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INDONESIA
Jurnal Riau Biologia
Published by Universitas Riau
ISSN : -     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 51 Documents
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Analisis Sekuen Ekson 6-8 dari Gen Ferritin pada Tiga Varietas Padi (Oryza sativa l.) Asal Kecamatan Bantan, Bengkalis - Riau ROSLIM, DEWI INDRIYANI; DEANESIA, DITA; , HERMAN
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

One of the obstacles encountered in Bengkalis agricultural is the high content of Fe in the soil. Fe ionscan cause toxicity in plants if it is accumulated in an amount exceeding the tolerable limit of the plant.This study was aimed to compare the nucleotide sequences of the ferritin2 gene in some rice genotypesfrom Riau commonly growing in tidal land in Bengkalis with Fe-overload-tolerant rice variety (Mahsuri)and national rice variety which was Fe- overload -sensitive (IR64). The research was conducted at theGenetic Laboratory, Faculty of Mathematical and Natural Sciences, Riau University from January to May2014. The methods included DNA isolation, PCR, electrophoresis, and sequencing. DNA sequence dataanalysis was performed by using the BLAST and MEGA programs. This study got ± 750 bp DNAfragment of the ferritin2 gene which covering exons 6-8. Total number of SNPs obtained are as many as53 pieces and only found in introns and exons six. The farthest genetic distance was between Mahsuri andAmat Candu (0.171), followed by Sadani and Opium Amat (0.164). The shortest genetic distance wasbetween Korea and IR64 and also Sadani. Korea had the shortest distance to Nipponbare, otherwiseAmat Candu was the farthest distance from Nipponbare. Based on exons 6 to 8 sequence, Amat Canduwas classified as Fe- overload -sensitive rice variety, whereas Korea was a Fe- overload-tolerant ricevariety. Sadani was Fe-overload-moderate rice variety.Keywords : Riau, PCR, ferritin gene, Oryza sativa¸ tidal land
Taksiran Akumulasi Biomassa atas Permukaan pada Eksperimen Restorasi Lahan Gambut Bekas Terbakar, Area Transisi Cagar Biosfer Giam Siak Kecil-Bukit Batu, Riau, Sumatera Indonesia GUNAWAN, HARIS; MUDIYARSO, DANIEL; MIZUNO, KOSUKE; KOZAN, OSAMU; SOFIYANTI, NERY; INDRIYANI, DIAN; SEPTIANI, DIEN; LESTARI, ISKA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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The Giam Siak Kecil-Bukit Batu Biosphere Reserve was managed by zonation as a approach for multibenefitof management goals. This Bioshere Reserve has a fairly high carbon stock stored in its naturalpeat swamp forest ecosystems. Nevertheless, the condition of the peat swamp forest in the BiosphereGiam Siak Kecil-Bukit Batu currently has been destructed, especially in the buffer and transition zones.The threats of protected areas are not only coming from forest fire factors, but also from encroachmentand illegal logging. Therefore, it requires a recovery effort. This research aims to determine the survivalrate and to estimate the above ground biomass accumulation of the main tree species of peat swamp forestthat used to restore the burnt peat. The estimated total biomass accumulation on the surface was measured using allometric equations. Result of this research proved that Jelutung tree (Dyera polyphylla) has thebest survival rate among the trees (98%), while banana tree (Mezzettia parviflora) has lower survival rate( 35%). The highest total biomass accumulation was found in Jelutung (Dyera polyphylla) (499.015kg/ha/year, while the lowest total biomass accumulation was found in Meranti batu (Shorea uliginosa)(88.867 kg/ha/year). A total of nine pioneer species were identified i.e. Tenggek burung (Euodia sp),kayu ara (Ficus sp), mahang (Macaranga triloba), karet (Havea brasiliensis), sendayan (Scleriasumatrensis), alang-alang (Imperata cylindrica), senduduk (Melastoma malabathricum), paku-pakuan(Neprolepis hirsutula) and rumput teki (Cyperus rotundus). The total biomass accumulation of earlysuccession in all types of vegetation pioneer is 338,91 kg/ha.Key words: Above Ground Biomass Accumulation, restoration, vegetasion succession, burned peatland,Survival Rate.
