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INDONESIA
Jurnal Ilmu dan Kesehatan Hewan
Published by Universitas Udayana
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Health Professions Veterinary
Jurnal Ilmu dan Kesehatan Hewan menerima naskah ilmiah original (belum pernah dipublikasikan) dalam bidang veteriner atau yang terkait dengannya. Jurnal Ilmu dan Kesehatan Hewan dikelola oleh Program Studi Magister Kedokteran Hewan Universitas Udayana dan terbit dua kali setahun.
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 2 No 2 (2014)" : 7 Documents clear
Asosiasi Polimorfisme Mikrosatelit DRBP1 Gen BoLa (Bovine Leucocyte Antigen) dengan Ukuran Tubuh pada Sapi Bali Ni Wayan Patmawati; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Genomic selection has a potency for improving the genetic quality of livestock. In effort to find out a molecular marker or DNA segment which is linked with a certain phenotype, we examined the association of the polymorphism of microsatellite DRBP1 with the body size of Bali cattle. A total of 55 blood samples of sires was collected from the Breeding Center of Bali Cattle Improvement Office in District of Jembrana. Total DNA was extracted with QIAamp DNA blood mini kit. The locus was amplified using PCR technique and alleles were separated through PAGE 6%, and visualized with silver staining. We found 5 alleles which their length varied from 108 bp to 136 bp. Allele 122 had the highest frequencies (25.45%). The expected heterozygositiy (HE) and observed heterizygosities (HE), and the PIC were 0.783, 0.891 and 0.738 consecutively. Statistic analysis showed that the polymorphism of microsatellite DRBP1 had no significant association (p > 0.05) with body size of male Bali cattle. This indicates that the DRBP1 does not play a role in determining the body size.
Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed Macaque Populations in Bali Island, Indonesia I Nengah Wandia; I Gede Soma; I Gusti Agung Arta Putra
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

The long tailed macaques inhabit throughout Bali Island, however, recently they have been fragmented into many smaller local populations. In spite of that, there are just few reports that describe the mode of fragmentation and the genetic structure of the population. This research aimed to explore the polymorphism of microsatellite loci on Y chromosome in long tailed macaque populations in Bali Island using three human microsatellite primers, namely DYS390, DYS391, and DYS393. A total of 99 blood samples were collected from male long tailed macaques originated from 8 populations (23 from Pulaki, 11 from Bedugul, 13 from Mekori, 8 from Sangeh, 6 from Uluwatu, 11 from Alas Kedaton, 11 from Ubud, and 16 from Bukit Gumang). Total DNA was extracted using QIAamp® Blood Mini Kit. The polymorphism of the microsatellite loci was detected using PCR technique, and allelic variations were separated through 7% poly acrylamide gel electrophoresis. The result of the research showed that DYS390 and DYS393 loci were monomorphic (each had only one allele). The DYS391 locus had two alleles, therefore, this locus was polymorphic. It could be suggested that DYS391 locus could be used to study genetic variation in male long tailed macaque population in Bali Island.
Pengaruh Berbagai Konsentrasi Dimethylsulfoxide terhadap Kualitas Semen Beku Ayam Hutan Hijau Post Thawing Wayan Bebas; DND Indira Laksmi
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

