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Biology, Medicine, & Natural Product Chemistry

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Published by Universitas Islam Negeri Sunan Kalijaga Yogyakarta

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Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

BIOLOGY, MEDICINE, & NATURAL PRODUCT CHEMISTRY, this journal is published to attract and disseminate innovative and expert findings in the fields of plant, animal, and microorganism secondary metabolite, and also the effect of natural product on biological system as a reference source for researchers in these fields, and with the aim to set international standards in their methodology.

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Articles 6 Documents

Search results for , issue "Vol 7, No 1 (2018)" : 6 Documents clear

Leukocytes Description of Mudskipper (Periophthalmodon schlosseri) of Barito River Estuary, Desa Tanipah, Kalimantan Selatan Heri Budi Santoso; Hidayaturrahmah Hidayaturrahmah; Muhamat Muhamat
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Leukocytes have an important role in driving away infections from pathogen microorganism by phagocytosis together with macrophages. The aim of this research was to analyze the leukocytes’ differentiation (eosinophil, basophil, neutrophil, lymphocytes, and monocytes) of mudskipper‘s blood (Periophthalmodon schlosseri). Samples were taken from desa Tanipah Kalimantan Selatan. The sampling locations were decided by purposive or taking the data intentionally according to the consideration of mudskipper‘s location which is not homogenous. The data collection were conducted using Line Transect which means the sample was taken according to how many encounters can be taken. There were 15 mudskippers taken from Desa Tanipah used in this research. The parameter observed wasthe differentiation of leukocytes which consists of the percentages of monocytes, lymphocytes, eosinophil, basophil, and heterophil. The result showed that the percentage of lymphocytes in mudskippers is 62+4,1% and monocytes 24,7+0,8%, and the neutrophil is 0,5+0,1% and eosinophil 0,6+0,1% and no basophils were found. According to the result, it can be concluded that lymphocytesis the most dominant one amongst others.

Stability of T-DNA Integration in Phalaenopsis “Sogo Vivien” Transgenic Orchid Carrying 35S::Gal4::AtRKD4::GR Endang Semiarti; Exsyupransia Mursyanti; Ahmad Suyoko; Faiza Senja Widya Perdana; Catharina Tri Widyastuti; Aditya Nur Subchan
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Orchid is an elegant ornamental plant and favoured by the society. Phalaenopsis "Sogo vivien" is a mini-sized orchid with an interesting white-striped purple petals. This study was aimed to analyze the stability of the integration of embryonic gene carrier T-DNA from Arabidobsis AtRKD4 into the P. "Sogo vivien" genome produced in 2016. The study was conducted in 3 stages: 1) Transgenic plant phenotype analysis (1 year old); 2) Examination of T-DNA integration in orchid genotypes using PCR. 3) Analysis of transgenic plant leaf explants’ ability to produce somatic embryo in vitro. In vitro cultures were performed on the base medium of New Phalaenopsis (NP), plus various concentrations of TDZ (0, 1, 2 mg.L-1) and IBA (0, 1, 2 mg.L-1) or without TDZ and IBA as controls. The transgenic Phalaenopsis ‘Sogo vivien’ were transferred to pot mediums via ex vitro with two treatments: the first leaves were cut as explants for in vitro culture, and the plants were transferred to the mixture of fern medium with shavings of bark. The integration of T-DNA in the genome was detected by DNA genome amplification from the second leaves using the AtRKD4 gene primers and the POH1 gene. The results showed that the highest number of somatic embryo (SE) propagules or protocorm like bodies (PLBs) amounted to 27 were derived from transgenic plant # 2 cultured on NP + 2 mg.L-1 TDZ +1 mg.L-1 IBA medium. The presence of AtRKD4 transgenes were detected with the amplification of 380 bp of the RKD4 gene from the genome of transgenic plant # 2 by using PCR. There were 2 out of 15 plants that positively carry the AtRKD4 gene and produce SE. Thus, the stability of the AtRKD4 carrier T-DNA integration in the genomes of transgenic plants was 13.3%.

