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INDONESIA
Journal of Tropical Biodiversity and Biotechnology
Published by Universitas Gadjah Mada
ISSN : 25409573     EISSN : 25409581     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
Arjuna Subject : -
Articles 327 Documents
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Ulva lactuca Linnaeus Potentially Promotes Reproductive Indices and Depressive-like Behavior of Hypertriglyceridemia Male Wistar Rats (Rattus norvegicus Berkenhout, 1769) Anggoro Chandra Yulistiyanto; Mayumi Hersasanti; Rahadian Yudo Hartantyo; Laksmindra Fitria; Abdul Razaq Chasani; Mulyati Mulyati
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.57924

Abstract

Excessive consumption of fatty foods can lead to hyperlipidemia, which is often coupled with hypertriglyceridemia (HTG), a condition where blood plasma triglyceride (TG) levels elevated beyond normal levels. This condition may disturb physiological functions of the body, such as reproductive functions, and other physiological imbalances leading to chronic stress and depression. Ulva lactuca is a potential natural treatment for HTG, as it contains various nutrients to aid physiological functions. This seaweed also has high levels of Cd, which can increase depression. Therefore, research on the potential benefits of U. lactuca should be followed by an investigation of its health risks. This research aimed to examine the effects of HTG and treatment with U. lactuca on reproduction and depressive-like behavior of male Wistar rats (Rattus norvegicus Berkenhout, 1769). The data collected in this research include body weight, serum TG concentration, gonadosomatic index (GSI), serum testosterone concentration using competitive ELISA, and depressive-like behaviors assessed using the Forced Swim Test (FST) and Open Field Test (OFT). Data were analyzed using One-Way ANOVA followed by DMRT, independent- and paired-samples t-test, and Kruskal-Wallis H test with a significance value of α=0.05. Body weight, serum TG and testosterone concentration, GSI, and depressive-like behaviors were increased by the HTG condition. Ulva lactuca at the1500 mg/kg BW/day did not significantly affect body weight, testosterone concentration, and depressive-like behaviors of HTG rats. Meanwhile, this treatment significantly increased the GSI and depressive-like behaviors of healthy rats. These results suggest that Ulva lactuca treatment not only enhances gonad growth and development but also increases depressive-like behaviors.
Avifaunal Diversity and Community Structure in Universitas Brawijaya Forest, East Java, Indonesia Agus Nurrofik; Muhammad Fathoni; Agung Sih Kurnianto; Luhur Septiadi; Nia Kurniawan
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.58335

Abstract

Avifauna inhabiting the mountainous forest ecosystem is severely threatened by anthropogenic disturbances, especially in the Java island of ­­Indonesia. Yet, efforts to monitor the avifauna diversity are lacking, including in one of the mountainous forest areas, Universitas Brawijaya Forest (UBF). In this study, information about diversity, community structure, feeding specialization, and conservation status of avifauna is presented. Observations were conducted from December 2019 to February 2020 on two designated tracks with different degree of disturbances. Data were analyzed based on their conservation status, local distribution, feeding specialization (Jaccard similarity index), species richness, total abundance, species diversity (Shannon-Wiener diversity index), and importance value index (IVI). A total of 51 species from 27 families were identified. Two species at risk (one Near Threatened and one Endangered) and 9 protected avifauna were noted. This study can be used as the baseline data for future conservation management in the UBF.
Antibacterial Activity of Kecombrang Flower (Etlingera elatior (Jack) R.M. Sm) Extract against Staphylococcus epidermidis and Propionibacterium acnes Vilya Syafriana; Renita Noviani Purba; Yayah Siti Djuhariah
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.58528

Abstract

This study aimed to determine the antibacterial activity from the ethanol extract of Kecombrang flower (Etlingera elatior (Jack) R.M. Smith) against Staphylococcus epidermidis and Propionibacterium acnes. The extract was made by the maceration method with 70% ethanol as a solvent. Antibacterial activity test was carried out by the disk diffusion method with a concentration of 10%, 20%, 40%, and80%. Meanwhile, theMinimum InhibitoryConcentration(MIC)was done atconcentrations of 10%, 8%, 6%, 4%, and 2%. The results showed that the Kecombrang flower (Etlingera elatior (Jack) R.M.Smith) extract hadantibacterialactivityagainstS.epidermidisand P. acnes. The MIC for S. epidermidis is at a concentration of 4%, while in P.acnes cannot determine yet.
Predicting the Distribution of Sunda Pangolin (Manis javanica Desmarest, 1822) in Way Canguk Research Station, Bukit Barisan Selatan National Park, Lampung Silvi Dwi Anasari; Wulan Pusparini; Noviar Andayani
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.58612

