cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota malang,
Jawa timur
INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
 68   69   70   71   72 
Potency of Endophytic and Rhizospheric Bacteria of Akar Kucing (Acalypha indica Linn.) as Antibacteria against Klebsiella pneumoniae Fitriana Dewi Rahmawati; Suharjono Suharjono
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.06

Abstract

ABSTRACT Prevalence of pneumonia in Indonesia was 2% in 2018. Treatment of pneumonia using antibiotics caused resistant of pathogenic bacteria. Endophytic and rhizospheric bacteria of Acalypha indica Linn. can be used as alternative for treatment of pneumonia. This study aims to analyze the potency and identify of endophytic and rhizospheric bacteria of the Acalypha indica Linn as an antibacteria of Klebsiella pneumoniae. The research included isolation and purification of endophytic and rhizospheric bacteria of the Acalypha indica Linn, antagonistic assay of bacteria cell culture, antibacterial assay of bacteria metabolites; and identify of potential isolates based on 16S rDNA sequence similarity. The results showed that number of rhizosphere bacteria 6.83 x 105 CFU/gram was more than endophytic bacteria 1.78 x 104 CFU/gram. Diversity of rhizosphere bacterial 0.72 was higher than endophytic bacteria 0.62. The rhizospheric bacteria RU112B and RU315B had highest activity to inhibit growth of Klebsiella pneumoniae. Both isolates RU112B and RU315B were identified as Staphylococcus saprophyticus with similarity 99.83% and Luteimonas terrae with similarity 99.67% respectively. Keywords: Acalypha indica Linn, endophytes, Klebsiella pneumoniae, rhizosphere
ROLE OF ACADEMIC INSTITUTION TO INFORM REGIONAL-SCALE BIODIVERSITY CONSERVATION IN THE EASTERN PHILIPPINES Jonathan Jaime G Guerrero; Krizler C Tanalgo; Carlo Gabriel R Abante
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.14

Abstract

The Philippines is among the most threatened biodiversity hotspots. Developing effective conservation requires science-based evidence from field data and observations. Yet, many important biodiversity information remain unpublished, particularly from academic institutions and NGOs. Here, we synthesized 34-year data from biodiversity studies from Bicol University in Luzon Island, Philippines. We found a large number of studies that increased in the post-2000 period with the majority of the studies focused on community surveys and animal and wildlife studies. While there is a massive number of studies, we found clear disproportionate distribution in terms of geographic and thematic areas. Our results may be based on a regional level, but if taken carefully, it has important implications and applications to other higher education institutions in the Philippines in promoting biodiversity studies and conservation in the country.
Effect of alkaline protease produced from fish waste as substrate by Bacillus clausii on destaining of blood stained fabric Saravana kumari P; Reshma R
Journal of Tropical Life Science Vol. 11 No. 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.08

Abstract

Alkaline protease or peptidases are the largest group of enzymes in biological industry with variety of application in various industries to process of raw material, dehairing, diagnosis, extraction, food production and processing, destaining, etc., where the conditions prevails above neutral pH. Because of the wider applications the demand also increases for alkaline proteases, so required to produce in large scale cost effectively. An alkaline tolerant bacterial strain Bacillus clausii was isolated from fish waste and used for mass production of alkaline protease using fish waste homogenate as media. Preliminary study on optimization of conditions for the mass production carried out. The optimum temperature for protease production ranges between 25oC and 35oC and pH determined as 9. Along with fish waste homogenate, the mass production of extracellular alkaline protease from mobilized and immobilized cells of B. clausii carried out in production media, mixture of production media and fish waste homogenate and nutrient broth as standards. The recorded results showed that the maximum enzyme production obtained immobilized cells in nutrient broth media and followed by fish waste homogenate of 8900 U/ml and 8600 U/ml respectively. Purified enzyme yield was maximum obtained from production media 0.35 g/ml.  Blood stained cloth treated with immobilized enzyme completely removed stain compared to treatment with non-immobilized enzyme and commercially used detergent. So the current study suggests the usage of microbial alkaline protease in house hold detergent to replace usage of chemicals and save the environment from chemical pollutants.
Population flux of the house sparrow Passer domesticus Linnaeus 1758 in Chinnamanur town, Uthamapalayam taluk of Theni district, Tamil Nadu, India Mohamed Meeran; Syed Marjuk; Mohammed Byrose; Subramanian Arivoli; Samuel Tennyson; Syedali Fathima
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.09