Comparison of detergent and CTAB method for isolation of DNA from Salak ( Salacca zalacca (Gaert.) Voss. Ã¢â‚¬ËœPondohÃ¢â‚¬â„¢) Arfa, Namira Nur; Daryono, Budi Setiadi; Reflinur, Reflinur
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

This study conducted in Laboratory of Molecular Biology, Balai Besar Bioteknologi dan Sumberdaya Genetik Pertanian (BB- Biogen) Bogor. The aims of this study are to determine and comparing the quantity,Ã‚Â quality and the efficiency of DNA isolation result using detergent method and CTAB method.Ã‚Â The parameters observed in this study are the value of DNA concentration, purity, and visualization result using gel electrophoresis. The samples are the leaves of Salak Ã¢â‚¬ËœPondohÃ¢â‚¬â„¢ (Salacca zalacca (Gaert.) Voss.). Detergent method is a method which was developed by Faculty of Biology UGM, it has simple method and relatively affordable cost. Meanwhile, CTAB method is one of the commonly used methods of DNA isolation protocol with relatively expensive cost.Ã‚Â Detergent method used detergent in the cell wall separation and protein removal in the sample. The CTAB method used Cetyltrimethyl ammonium bromide (CTAB) for cell membrane separation in the sample. The research methods included DNA isolation with detergent and CTAB methods, PCR analysis and electrophoresis. Data analysis was done quantitatively Ã‚Â using spectrophotometric method and qualitative used electrophoresis method. The result of the study Ã‚Â showed that DNA isolation usingÃ‚Â CTAB method showed higher purity compared with detergent method with the purity values ranging from 1,3- 1,4 . Meanwhile, the concentration of DNA in the detergent method was higher than that of CTAB with the highest concentration of 1730 Ã‚Âµg/ml. There is no difference between theÃ‚Â quality of genomic DNA isolated by CTAB and detergent methods.

Physiological Response of 'Segreng' Rice Plant (Oryza sativa L.) to Biogas Sludge at Wukirsari Village, Cangkringan, Sleman Dwi Umi Siswanti; Nindy Senissia Asri; Mifta Arlinda; Arianda Poetri Shofia Rochman; Akrima Syahidah
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Wukirsari Village, Cangkringan District is belong to Merapi Mountain’s slopes which located between the Gendol River and Yellow River. Nowadays, we faced the problem of anorganic fertilizer overused such as Urea, ZA, TSP/SP-36 and KCl in agriculture land. The effort to return the soil organic compound can be done by added some organic compounds or microbial bio -organic fertilizer. Sludge is fermented biodigester yield and it has lost its gas. The aim of this research was to understand the physiological response and optimum dose of biogas as planting medium to ‘Segreng’ Rice planted in the rice field of Wukirsari Village, Cangkringan District, Sleman Regency. This research was done on greenhouse scale and rice field scale. The treatment given on 0; 1; 1,5; 2 and 2,5 liters per 100 m2 of rice field areas, and given on 0; 4; 8; 12; and 24 ml per 5 kg soil on polybags. Data were taken in three repetitions. The vegetative growth parameters included plant height, number of leaves, number of seedlings and chlorophyll content, while generative growth parameters measured included NRA levels, dried biomass including crown/stem, roots, filled grains, empty grains, and total weight and number of filled grains, empty rains, and the number of panicles. The result were tested with ONE WAY ANOVA (Analysis of Variance) with SPSS version 19 for Windows and followed by Duncan's Multiple Range Test with 95% significance level (α = 0.05). Generally, the result showed that biogas sludge can increase the vegetative and generative growth of rice plant ‘Segreng’ on polybag scale and rice field scale. The rice plant on polybag with 4 ml biogas sludge was significantly different on the vegetative growth and chlorophyll content, while the rice plant on polybag with 8 ml biogas sludge was significantly different on the generative growth and NRA levels.

Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening Mohamad Amin; Kurniawan Setia Putra; Ihya Fakhrurizal Amin; Nanda Earlia; Dina Maulina; Betty Lukiati; Umie Lestari
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Inflammation is a tissue injury that occurs due to physical trauma or microbiological substances that involve the activities of many cell types. Inflammation can be prevented using the natural medicines from Allium cepa L. Quercetin is one of the bioactive compounds found in Allium cepa L and has been reported to have anti-inflammatory activity. The natural medicines have been used to minimize non-steroidal anti-inflammatory drugs. This study aims to investigated the modeling structures and the protein receptor from quecertin in inflammation mechanism and their optimization of the effectiveness in the human body. The bioinformatics tools used in this study are the database of quercetin compounds, Pubchem and Swis Target Prediction protein prediction databases, PyRx 0.8 molecular docking software, ligand docking, and binding site analysis with PyMOL and LigPlus software. The results from in silico show that quercetin compounds can interact with Muscleblind-like protein 1 target protein with a Binding Affinity minus value which is not much different from the dexamethasone compound. Dexamethason is a standart because it is a corticosteroid drug that can be used as an anti-inflammatory to reduce inflammation, allergic reactions, arthritis and other inflammatory diseases.

Cover, Editorial Board, Guidance for Authors, & Table of Contents Riyanto Riyanto
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

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