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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,001 Documents
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The Identity of the Herb 'Todda-Vaddi' in Van Rheede's Hortus Malabaricus: The Identity of the Herb ‘Todda–Vaddi’ Jisha Daniel; Ettickal Sukumaran Santhoshkumar; Madhavan Rajendraprasad; Sabarimuthan William Decruse
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.03

Abstract

The ambiguity on the botanical identity of Todda-vaddi in Rheede's Hortus Malabaricus is clarified as Biophytum reinwardtii (Zucc.) Klotzsch here. A detailed description, photographs and other relevant notes are provided. 
Mutation Analysis and Single Nucleotide Polymorphism of TP53 Gene in Breast Cancer in East Java Rizqa Radhiyah; I Kade Karisma Gita Ardana; Wisnubroto Wisnubroto; Dwi Listyorini; Hendra Susanto
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.12

Abstract

The Incidence of Indonesia breast cancer case in 2018 was reported at 20.7% or 160,653 in number. The factors that caused breast cancer is TP53 gene mutation and Single Nucleotide Polymorphism (SNP). This study aimed to determine the mutation and Single Nucleotide Polymorphism (SNP) of TP53 gene in breast cancer. Samples of this study were 9 people chosen based on a purposive technique. The methods include total DNA isolation, DNA quantification, PCR, and sequencing. The results of the sequencing were then analyzed using alignment and blast. The SNP is browsed by the SNP finder on NCBI both followed by protein modeling. The results of this study indicate the existence of mutation in the exon and intron regions. Substitution of Guanine (G) to base Adenine (A) is occur in codon 496 which is a coding region. SNP was also obtained in exon of sample 12. The conclusion of this study is breast cancer can be caused by mutations that occur in the exon and altered the structur of protein structure, so then affect the binding affinity between p53 and its target domain. Tp53 gene mutation is not the only cause of breast cancer staging development, there are other genes that also contribute to the development of cancer stage. In addition, Single Nucleotide Polymorphism in the TP53 gene can be used as a predisposition marker of breast cancer that has potential to be inherited
Biosorption of Cadmium by fungi isolated from Bharalu river, Assam Dipannita Deka; Pinky Bora; Nameirakpam Nirjanta Devi; Mayuri Chabukdhara
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.04

Abstract

Heavy metal pollution in the aquatic ecosystem poses a serious threat to the human health and aquatic biodiversity around. There is urgent need to remediate heavy metals contaminated wastewater through eco friendly ways. Fungi and other microorganisms have been reported to have promising potential to remove heavy metals from wastewater through biosorption. Thus the present work aimed to isolate tolerant fungal species from different sites of a polluted river Bharalu in Assam. After preliminary screening, out of a total of 15 fungal isolates obtained, 4 isolates Aspergillus niger, Aspergillus flavus, Aspergillus fumigates and Colletotrichum gloeosporioides were selected for further study. Tolerance limit of the isolates for Cadmium (Cd) was carried out at concentrations ranging from 100 ppm to 1000 ppm. Results showed that the maximum tolerance was observed in Colletotrichum gloeosporioides (approx. 8.8 mm), followed by Aspergillus flavus (approx. 5.8 mm) at 400ppm. The biosorption efficiency at 400ppm revealed that the maximum Cd concentration was absorbed by fungal biomass of Colletotrichum gloeosporioides (12.83 mg/g) and the minimum by Aspergillus niger (3.91 mg/g). Molecular identification was carried out followed by PCR amplification of the fungal biomass showing highest absorption. The sequence obtained was submitted in the GenBank using BANkIt with accession number-MN714368 which showed high similarities with Colletotrichum gloeosporioides. This results indicated that fungus are promising and potential sources for metal biosorption and detoxification.
Diversity of Foliar Endophytic Fungi in Artocarpus heterophyllus Lam. and Citrus reticulata Blanco of Tripura Sanchita Bhattacharya; Sanjit Debnath; Ajay Krishna Saha
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.15

Abstract

Present investigation dealt with the isolation and diversity of foliar fungal endophytes from two fruit yielding plants of Tripura state. Seasonal distributions of endophytes in respect to fruiting and non-fruiting season in two host plants were also studied. Twenty one fungal strains along with one nonsporulating hyaline form were isolated as foliar endophytes from the two host plants. In both fruiting and non-fruiting seasons, from Citrus reticulata seven foliar endophytic fungi were isolated. Whereas from Artocarpus heterophyllus in both the season eleven fungal endophytes with one nonsporulating hyaline form were isolated. The relative frequencies of isolated fungi from both A. heterophyllus and C. reticulata in fruiting and non-fruiting season significantly differed among the sampling sites, whereas relative frequencies individual endophytic fungus showed no significant differences among various sampling sites.
Increasing Growth Tiger Prawn (Penaeus monodon) Using Organic Diet of Sauropus androgynous Awaludin Awaludin; Ira Maya Abdiani; Ahmad Arsyidi; Rukisah Rukisah
Journal of Tropical Life Science Vol. 10 No. 3 (2020)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.10.03.09

