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INDONESIA
Journal of Tropical Biodiversity and Biotechnology
Published by Universitas Gadjah Mada
ISSN : 25409573     EISSN : 25409581     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
Arjuna Subject : -
Articles 327 Documents
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Phytochemical and Pharmacological Activities of Curcuma purpurascens Blume, A Review Oktariani Pramiastuti; Subagus Wahyuono; Nanang Fakhrudin; Puji Astuti
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75891

Abstract

Curcuma sp. is generally used for medicine, starch sources, preservatives, dyes and cosmetics. The use of Curcuma spp. for medical has increased because there have been many studies related to its active ingredients, such as flavonoids, essential oils, tannins, quinones, and terpenoids, as well as pharmacological activities, including wound healing, antioxidants, antifungal, anticancer, gastroprotective, and hepatoprotective. Curcuma purpurascens Blume is a species of Curcuma from family Zingiberaceae and used for traditional medicine. This article focuses on reviewing the literatures on C. purpurascens and discussing its morphology, phytochemical content, and pharmacological aspects. The method used to review this article was by exploring several databases such as Scopus, Pub Med, and Google Scholar to identify and download original articles and research journals related to the morphology, phytochemical content, and biological activity of Curcuma purpurascens Blume. The result of this review will later provide information about the uses and presence of Curcuma purpurascens Blume which is still rarely studied so further study related to its pharmacological activity tests and active compound as natural medicines can be explored.
The Formula media in vitro Propagation and Conservation of Ludwigia sp. Endang Gati Lestari; Media Fitri Isma Nugraha; Rossa Yunita
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75947

Abstract

The aquatic plant "Red Malang” (Ludwigia sp.) has a fairly high economic value as an ornamental aquatic plant, so it has the potential to be developed. The growth of in vitro cultures in culture bottles is high-speed, so it is necessary to find a formula media to inhibit growth so that the frequency of subcultures is reduced. The current research aims to produce a formula media for shoot multiplication and in vitro culture conservation. The research was carried out at the ICABIOGRAD tissue culture laboratory from April 2020 to June 2021. Research activities included plant propagation, conservation, and regeneration after conservation. Plant material was using in the form of a culture collection in the ICABIOGRAD tissue culture laboratory, treatment media for propagation were BA (0; 0.1; 0.3; 0.5; 0.7 and 0.9 mg/L) + thidiazuron (TDZ) (0 and 0.1mg/L). For conservation were MS + BA medium (0 and 0.1 mg/L) + paclobutrazol (0; 0.1; 0.3; 0.5; 0.7 mg/L) and for shoot regeneration after conservation using MS medium without Plant Growth Regulator (PGR). Data analysis using the Anova SAS version 9.0 test program. Further test using DMRT test with alpha level 5%. There was no difference in the mean value between levels of TDZ treatment on the number of shoots and leaves. The difference in the mean value between levels of TDZ treatment was very significant on shoot height, the number of roots, and root length. BA treatment with a concentration of 0.7 mg/L is better because it gives higher results for each observation variable. For conservation, treatment with paclobutrazol 0.5 mg/L inhibited shoot and leaf count, and 0.3 mg/L inhibited shoot formation. Cultures stored for six months grew normally after being regenerated. The highest shoots and the highest number of leaves were obtained from the treatment of paclobutrazol without BA. This study indicated that the propagation media of aquatic plants Ludwigia sp. did not require high concentrations of BA. Cultures could be stored for over six months using paclobutrazol with 0.3-0.6 mg/L. 
Forest Structure and Tree Species Diversity of the Abasumba Globally Significant Biodiversity Area, Ghana Felix Tetteh Kabutey; Daniel Wodah
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76354

Abstract

We studied the forest structure and tree species diversity with diameter-at-breast-height (dbh) ≥ 10 cm in the Abasumba Globally Significant Biodiversity Area Ghana. Sixteen 25 m * 25 m plots were demarcated and trees with dbh ≥ 10 cm were inventoried following International Plant Nomenclature Index. The characteristic three–storey structure of tropical forests was shown, 68.7% of trees were in the lower 4.5–18 m and middle 18–30 m storeys. A majority 91.4% of 342 trees was in the dbh of 10–30 cm and a least 8.6% of 32 trees in 31–60 cm had dbh ˃ 60 cm. Total of 46 species, 38 genera and 17 families, with mean Alpha, Shannon and Simpson’s Diversity indices of 13.9, 1.44 and 0.07 and importance value index of 300.0 for 374 trees ha-1 was recorded. Plant families Sterculiaceae, Meliaceae, Leguminosae, Ulmaceae and Bombacaceae was the majority encountered while Triplochiton scleroxylon, Cola millenii, Trichilia monadelpha, Hymenostegia afzelii, Celtis mildbraedii, Ceiba pentandra and Ficus sur was the most occurring species in 54.0% of the plots accounting for 52.0% of the IVI for all trees. Blighia sapida, Bridelia grandis, Dialium guineense, Draceana arborea, Ficus sur, Holarrhena floribunda, Holoptelea grandis, Margaritaria discoidea, Rauvolfia vomitoria, Trilepisium madagascariense, Vitex ferruginea, Ximenia americana and Xylia evansii had one individual in the 10,000 m2 area indicated that they are rare and should be given conservation priority in the forest reserve. 
Alternative Strategy to Improve the Conservation of Javan Deer in Pangandaran Nature Reserve, West Java, Indonesia Fahmi Idris Firdaus; Rina Ratnasih Irwanto; Elham Sumarga
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76498

