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Contact Name
Kadek Karang Agustina
Contact Email
k.agustina@unud.ac.id
Phone
+6281353306020
Journal Mail Official
bulvet@unud.ac.id
Editorial Address
Faculty of Veterinary Medicine Udayana University. PB Sudirman St campus, Denpasar, Bali Indonesia
Location
Kota denpasar,
Bali
INDONESIA
Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Articles 17 Documents
Search results for , issue "Vol. 11 No. 2 Agustus 2019" : 17 Documents clear
Efektifitas Fraksi Kromotografi Ekstrak Buah Pare (Momordica Charantia) Terhadap Peningkatan Bobot Badan Tikus Kondisi Diabetes Mellitus Cyrilus Jefferson Bour; Luh Made Sudimartini; I Nyoman Suartha
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.43 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p01

Abstract

The purpose of this research is to identify the effects the provision of faction chromatography extract of Momordica charantia on the increase in body weight white male diabetes mellitus rats. Momordica charantia extracted using ethanol 70%, then partitioned with n-hexane, and continued with thin layer chromatography, to achieve five factions. 40 white male rats (Rattus novergicus) of 3-4-month-old with 150-200 g of body weight were adapted for two weeks, arranged to eight the treatment groups which each group consists of 5 rats. Every treatment was checked blood glucose levels and the average of body weight on the day -1, 0 (hiperglikemia rats), 4, 11, 18. The condition of diabetes mellitus in every group except the negative control that was made by injecting streptozotocin (STZ) on day 0 at a dose of 45 mg/kg. The results of the study showed that the faction 1 and 5 pare fruit doses 50 mg/kg bb able to increased high significantly weight mice on the day 4th.. equal such as extract ethanol of pare fruit. Faction 2, 3 and 4 pieces pare with the same dosage are able to increase high significantly weight on the day 11th century. In conclusion, the results showed faction treatment 1 and 5 were faster respond of increasing weight compared with other fractions.
Penentuan Kadar Protein dan Fraksi Protein Crude Antigen Cysticercus cellulosae dari Isolat Lokal Leksana Aditya Kris Nugraha; Ida Bagus Ngurah Swacita; Ida Ayu Pasti Apsari
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.868 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p15

Abstract

Cysticercosis is a parasitic disease caused by tapeworm larvae, called Cysticercus. Cysticercus found in pigs is Cysticercus cellulosae which is the larvae of the tapeworm Taenia solium. The purpose of this study was to determine the levels and fractions of Crude protein antigen C. cellulosae. C. cellulosae used is local isolates obtained from infected pigs originating from Karangasem - Bali. Determination of protein content larvae were performed using an Invitrogen Quibit Fluorometer and determination of protein fractions larvae techniques using SDS-PAGE electrophoresis. The results showed that levels of the protein C. cellulosae from local isolate was 867 µg/ml. As for protein fractionation obtained 6 bands protein with a molecular weight: bands 1 = 120.5 kDa, bands 2 = 99.8 kDa, bands 3 = 60.8 kDa, bands 4 = 47.7 kDa, bands 5 = 35.6 kDa, and bands 6 = 23.3 kDa.
DETEKSI PENJUALAN DAGING AYAM TIREN DI EMPAT PASAR TRADISIONAL KOTA DENPASAR Luh Putu Pradnya Swari; Kadek Karang Agustina; Ida Bagus Ngurah Swacita; I Ketut Suada
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.282 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p06

Abstract

Tiren chickens are basically carcasses, namely chickens that die not because of slaughter, but chickens that have previously died due to poor endurance during the trip or affected by the disease then deliberately slaughtered for sale on the market. This study aims to detect the possibility of yesterday's sale of dead chicken meat (tiren) sold in four traditional markets in Denpasar City. The method used in this study is the purposive sampling method, which was a total of 72 samples of chicken meat were taken from four traditional markets in Denpasar City, each with three samples per market with six replications. The variables tested in this study were detection of tiren chicken with Malachite green test and pH test for chicken meat. The results showed that 19.44% (14/72) samples showed positive Malachite green test and average chicken meat pH 5.93 (5.32 - 6.45). Based on the results of the study it can be concluded that 19.44% chicken meat sold in four traditional markets in Denpasar City, it is suspected that tiren chicken is suggested to consumers to be more selective in choosing chicken meat for consumption
Variasi Genetik Lokus Mikrosatelit D7S1789 pada Populasi Monyet Ekor Panjang di Gunung Pusuk Lombok Barat Febio Tomasini Marciano Meus; I Nengah Wandia; I Ketut Suatha
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.148 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p11

