mg/KgBW, 200 mg/KgBW, 300 mg/KgBWhave the effect of anti-inflamation. Moreover, it can be said that the result of this research showed that the dose that has a lower inflamation percent was ES 300 mg/KgBW. Keywords: Anti-inflammation, Hyptis capitata Jacq., Carrageenan, Inflamed Paw Volume.
Efek Akar Garcinia rostrata Hassk.ex Hook.f Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan (Mus musculus) Yang Diinduksi Aloksan Devi Saputri Bahman; Yuliet Yuliet; Ihwan Ihwan
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Garcinia rostrata Hassk.ex Hook.f is a plant that empirically used for lowering blood glucose levels. This study aims to determine antidiabetic activity of the n-hexane, ethyl acetate and ethanol-water fractions of its root Garcinia rostrata Hassk.ex Hook.f extract as well as to compare their effects to Glibenclamide as the positive control in alloxan-induced mice. The mice divided into five groups. The first group was given Na.CMC 0.5% (as negative control) and the second one was given Glibenclamide 0.65 mg/kgBW (as positive control), while the other three was given of n-hexane, ethyl acetate and ethanol-water fractions, each at a dose of 130 mg/kgBW. Before given the treatment, the mice was induced by alloxan at a dose of 150 mg/kgBW, by intraperitonial route. At fourth day after induced, the mice its raising blood glucose level >200 mg/dL was given a treatment 25 days by oral route. The level of mice blood glucose was measured 4 times at day 4, 11, 18, and 25, the blood is taken from lateralis vein tail of mice.  Data obtained were statistically analyzed by ANOVA and continued by Duncan test. Based on those results, it can be concluded that the ethanol-water fraction at doses of 130 mg/kgBW has antidiabetic activity in hyperglycemic that is comparable to Glibenclamide. Whereas, ethyl acetate fraction, at dose of 130 mg/kgBW has greater activity in lowering blood glucose level than glibenclamide. Keywords: Garcinia rostrata Hassk.ex Hook.f,  Fraction,  n-hexane,  Ethyl acetate, Ethanol-water, Alloxan. 
FORMULASI SUBSTRAT DASAR KOTORAN AYAM DAN LIMBAH CAIR TEMPE DENGAN INOKULUM RUMEN SAPI UNTUK STUDI AWAL PRODUKSI BIOGAS Nurhayati N Mangopo; Umrah Umrah; Kasman Kasman
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Production of biogas using the basic substrate of tempeh’s liquid waste and chicken’s waste with the bacteria inoculum of cow’s rumen has been experimentally conducted. This study aims to produce biogas using the substrate with the bacteria inoculum of cow’s rumen and know the optimal mixture ratio of the basic substrate and the inoculum. In this study, the amounts of basic substrate applied were 500 g chicken’s waste and 500 mL liquid waste of tempeh. The study was designed using Complete Random Design comprising 6 treatments with 3 times repetitions, respectively. The treatments tested were P0 (100% basic substrate), P1 (80% basic substrate + 20% inoculum), P2 (60% basic substrate + 40% inoculum), P3 (40% basic substrate + 60% inoculum), P4 (20% basic substrate + 80% inoculum), P5 (100% inoculum). These treatments were fermented with anaerob bacteria for 10 days in the fermentor 1000 mL mounted with the biogas collecting balloon. Variables observed in this study were the measurements of biogas volume, culture medium pH, and biogas burning temperature, and the testing of biogas flame colour. As result of observation, unlike P5, the examined treatments of P0, P1, P2, P3 and P4 could able to produce biogas with the respectively produced volume of 53,6 cm3, 56,7 cm3, 30,0 cm3, 90,0 cm3 and 73,3 cm3. While, the measured values of culture medium pH and biogas burning temperature were 6,23-6,63 and 152oC with the flame of red colour. This study shows that the optimal mixture ratio of the basic substrate and the inoculum for producing biogas is 2:3, and the produced biogas is potential to be used as an alternative energy. Keywords: Biogas, Chicken’s waste, Liquid waste of tempeh, Cow’s rumen
