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Y. Andi Trisyono
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 509 Documents
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Paraquat Toxicity on the Growth of Rhizobium sp. in a Synthetic Medium Erni Martani
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 2 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12366

Abstract

Toxicity of paraquat on the growth of several strains of Rhizobium sp. in Yeast Extract Mannitol medium was studied. Various concentrations of paraquat 100, ranged from O (control) to 100 ppm were applied. Qualitative examination was done using paper disc diffusion technique, and the quantitative examination was conducted based on the change in cell density in medium measured by plate count method.Qualitative data showed that effect or paraquat was species specific. Some strains of Rhizobium sp., namely Rhizobium sp. strain T-37 and QF, were tolerant to paraquat until100 ppm, but other strains were sensitive to paraquat, especially at high concentration.Quantitative examination to the sensitive strains shows that higher concentration of paraquat caused higher toxicity to the growth of Rhizobium. Rhizobium sp. strain G-69 and  G-182.paraquat addition at 100 ppm slightly decreased cell density from 10 to 10 CFU/mL. Rhizobium japonicum strains 143 and KS were tolerant to 20 and 40 ppm of paraquat; their cell density increased from 10^6 to a level of 10&7 or 10^8 CFU/mL depend on the strain. This level was not significantly lower than those in medium without paraquat, in which maximal population density reached to 10^8 or 10^9 CFU/mL. Addition of higher paraquat concentration damaged the cell of these strains, and caused population density increased specifically to a level of 10^1 CFU/mL. The data which showed that paraquat was toxic to Rhizobium sp. were important growth inhibitor, because growth inhibition of these bacteria may influence the formation of root nodule on leguminous plants, and in turn will decrease the yield. Due to widely applied paraquat in agricultural and plantation systems, and the role of Rhizobium in nitrigen fixation, these results are important for minimizing the impact of paraquat application.
Tingkat Parasitasi Fopius arisanus (Hymenoptera : Braconidae) pada Lalat Buah Belimbing di Daerah Istimewa Yogyakarta Suputa Suputa; Ahmad Taufiq Arminudin; Palupi Jatuasri; Ika Puji Rahmawati; Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11855

Abstract

Bactrocera carambolae was founf on carambola fruit in Yogyakarta Special Province and there were three species of parasitoids, i.e. Fopius arisanus, Agasnaspis sp., and Asobara sp. The population of Agasnaspis sp. and Asobara sp. were very low and was only found in Samas coastal area. F. arisanus was dominant and always found in all observation sites. There was no significant result on their parasitism (for region, F(2,35) = 0.057; p>0.005; for elevation, F(2,35)= 0.704, p>0.05; for habitat, F(2,35)= 0.215, p>0.05). Parasitism of F. arisanus on fruit fly in Yogyakarta Special Province was generally low, i.e. it ranged from 0.5495 ± 0.3843 (in Sleman), to 1.2935 ± 0.8206%. Evaluation of the existence of F. arisanus and augmentation efforts might be needed to improve its ability to parasitize fruit fly in Yogyakarta Special Province.
Ketahanan Ubi Jalar terhadap Penyakit Kudis Eko Agus Martanto; Haryono Semangun; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 3, No 2 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6238.02 KB) | DOI: 10.22146/jpti.12959

Abstract

The experiment was conducted to know the resistance of sweet potato cultivars to scab caused by Elsinoe batatas, the incubation period, and the influence of stomatal density to the resistance of the host. The test showed that Malothok, Mlg 12538, and Prambanan cultivars were moderately resistant, while Ciceh-5 was resistant. The incubation period on Ciceh-5 was longer than that on the other moderately resistant cultivars. On resistant cultivar, stomatal density was lower than that on moderately resistant cultivars.
Kemampuan Pseudomonas putida Pf-20 dan 24.7B untuk Memperbaiki Sifat Kimia Media Tumbuh dan Ketahanan Terinduksi Tembakau H877 terhadap Cucumber mosaic virus W. S. Wahyuni; A. Iwan; A. Mudjiharjati; T. C. Setyowati; H. Purwiko
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12085

