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Contact Name
Y. Andi Trisyono
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anditrisyono@ugm.ac.id
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+62274-523926
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jpti.faperta@ugm.ac.id
Editorial Address
Jalan Flora No. 1, Bulaksumur, Sleman, Yogyakarta, 55281
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Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Jurnal Perlindungan Tanaman Indonesia
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Arjuna Subject : -
Articles 509 Documents
Pewarisan Ketahanan Melon (Cucumis melo L.) Kultivar Melodi Gama 3 terhadap Kyuri green mottle mosaic virus Budi Setiadi Daryono; Faizatul Fitriyah
Jurnal Perlindungan Tanaman Indonesia Vol 20, No 2 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.17702

Abstract

Melon (Cucumis melo L.) belongs to Cucurbitaceae. Melon has high potential to be developed as main horticultural product in Indonesia. Melon is one of important foreign exchange and is the fifth biggest horticulture commodity in Indonesia. One of the problems in melon farming is mosaic disease caused by Kyuri green mottle mosaic virus (KGMMV). KGMMV infection reduces the quality and the amount of melon production. Melon farmers suffered a significant financial loss. Melodi Gama 3 (MG3) is a high yielding melon cultivar from the Genetics Laboratory, Faculty of Biology, Universitas Gadjah Mada. The use of genetically resistant melon cultivar has beneficial outcome for agriculture sector. The aim of this research was to study the resistance’s inherintance to KGMMV in MG3 melon cultivar. Two cultivars of MG3, MG3|5and MG3|8, were cultivated in the greenhouse. MAI, Glamour, Ladika, and Action melon cultivars were used as references. Resistance of KGMMV was analyzed by symptom observation and serological detection using Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA). DAS-ELISA result analyzed further to establish resistance category. Description to melon cultivar phenotype variation was done. The result of this research indicates that MG3 melon cultivar is tolerant to KGMMV. The decrease of MG3 optical density was directly related with the lowering of KGMMV symptoms. The character of tolerance to KGMMV was inherited from Melodi Gama 1 (MG1) cultivar. INTISARI Melon (Cucumis melo L.) merupakan tanaman buah yang tergolong dalam familia Cucurbitaceae. Tanaman melon berpotensi untuk dikembangkan sebagai produk unggulan hortikultura di Indonesia. Tanaman melon juga merupakan salah satu penghasil devisa penting Indonesia dan menempati urutan ke-5 dari kelompok hortikultura. Salah satu kendala yang sering dihadapi oleh petani melon adalah penyakit mosaik yang disebabkan oleh Kyuri green mottle mosaic virus (KGMMV). Infeksi KGMMV pada pertanian melon mengakibatkan penurunan kualitas dan kuantitas hasil, sehingga petani mengalami kerugian ekonomi yang cukup berarti. Melodi Gama 3 (MG3) merupakan kultivar melon unggul hasil rakitan Laboratorium Genetika, Fakultas Biologi, Universitas Gadjah Mada. Penggunaan kultivar melon yang tahan terhadap infeksi KGMMV secara genetis merupakan alternatif yang sangat bermanfaat dalam bidang pertanian. Penelitian ini dilakukan untuk mengetahui pewarisan ketahanan MG3 terhadap infeksi KGMMV. Melon kultivar MG3, ditumbuhkan di greenhouse. Sebagai pembanding digunakan melon kultivar yang umum ditanam petani, yaitu MAI, Glamour, Ladika, dan Action. Kelima kultivar melon tersebut diinokulasi dengan KGMMV. Parameter ketahanan KGMMV yang digunakan adalah segregasi gejala dan uji serologis dengan Double Antibody Sandwich Enzyme Linked Immunosorbent Assay (DAS-ELISA). Hasil DAS-ELISA selanjutnya dianalisis untuk mengetahui kategori ketahanannya. Dilakukan pula deskripsi pada variasi fenotip kultivar melon yang ditanam. Hasil penelitian ini menunjukkan bahwa tanaman melon kultivar Melodi Gama 3 memiliki sifat toleransi terhadap infeksi KGMMV. Toleransi ditunjukkan dengan nilai optical density (OD) yang menurun seiring dengan penurunan gejala infeksi KGMMV. Sifat ketahanan terhadap KGMMV diwariskan dari kultivar Melodi Gama 1 (MG1).
Pengelolaan Lingkungan dalam Rangka Mengantisipasi Perkembangan Hama Belalang Nomadacris succincta Linnaeus (Orthoptera: Acrididae) Sri Suharni Siwi; Trisnaningsih Trisnaningsih; Harnoto Harnoto
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 2 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12102