Abstract

The habitat of Javan deer in Pangandaran Nature Reserve (PNR) faced natural changes, particularly due to the succession process of vegetation community in grazing areas, and inadequate infrastructures that affected the deer to roam outside PNR. This study aimed to formulate strategies for the conservation of Javan deer in PNR, focusing on ecological aspects and conservation management. The methods were encountering Javan deer individuals; scan sampling and continuous recording to observe the behaviour of Javan deer; calculating the productivity of grazing area by defoliation experiment and vegetation analysis; reviewing documents, reports and interviews; and analysing strategy using SWOT-QSPM. Results showed there were 43 Javan deer encountered roaming in PNR and outside the conservation area, and nine individuals gathered in Cikamal grassland. The productivity of the grazing areas (5.61 ha) was 93,826 kg of feed annually and was only sufficient for 23 individuals. The grazing areas were dominated by Cynodon dactylon. Javan deer spent their time feeding. Javan deer herd in Cikamal is more intolerant to humans compared to the herd in Pangandaran Nature Tourist Park (PNTP). This study recommends: considering the management status of Javan deer in the conservation management of PNR and PNTP; improving the conservation management of Javan deer and its habitat; improving facilities and the management system of those facilities and conservation-supporting infrastructures; collaboration with researchers to perform some research and innovations for Javan deer conservation; improving the capability of PNR staff theoretically and practically; and educating and empowering the local people in terms of Javan deer conservation. 
Diversity of Fusarium Endophytes Isolated from Wild Bananas in Pandenglang, Indonesia Nani Maryani; Siti Yulianti; Indriati Ramadhani; Rida Oktorida Khastini; Suroso Mukti Leksono
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76897

Abstract

A group of Fusarium spp., in the Fusarium oxysporum species complex is known as pathogens on bananas, i.e., Fusarium wilt or Panama Disease. However, many Fusarium spp. are also known to be endophytes inside healthy banana plants and have been less explored and investigated. Fusarium endophytes have been demonstrated to be effective against the Fusarium pathogen that causes wilting in some crops such as tomatoes and watermelon. Thus, we explored endophytes Fusarium from local bananas in Pandenglang Banten for further use as biocontrol of Fusarium wilt. Four wild banana accessions were identified, from which 9 Fusarium isolates recovered from its pseudostems asymptomatic plants. All isolates were characterized based on their morphological characters and sequence of the Internal Transcribed Spacer (ITS) gene. These isolates belong to four complexes of Fusarium i.e. Fusarium equiseti species complex, Fusarium oxysporum species complex, Fusarium sambucinum species complex, and Fusarium solani species complex (currently described as Neocosmospora). Further study on molecular characterization of these isolates using specific genes and their potential antagonists of pathogens still needs to be discovered for other use as a biocontrol against Fusarium wilt.
Genetic Variation of Butternut Squash (Cucurbita moschata Duchesne) based on Inter-Simple Sequence Repeat Nabila Rizki Ananda Putri; Prima Sekti Kusnanda; Bunga Finovel Angellya; Dian Sartika; Purnomo Purnomo; Budi Setiadi Daryono
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.77228

Abstract

Butternut squash (Cucurbita moschata) is a Cucurbitaceae plant that has been widely cultivated in Indonesia. Butternut squash is known to have various cultivars. A new cultivar introduced by the Faculty of Biology UGM is named ‘Citra Laga’ which is expected to be able to compete with the imported cultivars. The number of cultivars within a species may indicate genetic variation. This research was conducted to observe genetic variation and the phenetic relationship between ‘Citra Laga’ and the imported butternut squash cultivars based on the molecular marker ISSR. The ISSR analysis between ‘Citra Laga’ and the imported cultivars showed an average low polymorphism rate by 18.61% with a high similarity percentage of 83.7%. Thus, it can be said that the genetic variation is low and ‘Citra Laga’ is not genetically much different from the imported cultivars. 
Diversity of Butterflies in Ledokombo Hillocks Jember, East Java, Indonesia Husni Mubarok; Alfina Eka Dwi Candra; Bayu Sandika; Wiwin Maisyaroh; Ellena Yusti; Agmal Qodri
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 1 (2023): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.77655

