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Biomedika
Published by Universitas Muhammadiyah Surakarta
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Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
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Articles 399 Documents
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PERUBAHAN EKSPRESI HEAT SHOCK PROTEIN 70 AKIBAT PAPARAN MEDAN ELEKTROMAGNETIK EXTREMELY LOW FREQUENCY PADA MAKROFAG PERITONEUM MENCIT YANG DIINFEKSI Toxoplasma gondii Taufiqurochman, Mochammad Arief
Biomedika Vol 7, No 2 (2015): Biomedika Agustus 2015
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v7i2.1900

Abstract

Penelitian dilakukan untuk mengetahui efek paparan medan elektromagnetik ELF sebesar 100 ?T 8 jam/ hari selama 2 dan 4 minggu terhadap ekspresi HSP 70 makrofag peritoneum mencit yang diinfeksi dengan Toxoplasma gondii. Jenis penelitian ini adalah Eksperimen Biomedik menggunakan rancangan randomized separate posttest control group designdengan hewan coba mencit strain Balb/c, melalui pengamatan ekspresi HSP 70 , terdiri dari 3 kelompok kontrol dan 4 kelompok perlakuan, tiap kelompok terdiri dari 4 hewan coba. Pengamatan jaringan menggunakan metode imunohistokimia indirek, hasilnya dianalisis menggunakan uji statistik Independent t-test antar kelompok setelah dilakukan uji homogenitas dan normalitas data penelitian ( ?=0.05). Hasil penelitian menunjukkan bahwa akibat paparanME ELF dengan itensitas 100 ?T selama 2 minggu belum mampu melemahkan atau memutus rantai DNA gen HSP 70 promotor region tetapi menimbulkan stres seluler yang berakibat teraktifasinya HSF 1 melalui konversi menjadi trimer yang akan meregulasi secara cepat sintesis HSP 70 . Paparan medan elektromegnetik ME ELF selama 4 minggu dapat melemahkan bahkan memutus rantai DNA hsp 70 promotor region, sehingga sintesis HSP akan terhambat secara signifikan (p<0.05). Terdapat peningkatan secara signifikan ekspresi HSP 70 makrofag peritoneum mencit yang terpapar ME ELF dengan itensitas 100 ?T selama 2 minggu pada kelompok yang terinfeksi toxoplasma gondii dan terjadi penurunan secara signifikan ekspresi HSP 70 pada kelompok terpapar ME ELF selama4 minggu pada kelompok yang terinfeksi Toxoplasma gondi dibandingkan dengan konrol.Kata Kunci: Medan Electromagnetik ELF, HSP 70, Makrofag, Toxoplasma gondii.
PEMISAHAN SENYAWA-SENYAWA YANG BERSIFAT SITOTOKSIK TERHADAP SEL MURIN LEUKEMIA P388 DARI EKSTRAK METANOL KULIT BATANG DIPTEROCARPUS CONFERTUS SLOOT (DIPTEROCARPACEAE) Muhtadi, .; Indrayudha, Peni
Biomedika Vol 5, No 1 (2013): Biomedika Februari 2013
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v5i1.269

Abstract

Six compounds, KP-1 up to KP-6 were isolated from the methanol extract of the tree bark of Dipterocarpus confertus Sloot (Dipterocarpaceae). The cytotoxic activities of these compounds were evaluated against murine leukemia P388 cells. Result of these examination indicated that KP-2 compound and KP-1 were very active with each IC 50 value of these compounds were 2.25 and 5.1 ?g/mL, respectively. While the others were not active against murine leukemia P388 cells.Keywords: Dipterocarpaceae, Dipterocarpus confertus Sloot, isolated compounds, cytotoxic, and leukemia P-388 cell
UJI DAYA ANTIMIKROBA EKSTRAK ETANOL DAUN SIRIH MERAH (Piper Crocatum Ruiz & Pav.) TERHADAP PERTUMBUHAN Staphylococcus aureus ATCC 6538, Eschericia coli ATCC 11229 DAN Candida albicans ATCC 10231 SECARA IN VITRO Candrasari, Anika; Romas, M. Amin; Astuti, Ovi Rizky
Biomedika Vol 4, No 1 (2012): Biomedika Februari 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v4i1.258