Abstract

A thorough study of house sparrow behaviour and its population is essential to revive its population status which is the need of the hour. Therefore, the nature of sparrow population, their nests, water and food sources, interspecific competitions and noise pollution were taken into consideration in the present investigation, which documented its first research carried at Chinnamanur town, Uthamapalayam taluk of Theni district, Tamil Nadu, India. The study period was divided into summer and winter seasons and the survey was carried out for a period of nine months from April 2019 to December 2019 by point count and ebird data method. A total of 542 house sparrows were counted of which 250 was present in summer and 292 in winter. The overall house sparrow population in Chinnamanur town, related to the summer and winter seasons exhibited the range of correlation coefficient value to be 0.94 indicating a very strong positive correlation. Above all, the present study revealed that mobile phone towers were not the main reason for the decline of house sparrows, rather it was loss of habitat and food, urbanization, noise pollution, interspecific competition and conversion of agricultural lands into plots.
Phylogenetic Study of Bufonidae (Amphibia: Anura) from Sumatera and Asia Based on Cytochrome B Gene Djong Hon Tjong; Dewi Imelda Roesma; Silvia Indra
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.15

Abstract

Bufonidae is widely distributed amphibian family around the world, including Indonesia (Sumatra) and Asia. Sumatra is an island which has separated because of sea level increase and create gene flows barrier for amphibian in Sumatra and Asia. The purpose of this research is to analyse Bufonidae phylogenetic which exist in several locations in Sumatra and Asia based on gene cytochrome b analysis. Samples were collected from six localities in West Sumatra. DNA extraction and amplification has been done in Genetics and Cell Biology Laboratory, Andalas University, whereas DNA sequencing were analyzed at MacroGen USA DNA Sequencing Laboratory, South Korea. About 14 tissue samples of Bufonidae from West Sumatra has been analyzed. The relationship of Bufonidae phylogenetics in Sumatra and Asia were divided into three main clusters. Duttaphrynus melanostictus in Sumatra is closer to Duttaphrynus melanostictus from India with 0,3-0,5% sequence divergence and it is a group of paraphyletic with Duttaphrynus melanostictus from Vietnam, Taiwan, and China.
Predominant Bacterial Diversity in Rheumatoid Arthritis Rat After Treated with Caprine CSN1S2 Protein Eko Suyanto; Fatchiyah Fatchiyah
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.01

Abstract

Introduction: Rheumatoid arthritis (RA) is an autoimmune and systemic inflammatory disease influenced by microbial abnormalities (dysbiosis) in the intestinal that affect changes in metabolism and immune system disorders. This study aimed to investigate the predominant intestinal microbiota in complete Freund’s Adjuvant-induced rheumatoid arthritis rats after treated with caprine milk CSN1S2 protein through fecal analysis based on PCR-DGGE and to construct the phylogenetic tree of bacteria as the evolutionary relationship. Method: The experimental animals were divided into 6 groups with 2 types of rat model, namely control rat (C group, CM group, and CY group) and RA rat (RA group, RAM group, and RAY group). Predominant cultivable microbiota was obtained by direct culture and analyzed using PCR-DGGE with some specific primers. The DNA sequences were analyzed and aligned using bioinformatics software to construct the phylogenetic tree. Results: We found that Lactobacillus group significantly increased in the control rat model and the predominant intestinal bacteria in RA rats were Enterococcus group (Enterococcus faecium and Enterococcus faecalis). Conclusions: The caprine milk CSN1S2 protein influences the composition of microbiota in RA rats with the emergence of predominant bacteria that are considered species of the Bacillus group, closely related to Bacillus coagulans, which can be promoted the growth of B. coagulans to suppress pathogenic bacteria in the development of RA disease.
Dehalogenases for pollutant degradation in brief: A mini review sefatullah Zakary; Habeebat Adekilekun Oyewusi; Fahrul Huyop
Journal of Tropical Life Science Vol. 11 No. 1 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.01.03