PENGARUH PEMBERIAN TOPIKAL EKSTRAK ETANOL DAUN PECUT KUDA (Stachytarpheta jamaicensis (L.) Vahl) TERHADAP PENYEMBUHAN LUKA BAKAR DERAJAT II PADA PUNGGUNG KELINCI (Oryctolagus cuniculus) Muhammad Anggriawan; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Pecut kuda (Stachytarpheta jamaicensis (L.) Vahl) is one of plant that has the potentials to heal various diseases, one of them as an alternative in the cure of burns. Pecut kuda leaf has been used hereditary by some people at Layana Wintu, Mantikulore subdistrict, Donggala regency for cure of burns. This research is conducted to find out the effect of topical administration of ethanol extract of pecut kuda leaf towards healing burns second degree on the rabbit’s back (Oryctolagus cuniculus) by using many extract concentration variation which are 1%, 3% and 5% with gel basic as the carrier. Testing was conducted on 5 rabbits, each rabbit was induced on his back by 5 burns for five treatments with burns diameter is 2 cm . Burns that has been induced was given each treatments which are negative control (gel basic without extract), ethanol extract of pecut kuda leaf in basic gel with each concentration 1%, 3% and 5%. Positive control used bioplacenton gel. Later, the burns diameter was measured on certain day during 28 days. Data that are achieved was  statistically analyzed  with  one way anova method and is continued by testing post hoc duncan with 95% confidance level. The reasult of this research shows every extract ethanol concentration variation of pecut kuda leaf have activity to heal burns. Extract ethanol concentration of pecut kuda leaf which is efective in healing burns is 3% concentration.Keywords: Gel, (Stachytarpheta jamaicensis (L.) Vahl), Burns, Oryctolagus cuniculus
Uji Aktivitas Antibakteri Formula Pasta Gigi Ekstrak Batang Karui (Harrisonia Perforata Merr.) Terhadap Bakteri Streptococcus Mutans Wardatul Nurjannah; Yusriadi Yusriadi; Arsa Wahyu Nugrahani
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Karui (Harrisonia perforata Merr.) is one of the many species that live in tropical regions such as Central Sulawesi (Palu) and has antibacterial activity. This research aims to find the variation effect of karui stem extract (Harrisonia perforata Merr.) in toothpaste towards stability of physical quality and antibacterial activity bacteria Streptococcus mutans. The concentrations of karui stem extract 1%, 2%, and 3% in toothpaste tested for antibacterial activity using pitting method which further tested for statistical analysis "One Way Anova" using SPSS 16.0 software. The results of the evaluation of toothpaste Karui stem extract F1 (1%), F2 (2%), and F3 (3%) showed that the test of the organoleptic and homogenity has no significant difference during the storage time (28 days) but the test at pH, viscosity, and the scatter has significant difference. The Formula of karui stem ekstract with 3 concentrations F1 (1%), F2 (2%), and F3 (3%) forming an average diameter of antibacterial inhibitory 6.65 mm; 7.14 mm; and 9.85 mm. The results of statistical analyzed from toothpaste test diameter inhibitory zone showed an unsignificant difference. Keywords: toothpaste, extract, karui (Harrisonia perforata Merr.), Streptococcus mutans
MONITORING KETAHANAN HIDUP SEMUT HITAM Dolichoderus thoracicus Smith PADA SARANG BUATAN DI TANAMAN KAKAO Murnawati Murnawati; Annawaty Annawaty; Umrah Umrah
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The Black Ant, Dolichoderus thoracicus Smith have long been recognized as a potential biological control agent in Cocoa plantation. The aims of this study were to determine how long D. thoracicus can survive on artificial nests in cacao plantation and to identify the ant competitor of D. thoracicus. The research conducted in three cocoa plantation i.e. Desa Labuan Panimba, Desa Langaleso, and Desa Pantoloan in Donggala District, Sulawesi Tengah. Artificial nest of D. thoracicus made from bamboo and palm leaves smeared with palm sugar solution and then hung in cocoa trees. The black ant, D. thoracicus can be survived for thirty days in the cacao plantation in Desa Labuan but only one day in Desa Langaleso. The weaver ant (Oecophylla smaragdina) and Philidris sp. were identified as the competitors of D. thoracicus ants inside the artificial nests. Keywords: Dolichoderus thoracicus, artificial nests and ant competitors.
