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All Journal Media Veteriner Jurnal Ilmu dan Kesehatan Hewan Buletin Udayana Mengabdi Indonesia Medicus Veterinus Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences
I Gusti Agung Arta Putra
Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Karakteristik Lokus Mikrosatelit D8S1100 pada Populasi Monyet Ekor Panjang di Bukit Gumang, Karangasem, Bali. Rumba, Jefri Ndawa; Wandia, I Nengah; Arta Putra, I Gusti Agung
Indonesia Medicus Veterinus Vol 2 (1) 2013
Publisher : Faculty of Veterinary Medicine, Udayana University

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Abstract

Pengungkapan berbagai karakteristik genetik populasi memiliki nilai yang strategis. Selain merupakan khasanah informasi ilmiah, karakteristik tersebut dapat digunakan untuk memprediksi viabilitas masa depan suatu populasi, dan bahan pertimbangan untuk usaha-usaha konservasi. Mikrosatelit merupakan marka/penanda molekuler pilihan untuk studi pilihan genetik populasi. Mikrosatelit adalah segmen langsung dari materi genetik (DNA), sehingga penggunaannya sebagai marka molekuler akan lebih mencerminkan karakteristik genetik populasi. Penelitian ini dilakukan untuk mengkarakterisasi lokus mikrosatelit D8S1100 dan mendapatkan informasi awal mengenai status poplimorfisme sebelum dipilih sebagai marka molekuler dalam studi genetik populasi. Variabel yang diamati adalah jumlah dan jenis alel, frekuensi, dan heterozigositas. Sembilan belas sampel darah monyet dikoleksi dari di Bukit Gumang, Karangasem, Bali, sebagai sumber DNA. DNA total diekstraksi menggunakan QIAmp DNA Blood Mini Kit. Lokus mikrosatelit D8S1100 di amplifikasi dengan teknik polymerase chain reaction (PCR), sebanyak 30 siklus dengan suhu annealing 540C. Selanjutnya, alel dipisahkan dengan elektroforesis pada gel poliakrilamid 7% dan dimunculkan dengan pewarnaan perak. Hasil penelitian menunjukkan bahwa hanya ditemukan satu jenis alel pada lokus D8S1100 dalam populasi monyet ekor panjang di Bukit Gumang dengan panjang alel 196 pasang basa. Dapat disimpulkan bahwa lokus D8S1100 pada populasi monyet ekor panjang di Bukit Gumang bersifat monomorfik. Hasil penelitian mengindikasikan bahwa lokus mikrosatelit D8S1100 kurang baik digunakan sebagai marka molekuler untuk mengkaji variasi genetik populasi monyet ekor panjang di Bukit Gumang.
CM-10 Histopathological, Serological and Molecular Confirmation of APMV- 1 Infection I Gusti Agung Arta Putra; Anak Agung Ayu Mirah Adi
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Histopathological, Serological and Molecular Confirmation of APMV- 1 Infection
Polymorphism of Microsatellite Loci on Y Chromosome in Long-Tailed Macaque Populations in Bali Island, Indonesia I Nengah Wandia; I Gede Soma; I Gusti Agung Arta Putra
Veterinary Science and Medicine Journal Vol 2 No 2 (2014)
Publisher : Udayana University

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The long tailed macaques inhabit throughout Bali Island, however, recently they have been fragmented into many smaller local populations. In spite of that, there are just few reports that describe the mode of fragmentation and the genetic structure of the population. This research aimed to explore the polymorphism of microsatellite loci on Y chromosome in long tailed macaque populations in Bali Island using three human microsatellite primers, namely DYS390, DYS391, and DYS393. A total of 99 blood samples were collected from male long tailed macaques originated from 8 populations (23 from Pulaki, 11 from Bedugul, 13 from Mekori, 8 from Sangeh, 6 from Uluwatu, 11 from Alas Kedaton, 11 from Ubud, and 16 from Bukit Gumang). Total DNA was extracted using QIAamp® Blood Mini Kit. The polymorphism of the microsatellite loci was detected using PCR technique, and allelic variations were separated through 7% poly acrylamide gel electrophoresis. The result of the research showed that DYS390 and DYS393 loci were monomorphic (each had only one allele). The DYS391 locus had two alleles, therefore, this locus was polymorphic. It could be suggested that DYS391 locus could be used to study genetic variation in male long tailed macaque population in Bali Island.
Genetic Structure in Long Tailed Macaque Populations in The Region of East Java: Diversity of Mithochondrial DNA in Alas Purwo and Baluran Population I Nengah Wandia; I Gusti Agung Arta Putra; I Gede Soma
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