Abstract

Red betel leaf contains flavonoids, alkaloids and essential oils that are suspected able to inhibit the growth of microbes. This study aims to analyze antimicrobial activity of ethanol extract of red betel leaf agains to Staphylococcus aureus, Eschericia coli and Candida albicans. Subjects were red betel leaf ethanol extract at concentration of 2.5%, 5%, 10%, 20%, 40%, 80% and 100%. As a positive control used amoxycilin, chloramphenicol and ketoconazole. The study showed that concentration 10 % - 100 % inhibit the growth of Staphylococcus aureus. In Escherichia coli all the data obtained, having an average which is not much different from the mean of the negative control. While on Candida albicans average diameter of inhibition zone of extract concentration 40% p = 0.197 concluded that statistically has significant antifungal power compared with ketokonazole.Keywords: Ethanol extract, Piper Ruiz & Pav crocatum, Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albican
PENGARUH PEMBERIAN AIR SEDUHAN BERAS YANG DIFERMENTASI OLEH MONASCUS PURPUREUS (ANGKAK) TERHADAP PENURUNAN KADAR KOLESTEROL SERUM PADA TIKUS PUTIH Wicaksono, Adhimass; Sintowati, Retno; Fitriyah, Sa?idatul
Biomedika Vol 6, No 1 (2014): Biomedika Februari 2014
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v6i1.284

Abstract

The using of plant-based medicine is a popular approach to health care. A study presented by American Hearth Association showing that brown rice can lower cholesterol level in bood serum. To know the benefi ts of Angkak?s steeping water to decrease serum cholesterol levels in mice and determine the effects of Angkak?s steeping water to decrease serum cholesterol levels in rats compared with simvastatin. This study used an experimental research design, and using pre and post test control group design, performed in the laboratory of biomedical III FK UMS on November 1, 2012 to date of January 1, 2013. The purposive sampling technique, sampel determinaton using formula Federer by the number of sample of 30 male white rats wistar strain. After the data is collected, analyzed using the Shapiro-Wilk test, ANOVA, LSD, and the unpaired t test. The result of this study demnstrate hypothesis testing using ANOVA test with signifi cant level 95% of the negative control group, the treatment group a dose of 1, 2, and 3, it was found p<0,05 were signifi cantly different meaning. In the test dose of LSD between treatment groups 2 and 3 the value of p=0,839 (p>0,05) wich means no signifi cant difference. In the unpaired T test between the treatment group a dose of 1, 2, and 3 with positive control group were signifi cant difference. The unpaired T test between the treatment group a dose of 1, 2, and 3 with white rice also found signifi cant difference. Conclusion: Angkak?s steeping water can lower serum cholesterol levels of white rat as well as the water steepimg Angkak has the same effectiveness with simvastatin to decrease serum cholesterol levels of mice.Keyword: Cholesterol, Angkak
STUDI PERBANDINGAN DUA KELOMPOK UMUR TERHADAP MULA KERJA DAN MASA KERJA ANESTETIKA LOKALPADA KASUS PENCABUTAN GIGI MOLAR I ATAU MOLAR II ATAS Kholifa, Mahmud
Biomedika Vol 3, No 2 (2011): Biomedika Agustus 2011
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v3i2.248

