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International Journal of Biosciences and Biotechnology

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Published by Universitas Udayana

ISSN : 23033371 EISSN : 26559994 DOI : -

Core Subject : Science, Social,

Biochemistry, Genetics & Molecular Biology Environmental Science

International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences

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Articles 7 Documents

Search results for , issue "Vol 5 No 1 (2017)" : 7 Documents clear

CVPDr DNA FRAGMENT AFFECT DIFFERENCES IN RESISTANT TO CITRUS VEIN PHLOEM DEGENERATION (CVPD) DISEASE, NUTRIENT DEFICIENCIES AND QUALITY OF FRUITS I Gusti Ayu Diah Yuniti; I Gede Putu Wirawan; I Nyoman Wijaya; Made Sritamin
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Citrus Vein Phloem Degeneration (CVPD) disease is a major obstacle in the effort to develop and increase the production of citrus fruits in Bali. The study on the polymorphism of CVPDr DNA fragment shows that the CVPDr DNA fragment is resistant factor againt CVPD disease. This study try to elaborate the difference in resistance led to differences in plant nutrients deficiencies in the citrus plant with CVPD disease. . Besides, there are also difference in the quality of fruit due to CVPD disease attacks such as water content, vitamin C content and antioxidants in citrus fruits, color, flavor, taste and texture and fruit into small, hard and sour taste.

EXPLORING STRIP INTERCROPPING POTENTIALS OF MAIZE-PULSE CROPS TO FIGHT CLIMATE VARIABILITY IMPACTS IN DRYLAND AREAS I Komang Damar Jaya; Sudirman Sudirman; Rosmilawati Rosmilawati
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Recent climate variability affects maize production in dryland areas. This study aimed to explore potentials of strip intercropping of maize-pulse crops in improving productivity of dryland areas. The study was conducted in dryland area of Gumantar village, North Lombok (8.253654 S, 116.285695 E). Soil in that area was categorized as poor soil with the following properties: 0.46% organic matter, 0.05% N total (Kejdhal), available P 11.25 ppm (Olsen) and exchangeable K 0.77 me%, pH 7.0 and field capacity 29% (%/V). Rainfall data were collected during the growing seasons of 2016/2016 and 2016/2017. A field experiment of maize-pulse crops strip intercropping was conducted during a dry season of 2016. The component crops in the strip intercropping were maize NK212, maize NK7328, mungbean Vima-1 and groundnut Hypoma-1. All component crops were grown as monocropping and strip intercropping of maize-pulse crops in 8.4 x 5.0m plot size for each treatment. To measure productivity of the strip intercropping, relative yield total (RYT) and benefit to cost ratio (B/C) were calculated. They were great variations in rainfall in the last two years. From the experiment, data showed that all the strip intercropping treatments have RYT and B/C values >1 meaning that strip intercropping of maize-pulse crops is more productive than monocropping and is feasible to be practiced in dryland areas. With the short growing period and their drought tolerant nature of the pulse crops, especially mungbean, the strip intercropping can be used to fight climate variability impacts in dryland areas.

POTENTIAL EFFECT OF MACRO ALGA Caulerpa sp. AND Gracilaria sp. EXTRACT LOWERING MALONDIALDEHYDE LEVEL OF WISTAR RATS FED HIGH CHOLESTEROL DIET Ketut Srie Marhaeni Julyasih; I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Seaweed has potential nutrient content such as carotenoids, vitamins, fatty acids, carbohydrates, minerals, and other essential substances. Carotenoids have important biological functions as an antioxidant, and immunostimulatory which can prevent the disease, anti-inflammatory, anti-stress, anti-aging, and protect the skin from the harmful effects of ultraviolet radiation. Seaweed generally consumed as a vegetable by people in Bali, known as the local name Bulung Boni (Caulerpa spp.) and Bulung Sangu (Gracilaria spp.).. So far there has been no report or results of research on the effects of extract ethanol of Bulung Boni (Caulerpa sp.) and Bulung Sangu (Gracilaria sp.) as an antioxidant that can prevent lipid peroxidation which can be seen in decreased level of MDA in liver tissue or blood plasma. Therefore it is necessary to determine of plasmaMDA level of Wistar rat after fed high cholesterol diet treated with extract ethanol of Caulerpa sp. and Gracillaria sp. This experimental study used completely randomized design. Research using total of 24 Wistar rats divided into six sample groups of equal size, all fed with a diet high in cholesterol especially in negative control. The study consisted of negative control group (standard diet), positive control group (high cholesterol diet), high-cholesterol diet with Caulerpa sp. extract dose of 20 mg and 60 mg/100 g, high cholesterol diet with Gracilaria sp. extract dose of 20 mg and 60 mg/100 g body weight rat per day.The study resulted that rats fed high cholesterol diet with treated extract ethanol Caulerpa sp. and Gracilaria sp. with a dose of 20 mg and 60 mg per 100 g body weight rat / day had plasma MDA level significantly lower (p <0.05) compared with rats fed high cholesterol diet without treated with extract of Caulerpa sp. and Gracilaria sp.