Abstract

Dehalogenases are microbial enzyme catalysed the cleavage of carbon-halogen bond of halogenated organic compounds. It has potential use in the area of biotechnology such as bioremediation and chemical industry. Halogenated organic compounds can be found in a considerable amount in the environment due to utilization in agriculture and industry, such as pesticides and herbicides. The presence of halogenated compound in the environment have been implicated on the health and natural ecosystem. Microbial dehalogenation is a significant method to tackle this problem. This review intends to briefly describe the microbial dehalogenases in relation to the environment where they are isolated. The basic information about dehalogenases in relation to dehalogenation mechanisms, classification, sources and the transportation of these pollutants into bacterial cytoplasm will be described. We also summarised readily available synthetic halogenated organic compound in the environment.
Complex Compound with Transitional Metal of Akway Bark (Drimys piperita Hook f.) as Low Molecular Weight Scavenging Antioxidant: a Computational Study: Complex Compound of AkwayBark as Scavenging Antioxidant Hostalige Hutasoit; Dionysius Joseph Djoko; Sutiman B Sumitro; Simon Bambang Widjanarko
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.02

Abstract

Akway (Drimys piperita Hook f.) was known as an endogenous plant of Indonesia. This plant was investigated as free radical scavenger based on paramagnetic properties. This study aimed to identify the bioactive compound and the role of Fe metals ions to enhance the free radical scavenger capacity as Low molecular weight antioxidant (LWMA) of akway. This study was designed by ultra-performance liquid chromatography (UPLC) coupled with ultra-high-resolution time of flight-mass spectrometry detector (TOF-MS), docking (Pyrex and Discovery Studio 2016 Client) and pharmacokinetic properties prediction (SwissADME). UPLC-TOF-MS analysis showed that herbal akway bark has five molecular formula (C4H3N10O21-, C14H19N4O91-, C4H7N6O51-, C15H28NO31+, and C23H32NO71+). The docking was illustrated the complex binding akway formula with Fe and its role as a metal donor and metal acceptor. It also confirmed on pharmacokinetics properties prediction which molecular compounds of akway have aqueous solubility. It meant the herbal akway bark complexed to Fe could be a low molecular weight antioxidant and it could be escalated free radical scavenger capacity.
Radiation sterilization of Honey and Honey-Alginate Wound Dressing from Stingless Bee (Tetragonula biroi) Collected from Sta. Maria, Laguna, Philippines Davison Torres Baldos; Joseph Mallari Puno; Levelyn Mitos Mesa Tolentino; Djowel Recto Villanueva Montefalcon; Gilberto Tura Diano; Celia Oprenario Asaad
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.02.11

Abstract

This study was conducted to determine the effect of radiation sterilization on alginate wound dressing containing honey from the Philippine stingless bee, Tetragonula biroi. Our results show that a radiation dose of 30 kGy did not affect the antibacterial property of honey against Staphylococcus aureus. Electron-beam irradiation did not produce significant alterations in the physicochemical properties (pH, total soluble solids, and flavonoids); however, the total phenolics was significantly increased in honey with higher irradiation doses. Demonstrating that irradiation can be applied to honey with negligible physicochemical effects, honey was incorporated in alginate and exposed to a sterilization dose of 25 kGy using an electron beam facility.  Irradiation did not affect the physicochemical properties (pH, moisture content, gel fraction, moisture vapor transmission rate (MVTR), and fluid handling capacity) of the honey alginate wound dressing (HAWD). The perspectives for the potential use of irradiated HAWD as a natural product-based substitute for commercial wound care products may be considered.
Utilization of Various Organic Wastes as Liquid Biofertilizer Carrier Agents towards Viability of Bacteria and Green Bean Growth Novi Arfarita; Tsuyoshi Imai; Cahyo Prayogo
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.01