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Abstract

Long tailed macaque (Macaca fascicularis) has distributed throughout the regions in Indonesia, however,they, now, live separately to many local populations within an island. This research aimed to explore thegenetic diversity within and between populations of long tailed macaque in the Region of East Java. Thegenetic diversity was examined by molecular marker of d-loop region mtDNA using a pair of specificprimers. A total of 29 blood samples (15 samples from Alas Purwo population and 14 samples fromBaluran population) was extracted to find out a total DNA. The d-loop region mtDNA was amplifiedthrough PCR tecnique. The PCR products were sequenced using dideoxynucleotide chain terminationmethod at Macogen Inc. Korea. The research found 6 haplotypes, in which 3 haplotypes were found in eachpopulation. Haplotype and nucleotide diversities varied from 0.257 to 0.275 and 0.0012 to 0.0025repectively. Genetic differentiation between populatioon was F=44.1%. Phylogenetic reconstructionshowed that all haplotypes constructed  two different groups (haplogroups)  and there was no sharedhaplotype betwen populations. It can be concluded that both populations (Alas Purwo and Baluran) areuique and have low genetic diversities.
TEKNIK DAN MANAJEMEN PRODUKSI BIBIT BABI DI BANJAR DAUH UMA, DESA PENGOTAN, KABUPATEN BANGLI NLG SUMARDANI; IGA ARTA PUTRA; IW SUBERATA; IG SURANJAYA; DK HARYA PUTRA; WS YUPARDHI
Buletin Udayana Mengabdi Vol 14 No 1 (2015): April 2015
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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The short course of management of animal husbandry and using Artificial Insemination (AI) on swine wasconducted on September 6th 2014 at Dauh Uma Pengotan Village, Regency of Bangli. There were 15 farmers whomerged into group as participants of this activity. The aimed of this activity was to increase litter size of swinewith AI program in breeding management. The used method of activity was giving counseling about breedingand health management of animal husbandry in breeding farm, AI program, and then practiced inseminate onswine. Based on the process of short course it was concluded that, the response of participants on AI was veryenthusiastic in joining the activity.Keywords : animal husbandry, breeding, artificial insemination, litter size, swine.
VASEKTOMI DAN PEMOTONGAN TARING PADA MONYET EKOR PANJANG (Macaca fascicularis) DI KAWASAN WISATA PURA BATU PAGEH, DESA UNGASAN BADUNG I Nengah Wandia; I Gede Soma; I Ketut Suatha; I GA. Arta Putra; Sri Kayati Widyastuti; Aida Louise Tenden Rompis
Buletin Udayana Mengabdi Vol 11 No 2 (2012): Volume 11 No.2 – September 2012
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Population growth and aggressiveness are important factors on macaque populations that their habitats are used as tourist destinations. Controlling those two factors may decrease the negative impact of the macaque population both to the environment and the internal circumstance of the population. This Community Service was packed as a vasectomy and canine cutting services on the adult male of long tailed macaques occupying a tourist destination of Pura Batu Pageh, Ungasan Village, Badung Regency. Vasectomy is a surgical division of all or part of vas deferens to induce sterility. Surgical resection of part of vas deferens used a midline slicing method. In this program, one selectively adult male was successfully vasectomized and the four canines were also cut successfully. Later inspection showed that there was no any problem with the individual that formerly subjected to medical treatments and even the wounds went to be healing completely.
KONFIRMASI AVIAN PARAMYXOVIRUS TIPE 1 (APMV-1) SECARA HISTOPATOLOGIS, SEROLOGIS, DAN MOLEKULER (Confirmation of Avian Paramyxovirus Tipe 1 (APMV-1) Infection by Histopathology, Serology, and Molecular Method) I Gusti Agung Arta Putra; Anak Agung Ayu Mirah Adi
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.746 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5089

Abstract

Research was conducted to detect APMV-1 infection by examining microscopic lesions of chicken suspected ND and confirming the causative agent with serological and molecular assay. Samples obtained from commercial and back yard farm in 9 regencies and city of Bali Province were tested by rapid test for AIV antigen detection. AI negative samples were necropsied, then brain, lungs, and intestines were collected for histopathological examination. Samples tissue of brain, lung, spleen, and intestine were taken aseptically for viral isolation and amplification. Infected allantoic fluid was collected and tested by hemagglutination assay (HA) and hemagglutination inhibition (HI) test to prove APMV-1 serologically. Viral ribonucleic acid was isolated and subsequently reverse transcribed by reverse trasncription reaction followed by amplification by polymerase chain reaction to multiply the cDNA. Microscopically, perivascular cuffing (20%), endoteliosis (75%), and gliosis (75%) were found in the brain. In the lung, an interstitialis pneumonia (50%), lobar pneumonia (5%), and proliferation of pneumosit type 2 (100%) were observed. The most prominent intestinal lesions were catarrhal enteritis (75%) and hemorrhagic necrotizing enteritis (10%). Confirmation of the 20 isolates obtained in this study both serologically and molecularly were positive APMV-1. Moreover PCR results showed that 80% of its amplicon showed a single band and 20% still require some optimizations to get single good bands.
Selection of Bali Cattle as a Bull in Nusa Penida Island-Bali Based on Its Performance and Breeding Value Dewi Ayu Warmadewi; I Nyoman Ardika; I Gusti Agung Arta Putra
Journal of Veterinary and Animal Sciences Vol 2 No 2 (2019)
Publisher : Institute for Research and Community Service, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JVAS.2019.v02.i02.p03