Abstract

P. putida Pf-20 from medium M1 (paddy soil, leaves compost and manure compost, 1:3:2) has a higher affinity and more rapid to solubilize phosphate on Pikovskaya medium, rather than P. putida 27.4B. Those affinities of both strains from the media M1 with CMV-48 were much greater than that of medium without CMV. After 64 days planted, the content of N total, C-organic, CEC, K20, and P205 were better on medium M1, but the pH was little bit decreased. The reduction of P04 and Fe in medium M1 could due to the bacterial growth, and for producing siderophore, particularly on the media with viral treatment. These bacteria strains did not affect on the plant height and number of leaves on media M1, M2 or M3, however, these bacteria affected on the total root length and root densities in the medium M1 with virus. The more available nutrient in the medium, the more rapid bacteria colonized rhizosphere and roots. Both of these bacteria were effective to reduce the disease severity of CMV on tobacco H877. The medium M1 was the best medium for bacterial and tobacco growth.
The Effect of Bacillus thuringiensis toxin Cry1A.105 and Cry2Ab2 on The Survival of The Non-Target Pest, Spodoptera litura Kurnia Pratiwi; Y. Andi Trisyono; Edhi Martono
Jurnal Perlindungan Tanaman Indonesia Vol 20, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (17.235 KB) | DOI: 10.22146/jpti.16618

Abstract

Spodoptera litura is one of the important insect pest of maize besides the notoriously damaging corn borer, Ostrinia furnacalis. S. litura has been the target of various controls including the use of Bacillus thuringiensis (Bt) toxin Cry1A.105 and Cry2Ab2. This study was conducted to evaluate the acute effect of Bt toxin Cry1A.105 and Cry2Ab2 on the growth and development of S. litura from larval to adult stages. Two sublethal concentrations were used; 0.1875 and 0.0469 ppm for Cry1A.105, and 0.0008 and 0.0003 ppm for Cry2Ab2. The bioassay using diet dipping was carried out on a CRD with three experiments and five repetitions. The observation was carried out on the mortality and survival rates of S. litura. The mortality reached 28% when the larvae were treated with 0.1875 ppm and 20% with 0.0469 ppm of Cry 1A.105. The exposed larvae and pupae were smaller than control. Larval and pupal weight were 117.0 and 165.6 g with 0.1875 ppm, while control were 212.9 and 211.2 g. Cry1A.105 also longer the larval stage, larval stage with higher and lower concentration were 24.5 and 22.3 day, while control was 20.5 day. The resulted pupae from larve which exposed by Cry1A.105 were less than control; there were 40% at concentration 0.1875 ppm and control 61%. The two concentration of Cry2Ab2 produced similar mortality of 20%. Similarly, Cry2Ab2 affected pupal to adult stages development. The longevity of pupal stage with concentration 0.0003 ppm was 9.5 days, followed by 0.0008 ppm (9.1 days) and control (10.1 days). The adult emerge on the highest concentration was 47.4% while control only 34.6%. There results showed that both Cry1A.105 and Cry2Ab2 were detrimental to the survival of S. litura which is the non-target insect of transgenic Bt maize. INTISARI Spodoptera litura merupakan salah satu hama penting yang menyerang tanaman jagung, selain Ostrinia furnacalis. Belakangan ini O. furnacalis diketahui telah menjadi target dari berbagai macam cara pengendalian termasuk penggunaan toksin Bacillus thuringiensis (Bt) Cry1A.105 dan Cry2Ab2. Penelitian ini dilakukan untuk mengetahui efek akut toksin Bt Cry1A.105 dan Cry2Ab2 terhadap pertumbuhan dan perkembangan S. litura dari larva sampai imago. Dua konsentrasi subletal yang akan digunakan adalah; 0,1875 dan 0,0469 ppm untuk Cry1A.105, dan 0,0008 dan 0,0003 ppm untuk Cry2Ab2. Pengujian dilakukan dengan menggunakan metode celup pakan dan Rancangan Acak Legkap dengan tiga perlakuan dan lima ulangan. Pengamatan dilakukan terhadap mortalitas dan kelangsungan hidup S. litura. Mortalitas mencapai 28% pada larva yang dipaparkan dengan 0,1875 ppm dan 20% dengan 0,0469 ppm Cry 1A.105. Larva dan pupa yang terkena toksin berukuran lebih kecil. Berat larva dan pupa yang terpapar toksin dengan konsentrasi 0,1875 ppm, masing – masing 117,0 dan 165,6 g, sedangkan kontrol masing – masing 212,9 dan 211,2 g. Cry1A.105 juga dapat memperpanjang stadia larva. Lama stadia larva dengan konsentrasi tertinggi dan terendah adalah 24,5 dan 22,3 hari, sedangkan kontrol 20,5 hari. Jumlah pupa yang berhasil terbentuk dari larva yang terpapar toxin Cry1A.105 lebih sedikit dibandingkan dengan kontrol; pada konsentrasi 0,1875 ppm sebesar 40%, sedangkan kontrol sebesar 61%. Kedua konsentrasi dari toksin Bt Cry2Ab2 menyebabkan mortalitas yang sama yaitu 20%. Cry2Ab2 juga berpengaruh terhadap lama stadia pupa dan tingkat keberhasilan pembentukan imago. Lama stadia pupa dengan konsentrasi 0,0003 ppm adalah 9,5 hari, diikuti dengan konsentrasi 0,0008 ppm (9,1 hari) dan kontrol (10,1 hari). Jumlah imago terbanyak terdapat pada perlakuan dengan konsentrasi 0,0008 ppm sebesar 47,4% sedangkan pada kontrol hanya 34,6%. Hal ini menunjukkan bahwa toksin Bt Cry1A. 105 dan Cry2Ab2 juga berpengaruh terhadap kelangsungan hidup S. litura yang merupakan serangga bukan sasaran dari tanaman jagung transgenik Bt.
Ledakan Populasi Jenis Respo, Filicaulis bleekeri, di Sentra Produksi Sayur Rejang Lebong, Bengkulu Dwinardi Apriyanto; B. Toha; I. Manti
Jurnal Perlindungan Tanaman Indonesia Vol 9, No 1 (2003)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12278