Abstract

Ledokombo hillocks are small hills located in Jember, East Java that have natural resources and face habitat alteration such as plantation and mining.  However, a study of the diversity of butterflies has not been carried out in this area. We analysed the diversity of butterflies using the Shannon-Wiener diversity index (H’) and Pielou evenness index (E).  We identified 514 individuals from 34 species and demonstrated a moderate diversity of butterflies (H'= 1.907) in this area. Our study results could be used for sustainable ecological management of plantations in Ledokombo Hillocks, Jember, East Java. 
Diversity and Community Structure of Dragonflies (Odonata) in Various Types of Habitat at Lakarsantri District, Surabaya, Indonesia Muhamad Azmi Dwi Susanto; Nirmala Fitria Firdhausi; Saiful Bahri
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.76690

Abstract

Dragonflies are insects that are very dependent on the existence of freshwater ecosystems, However, the population of dragonflies in urban freshwater ecosystems is at risk due to a number of issues. Consequently, it is essential to carry out research and efforts to preserve dragonflies in urban areas. This study aims to provide information about the diversity and structure of dragonfly communities in various habitat types in the Lakarsantri, Surabaya. Data collection in this study was carried out in the habitat types of ponds, reservoir, river, and rice field in July to September 2021. The study results show there are 22 species from 4 families with a total of 827 individuals. Analysis of the Shannon-Wiener diversity index showed that the highest value of dragonfly diversity was found in a pond, with a value of H' = 2.40, and the location with the lowest value was a river, with a value of H' = 1.77. At four research locations that have different aquatic ecosystems, the community structure of dragonfly is also different. The composition of the dragonfly community structure at the reservoir location has similarities to a pond, and at a river location, it has similarities to a paddy field. Differences in abiotic factors consisting of light intensity, humidity, and temperature at each study location have a correlation with differences in dragonfly community structure. In addition, the composition of the vegetation at each location is also one of the factors causing differences in the structure of the dragonfly community. 
In Silico and Validation Approaches for Optimum Conditions of Rattus norvegicus Target Gene qPCR Primers Gracia Alice Victoria Pollo; Nyoman Yudi Antara; Firman Alamsyah; Rarastoeti Pratiwi
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.71765

Abstract

The qPCR method requires an oligonucleotide pair to prime the amplification process. With the variety of qPCR reagent and primer options available, in silico and laboratory experimental validation approach was needed to validate the most suitable primer for prior use. This article aims to provide in silico analysis of actin alpha-2 smooth muscle (Acta2), fibroblast activation protein (Fap), hypoxanthine phosphoribosyltransferase-1 (Hprt1), platelet-derived growth factor subunit B (Pdgfb), phosphoinositide-3-kinase regulatory subunit-1 (Pik3r1), and vascular cell adhesion molecule-1 (Vcam1) qPCR primer with qPCR and electrophoresis validation. The procedure used in this approach was in silico analysis of primer from published articles and newly designed primer. The analysis was done with Primer-BLAST for gene specificity, Primer-Dimer, OligoCalc for hairpin formation, BLAST Nucleotide for identical sequence screening, and Clustal Omega for product length validation. Experimental validation was done using qPCR for optimal annealing temperature, priming ability, and amplificon specificity, and electrophoresis for product length validation. This assessment resulted in in silico and laboratory experimental validation of Acta2, Fap, Hprt1, Pdgfb, Pik3r1, and Vcam1 primer pairs producing suitable amplicon for qPCR using Rattus norvegicus cDNA with SYBR annealing temperature range of 60-65°C with three mM MgCl2. The primer pair can be used for further qPCR analysis under similar conditions and the procedure stated can be used as starting point for qPCR Primer preparation.
Essential Oils of Etlingera acanthodes A.D. Poulsen, An Endemic Ginger from Sulawesi Island Hurria Hurria; Anggra Alfian; Muhammad Fajri Ramadhan Muslim Saleh; Heder Djamaludin; Murni Mursyid; Witno Witno; Asri Subkhan Mahulette
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 2 (2023): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.72117

Abstract

Etlingera acanthodes A.D. Poulsen is a ginger endemic to Sulawesi, and there is no research on its essential oils. The Essential oil information of E. acanthodes is the first reported and has a high novelty. The objective of the study is to analyze the component of E. acanthodes essential oils, which are endemic to Sulawesi. The essential oils obtained by hydrodistillation of the leaves, stems, flowers, and rhizomes of E. acanthodes A.D. Poulsen. The samples were analyzed by GC-MS method using the Agilent Technologies 7890 Gas Chromatograph with Auto Sampler. Data analysis of essential oils of GC-MS results were determined based on comparing mass spectra from the NIST 2005 v.2.0 library and Wiley 7 library 2003. The oils of E. acanthodes contain terpenoids, phenolic, steroids, and other compounds. Phenolic compounds (39.56%) predominate in leaves, other compounds (41.25%) on stems, diterpenes hydrocarbons (77.3%) in flowers, and other compounds (43.5%) and steroids (40.4%) on rhizomes. The five main compounds of E. acanthodes are Neophytadiene; (+)-De-O-Methylcentrolobine; Cholest-5-En-3-Ol,23-Ethyl-,(3. Beta.23S)-; 9,12-Octadecadienoic Acid, Phenol, 2-ethyl-. The analysis found several compounds that can be used for industry and medicine in the future.  

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