Abstract

Sauropus androgynous contain chemical compounds that can increase the growth of tiger prawn. This study aims to determine the growth of tiger prawns after giv- ing S. androgynous leaves ethanol extract mixed in feed. The research method uses a completely randomized design (CRD) 4 treatments, three replications. Treatment with a dose of Treatment 1 (T1) Control without giving S. androgynous extract, Treatment 2 (T2) 5 gr/kg of feed, Treatment 3 (T3) 50 gr/kg of feed, Treatment 4 (T4) 100 gr/kg of feed. Proximate test results of S. androgynous extract crude pro- tein content of 10.83%, crude fiber 1.87%, crude fat 0.85%, ash content 1.94%, dry weight 93.62%, and extract material without nitrogen 78.13%. Based on the results of the study, the weight growth and growth length statistically showed significantly different (P < 0.05). The highest growth of tiger prawn at the end of the study was found in P4 by giving S. androgynous leaf extract with an average weight growth value (70.18 ± 9.57 g). While the lowest weight growth was observed in T1 with  an average weight growth value (32.62 ± 7.54 g). The highest length growth was found in T4 with average length (1.19 ± 0.10 cm), while the lowest length growth was seen in T1 with average length growth (0.62 ± 0.09 cm).
ISOLATION OF THERMOPHILIC LIPASE PRODUCING BACTERIUM FROM HOT SPRINGS AT THE EAST COAST OF PENINSULAR MALAYSIA Tengku Haziyamin Tengku Abdul Hamid; Nur Asimah Zainal Abidin; Nurmusfirah Hasan
Journal of Tropical Life Science Vol. 11 No. 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.01

Abstract

Hot spring is a natural habitat for thermophilic bacteria and is the major source of thermostable enzymes useful in industrial application. In Malaysia, the search for thermophilic organisms have been focused on hot springs especially on the west coast of the peninsular. In this work, lipase producing thermophilic microorganisms were isolated from east coast hot springs in the states of Pahang and Terengganu. All of the 7 isolates showed the ability to grow at 45°C and formed lipolytic zones on tributyrin agar plates. However, only isolate B2B2 and S1B4 were able thrive at higher temperatures of up to 65°C. Bacillus and Geobacillus species was found to be the dominant bacterium isolated from these hot springs. From La hot spring, isolate L1 (MT 645486), LH2, (MT645483), LH3 (MT645484), LH4 (MT 645485) and LH5 (MT 645487) were all closely related to Bacillus sp. (94 to 97%). Meanwhile, from Bentong and Sungai Lembing hot springs, isolates B2B2 (MT668631) and S1B4 (MT668632) were closely related to Geobacillus kaustophilus (98.5%) and Geobacillus thermoleovorans (98%), respectively. Strains from Geobacillus sp. were able to thrive at higher temperature and their thermostable lipases have properties useful for biotechnological applications.
Comparative Study of Morphology and Histology on Papuan Jellyfish Mastigias papua in Kakaban Lake and Sea Nurasmi Nurasmi; Ahmad Ridwan; Awaludin Awaludin
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.07

Abstract

Jellyfish are marine invertebrates belonging to the phylum Cnidaria. Papua Mastigias is a jellyfish from the Scyphozoa class. This study aims to observe differences in the differences in environmental parameters, morphology and shape of nematocyte cells using the histological method with (SEM) Scanning Electron Microscopy on Papua Mastigias jellyfish in the Kakaban Lake and Sea. Environmental parameters in Lake Kakaban and Sea Kakaban were measured for salinity, temperature, and pH. The results of the t-test analysis showed that there were significant differences in environmental parameters in Lake Kakaban and Sea Kakaban (p <0.05). Body diameter and length of jellyfish tentacles were measured and analyzed using SPSS 16 with a t-test. From the t-test analysis, results showed that for Mastigias Papua jellyfish in Lake Kakaban and jellyfish in the Kakaban Sea showed a significant difference in the diameter and length of the body tentacles (p <0.05). The SEM results of jellyfish in the Kakaban Sea showed that the tentacles of Mastigias Papua in the Kakaban Sea had nematocytes consisting of three forms, namely Microbasic isorhiza, Atrichouz isorhiza, and Merotrichous isorhiza. Mastigias Papua in Lake Kakaban has nematocyst cells, which comprise of one type, namely Microbasic mastigophoran, where the number of nematocytes is minimal and has a smaller size than the Papuan Mastigias jellyfish that live in the Kakaban Sea. Nemeatocyte cells are stinging cells in jellyfish. SEM results show that the jellyfish in Lake Kakaban cannot sting because the size of the jellyfish tentacles is reduced to smaller, fewer nematocyte cells and different shape nematocytes. Based on the results of this study, it can be concluded that there are differences in environmental parameters, body dimensions, tentacle length, and the size and number of nematocytes between Mastigias Papua jellyfish in Dunau and in the Kakaban Sea.
Diversity of Pteridophytes in Mount Timolan Protected Landscape, Zamboanga del Sur, Philippines Maricris G Cudal; Liberty Grace L Calimbo; Diosdado Sumibay Jr; Jaidel Salise; Jessa Gomez; Meraflor Pagaling; Jasper Locson; Joel Cahayagan; Jernol Seno; Jahuel Nathan Daculio
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.05