Abstract

The problem in the production of biofertilizers is that raw materials are cheap, easy to get and apply. Another problem is determining the viability of the consortium microbes in a biofertilizer formulation. This study aims to determine the bacterial viability of various liquid media originating from organic waste as a liquid biofertilizer carrier. Three indigenous bacterial strains under consortium for phosphate soluble (Pantoea ananatis strain 53 (BC32)), non-symbiostic Nitrogen fixation (Bacillus licheniformis strain S45) and stabilizing soil aggregate (Pseudomonas plecoglossidastrain PR19) were added to liquid biofertilizer. The study evaluated 10 treatments using a randomized design with three replicates. The treatments are as follows: Peptone,molasses, Compost wash from seaweed waste, Vermiwash, molasses + glycerol, compost wash from seaweed waste + glycerol, vermiwash + glycerol, molasses + PEG (PolyEthylene Glycols)1%, compost wash seaweed waste + PEG 1%, and Vermiwash + PEG 1%. This biofertilizer formulation (liquid) was kept for 16 weeks at optimumpH 5.5. VP3 (Vermiwash made from vermicompost + PEG 1%) treatment showed thebest viability of bacterial strains during the 16-week storage period. The pathogenicitytest using green bean seeds Vima-1 showed that all liquid formulations of biological fertilizers with the three consortium bacterial isolates did not show signs of diseases and demonstrated better growth than the control treatment. Compared to other treatments, the best growth of bacterial strains was detected with MP2 (Molasses + glycerol) treatment. Formulations using vermiwash and PEG appear to maintain bacterial viability in the formulation effectively. However, the formulation of molasses and glycerol exerts a stimulating effect on sprouts growth.

Page 70 of 99 | Total Record : 986

 68   69   70   71   72 

Filter by Year

2010 2025


Reset
Filter By Issues
All Issue Vol. 15 No. 2 (2025): In Press Vol. 15 No. 1 (2025) Vol. 14 No. 3 (2024): In Press Vol. 14 No. 3 (2024) Vol. 14 No. 2 (2024) Vol. 14 No. 1 (2024) Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol 11, No 3 (2021) Vol. 11 No. 3 (2021) Vol. 11 No. 2 (2021) Vol 11, No 2 (2021) Vol 11, No 1 (2021) Vol. 11 No. 1 (2021) Vol. 10 No. 3 (2020) Vol 10, No 3 (2020) Vol 10, No 2 (2020) Vol. 10 No. 2 (2020) Vol 10, No 1 (2020) Vol. 10 No. 1 (2020) Vol 9, No 3 (2019) Vol. 9 No. 3 (2019) Vol. 9 No. 2 (2019) Vol 9, No 2 (2019) Vol. 9 No. 1 (2019) Vol 9, No 1 (2019) Vol 8, No 3 (2018) Vol. 8 No. 3 (2018) Vol 8, No 2 (2018) Vol. 8 No. 2 (2018) Vol 8, No 1 (2018) Vol. 8 No. 1 (2018) Vol. 7 No. 3 (2017) Vol 7, No 3 (2017) Vol. 7 No. 2 (2017) Vol 7, No 2 (2017) Vol 7, No 1 (2017) Vol. 7 No. 1 (2017) Vol 6, No 3 (2016) Vol. 6 No. 3 (2016) Vol. 6 No. 2 (2016) Vol 6, No 2 (2016) Vol. 6 No. 1 (2016) Vol 6, No 1 (2016) Vol 5, No 3 (2015) Vol. 5 No. 3 (2015) Vol 5, No 2 (2015) Vol. 5 No. 2 (2015) Vol. 5 No. 1 (2015) Vol 5, No 1 (2015) Vol 4, No 3 (2014) Vol. 4 No. 3 (2014) Vol. 4 No. 2 (2014) Vol 4, No 2 (2014) Vol 4, No 1 (2014) Vol. 4 No. 1 (2014) Vol 3, No 3 (2013) Vol. 3 No. 3 (2013) Vol 3, No 2 (2013) Vol. 3 No. 2 (2013) Vol. 3 No. 1 (2013) Vol 3, No 1 (2013) Vol. 2 No. 3 (2012) Vol 2, No 3 (2012) Vol 2, No 2 (2012) Vol. 2 No. 2 (2012) Vol. 2 No. 1 (2012) Vol 2, No 1 (2012) Vol 1, No 2 (2011) Vol. 1 No. 2 (2011) Vol. 1 No. 1 (2010) Vol 1, No 1 (2010) More Issue