Abstract

The distribution of a species can help guide the protection activities in their natural habitat. Conversely, the lack of information on this distribution makes the protection strategy of this species difficult. The research was conducted in Way Canguk Research Station, Bukit Barisan Selatan National Park from January until March 2018. The purposes of this research were to create a distribution prediction map of Sunda pangolin (Manis javanica) and estimating the environment variables that most influenced the probability of the distribution. Fourteen points of camera trap coordinates were used for presence data with nine types of environment variables such as elevation, slope, understorey, canopy cover, distance from roads, distance from rivers, distance from villages, food source, and distance from the threat. The result of maxent showed an Area Under the Curve (AUC) value of 0.909 categorized as very good. The highest probability of Sunda pangolin distributions was in the Pemerihan Resort and Way Haru Resort area, while the dominant environmental variables included the distance from the village, the canopy cover, and the distance from threat with the value 47.7; 25.85; and 15.8%, respectively. Prediction maps and environment variables can help to identify the population of Sunda pangolin in the wild and can provide input for the national parks to prioritize protection areas for Sunda pangolin from the increased poaching.
The Use of DNA Barcoding and Phylogenetic Analysis to Improve Identification of Usnea spp. Based on ITS rDNA Miftahul Jannah; Muhammad Rifqi Hariri; Rina Sri Kasiamdari; Niken Satuti Nur Handayani
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.58635

Abstract

Lichen of the genus Usnea is quite common being used as a traditional herbal remedy. This genus is characterized by thallus, which is very similar among the species, leads to some difficulties in distinguishing them. In Indonesia, such research report on the availability of this genus based on their morphological characteristic is minimal. This might be due to too high morphological similarities among them. The molecular character, which is based on the DNA Barcode of Internal Transcribed Spacer (ITS) rDNA sequences, with its conserved region (5.8S) and varied region (ITS1 and ITS2), are becoming essential characters on identifying as well as analyzing the phylogenetic. The current study then proposed to identify and draw the species dendrogram of species within the Usnea genus obtained from Mount Lawu Forest of Central Java and Turgo Forest of Yogyakarta based on their phylogenetic and phenetic analysis. The dendrogram was constructed with UPGMA using the simple matching coefficient, whereas the phylogenetic tree was constructed with Maximum Likelihood (ML) using Kimura-2 parameter with 1000 bootstrap. The data were unable to draw phenetic relationships among the subgenus Usnea and Eumitria members. The phylogenetic tree shows the primary two clades, distinguishing the subgenus Usnea and Eumitria. The ITS rDNA sequence was able to identify most of the Usnea species.
Stable Transformant of Phalaenopsis amabilis Somatic Embryo Carrying 35S::AtRKD4 Develops Into Normal Phenotype of Transgenic Plant Naufal Ghozi Aditya Perdana; Windi Mose; Muhammad Dylan Lawrie; Jose Gutierrez-Marcos; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 2 (2021): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59210

Abstract

Phalaenopsis amabilis (L.) Blume orchid is an Indonesian national flower. The number of these orchids in their natural habitat is very limited, therefore plant propagation efforts are needed. One of the promising methods is plant propagation by inserting embryo gene AtRKD4 from a model plant Arabidopsis thaliana into the orchid genome to produce many somatic embryos. From previous research, we have obtained 28 plant P. amabilis transformants carrying the AtRKD4 gene, however, it was unknown whether these plants have normal phenotypes and growth similar to their parents. Therefore, descriptions on growth and morphology are needed. This research aimed to evaluate the phenotype of P. amabilis carrying 35S::AtRKD4 the transformants grown in greenhouse. To achieve it, AtRKD4 gene integration stability on transformants genome was analyzed. Morphology and cross-section anatomy structure on transformant and non-transformant plantswere described. The stability of AtRKD4 gene integration in the plant genome was confirmed by amplification of the AtRKD4 gene from genomic DNA with Polymerase Chain Reaction (PCR) using a specific primer for AtRKD4 and ACTIN genes as the internal control. The quantitative data from morphology and anatomy measurements were analyzed statistically using ANOVA. The results showed that AtRKD4 was stably integrated into the genome of P. amabilis transformants and all transformant plants showed similar morphology and anatomy characteristics as non-transformant plants. The AtRKD4 embryo gene was stably integrated into the orchid genome and the transformant plants grow normally without significant changes in phenotype.
Epilithic Microalgae Isolated from Biofilm on Borobudur Temple Stone Debora Christin Purbani; Ade Lia Putri; Moh. Habibi
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59216

