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INDONESIA
Journal of Tropical Biodiversity and Biotechnology
Published by Universitas Gadjah Mada
ISSN : 25409573     EISSN : 25409581     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional biology, and biotechnology.
Arjuna Subject : -
Articles 327 Documents
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Diversity and Distribution of Herpetofauna in Banyu Nibo Waterfall Ecotourism Region, Nglanggeran, Gunung Kidul, Special Region of Yogyakarta Alfonsus Toribio Eko Saputro; Elika Boscha; Ananto Puradi Nainggolan; Donan Satria Yudha; Rury Eprilurahman
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 1 (2020): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53637

Abstract

Banyu Nibo Waterfall is located near Nglanggeran, Gunung Kidul that well known for its eco-tourism development. The geographical and ecosystem condition could be providing unique habitat for herpetofauna. Herpetofauna has important role in the ecosystem as food chain components and even as environment bioindicator.The aim of this research is to asses the herpetofauna diversity in Banyu Nibo Waterfall as preliminary biodiversity data.  The research was conducted using VES (Visual Encounter Survey) assisted by 500 meters transect line. As a result, 15 species were found, mainly distributed on area with tree coverage.
RAPD Analysis for Genetic Variability Detection of Mutant Soybean (Glycine max (L.) Merr) Didik Wahyudi; Lia Hapsari; S. Sundari
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 1 (2020): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53653

Abstract

This study aimed to detect and evaluate the genetic mutation from mutagenized soybean by RAPD markers. Soybean seeds of “Grobogan” variety were treated with two different concentrations of EMS (0.5% and 1%) and three incubation times (4, 6 and 8 h). DNA whole-genome was isolated from young leaf seedling with the Qiagen DNeasy Plant Mini Kit. Twenty OPA primers (OPA-1 to OPA-20) were used for DNA amplification. The results showed that EMS treatments successfully generated genetic variation in soybean, which indicated by high values of PIC, EMR, and MI. RAPD primers that effective to detect the mutation were OPA-2, OPA-07, OPA-10, OPA-11, OPA-12, OPA-13, OPA-14, OPA-15, OPA-16, OPA-18 and OPA-20. The band expression of those primers was exhibited a stronger intensity along with increasing of EMS concentration and incubation time used in this study. Treatment of 0.5% EMS in 6 hours incubation was successfully generated soybean mutants with the lowest genetic similarity compared to the wild-type. Thus, this study provides a new approach to generate genetic variability in soybean and has the potential to improve for soybean breeding program.
Estimation of Above Ground Carbon Sequestration in Trembesi (Albizia saman) and Johar (Senna siamea) at PT Multi Harapan Utama, East Kalimantan Widya Fajariani; Medi Hendra; Dwi Susanto
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.43381

Abstract

The open-pit mining method has a very large ecological impact. It causes the loss of forest vegetation which decreases CO2 absorption. Measuring the amount of carbon stored in plant biomass can represent the amount of CO2 that can be absorbed in the atmosphere. The objective of this research is to determine the carbon sequestration of Albizia saman and Senna siamea in different age classes at PT MHU Busang Jonggon Block, Kukar, East Kalimantan. Estimation of carbon sequestration in the stands of A. saman and S. siamea was carried out by non-destructive methods using biomass allometric equations while in understorey and litter using the destruction sampling. The results showed that the highest carbon absorption value of A. saman was 314.28 tons/ha which appear at six years old stands and the lowest value was 193.31 tons/ha at three years old stands while the highest carbon absorption value of S. siamea was 113.65 tons/ha which appear at nine years old stands and the lowest value was 24.64 tons/ha at three years old stands. A. saman could be more promising plant species than S. siamea according to its higher level of carbon sequestration and their high adaptation level. All data from this study could suggest several information for increasing carbon sequestration level in forest ecosystem as well as achieving forest rehabilitation purpose.
The Diversity of Ray-finned Fishes (Actinopterygii) in Plio-Pleistocene Java Donan Satria Yudha; Muhammad Ageng Prabowo; Rusyad Adi Suriyanto; Didit Hadi Barianto
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.43694

