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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 15 Documents
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Search results for , issue "Vol 8, No 1 (2018)" : 15 Documents clear
Bioactivity of Antibacterial Compounds Produced by Endophytic Actinomycetes from Neesia altissima Pratiwi, Rina Hidayati; Hanafi, Muhammad; Artanti, Nina; Pratiwi, Rosa Dewi
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (835.918 KB) | DOI: 10.11594/jtls.08.01.07

Abstract

Identification of alternate bioactive compounds isolated from microbial endophytes from plants may lead to better solution against antibiotic resistance. Main objective of this research is to determine the bioactivity of antibacterial compounds produced by endophytic actinomycetes, which was isolated from stem bark of Neesia altissima Bl. at Halimun Salak Mount. Detection of bioactive compounds was assayed using Thin Layer Chromatography (TLC) by employing bioautography. Bioactivity was assayed using crude extract against five microorganisms using agar diffusion methods. Tetracycline was used as a positive control. The result of antibacterial activity from endophytic actinomycetes isolate revealed that the bioactive compounds were effective in inhibiting the growth of bacteria Bacillus cereus (1.25 mm), Salmonella typhimurium (5.75 mm), and Shigella flexneri (4.37 mm). Bioactivity of ethyl acetate extract from bioactive compounds of endophytic actinomycetes has an antibacterial activity against bacteria Bacillus cereus (13.00 mm) and Staphylococcus aureus (15.90 mm), at a concentration of 250,000 ppm. Each of TLC fraction was able to inhibit growth of B. cereus at a minimum concentration of 10,000 ppm. In conclusion, the bioactive compounds produced by endophytic actinomycetes can have inhibitory effect towards B. cereus. The category of that compounds are narrow spectrum. The bioactive compound isolated from endophytic actinomycetes may have other implications such as anticancer, antifungal, and antioxidant.
THE POTENTIAL OF INDIGENOUS BACTERIA FOR REMOVING CADMIUM FROM INDUSTRIAL WASTEWATER IN LAWANG, EAST JAVA Pambudiono, Agung; Suarsini, Endang; Amin, Mohamad
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (688.873 KB) | DOI: 10.11594/jtls.08.01.11

Abstract

Heavy metals have been used in various areas around the world especially in the industrial sector. Heavy metals contamination is very dangerous for ecosystem because of its toxicity for some organisms. Cadmium (Cd) is a dangerous metal pollutant that can cause remarkable diverse of toxic effects, in particular for humans and animals. The use of bacteria as bioremediation agents has been widely studied because more efficient, less cost, and environmentally friendly strategy. This present study aimed to isolate and identify Cd-resistant bacteria from the industrial disposal site. Wastewater samples were collected from disposal site of agar flour industry in Lawang Malang, East Java. The collected wastewater effluent was analyzed for physicochemical properties. Isolation of Cd-resistant bacteria was carried out using serial dilution. Bacterial isolates were observed and tested for their effects on the content of Cd. The content of Cd was tested daily using Atomic Absorption Spectroscopy (AAS) for seven consecutive days. Data was analyzed using one-way ANOVA (p < 0.05) and Tukey test. Characterization of potential bacterium was performed using bacterial identification kit. Four bacteria isolates have been successfully isolated from the wastewater sample. There was a statistically significant difference between groups as determined by one-way ANOVA (F = 1229.62, p = 0.00). A Tukey post hoc test revealed that all conditions are significantly different from each other. The content of Cd in wastewater sample was statistically significantly lower after taking the A isolate (3.39 mg/L, p = 0.00), B Isolate (1.47 mg/L, p = 0.00), C Isolate (1.15 mg/L, p = 0.00), and D isolate (1.95 mg/L, p = 0.00) compared to the control treatment (5.11 mg/L, p = 0.00). Two of the most potential isolates identified as Pseudomonas flourescens (C isolate) and Enterobacter agglomerans (B isolate).
Toxicity Test on Wedusan (Ageratum spp.) Extract Against Sitophilus spp. (Coleoptera: Curculionidae) Mosip, Erinus; Rahardjo, Bambang; Astuti, Ludji
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.525 KB)