Abstract

This study serves as the first generation research on the rich yet not poorly known ferns and lycophyte species of the Mount Timolan Protected Landscape (MTPL). A total of 151 species from 62 genera and 24 families were recorded from December 2019-May 2019. Overall, the species of MTPL respectively holds 14%, 40% and 71% of the recorded total species, genera and families of ferns and lycophytes in the Philippines. The most represented families in terms of the number of genera is Polypodiaceae (10 spp.), whereas, the most represented genera in terms of the number of species were Selaginella (11 spp.). The results of the study showed that Mount Timolan Protected Landscape harbours number of some endemic species (17 spp.), critically endangered species (1 spp.), endangered species (3 spp.), vulnerable species (5 spp.), threatened, non-native species and economically important species (25 spp.). Based on conservation and ecological status of assessed ferns and lycophyte species, it is likely to strongly suggest to set species-specific conservation management strategy to protect the threatened species. However, additional studies and thorough field explorations should also be done to conduct a re-inventory on the pteridophyte species in order to arrive a more scientific results to establish a real profile of the ferns and lycophyte species of Mount Timolan Protected Landscape.
Maternal Diet During Pregnancy and COVID-19 Susceptibility of Offspring: The “Thrifty Phenotype Hypothesis” Connection Custer C Deocaris; Malona V Alinsug
Journal of Tropical Life Science Vol. 11 No. 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.07

Abstract

There is accumulating evidence suggesting that ACE2, the host cell receptor for the spike (S) protein of the SARS-CoV-2, mediates viral entry and infection, is under epigenetic control. Here, we discuss studies suggesting a nutritional strategy for down-regulating ACE2 expression in tissues of offspring through the phenomenon of maternal epigenomic reprogramming mediated by maternal diet. The "thrifty hypothesis" was first proposed by Hales and Barker, which posits that specific genes are programmed based on early-life experience to promote efficient fat deposition and storage in adulthood. Our analysis of the proposed mechanism for "early life programming" in this paper via nutritional modulation of histone acetylation and DNA methylation goes beyond the physiological consequence of boosting the innate cellular resistance to a viral transmission. During the pandemic, where there is still no specific antiviral drug or a widely disseminated vaccine for COVID-19, we hypothesize that an epigenomic nutrition approach may be a practical approach to help mitigate viral transmission offspring.
Multi-Drug Resistance Bacteria: A Case Study in Western Peninsular Malaysia Freshwaters Mohd Suardi Suhaimi; Kam Kar Yern; Mohd Farizal Ahmad Kamaroddin; Syahidatul Fazlina Samsul Rizal; Syamimi Nadiah Baharudin; Fatin Shahira Binti Othman; Nur Roslina Peer Muhammad Hussain; Nurul Liyana Hassan; Nurul Aqidah Salikan; Nur Hidayah Mohd Dzukhi
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.05

Abstract

The emergence of antibiotic resistant bacteria in aquatic environment has led to an increase in waterborne health risks to an alarming extent. This study attempts to investigate the population of certain antibiotic resistant strains in Peninsular Malaysia.  From the samples of 14 rivers from 7 different states in Peninsular Malaysia, a total of 203 isolates were successfully isolated. These are from rivers in Negeri Sembilan, Melaka, Selangor, Kuala Lumpur, Kedah, Kelantan and Terengganu with 35, 15, 37, 39, 24, 26 and 27 isolates, respectively. The isolates were tested for their susceptibility towards 6 broad spectrum of antibiotics which are gentamicin, ampicillin, rifampicin, chloramphenicol, tetracycline, and ciprofloxacin. A total of 117 isolates were identified to have more than 20% MAR index value with 47 of the isolates possess minimum MAR index value of 50%. Based on MAR index value, 59% of isolates are high risk threats indicating a serious emergence of antibiotic resistant bacteria in the rivers in Peninsular Malaysia. Isolates with MAR index value of more than 50% were selected for 16S rRNA gene sequencing for further identification.Based on 16s rRNA gene sequencing, the isolates are a mixture of pathogenic and commensals bacteria. This also implies that the environment especially rivers can be a reservoir for genetic jugglery

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