Penaksiran Biomassa dan Karbon Tersimpan pada Ekosistem Hutan Mangrove di Kawasan Bandar Bakau Dumai MANDARI, DESTI ZARLI; GUNAWAN, HARIS; ISDA, MAYTA NOVALIZA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Mangrove forest ecosystem has a function as an absorber of Carbon dioxide (CO2) from the air and storecarbon in the form of biomass. The research was conducted in December 2014 until January 2015, andaimed to determine the total amount of above-ground biomass and the carbon stock as well as therelation of the three conditions of the development of mangrove forests in the area of Bandar Bakau,Dumai, Riau Province. The plot was made by using transect line plot method, 50 x 50 m in size with atotal of 3 plots along the coastline. Each plot contained 5 (five) sub-plots (10 x 10 m) that randomlydetermined. The results showed that along the transect line was found 10 species of mangrove, i.e.Xylocarpus granatum, Rhizophora apiculata, Rhizophora mucronata, Bruguiera gymnorrhiza, Avicenniaalba, Avicennia marina, Sonneratia alba, Lumnitzera racemosa, Lumnitzera littorea, and Scyphiphorahydrophyllacea. The vegetation was dominated by Xylocarpus granatum with a density of 2128 trees/haand Rhizophora apiculata with a density of 1472 trees/ha. The highest total biomass and carbon stockgenerated by X. granatum with total biomass 78.6 ton/ha equivalent to 39.3 ton of C/ha. The total value ofbiomass produced from the whole plot is 115.85 ton/ha with a total carbon content stored at 57.91 tonC/ha.Key words: Above-ground Biomass, Carbon stored, Mangrove density, Xylocarpus granatum
Tingkat Kerusakan Ekosistem Mangrove di Desa Teluk Belitung Kecamatan Merbau Kabupaten Kepulauan Meranti UMAYAH, SARI; GUNAWAN, HARIS; ISDA, MAYTA NOVALIZA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

The destruction of mangrove forest ecosystems in Teluk Belitung Village, Kepulauan Meranti, wascaused by various human activities such as industrial activities, illegal logging, and coastal erosion.Rehabilitation efforts was needed to preserve mangrove forests. The community involvement had animportant role in determining the success of mangrove rehabilitation. This study aimed to determine theextent of mangrove forest ecosystem destruction based on the density and mangrove forest cover.Furthermore, this research described local community participation level and their knowledge inrehabilitation efforts of mangrove forest ecosystem. This study was conducted in Teluk Belitung,Subdistrict of Merbau, Kepulauan Meranti from November 2014 to March 2015. This study usedobservation, questionnaire, and interview methods to investigate the destruction of mangrove forestsecosystem and local community involvement in mangrove forests rehabilitation. The results showed thatthe region has suffered damage to the mangrove with a total density value of 626.67 trees/ha andmangrove forest cover in all types of <50%. The level of public participation was affected by socialstatus, planning, attitudes, and community rules in Teluk Belitung.Keywords: mangrove, community, participation, rehabilitation
Induksi Tunas dari Eksplan Kotiledon dan Epikotil In Vitro Jeruk Siam (Citrus nobilis Lour.) Asal Kampar pada Media MS CAHYATI, SRI; ISDA, MAYTA NOVALIZA; LESTARI, WAHYU
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Citrus nobilis Lour. (Siam Orange) from Kampar is one of the main fruits in Riau Province that is highlyconsumed by many people, but nowadays its productivity is lower due to pests and diseases. In order tomaintain the availibility of citrus from Kampar and to conserve this plant, an effort that can be done is byusing in vitro culture with the use of a combination of growth regulator; cytokine and auxin usingcotyledons and epicotyl explant. This study aimed to determine the best explant source and to know theeffect of the concentration combination of BAP, kinetin, and NAA that optimal in inducing plant shoot ofSiam orange. This study was conducted at Laboratory of Integrated Biology, Department of Biology,Faculty of Mathematics and Natural Sciences using a completely randomized design (CRD) with variousconcentrations of treatments, and observed for 42 days. The results showed that the percentage of lifeexplants, swelling cotyledon, and epicotyl explants was 100%, the percentage of best shoot formation was60% on the epicotyls explant with 1.0 mg/l BAP + 0.5 mg/l kinetin + 0.5 mg/l NAA. The percentage ofcallus formation only found in epicotyls explants with the highest percentage (100%) at 2.0 mg/l BAP +0.5 mg/l kinetin +1.0 mg/l NAA.Keywords: Citrus nobilis Lour., cotyledons, epikotil, in vitro, shoot induction.