AKTIVITAS EKSTRAK KULIT BATANG LANGSAT (Lansium parasiticum (Osbeck) Sahni & Bennet) TERHADAP KELARUTAN KALSIUM BATU GINJAL SECARA in vitro Nur Ramadhani; Yuliet Yuliet; Khildah Khaerati
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

The kidney stone is a stone which is found in the kidney or other parts of urinary tract. The most frequently type of stone encountered are composed of calcium crystals. As much as 60% content of kidney stones consist of calcium oxalate, phosphate, carbonate, uric acid, magnesium, and ammonium. Langsat (Lansium parasiticum (Osbeck) Sahni & Bennet) is a plant that may dissolve kidney stones and smooth urination, particularly its bark. This study aims to determine the activity as well as the greatest concentration of Langsat bark ethanol extract in dissolving dissolves calcium of kidney stones conducted in vitro. The ethanol extract was obtained by maceration method using 96% ethanol followed by phytochemical screening test and thin layer chromatograpy (TLC) profiling of the extract. Chromatogram result shows that the extract contains flavonoids. The research methodology conducted was the kidney stone powder dissolution in the ethanol extract of Langsat bark after being destructed with concentrations of respectively 0.5%, 1% and 1.5% then incubated in 37ºC temperature for 3 hours. The solubility of calcium was assayedby measuring the calcium (Ca) levelsat the beginning and at after being incubated with the kidney stone.The positive control used was Calcusol® and the negative onewas aquadest. The dissolved calcium levels were measured by using Atomic Absorption Spectrophotometer at  λ = 422.7 nm. The research results show that Langsat Bark ethanol extract has the activity in dissolving the calcium of kidney stones in vitro of which concentration with greatestactivity of calcium dissolution is 1.5% w/v. Keywords: Kidney Stone, Langsat, Lansium parasiticum, Calcium.
Aktivitas Antiinflamasi Gel Ekstrak Rumput Mutiara (Ordelandia corymbosa L.) Pada Tikus (Rattus norvegicus L.) Yang Diinduksikan Karagenan Ince Agus Nurcholis; Yusriadi Yusriadi; Evi Sulastri
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Pearl grass is usually used in traditional medicine for the treatment of inflammation. This research aims to determine the anti inflammatory activity of pearl grass extract gel in rats induced by carrageenan and to evaluate the physical stability of its formulation during the storage of 14 days. The gel physical stability tests consist of organoleptic test, homogeneity, pH viscosity and dispersive power. Anti inflammatory activity test were divided into 5 treatment groups. Group 1 was given a gel without active ingredients as a negative control, group 2 were given diclofenac sodium gel as a positive control and group 3, 4 and 5 were given pearl grass extract gel at concentration of 5%, 10% and 15%. The measurements of inflammation volume were performed for 6 hours with intervals of 60 minutes. The results showed that all formulas have good physical stability during storage for homogeneity testing but having instability on pH, viscosity and dispersive power. The activity test showed all formulas have anti-inflammation against carrageenan induced rat. Gel with a concentration of 10% (F2) which more effective to reduce inflammation than the other formulas was selected as the most optimum formula. Key words : Gel, extract gel, pearl grass, Ordelandia  corymbosa L., antiinflammatory
STUDI TUMBUHAN BERKHASIAT OBAT PADA SUKU KAILI DA’A KECAMATAN KINOVARO KABUPATEN SIGI SULAWESI TENGAH Sri Rizqi Muthmainnah; Nurlina Ibrahim; Ririen Hardani
Biocelebes Vol. 12 No. 2 (2018)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

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Abstract

Study of medicinal plants on Kaili Da’a tribe at Kinovaro district, Sigi Regency in Central Sulawesi has been done to preserve the knowledge and use of herbs for traditional medicine. This study aims to determine the type of plants used as medicine, the type of disease being treated as well as how to use these plants by Da'a tribe. This research is a descriptive study using qualitative methods with a sampling technique that snowball sampling through interviews and direct observation of 7 respondents of Kaili Da'a tribe. Based on the interview, known as much as 31 families of medicinal plants. The results of identification of the plants used as medicines known 65 species, 6 species which have been unidentified its species (2 species unidentified families). The most widely used plant is from Euphorbiaceae family (6 species). Parts of plants used include leaves, stems, fruits, roots, herbs, seeds, sap and flowers. The leaves are part of plants that have the highest usage percentage (77%). As for how to use of medicinal plants is done by mouth, chewed, spilled, smeared, taped, wrapped, dyed and used in massage. How to use the most that drunk as much as 70%.

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