The process of freezing and thawing on semen can lead to physical stress, often called cold shock, and couses the structural and biochemical damage that affecting cell function and ultimately lead to the death of the cell The aim of this study was to know the effect of the addition of various concentrations of dimethylsulfoxide (DMSO) as the intracellular cryoprotectant in phosphate yolk diluent on the post thowing quality of the green jungle fowl semen. The study used eight green jungle fowl semens which were collected with massage techniques. Semen was evaluated macroscopically and microscopically. Good quality semen was diluted with phosphate yolk which was added four different concentration of DMSO, namely 4%, 6%, 8%, and 10%. Semen was then filled and sealed in a mini straw (0.25 mL) with the concentration of 150.106 cells, and equilibrated at 4oC for 4 hours. The semen freezing was processed using conventional method. Evaluation was performed on post thawing semen. The evaluation of semen quality included the progressive motility and plasma membrane intact. Data were analyzed by analysis of variance. If there were any significant differences, the data were futher analyzed by Duncan test. The results showed that addition of DMSO concentration of 6% has resulted the progressive motility and intact plasma membrane higher significantly (P
Seroprevalensi Neospora caninum pada sapi bali yang dipotong di Rumah Potong Hewan (RPH) Denpasar Adryani Ris; Nyoman Sadra Dharmawan; I Made Damriyasa
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Recent years the infection of Neospora caninum parasite has been a significant reproductive disease on cattle. The purpose of this study was to determine the seroprevalence of Neospora caninum in Bali cattle that are slaughtered in Denpasar’s slaughter house.A total of 184 serums was collected and tested using enzyme linked immunosorbent assay (ELISA). The results showed that there were 2 serum samples (1.08%) contain Neospora caninum antibodies. While the remaining samples, 182 samples (98.92%), were negative. Though the neosporosis seroprevalence rate in Bali is very low, a sustainable surveylance must be carried out to mitigate the broadness of the disease distribution.
Polimosfisme Lokus Mikrosatelit BM1329 dan Hubungannya dengan Calving Interval pada Sapi Bali Made Raysa Merliana; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle plays an important role in supplying beef in Indonesia because it has good quality, high fertilization and low fat percentage. The aim of this research was to characterize the Bali cattle genetic using molecular marker of microsatellite BM1329 and its relation with calving interval. The data was taken from 19 cattles that belong to UD Sari Laba, Bangli, Bali. The association between BM1329 marker and calving interval was analyzed using General Linear Models concept. The result showed that the duration of calving interval for the Bali cattle was 314 – 451 days. There were 4 alleles found in the locus of microsatellite BM1329. The alleles size varied from 144 to 150 bp, in which, the allele 146 had the highest frequency (71.05%). The observed heterozigosity (Ho) and the expected heterozigosity (He) and the PIC were 0.474, 0.478, and 0.435 respectively. The statistical analysis showed that microsatellite BM1329 has no assocition with the calving interval (P>0.05).
Asosiasi Lokus Mikrosatelit BMS1282 dengan Kejadian Kawin Berulang pada Sapi Bali Yesy Febnica Dewi; I Nengah Wandia; I Ketut Puja
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Bali cattle has an important role to fulfill the meat demands in Indonesia. The improvement of molecular technology will facilitated bali cattle genomic selection. The aim of this research was to verify association between BMS1282 microsatellite locus and repeat breeding in bali cattle. Totalling 52 bali cattle blood samples was taken from UD Sari Laba, Bangli, Bali as DNA resources. DNA was extracted using blood mini Kit qiagen and microsatellite locus was amplified by Polymerase Chain Reaction (PCR). Alleles were separated with 6% PAGE and visualized using silver stain. There were 5 alleles in BMS1282 locus which the alleles size varied from 148 to 156 bp. Allele 152 was the higest frequency (54.81%) and allele 156 was the lowest frequency (1.92%). The value of heterozygosity (Ho), He, and PIC was 0.346, 0.626, and 0.569. consecutively. Statistical analysis revealed that BMS1282 locus has no assosiation with the repeat breeding (P>0.05). This result indicates that BMS 1282 locus has no important role in regulating the repeat breeding.
Potensi Serbuk Daun Kelor (Moringa oleifera) Sebagai Anthelmintik Terhadap Infeksi Ascaris suum dan Feed Supplement pada Babi Muhammad Ulqiya Syukron; I Made Damriyasa; Nyoman Adi Suratma
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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Abstract

Pig ascariasis is an intestinal parasitic disease caused by Ascaris suum. The economic losses in pig ascariasis are caused by a bad feed conversion ratio (FCR) and rejection of some organs after animal slaughtering. An anthelmintic utilization and farm management improvement are the common prevention action, however, recently the utilization of herbs as an athelmintic has been developed, one of them is Moringa oleifera leaves. Moringa oleifera leaves are also a potential for a sources of animal food because of their high nutrients. This intervention research aimed to examine the anthelmintic effect of Moringa oleifera leaves and its potency as feed supplement. Experimental design used was ccompletely randomized design split time (CRD Split Time) with six treatments namely Moringa oleifera 5% and an infection of infective larvae of A. suum (1), Moringa oleifera 5% (2), positive control (3), Moringa oleifera 10% (4), Moringa oleifera 10% and infection of infective larvae of A. suum (5), and no treatment as negative control (6). Each treatment was imposed on four female landrace piglets aged 8 weeks and weighed around 11 kg. The results showed that Moringa oleifera 5% and 10% of the feed could inhibit the egg production of A.suum and had a significant effect (P

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