Abstract

Swarming grasshopper Nomadacris succincta (Linnaeus), sinonim: Patanga succincta (L.) was one of the dominant species attacked corn and soybean plantations at Jeneponto District, South Sulawesi in early 2005. The species has been identified for a long time occurred in that environment, although has never been reported before as pest of farmer's crops. For a basis of the capability in controlling the swarm grasshopper in the field, changed of increasing population hould be monitored regularly to prevent future outbreak. Information on taxonomic and ecology research of N. succincta are needed for environmental management efforts, such as change in morphology, physiology and behavior, change in host's preferences of gregarious adult, and environmental conditions i.e. climate, cultural techniques, and the death of natural enemies in the field.
Pewarisan Sifat Ketahanan Tanaman Melon (Cucumis melo L.) terhadap Powdery Mildew (Podosphaera xanthii (Castag.) Braun et Shishkoff) Budi Setiadi Daryono; Muhammad Taufiq Qurrohman
Jurnal Perlindungan Tanaman Indonesia Vol 15, No 1 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11758

Abstract

Powdery mildew is a fungal disease that causes subtantial losses in melon production around the world including Indonesia. Resistant melon cultivar to powdery mildew is important to increase melon production. Hence, the objectives of this study were to study melon resistance against powdery mildew and its inheritance. A F2 population was obtained from self pollination between F1 ♀ 371795 which derived from ♀ PI 371795 X ♂Action 434 and F1 ♀Action 434 which derived from ♀ Action 434 X ♂ PI 371795. In addition, a test cross population was produce by crossing between F1 with Action 434 as a recessive homozygote pa-rent. Furthermore, powdery mildew resistance in a F1, F2 and test cross populations were tested by inoculating powdery mildew which collected from Ngawi regency. The results were evaluated by Chi-square test (χ2). The results showed that a F1, F2 and test cross populations have different resistance levels to powdery mildew. The resistance to powdery mildew in a F2 ♀ PI 371795 population showed segregation and have 3:1 ratio of Mendel inheritance pattern. On the other hand, resistance to powdery mildew in a test cross with PI 371795 as a parent showed segregation with 1:1 ratio. While resistance in a F2 ♀ Action 434 and test cross populations with Action 434 as a parent did not express Mendel inheritance pattern. Based on the result it could be concluded thatn resistance to powdery mildew in PI 371795 as a female parent is controlled by a single dominant gene (pm-I), while resistance to powdery mildew in action 434 as a female parent is suppose to be controlled by several genes (oligogene). 
Total Protein Content and Pathogenesis Related-Proteins in Leaf Intercellular Fluid of Cigar Tobacco Cultivar H-877 Infected with Tobacco Mosaic Virus (TMV) Wiwiek Sri Wahyuni; Endang Budi Trisusilowati; Didiek Sulistyanto
Jurnal Perlindungan Tanaman Indonesia Vol 5, No 2 (1999)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12758