Abstract

Borobudur Temple is a historical heritage building located in an open area and made of porous building materials (stone materials). This condition makes the Borobudur Temple susceptible to various problems related to degradation and weathering. Biodeterioration of Borobudur Temple may be caused by activities of living organisms present in the biofilm of stone. Continuous monitoring and evaluation need to be carried out by observing and isolating the growth of micro-organisms, including epilithic microalgae. Therefore, this study aims to isolate and identify epilithic microalgae from the biofilm on Borobudur Temple stones. Epilithic microalgae were isolated to obtain a uni-algae and maintained under culture conditions. The morphological of microalgae were observed using light microscopy, while the 18S rRNA gene sequence determined the molecular identification of microalgae for eukaryotic and 16S rRNA sequence for prokaryotic. A total of nine epilithic microalgae were successfully isolated from the biofilm of Borobudur Temple stones. The isolated were identified as Ankistrodesmus falcatus, Tetraselmis cordiformis, Pseudendoclonium arthropyreniae,  Anabaena cylindrica,  Nostoc gelatinosum, Oscillatoria limnetica, Messastrum gracile, Stigeoclonium aestivale, and Scenedesmus acuminatus. This is the first study for the identification of microalgae from Borobudur temple stones. The isolates will be collected and will be used as a source for further study.
Biomass Estimation of Eaglewood (Aquilaria filaria (Oken) Merr.) in the Karst Ecosystem of West Papua Andes Hamuraby Rozak; Zaenal Mutaqien; Destri Destri
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59221

Abstract

Eaglewood is Indonesia’s important trade commodity in the form of resins from several infected species of Thymelaeaceae. The basis to determine its international trade quota through CITES is derived from the estimated eaglewood-producing species grown in their habitat. This paper aims to estimate the biomass of eaglewood, Aquilaria filaria, in the karst ecosystem of West Papua. We conducted a plot-based method and calculated the biomass of A. filaria using a diameter-based allometric equation and simulated using a bootstrap procedure. The results showed that 15,500 tons of naturally infected eaglewood are estimated in the karst ecosystem of West Papua.
Update on New Species and Record of Fishes in the Coral Triangle Region for the Last 10 Years (2008-2019) Fione Yukita Yalindua; Teguh Peristiwady; Putri Saphira Ibrahim
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59230

Abstract

Updated data is an essential requirement for carrying out research, planning, and policy briefs. The Coral reef triangle region is one of the areas with the highest diversity of marine biota and the discovery of new species in this area are increasing every year, much of this information is already available. However, most of the data is not available per region and is still scattered. This study aims to create a checklist and assessment of new species and a new record of fishes from this region over the last ten years based on several aspects, including species composition, pattern of distribution, endemicity, and depth using every source of the report and secondary literature data. The current new species and a new record of fish in the last decades combined consists of 360 species (268 new species and 92 new records). The most speciose group of family dominated by Gobiidae (93), followed by Labridae, Pomacentridae and Serranidae (18), Apogonidae (17), Dasyatidae (15), and the rest were ranged from 1-9 species per family. More than half of new species and new records are found in Indonesia, followed by the Philippines, Papua New Guinea, and Solomon Island. The result shows that cryptobenthic Families especially Gobiidae from genus Trimma and eviota are dominated the trend of new species and new record discovery and it is expected to rise over time while there will also be an emergence of some possibly new endemic species from major and rare families from the eastern part of Indonesia (West Papua and Papua New Guinea). Thus, the eastern part of Indonesia (Papua, Maluku, Aru Sea, and Papua New Guinea) and the northern part of Indonesia (North Sulawesi and Philippine) are suitable for exploration for marine biodiversity discovery research in the future.
Callus induction and secondary metabolite profile from Elephantopus scaber L. Junairiah Junairiah; Diah Ayu Wulandari; Edy Setiti Wida Utami; Nabilah Istighfari Zuraidassanaaz
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59234

Abstract

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol.  Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.

Page 9 of 33 | Total Record : 327

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