Abstract

Java has been known in the world of Paleontology as a contributor to the findings of Homo erectus fossils, but there are still other fossil findings that have not been identified until now, especially fossil fishes of the subclass Actinopterygii. This research was conducted to recognize the diversity of the actinopterygians fishes in Plio-Pleistocene of Java and to determine the diagnostic characters of each taxon group of fossils in the Plio-Pleistocene of Java. The study was carried out using comparative anatomical methods with present-day specimens and fossil findings collection of the Laboratory of Bioanthropology and Paleoanthropology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada; Bandung Geological Museum and Sangiran Early Man Site. The research found at least 8 species of fish fossils in Java which belong to three order, i.e., the order Siluriformes with 5 identified species: Bagarius gigas, Hemibragus nemurus, Clarias macrocephalus, family Ariidae with indeterminate genus or species, Plotosus canius, Clarias batrachus, and family Pangasiidae with indeterminate genus or species; the order Perciformes with two identified species: Anabas testudineus and Sphyraena crassidens; and the order Cypriniformes with one identified species: Osteochilus vittatus. Based on the fossil findings showed that the Java Island during the Plio-Pleistocene used to be a marine environment that gradually ascending into a lowland river which closes to mangrove swamps and estuaries while the ancient Bandung lake site was a lacustrine environment with calm currents and is overgrown with riparian vegetation.
Innovation of Natural Orchid Cultivation Technology for Tourism Development in Banyunganti Hamlet, Jatimulyo Village, Girimulyo Sub-District, Kulon Progo District, Yogyakarta Endang Semiarti; Aziz Purwantoro; Ari Indrianto; Aries Bagus Sasongko; Oktaviana Herawati; Asri Fajar Milasari
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.46283

Abstract

Orchid is the best tourism icon which focused on nature-based tourism development in Indonesia. Banyunganti Hamlet is one of the tourism villages in Kulon Progo which has a high diversity of orchid species. Regarding this situation, guiding and assistance for villagers which is focused on the introduction of natural orchid species and its character, conventional propagation, and modern propagation by using household-scale tissue culture techniques (sowing seeds and planting) is important to give. The long-term goal for this activity was for empowering women in Dusun Banyunganti in line with the opening of the New Yogyakarta International Airport by the government.
The Effectiveness of Red Spinach (Amaranthus tricolor L.) and Green Spinach (Amaranthus hybridus L.) Extracts for Bacillus thuringiensis var. kurstaki Protectant against UVB Radiation for the Control of Armyworm (Spodoptera litura Fab.) Siti Sumarmi; Mifta Arlinda; Sukirno Sukirno
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53004

Abstract

Spodoptera litura Fab. is an insect that damage cultivated plants in Indonesia. Efforts to control it can be done by using biological agents for example by using Bacillus thuringiensis kurstaki (Btk.). Unfortunately, the Btk. is easily degraded by UV radiation. This research aimed to study the effectiveness of red and green spinach as UVB protection for Btk. and to observe the pathogenicity of Btk. formulations against armyworm. Furthermore, the sublethal effect of Btk. against S. litura was investigated. The morphology of the endospore, protein crystal, and bacterium were observed under a contrast phase microscope. The extracts at 2% (w/v) were mixed with Btk. suspensions at 5 x 104, 5x105, and 5x106 (spores/ml), respectively. The formulations then exposed under Ultraviolet B (UVB) lights for 3, 6, and 9 hours then tested against the 3rd larval instar of armyworm. The larval mortality was observed daily and the analysis of variance was analyzed by one way anova. The sublethal effects of the treatment to the pupal and adult stages were observed when the moths emerge. The results showed that the larval mortality caused by  Btk. mixed with red spinach ranged from 11.7 to 26.7%. The sublethal effects of Btk. resulted in smaller sizes of pupae and imago, darker pupae, and wings abnormality of the adult stage, compared to any control treatment. The morphological observation of the bacteria showed that extracts gave UV protection against UVB. These results suggested that red and green spinach potentially can be used as a protectant for Btk. against UVB.
Species Diversity and Potential Utilization of Moraceae in Nglanggeran Ancient Volcano, Gunungkidul Regency, Yogyakarta Reinatawas Febri Santika; Ratna Susandarini
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53005

Abstract

Nglanggeran Ancient Volcano is one of the ecotourism areas in Gunungkidul Regency, Yogyakarta. This ancient volcano is one of the geological sites in Pegunungan Sewu. Pegunungan Sewu is a National Geopark in Indonesia and has been designated as a UNESCO Global Geopark since 2015. The determination of an area into a National Geopark and Global Geopark makes the biodiversity in the area must be protected and preserved, including the plant diversity, one of which is Moraceae. The ecological and economic importance of Moraceae in Nglanggeran Ancient Volcano has not been documented properly. This fact encourages the need to do this research. The aim of this research was to record species diversity of Moraceae and its potential uses. The research was conducted in August-November 2019 at Nglanggeran Ancient Volcano and followed by data analysis in Plant Systematic Laboratory, Faculty of Biology, Universitas Gadjah Mada. The samples were collected at two different tracks, covering the track to the peak and the pathway down. There were six species found, namely Artocarpus altilis (Parkinson) Fosberg, Artocarpus heterophyllus Lam., Ficus benjamina L., Ficus septica Burm. f., Maclura cochinchinensis (Lour.) Corner, and Streblus taxoides (Roth) Kurz. The potential uses of Moraceae by local people were identified from the interview and enriched by data from literature studies indicated that Moraceae plants were used as a food, medicine, for construction, soil protection plants, and houseplant.
Induction of Microspore Embryogenesis of Eggplant (Solanum melongena L.) ‘Gelatik’ Devi Bunga Pagalla; Ari Indrianto; Maryani Maryani; Endang Semiarti
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53677