Keanekaragaman Kupu-Kupu di Kawasan Gunong Bonsu Kabupaten Rokan Hulu, Provinsi Riau BIBAS, ELPE; MUHAMMAD, AHMAD; SALBIAH, DESITA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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This study concerned the species diversity and abundance of butterflies in Gunong Bonsu, an area situatedin Rokan Hulu District, Riau Province. Sampling was conducted within the period of Januari-April 2015in four different habitat types, i.e. secondary forest, rubber jungle, palm oil plantation and tour sites. Twostandardized sampling methods were combined, i.e. sweeping and trapping using fruit bait along transect(100 m-long fixed transect in each selected site). A total of 1641 individuals were captured with 189butterflies species were identified, including 14 species of Papilionidae, 105 species of Nymphalidae, 19species of Pieridae, 14 species of Lycaenidae, and 19 species of Hesperiidae. Among these species, onlyTroides amphrysus (birdwing butterfly) which is protected by the law at national as well as internationallevel. The species diversity index for Gunong Bonsu area is very high (H’= 4,53).Keywords: abundance, butterflies, species diversity index
Seleksi Potensi Bakteri Probiotik Indigenus Riau sebagai Agen Biopreservatif Bahan Pangan WULANDARI, NUR ASYIAH; FIBRIARTI, BERNADETA LENI; ROZA, RODESIA MUSTIKA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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The use of synthetic preservatives is not safe for consumption and very dangerous for health.Lactic acid bacteria (LAB) is a probiotic bacterium that can produce bacteriocins asbiopreservatives agents. Bacteriocins have been widely applied as a food preservative, but itscommercial availability is still low and very expensive. This study was conducted to test the abilityof 23 isolates of local probiotic bacteria in Riau to produce bacteriocins. LAB total population wascalculated by Total Plate Count (TPC) method. LAB inhibition test against indicator bacteriaStaphylococcus aureus FNCC 0047 was conducted using well diffusion method. Bacteriocinsantimicrobial confirmatory test was performed at 100OC for 15 minutes, 30 minutes, and 60minutes. The result showed that the highest LAB population was PSG4 (22.45 x 106 cfu/ml) andthe lowest was SLK2 (4.75 x 106 cfu/ml). The greatest inhibitory activity against indicator bacteriaS. aureus FNCC 0047 was found in PSG2 (15.1 mm) and the smallest was in SLK2 (10.75 mm)with the activity of bacteriocins 2575 mm2/ml and 792.6 mm2/ml respectively. The bacteriocinsconfirmatory test showed clear zone of isolates JN5, PSG2, and SLK2 with the largest inhibitionzone was during heating for 30 minutes and 60 minutes (19.2 mm, 19.5 mm, and 16.8 mm) whilethe smallest was for 60 minutes and 15 minutes (12.8 mm, 15.6mm, and 14.7 mm).Keywords: Probiotic bacteria, biopreservatives, bacteriocins, Staphylococcus aureus FNCC 0047.
Jenis-Jenis Kupu-Kupu (Sub Ordo Rhopalocera) yang Terdapat di Kawasan Hapanasan, Kabupaten Rokan Hulu, Provinsi Riau CHAHYADI, ENNIE; BIBAS, ELPE
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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The objective of this study was to identify the diversity of butterfly (sub ordo Rhoplalocera) in Hapanasanpark, Riau Province. The study was conducted from January to February 2014. Imagos were collected inthe morning and afternoon. They were captured directly by using butterfly net. The result of this studyshowed that there were 48 Rhoplalocera butterfly species of 5 families, that consisted of Hesperidae (5species), Papilionidae (6 species), Pieridae (9 species), Nymphalidae (26 species), and Lycaenidae (2species). The most abundant species belongs to Nymphalidae family. In this study we found Troideshelena. This species is protected by. laws and recorded in Appendix II CITES.Key words : Butterflies, Rhopalocera, Hapanasan
Sebaran dan Kelimpahan Lipas Kayu (Panesthia angustipennis angustipennis) di Bawah Tegakan Akasia (Acacia crassicarpa) dan Hutan Alam pada Lahan Gambut NURARIFIN, IRFAN; MUHAMMAD, AHMAD; SALBIAH, DESITA
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

Residual wood under acacia (Acacia crassicarpa) plantation forest on peatland harbors diverse insects,among which is the wood-feeding cockroaches (Panesthia angustipennis angustipennis. Blaberidae,Blattodea). This insect appears to enhance nutrient cycling through consumption and maceration ofdecaying wood. The purpose of this study was to assess the distribution and abundance of this insect inthis pulpwood plantation and nearby natural forest remnants. Sampling has been done in plantationblocks representing <1, 2-3 and 5-6 year-old stands and also in natural forest. Results showed that woodfeedingcockroaches was most frequently found (80,0 %) in 2 year-old acacia stand, whereas it was leastfrequently encountered in natural forest. Likewise, it was most abundant (9,500 individuals/ha or 0.95individuals/m2) in the former, while it was least abundant (2,500 individuals/ha or 0.25 individuals/m2) inthe latter. In general, the insect was more abundant in the plantation forest than in natural forest.Key words: Pulpwood plantation forest, residual wood, wood-feeding cockroaches .
Seleksi Aktinomisetes Penghasil Protease dari Tanah Gambut Desa Langkai, Siak, Riau LINDA, TETTY MARTA; MARTINA, ATRIA; FEBRIANTI, BERNADETA LENI; , HERLINDA; , TABRI
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Actinomycetes is well known as secondary metabolites producer and hence it has high pharmacological andcommercial interest. In this study, a total of 14 isolates of actinomycetes from peat soils in Langkai Village,Siak, Riau had been selected for their ability to produce protease. Extracellular protease from Actinomycetesisolates was characterized by incubating the isolate in Nutrient Agar media containing casein 1%. Screeningof actinomycetes isolates showed that five isolates had the ability to produce a clear zone with the value ofproteolytic index (IP) ranged from 5.73 to 11.15. Isolates L313 showed the highest IP value (11.15), as wellas the highest protease production (0.041 U/ml) in Nutrient Broth media containing casein 1% after 6 daysincubation at room temperature.Key words: actinomycetes, clear zone, peat soil, protease, proteolitic index

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