Abstract

During the last two years a slug species, Filicaulis bleekeri (Keferst.), has become important pest of some vegetable crops in vegetable production central Rejang Lebong,Bengkulu. This pest is called "respo" by local farmers, who majority are Javanese. Data from preliminary observation indicated that this pest population was very high and may caused harvest failure on some vegetable crops, particularly Cruciferae such as cabbage, chinese cabbage, and cauliflower. In several sampling activities in 2000 and 2001, the population density of respo reached >10 per mature cabbage plant and >7 per flowered cauliflower plant. Sampling on young cabbage and chinese cabbage resulted >2 respo per 1 m of row, but killed >80% of transplanted cabbage and 75% of transplanted chinese cabbage. Whereas sampling in fallow land resulted >60 respo per m2. There is no much information on this pest presently; therefore it is necessary to study the biology as well technology and as control strategy of the species.
Peningkatan Ketahanan Tanaman Pisang Kepok Kuning terhadap Penyakit Darah melalui Variasi Somaklonal dan Simbiosis Endofitik Arif Wibowo; Tri Joko; Siti Subandiyah; Ika Mariska; Yati Supriyati; Yadi Suryadi; Ika Roostika
Jurnal Perlindungan Tanaman Indonesia Vol 16, No 1 (2010)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3699.305 KB) | DOI: 10.22146/jpti.11738