Abstract

The haploid or double haploid plant of eggplants could be produced from microspore culture (embryogenesis of microspores). In the breeding programs, microspore can be developed into an embryo directly after exposure to stress treatment during cultured. Stress (temperature and starvation medium) is an important factor in the induction of embryogenesis microspore. This study aims to induced embryogenic microspores from eggplant CV. Gelatik. The stage late-uninucleate microspore (Vacuolate Microspore/VM) and early binucleate (Young Bicellular Pollen/YBP) are the suitable stages to induce multinucleate structure. There are 3 methods used in this research; 1) Determination of the stage development of microspore based on flower buds length and anther length. 2) Induction of embryogenic microspore on the pre-treatment and starvation medium. 3) After giving pre-treatment for 4 days, micropores were transferred to culture medium A2 at 28oC in dark conditions to induce the multicellular structures. This study reported that 50-68.51% of the VM+YBP stage obtained in the range of flower bud lengths of 10-17 mm, and 5.0-6.9 mm, the range of anther length containing VM+YBP of 50-77.48%. The pre-treatment heat shock at 33oC in the medium B for 2 days,  produced embryogenic microspores with a high percentage, that is about 50.19%, while microspores at 25oC and 4oC respectively 46.17% and 49.28%. Pre-treatment for 4 days at 4 oC, 25 oC,  and 33oC with the percentage of embryogenic microspores apiece 32.87%, 27.45%, and 37.34%. The multicellular (starlike) structure begins forming on the fifth day of incubation in culture medium (A2) after pre-treatment in B medium at 33oC.
Ectoparasite Infestation among Stray Cats around Surabaya Traditional Market, Indonesia Shifa Fauziyah; Abdul Hadi Furqoni; Norma Farizah Fahmi; Adi Pranoto; Pradika Gita Baskara; Lensa Rosdiana Safitri; Zukhaila Salma
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 3 (2020): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53687

Abstract

This study was conducted to determine the prevalence of ectoparasite infestation among stray cats around Surabaya traditional markets. A total of 305 stray cats were collected around 17 traditional markets in Surabaya City and were examined for the presence of fleas with a fine-toothed flea comb. Surveys were conducted during May-June 2019. 228 of 305 stray cats (74.75%) were infested with one species of ectoparasite. The average number of C. felis in every cat was 2.54, while the number of F. subrostratus in every cat was 0.33. Additional data about the gender, pregnancy/maternity, and bodyweight of every cat were recorded. The result of chi-square test shows that there is a significant difference between gender, pregnancy status, and bodyweight by the occurrence of ectoparasites (p=0.008; p=0.00; p=0.00). A total of 878 ectoparasites consisting of flea and lices, namely Ctenocephalides felis (88.27%) as the dominant ectoparasite, followed by Felicola subrostratus (11.73%). The highest infection rate (prevalence) of ectoparasite was found in Pucang Market (16.81%), while the lowest prevalence was found in Mulyorejo Market (0.8%). Coinfection was observed in only a few cats (1.63%). Multiple Regression showed that pregnancy is the most influential factor in the occurrence of fleas (p=0.000). These results should be taken into account among health workers to prevent a possible outbreak of zoonotic diseases caused by fleas. 
Physicochemical Characters of Mosquitoes Natural Breeding Habitats: First Record in High Dengue Hemorrhagic Fever Cases Area, East Java, Indonesia Rosmanida Rosmanida; Shifa Fauziyah; Adi Pranoto Pranoto
Journal of Tropical Biodiversity and Biotechnology Vol 5, No 2 (2020): August
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.53714

Abstract

This research aims to identify physicochemical characteristics in natural breeding habitats/ phytotelmata of dengue vector—including Aedes (Stegomyia) aegypti and Aedes (Stegomyia) albopictus. The research was conducted during rainy season and pre-dry season (from January to June 2017) in the region with the high cases of Dengue Hemorrhagic Fever (DHF). The entomological survey was carried out by stratified random sampling in urban and rural areas in order to find potential breeding habitats, every natural breeding habitats in sampling location were checked for the presence of Aedes larvae. Physicochemical characters that consist of temperature, turbidity, carbon dioxide, ammonia, nitrate, sulphate, pH and dissolved oxygen were recorded. Larval species were taken and then identifying activities were conducted in the Laboratory of Entomology. Data were analysed using the Chi-square test. Results showed that only dissolved oxygen that significantly associated with larval abundance (p=0.039). while others are not significantly associated. Whereas, other characters are associated with each other, carbon dioxide associated with the ammonia and sulphate (p=0.001; p=0.028). Turbidity associated with the dissolved oxygen (p=0.022) and pH associated with nitrate (p=0.001). 

Page 7 of 33 | Total Record : 327

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