Abstract

The reaction of hypersensitive Nicotiana species or cultivar (N. glutinosa, N. tabacum cv. Xanthi nc., N. tabacum cv. H-877) to three isolates of TMV from Jember showed variable symptoms. Pattern of total protein from leaf was hard to distinguish between cultivar or species. The intercellular fluids (IF) of leaf tissues which contained pathogenesis related (PR) proteins of cigar tobacco cultivar H-877 (it derives the N-gene from N. glutinosa) has lost several protein bands than that of N. glutinosa. The H-382 cultivar which systemic reaction to TMV has a band with very low molecular weight, which was not detected in other species or cultivar infected with three TMV isolates. It seems there was an effect of longterm storage of leaf before IF-extraction to the resolution of some PR protein bands in SDS PAGE. Analysis of PR-proteins of H877 cultivar in 20-SDS PAGE showed it consist of b-4, b-5, b-6a, b-6b, b-7a, and b-8a proteins of Parent and Asselin(1984).
Hubungan Antara Aktivitas Poligalakturonase dengan Virulensi RAS 4 Fusarium oxysporum f.sp. cubense Arif Wibowo; Siti Subandiyah; Christanti Sumardiyono; Liliek Sulistyowati; Peter Taylor
Jurnal Perlindungan Tanaman Indonesia Vol 14, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11871

Abstract

One of the major constraints of banana plantation in Indonesia is the occurrence of fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense. The pathogen produced series of cell wall degradation extracellular enzymes which have important roles in pathogenicity. Many studies have been conducted to know the role of degrading enzyme banana pectin is the major component of cell wall. Many pectinolytic enzymes such as polygalacturonase and others have been isolated from many fungal plant pathogens. The study was aimed to know the role of polygalacturonase towards the virulence of race 4 of F. oxysporum f.sp. cubense. The result showed that from 10 isolates of race 4 of F. oxysporum f.sp. cubense, the most virulent isolate was Lmp1 followed by Srg1, Bgl6, Mln1, Bgl3, A13, Bnt2, Gnk3, Kjg1 dan Wsb5. This was indicated by high and low percentage of wilting leaves of banana cultivar Cavendish when they were inoculated with these isolates. Incubation period varied from 3 to 6 weeks after inoculation SDS-PAGE showed that polygalacturonase, mostly PG1 and PG2, was secreted by these isolates, whereas PG3 was only found in growing cultures of Gnk3 and Wsb5 isolates. Detection of polygalacturonase activity with diffusion agar and reducing sugar methods showed that the activity of polygalacturonase secreted by F. oxysporum f.sp. cubense in the growing culture had no correlation with the virulence of the fungal pathogen.
Pemanfaatan Membran Nitroselulosa untuk Pengiriman Antigen Uji dalam Deteksi TMV dengan DIBA Susamto Somowiyarjo; Y. B. Sumardiyono; Suharno Suharno
Jurnal Perlindungan Tanaman Indonesia Vol 3, No 1 (1997)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12969

Abstract

Simplification of dot immunobinding assay (DIBA) by using TMV-infected samples which were stored and mailed in nitrocellulose membrane (NCM) was described. The antigenicity of TMV in DIBA could be maintained in leaf extract-dotted NCM which had been stored at 29ºC al least for 42 days. The method was developed for sending samples using infected leaf extract-dotted NCM to replace fresh samples. By this method, the antigenicity of the virus could he detected after they have been sent to 18 places which took time from 7 to 26 days. It ts anticipated that the simplicity of DIBA using mailed samples will lead to DIBA's rapid adoption for development of central diagnosis facilities to support the viral diseases management. It may also have wider use in DIBA-based screening and survey programs for plant viruses and could overcome plant quarantine restriction.
Variasi Kebugaran Jenis/Strain Trichogramma pada Telur Plutella xylostella (L.) (Lepidoptera: Plutellidae) Siti Herlinda
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12115