Abstract

Effect of in? ltration due to the action of anesthesiasubstance on terminal nerve ? bers maxillary molars the anesthesia? uid ? ow through the bone viahavers channels.This study is a quasi-experimental studies (clinical trials), which aims to determine the difference of onset and durationLidocaine between age groups 15-24 years age group 25-34 years in the case of extraction a molar tooth I or II maxilla conducted on patients who came to the Polyclinic FKG UGM, Yogyakarta. The number of samples obtained was 34 samples divided into two groups, Group I were patients with 15-24 years of age or younger, group II is patients aged 25-34 years or older. The data obtained areonset and duration of the age group 15-24 years compared with onset and duration of Lidocaine in the age group 25-34 years. The data obtained were analyzed by t-test with signi?cance level (p <0.05). The results of this study indicate that there are no signi? cant differences between the onsetLidocaine in the age group 15-24 years with onset on the Lidocaine group 25-34 years. There is also a non-signi? cant difference between Lidocaineduration in the group aged 15 - 24 years with a duration of Lidocaine on the group 25-34 years at p <0.05. The mean - median onsetLidocaine in the age group 15-24 years was 60.3 seconds with a range between 22-95 seconds while the onset on the Lidocaine group 25-34 years was 55.5 seconds with a range between 14-111 seconds. The mean - average durationLidocaine in the age group 15-24 years was 162.59 minutes with a range between 120-195 minutes while the duration of Lidocaine on the group 25-34 years was 184.41 with a range between 120-330 minutes.
BESAR KURVA THORAKAL DAN LUMBAL MODIFIER SEBAGAI FAKTOR PREDIKSI TERHADAP KOREKSI SPONTAN KURVA LUMBAL PASKA OPERASI PADA ADOLESCENT IDIOPATHIC SCOLIOSIS LENKE I Yurisworo, Anggita Tri; Widhiarso, Bagas; Prijosedjati, Andhi; Utomo, Pamudji
Biomedika Vol 11, No 2 (2019): Biomedika Agustus 2019
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v11i2.7629

Abstract

Adolescent idiopathic scoliosis Lenke I dengan pola kurva mayor pada kurva thorakal (main thoracic), dengan kurva proximal thoracic dan thoracolumbar/lumbar sebagai kurva minor non struktural. Instrumentasi dan fusi hanya pada kurva thorakal dianjurkan. Pada literatur dijelaskan bahwa koreksi spontan kurva lumbal terjadi sebagai kompensasi untuk menyeimbangkan kurva thorakal setelah dilakukannya fusi thorakal selektif. Lumbal modifier dibagi menjadi 3 grup, pemilihan terapi operatif bergantung pada tipe lumbal modifier. Penelitian ini merupakan suatu analisis observational pada 35 pasien adolescent idiopathic scoliosis Lenke I paska operasi koreksi deformitas dan instrumentasi posterior. evaluasi menggunakan X ray sebelum dan setelah operasi untuk penentuan tipe lumbal modifier, besarnya koreksi kurva thorakal dan penilaian koreksi spontan kurva lumbal, kemudian dilakukan uji korelasi terhadap data yang didapat. Besarnya koreksi kurva thorakal terhadap koreksi spontan kurva lumbal paska operasi memiliki nilai signifikansi (Sig) = 0,000 < 0,05 dan besarnya koreksi kurva thorakal memiliki nilai koefisien regresi lebih besar bila dibandingkan dengan lumbal modifier terhadap koreksi spontan kurva lumbal paska operasi (variabel besar koreksi kurva thorakal (X1) = 0,764, Lumbal modifier (X2) = 0,092). Besarnya koreksi kurva thorakal berpengaruh signifikan terhadap koreksi spontan kurva lumbal paska operasi dan besarnya koreksi kurva thorakal yang paling dominan dalam mempengaruhi koreksi spontan kurva lumbal dibandingkan dengan lumbal modifier.Kata kunci : Adolescent idiopathic scoliosis, Lumbal modifier, kurva thorakal Adolescent idiopathic scoliosis Lenke I, main thoracic curve pattern has the major curve, with the proximal thoracic and thoracolumbar/lumbar being non structural minor curves. Thus, instrumentation and fusion of the main thoracic region alone is recommended. In the literature it is said that the lumbar curve spontaneously corrects to balance the thoracic curve after selective thoracic fusion. Most authors assumed a mechanism whereby improvement of the lumbar curve occurred through counterbalancing the surgical correction of the thoracic curve. The lumbar curve divided in three subgroups: lumbar modifier A, B and C. The selection of specific operative treatments depends on this modifier. This study was an observational analysis in 35 adolescent idiopathic scoliosis Lenke I patients after correction and posterior instrumentation surgery. Evaluation using X-rays before and after surgery to determine the type of lumbar modifier, the magnitude of the main thoracic curve and assessment of spontaneous lumbar curve correction, then correlation test obtained. The magnitude of main thoracic curve correction to the spontaneous lumbar curve correction has a significance value (Sig) = 0.000 < 0.05 and the magnitude of main thoracic curve correction has a greater regression coefficient than the lumbar modifier for spontaneous lumbar curve correction (large correction variable thoracic curve (X1) = 0.764, Lumbal modifier (X2) = 0.092). The magnitude of main thoracic curve correction has a significant effect on the spontaneous lumbar curve correction and the magnitude of main thoracic curve correction is most dominant factor to influence spontaneous lumbar curve correction compared to the lumbar modifier.Keywords: Adolescent idiopathic scoliosis, Lumbar modifier, main thoracic
EFEK LARVISIDAL EKSTRAK ETANOL DAUN KEMANGI (Ocimumsanctum Linn) TERHADAP LARVA INSTAR III Culexquinquefasciatus LA, Wijayani; S, Isti?anah
Biomedika Vol 6, No 2 (2014): Biomedika Agustus 2014
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v6i2.275