Abstract

Tropical condition in Southeast Asia has contributed around 30% of post harvest damage as it provide optimum condition for the growth of Sitophilus spp. pest. Therefore, this study was aimed to investigate the effect of wedusan extract on controlling Sitophilus spp. Growth. Wedusan extract was given in different level of concentrations. The research used the Randomized Complete Design. Wedusan (Ageratum spp.) extract affected the imago mortality of Sitophilus spp. There were two kinds of Wedusan extract used in this study, dried extract (DE) and fresh extract (FE).  Sitophilus imago were put into bottles filled with organic rice and were treated with Wedusan extract. The mortality level was obsered at 24 Hours After Application (HAA), 48 HAA, and 72 HAA. The highest toxicity was found by the treatment of dried extract of wedusan 5 tablets, by LC50 55% on 24 HAA and 82.25% on 72 HAA. Extract of dry matter has higher toxicity effect than fresh extract. The application of wedusan extract is eviromental friendly and specific-range against pest. It is promising for future development as natural product in agriculture area.
The Correlation of Regulatory T (TReg) and Vitamin D3 in Pediatric Nephrotic Syndrome Nurtyas, Yunika; Subandiyah, Krisni; Fitri, Loeki Enggar
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.08

Abstract

Nephrotic syndrome (NS) is an autoimmune disease that correlates to the imbalance of regulatory T cells (TReg). This study was aimed to investigate the effect of vitamin D as adjuvant therapy of TReg population in pediatric nephrotic syndrome. This study was designed randomized clinical trial, double blind, with pre- and post-test control groups involving 15 subjects newly diagnosed with NS. Subjects were divided into 2 groups, namely K1 for group treated with prednisone+vitamin D and K2 group for prednisone treatment only. The population of TReg in peripheral blood mononuclear cells (PBMC) was analyzed using flowcytometry. Vitamin D serum level was measured through ELISA method. Results showed that there was a significant elevation of TReg (independent t-test, p = 0.010) in K1 group, which was higher than in K2 group. The Pearson test in the K1 group showed that vitamin D level was positively correlated with TReg (p = 0.039, r = 0.779).
Fruit Nutrients of Five Species of Wild Raspberries (Rubus spp.) from Indonesian Mountain’s Forests Surya, Muhammad Imam; Suhartati, Siti; Ismaini, Lily; Lusini, Yuyun; Destri, Destri; Anggraeni, Dian; Normasiwi, Suluh; Asni, Nurul; Sidiq, Mirwan Abu Bakar
Journal of Tropical Life Science Vol 8, No 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (709.404 KB) | DOI: 10.11594/jtls.08.01.13

Abstract

Rubus spp. (wild raspberries) is one of genera belongs to Rosaceae, also distributed in the mountain forest of Indonesia. It has a high potency to be cultivated plant. Although, some of the potential of Rubus has already known, information of about fruit nutrients of species that distribute in the mountain forest of Indonesia are not known yet. This study was aimed to determine the fruit nutrients of five species of wild raspberries that was collected in Cibodas Botanical Garden. The results of our experiments demonstrate that the fruits nutrients contents varied among five species of wild raspberries from Indonesian mountain's forests. Rubus fraxinifolius has the highest content of sugar (5.05 g sugar per 100 g fruits) compared to Rubus rosifolius, Rubus chrysophyllus, Rubus lineatus and raspberry. During the ripening and ripe period, there were a different value of vitamin C and iron contents. The highest vitamin C of R. fraxinifolius (83.65 mg/100 gram) on the ripening fruit stage II. On the other hand, the highest vitamin C of R. rosifolius (54.30 mg/100 gram) found on the stage of ripe fruit.

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