Abstract

Selection program applied to improve the animal genetic potential. This program can be carried out based on the performance and breeding values ??of the economic trait of each individual. The research was carried out for 5 month (from May until September 2018). The purpose of this study was to find out the superior Bali bull by determining of chest girth male Bali cattle. This study used purposive random sampling method. The number of male Bali cattle with incisor of I0,I1,I2 used in this study were 147,29,19 heads respectively. Performance test was calculated by formula of BV = h2 ( Pi – P ) (Hardjosubroto, 1994). The results showed that the average of chest girth male Bali Cattle for I0, I1 and I2 were 135,65±16,98cm; 159,57±13,75cm and 166,26±19,94cm respectively. Based on the breeding value, male Bali cattle coded BK030 with I1 and BK101 with I2 were recommended as a superior Bali bulls because of their highest ranking.
Surgical Removal of Air Rifle Projectiles in Long-Tailed Macaques (Macaca fascicularis) in the Ubud Monkey Forest Sewoyo, Palagan Senopati; Wirata, I Wayan; Gorda, I Wayan; Wardhita, Anak Agung Gde Jaya; Bebas, I Wayan; Soma, I Gede; Putra, I Gusti Agung Arta; Wandia, I Nengah; Brotcorne, Fany
Journal of Applied Veterinary Science And Technology Vol. 6 No. 2 (2025): October 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I2.2025.158-162

Abstract

Background: Long-tailed macaques (Macaca fascicularis) frequently share their habitat with humans, leading to conflicts, especially when habitat disturbances force them to exploit human food sources. In response, humans sometimes use air rifles to deter these animals. Preventive measures such as sterilization programs, habitat restoration, and public education about responsible interactions with macaques are essential to reduce the occurrence of such incidents and promote coexistence. Purpose: This report describes the anesthesia and surgical management of gunshot wounds in long-tailed macaques. Case(s): Two adult free-ranging male long-tailed macaques in the Ubud Monkey Forest, Bali, Indonesia, were presented with gunshot wounds on the inguinal region and right caudal pelvic area, respectively. These macaques were captured and anesthetized for a scheduled vasectomy. A detailed clinical examination indicated that the projectiles were lodged in the subcutaneous tissue. Based on their shape and size, the projectiles were suspected to be 0.177 caliber (4.5 mm) air rifle pellets. Case Management: Surgical removal was performed to extract the air rifle pellets. Preoperatively, the macaques received amoxicillin (15 mg/kg BW, IM), ketorolac (2.5 mg/kg BW, IM), and Visine® eye lubricant. Propofol was used for anesthetic maintenance (1 mg/kg BW, IV). Following pellet removal, the wound was sutured with monofilament absorbable poliglecaprone 25, and the planned vasectomy was conducted using the bilateral incision method. Postoperatively, the macaques were administered atipamezole (0.02 mg/kg BW, IM) for anesthesia reversal, placed in recovery cages in lateral recumbency, and closely monitored. Conclusion: Once the macaques showed clear signs of recovery from anesthesia, they were transported back to the capture site and released into their original group.
Co-Authors Aida Lousie Tenden Rompis Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Bagus Bramardipa Anak Agung Gde Jaya Wardhita, Anak Agung Gde Jaya Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Bayu Setiabudi Brotcorne, Fany Dewi Ayu Warmadewi Dondin Sajuthi DWI SURYANTO DYAH PERWITASARI -FARAJALLAH I Dewa Ketut Harya Putra I G. Lanang Oka Cakra I Gede Soma I Gusti Ngurah Sudisma I Ketut Eli Supartika I Ketut Puja I Ketut Suatha I Made Kardena i Nengah Wandia I Nyoman Ardika I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Wayan Bebas I Wayan Gorda I WAYAN SUBERATA I Wayan Wirata Ida Ayu Putri Utami Ida Bagus Oka Winaya Jefri Ndawa Rumba Marissa Divia Dayanti Muh. Sofyan Muna Yuniarti Ni Luh Gde Sumardani Nyoman Sadra Dharmawan Palagan Senopati Sewoyo Sewoyo, Palagan Senopati Siswanto - Siswanto Sofyan, Muh. Sri Anggreni Lindawati Sri Kayati Widyastuti Suranjaya I .Gd TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Wayan Sayang Yupardhi