Abstract

Filariasis carried by mosquito Culex quinquefasciatus. Filariasis cause elephantiasis sequelae that often lower the sufferers productivity . Eradication of the disease is difficult, then , an attempt to do is to control the vector. The other way is to use insecticide vector control plant. Basil (Ocimum sanctum Linn) which contain fiavonoids, saponins, and eugenol has been shown in the laboratory have larvicidal effect. This study aimed to determine the larvicidal effects from Ethanol extract 96% of basilleaf (Ocimum sanctum Linn) against third instar larvae of Culex quinquefasciatus. This is an laboratory experimental methods with Post test only control group design. The Ethanol extract of basil leaf (Ocimum sanctum Linn) diluted to a concentration of 5000 ppm, 4500 ppm, 4000 ppm, 3500 ppm, 3000 ppm, 2000 ppm, 1500 ppm, 1000 ppm. The experiments were repeated three times. Each experiment included a control ie Tween 20 of 0.25% in distilled water. Statistical analysis was performed with Kruskal-Walls test and probit analysis to determine LC50 and LC90. In the analysis result of Kruskal Walls (CI: 95%) it obtained the results that each test group had a significant difference (p=0,005) in mortality of third instar larvae of Culex quinquefasciatus.The results of probit analysis showed that the ethanol extract of basil leaf give the concentration LC50 and LC90 2281.931 ppm and 5016.314 ppm. Ethanol extract 96% of basilleaf (Ocimum sanctum Linn) has an larvicidal effect on third instar larvae of Culex quinquefasciatus.Keywords: Culex quinquefasciatus - larvicidal - ethanol extract - Basilleaf (Ocimum sanctum Linn).
EKSPRESI ANTI-HELICOBACTER PYLORI PADA GASTRITIS KRONIS, LESI PRAKANKER, DAN KARSINOMA GASTER Damayanti, Lina; Putranto, Bambang Hendro; Sadhana, Udadi
Biomedika Vol 7, No 2 (2015): Biomedika Agustus 2015
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v7i2.1896