THE EXISTENCE OF ENTOMOPATHOGENIC FUNGI ON RICE PLANTS RHIZOSPHERE Noerfitryani Noerfitryani; Hamzah Hamzah
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Enthomophatogenic fungus is biological control. This research aimed to identify fungi on semi-organic and inorganic rice plants rhizosphere and proved as entomophatogenic fungi. This research was conducted in Biological control, Plant Pests and Diseases Department, Faculty of Agriculture, Universitas Hasanuddin, Makassar. Isolation of fungi with a modified insect bait method then tested in larvae, calculated mortality of larvae, Tenebrio molitor were infected by fungi were re-isolated and cultured in PDA media. The result showed that the average of T. molitor larvae mortality infected by enthomopathogenic fungi on semi-organic rhizosphere was around 24% and 8% on inorganic rhizosphere. The percentage mortality of larvae on semi-organic rhizosphere were infected by Fusarium sp. was around 20-60 %, Aspergillus sp. 60%-80% and Trichoderma sp. 80%, while in inorganic rhizosphere up to 100% were infected by Aspergillus spp.

MORPHOLOGY, PHYSIOLOGY AND MOLECULAR CHARACTERISTICS OF OIL PALM (Elaeis guineensis Jacq.) ENDOPHYTIC Bacillus sp. Fifi Puspita; Hadiwiyono Hadiwiyono; Susilo Hambeg Poromarto; Dewi Indriyani Roslim
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Endophytic bacteria are the bacteria that live in plant tissues. In oil palm tissue there are many types of endophytic bacteria and have a role that can be beneficial for the plant, one of them is endophytic Bacillus sp. The aim of these research was to obtain morphology, physiology and molecular characteristics of endophytic Bacillus sp. originating from oil palm tissue. Sampling was done by random simple sampling method. Isolation of bacteria was performed on plant tissues such as roots, midribs, stems and leaves of oil palm plants. The results of morphological characterization such as colony color, colony shape and colony edge show similarity in each isolate but there are differences in the surface morphology of the colony, where there are 6 isolates with convex surface and 6 isolates with flat shape. Physiological test results such as catalase test, oxidase test, starch hydrolysis test, motility test and temperature effect test on bacterial growth showed positive results in each isolate. Molecular characterization using 16S rRNA primers based on BLASTn shows that all isolates tested have similarities with Bacillus sp. Based on the phylogenetic tree it was found that the endophytic bacteria of Ba-B2 isolates were associated with Bacillus flexus with 100% consistency index grouped at a distance of 0.03 and Ba-P2 isolates were related to Bacillus substilis at a distance of 0.01 with an 89% consistency index.

EVALUATION OF LAND SUITABILITY FOR INCREASING PRODUCTIVITY IN DEGRADED UNDA WATERSHED, DISTRICT OF KARANGASEM, BALI Ni Made Trigunasih; I Nyoman Merit; Wiyanti Wiyanti; I Wayan Narka; I Nyoman Dibia
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

Evaluation of land suitability to increase the productivity of degraded lands in the Unda watershed aims: providing a database of land suitability for land use planning in support of sustainable agricultural development, providing spatial information, and recommendations in accordance with the land use plan of inhibiting factors that exist. Identification of characteristic land quality evaluation survey carried out by the method of land with exploration area of research followed by soil sampling at some sample of land units. Land suitability classification using the criteria of the Technical Guidelines for Evaluation of Land For Agricultural Commodities of Soil Research Institute (2003) is by matching between the haracteristics of the land with the requirements of growing plants was evaluated. Types of agricultural crops that are evaluated include plantation crops and food crops hotikultura fruits. Suitability actual land (land suitability based on survey data) are mostly classified as unsuitable (N), and marginally suitable (S3). Qualities/characteristics as the land that is the main limiting factor, among others: quality of land erosion, slope, water availability, temperature, rooting media (soil texture and coarse material). Sustainable land use planning can be recommended with agroforestry, accompanied by vegetative conservation measures because it is cheaper and easier to be carried out by farmers. Recommended cultivation effort is a combination of timber plants with horticultural crops of fruits with the provisions of at least 40% must be enclosed timber. Plant timber that can be developed between the other plants that are not too heavy canopy.

GENETIC DIVERSITY AND FRUIT QUALITY OF SEVERAL POMELO “JERUK BALI” (Citrus grandis L. Osbeck) CULTIVARS IN BALI Ida Bagus Komang Mahardika; I Nyoman Rai; Made Sudiana Mahendra; Rindang Dwiyani
International Journal of Biosciences and Biotechnology Vol 5 No 1 (2017)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

This study aimed to determine the genetic diversity and fruits quality of the "Jeruk Bali" cultivars grown in Bali. This research was conducted in all regencies and city in Bali, during 2016. Furthermore, several cultivars of “Jeruk Bali” were genetically analyzed based on RAPD markers using 10 primers. Analysis of the quality of fruit is based on physical properties and chemical content. Eighteen cultivars of "Jeruk Bali" obtained have a fruit morphological character with round, short round, and piriform fruit shapes, which are red, pink, cream and white flesh color. RAPD analysis results at 53% similarity level are grouped into 5 groups. The first group was only one cultivar, the second group consisted of 13 cultivars, the third and fourth groups were only one cultivar, while the fifth group consisted of two cultivars. The analysis of the diversity between cultivars based on the combination of physical and chemical properties of the fruit with hierarchy method on similarity level about 85% in a group is obtained by 4 (four) groups. Groupings by combination of physical and chemical properties of the fruit are not synchronized in their entirety with dendograms based on their genetic diversity. This illustrates the physico-chemical properties of “Jeruk Bali” fruit in general is not fully genetical expressed, but also influenced by conditions of environmental growth.

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