Abstract

One of the obstacles that was encountered in the banana cultivation is blood disease. Blood diseases is caused by Ralstonia solanacearum that is subsequently revised to become blood disease bacteria (BDB). Until now the control of banana blood disease is very difficult. Control of banana blood disease with chemical injections and soil treatment is not effective. This study was aimed to obtain Kepok Kuning cultivar banana seedlings which was resistant towards blood disease obtained from in vitro selection by using BDB growing filtrate and induced resistance by inoculation of antagonistic endophytic bacteria.The observation of Kepok Kuning banana explants treated with various concentrations of BDB growing filtrate showed that the percentage of living explants decreased to 83.33% when the BDB growing filtrate concentration increased to 15%. Treatment of banana explants with BDB growing filtrate also affected the number of roots, shoots, and leaves. Treatment with a single antagonistic endophytic bacteria suppressed the intensity of banana blood disease to 0% in comparison with the mixture of antagonistic endophytic bacteria if Kepok Kuning banana explants were not treated with BDB growing filtrate. When Kepok Kuning banana explants were treated with BDB growing filtrate, the intensity of banana blood disease suppressed to 0% after the high concentration of BDB growing filtrate and the antagonistic endophytic bacteria mixture were applied.
Penelitian Pendahuluan Pengaruh Daun Manggis sebagai Rodentisida Nabati pada Mencit Mus musculus Strain Balepsi Agus Kardinan; Momo Iskandar; Mukhasim Mukhasim
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (9707.208 KB) | DOI: 10.22146/jpti.12409

Abstract

Research has been conducted at Pest and Disease Laboratory, Research Institute for Spices and Medicinal Crops, Bogor, during 1997-1998. The objective was to evaluate the possibility of Mangosteen (Garcinia mangostana) leaves to be used as botanical rodenticide which is tested to reproduction ability of mice Mus musculus. Eight to ten weeks old mice with the average weight of 25 gram were obtained from Research Institute for Livestock, Ciawi. Research was arranged at randomized block design, 8 treatments and 4 replications. Data was analyzed by determining the average value and their standard deviation values. Observations were done to the weight of testis, weight of embryo, number of embryo, volume of extracts drank by mice and mice behavior during experiment. Result revealed that there was no indication that the leaves extract affected the weight of testis, but it affected the number and weight of embryo. There was indication that mangosteen leaves acted as antifertility on mice. Giving leaves extract increased the total volume of extract drink. There was no abnormality at the activity of mice during experiment, included sex activity, poisonous symptoms and others.
Kisaran Inang dan Keragaman Gejala Infeksi Turnip Mosaic Virus Eliza Suryati Rusli; Sri Hendrastuti Hidayat; Gede Suastika; Utomo Kartosuwondo
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 1 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11779

Abstract

The incidence of mosaic disease on vegetable crops in Indonesia has been reported recently. The disease is caused by TuMV which is considered as a new and important virus on caisin and turnip in Indonesia. Field survey has been conducted to determine disease incidence in vegetable growing areas. Symptom variability and host range of TuMV was further studied through mechanical inoculation to cruciferae and solanaceae plants. Observation during field survey has proved that TuMV has infected caisin and turnip in Java and Bali. The highest intensity of mosaic disease i.e. 63,3% occurs in Tumpangan-Malang, followed by Denpasar Selatan and Bandungan-Semarang with the intensity of 30,5% and 19,0% respectively. TuMV infection causes different types of symptoms, such as: wrinkled leaf, blistered leaf, vein banding, vein clearing, leaf distortion and proliferation. The host range of TuMV involves those plants belong to cruciferae (cabbage, broccoli, caisin, turnip, cauliflower, chinese cabbage, pak coy); solanaceae (N. tabacum, N. benthamiana, N. glutinosa); and chenopodiaceae (C. amaranticolor). Furthermore, N. glutinosa can be used as differential host for TuMV isolates.
Pengaruh Ekstrak Daun Mimba terhadap Perkembangan Antraknos pada Apel Manalagi Pascapanen Ika Roostika Tambunan; Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 3, No 1 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4975.36 KB) | DOI: 10.22146/jpti.12904

Abstract

Neem leaf extract inhibitss spore germination of Colletotrichum gloeosporioides. Leaf extract obtained by Soxhlet method is better than leaf extract obtained by boiling water method. The extract inhibits development of anthracnose disease on manalagi apple up to nine days after spraying.

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