Abstract

Prevalence of Helicobacter pylori infection in Indonesia was 36-46%. In Jakarta and Surabaya, the prevalence were 85,7%-93,9%. Helicobacter pylori infection play role in pathogenesis of pectic ulcers, chronic gastritis, carcinoma of gaster and gastric lymphoma. Epidemiologic study showed 80% of carcinoma of gaster related with H pylori infection.This study analyzed expression of anti-Helicobacter pylori in chronic gastritis, precancer lesion , and carcinoma of gaster. This study was a observational descriptive study with case control design. Thirty (30) samples from paraffin bloc that were diagnosed with chronic gastritis, precancer lesion, and carcinoma of gaster at Dokter Kariadi hospital in 2013 was stained by hematoxylin eosin, giemsa and immunohistochemistry of anti-helicobacter pylori. Data was analyzed by descriptive analysis. Thirty (30) samples were diagnosed as gastritis chronis 13 (43,3% ), pra cancer lesion(36.6%), and carcinoma(20.1%). Chronic gastritis can be occurred at all age and no distinct difference on sex, while gastric carcinoma predominant in male older than 40 years. Expresion of Helicobacter pylori on chronic gastritis was 84.6%, precancer lesion was 54.5%, and gastric carcinoma was 83.3%. The Giemsa stain gave 23.3% false positive and 20% false negative. Helicobacter pylori expression can be showed in chronic gastritis, precancer lesion, and gastric carcinoma.Keywords: Chronic gastritis, gastric carcinoma, Helicobacter pylori
HUBUNGAN TINGKAT PENDAPATAN KELUARGA DENGAN STATUS GIZI BALITA DI WILAYAH KERJA PUSKESMAS KALIJAMBE Handini, Dian; Ichsan, Burhannudin; Nirlawati, Dona Dewi
Biomedika Vol 5, No 2 (2013): Biomedika Agustus 2013
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v5i2.263

Abstract

The state of nutritional status of children in Central Java based on index of height for age data obtained 16.9% of children who have growth in a very short, short 17.0% of infants and 66.1% of normal infants. Economic growth in Sragen in 2000 was 2.85%, decreased in 2001 to 2.26%, and increased in 2002-2003, respectively 2.93% and 3.26%. Research methode was observational cross sectional analytic approach. The number of samples was 65 toddlers. Data analysis using Chi Square. The nutritional status of children by index weight for age (W/A) were experiencing malnutrition while the remaining 13.8% as having good nutrition as much as 86.2%, and the index based on height for age (H/A) which had malnutrition as much as 46.2% while the rest had good nutrition as much as 53.8%, while the index by weight according to height (W/H) were experiencing malnutrition while the remaining 13.8% as having good nutrition as much as 86.2% . The results of the analysis obtained p count is 0.009 to the sample distribution based on W/A, and 0.010 for sample distribution based H/A, and 0.009 for the distribution of the sample by W/H. There is a relationship between the level of family income to the nutritional status of children in the working area Public Health Center Kalijambe.Keywords: Family Income, Nutritional Status of Children
UJI EFEK ANTIBAKTERI EKSTRAK ETIL ASETAT DAN KLOROFORM MENIRAN (Phyllanthus niruri Linn) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 6538 DAN Escherichia coli ATCC 11229 SECARA in vitro Rahman, Dwiariawan Tauchid; Sutrisna, EM; Candrasari, Anika
Biomedika Vol 4, No 2 (2012): Biomedika Agustus 2012
Publisher : Universitas Muhamadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/biomedika.v4i2.254

Abstract

Meniran is one of traditional plants that contains several compounds such as terpenoid, flavonoid, and alkaloid that role as antibactrial agent. The aims of the research are to determine the antibacteria effect of extract aethyl acetat and chloroform of meniran. This research was conducted as a laboratory experimental by using post test control group design only. The concentration of extract aethyl acetat and chloroform meniran are 5%, 10%, 20%, 40%, and 80%. The result indicate that extract aethyl acetat of meniran have not effect to Escherichia coli. Meanwhile, to Staphyloccus aureus have an effect (p=0,003). The concentration of the extract chloroform meniran are effective to inhibite the growth of Escherichia coli (p=0,029) and Staphylococcus aureus (p=0,004). The extract aethyl acetat of meniran have an antibacteria effect to Staphylococcus aureus but not to Escherichia coli. Whereas, the extract chloroform of meniran have an antibacteria effect to Staphylococcus aureus and Escherichia coli.Keyword : The aethyl acetat and chloroform extract of Meniran (Phyllanthus niruri L.), antibacteria effect, Staphylococcus aureus and Escherichia coli

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