Abstract

Laboratory experiments were conducted to evaluate fitness traits of trichogrammatid species/strains reared on eggs of the diamondback moth, Plutella xylostella (L.). The experiments used eleven trichogrammatid species/strains collected from different host species and locations. They were Trichogrammatoidea cojuangcoi Nagaraja collected from parasitized P. xylostella eggs in Jarai, Muarasiban, Kerinjing, and Bogar, Trichogrammatoidea sp. A and B collected from parasitized Helicoverpa armigera (Hubner) eggs in Bogor, Trichogrammatoidea armigera Nagaraja collected from parasitized H. armigera eggs in Bogor, Trichogramma chilonis Ishii, Trichogramma japanicum Ashmead, Trichogramma australicum Girault, and Trichogrammatoidea nana (Zehntner) collected from parasitized Chilo sp. eggs in Cinta Manis, South Sumatera. When a female of Trichogramma was constantly exposed to 100 P. xylostella eggs per day throughout her lifetime, T. cojuangcoi from Jarai produced more parasitized host eggs (50.67%) and progenies (50.67 adults/female) compared to other species/strains. Sex ratio was predominantly female, the most female progenies were produced ( 38 adults/ female) by T. cojuangcoi from Jarai. There were no significant differences in larval (0-3.27%) and pupal (0-3.75%) mortality of all species/strains. Survival emerging adults of T. japanicum (90.24%) were significantly lower than those of other species/strains. These data showed that T. cojuangcoi from Jarai could be developed as biocontrol agents of P. xylostella.
Obituari: Prof. (Emer.) Dr. Ir. Haryono Semangun Christanti Sumardiyono
Jurnal Perlindungan Tanaman Indonesia Vol 20, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.16497

Abstract

Seleksi Resistansi Wereng Batang Padi Coklat terhadap Insektisida Fipronil Melhanah Melhanah; Witjaksono Witjaksono; Y. A. Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 2 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12364

Abstract

The rice brown planthoper, Nilaparvata lugens Stal (Homoptera: Delphacidae), is one of  key pests on rice. The use of insecticides unwisely has caused N. lugens to develop resistance. The objective of this research was to determine the potency of N. lugens to develop resistance through laboratory, selections to fipronil, a relatively new insecticide.  A population of N. lugens was collected from rice field in Muntilan, Central Java during the outbreak in 2001. Selections were carried out every generation for four generations against third instars. Selection was started by dipping Cisadane rice seedlings in a solution of fipronil (50 ppm). The concentration was increased every generation, and the concentration used for the last selection (the fourth generation) was 250 ppm. To determine the rate of resistance development, biassays were carried out to estimate LC50 of fipronil against selected and non-selected populations of N. lugens, and their LC50 values were compared to that of the laboratory population. The laboratory population has been in the laboratory for 15 years without any insecticides exposure. The founding population was as susceptible as the laboratory population to fipronil because their LC50 values were similar. Even though the concentration for selections was increased from generation to generation, the survival rate of N. lugens increased steadily. After four generations of selection, the resistance of selected population was 27.3 times than the non-selected population. These findings indicate that N. lugens population has the potency to develop resistance to fipronil in a continuous selection pressure is applied.
Biologi Lalat Sciarid, Bradysia ocellaris (Diptera : Sciaridae), Serangga Hama pada Pertanaman Jamur Tiram Rostaman Rostaman; Agus Dana Permana; Tati S. Subahar; Soelaksono Sastrodihardjo
Jurnal Perlindungan Tanaman Indonesia Vol 13, No 2 (2007)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.11815

Abstract

Bradysia ocellaris Comstock is an important insect pest of oyster mushroom at Bandung. Eggs are oval, 0.44 ± 0.12 mm in length, 0.26 ± 0.08 mm in width, and translucent. Larvae are worm-like, legless, translucent, and capsule distinctive black. Larvae consist four instars. Pupae are obtectate, 2040 ± 0.22 mm in length, and 0.69 ± 0.10 mm in width. Adults are small, females 2.58 ± 0.28 mm and males 2021 ± 0.28 mm in length. Life cycle is 16 -6 26 days. Larvae feed on media, mycelia and fruit bodies.