Abstract

Long-tailed macaques (Macaca fascicularis) are Southeast Asian endemic animal with its behaviors and interactions form a protective cohesive group on its territory. Such patterns create distinctions that can be detected by microsatellite variations. This study revealed polymorphism of the D7S1789 microsatellite locus in a population of long-tailed macaques in Mount Pusuk West Lombok with the aim of knowing the genetic variation in the form of number of alleles, allele frequencies, heterozygosity and Hardy-Weinberg Equilibrium based on its allele’s distributing the Chi-square test. A total of 17 blood samples of long tailed macaque extracted used QIAamp DNA blood kit from QIAGEN to get DNA, DNA from locus D7S1789 microsatellite was replicated through 30 cycles of PCR technique, with annealing temperature 57°C. The alleles were raised by silver staining using 8% polyacrylamide gel. The results obtained two alleles with a frequency of 121 bp (0.794) and 130 bp (0.206), heterozygosity (0.337) and Hardy-Weinberg Equilibrium test (?2 count) was 0.422 (?2 table = 3.841; ? = 0.05; db = 1) which indicates a random mating population. It can be concluded that the D7S1789 microsatellite locus was polymorphic in the population of long-tailed monkeys in Mount Pusuk, West Lombok.
Anatomi Radiografi Normal pada Kaki Depan Landak Jawa Yuliani Suparmin; Gunanti Gunanti; Deni Noviana; Srihadi Agungpriyono
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.082 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p02

Abstract

The aim of this project is to develop a detailed accessible set of reference images of the normal forelimb radiographic anatomy in sunda porcupine (Hystrix javanica), including digit,carpus, metacarpal, radius, ulna, and humerus. This project that using 2 healthy sunda porcupines (male and female) was radiograped using digital radiography and standars projection. Four images, illustrating the normal radiographic anatomy of the forelimb were selected and presented along with detailed description. These image are aimed to be of assistence to veterinary surgeons, veterinary students, medic concervation especially in domestic wild animal, and veterinary researchers by enabling understand of the normal radiographic anatomy of the forelimb, and allowing comparison with the abnormal radiographic.
Deteksi dan Sekuensing Gen iroN, iutA, dan hlyF pada Avian Pathogenic Eschericia coli Nyoman Anandiya Ramaditya; I Nengah Kerta Besung; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.69 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p16

Abstract

Research has been carried out to detect pathogenic gene markers of Avian Pathogenic Eschericia coli (APEC) iroN, iutA, and hlyF in Escherichia coli bacteria isolated from organs of sick chickens in Bali, and to determine phylogenetic relationships between those marker genes in Bali and in the other countries in the world. Six isolates of E. coli bacteria with codes E2, E3, E7, E8, E9, and E10 were used in this study. The isolates were isolated from domestic chicken in 2018. All genes were detected using the Polymerase Chain Reaction (PCR) method. The genes of iroN, iutA, and hlyF could be detected from all isolates. Well readable sequence of iroN, hlyF, and iutA was 659 bp, 518 bp, and 250 bp, respectively. All three genes were homogenous. Phylogical analysis shows that all pathogenic markers share same cluster with the pathogenic E. coli from all countries in the world.
Studi Farmakokinetika Kodein Pada Kelinci Setelah Pemberian Intravena Bolus I Made Agus Gelgel Wirasuta; Ni Made Listiari; Dewa Ayu Swastini
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.693 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p07

Abstract

The codeine has been used as a marker for illicit heroin uses, that for increasing differentiating illicit heroin uses, it needs the codeine pharmacokinetic data. Eleven rabbits have been injected 11.04 mg codeine (with body weight: ± 1.8 kg) via marginal vena. Blood has taken at every 5, 15, 30, 60, 90, and 120 minutes. Serum has stored at -20 0C before analyzed. Blood protein was predicated by isoprophanol. Codeine was extracted with chloroform-isopropanol (3:1) and buffer phosphate 0.2N pH 9.3 then separated by phase toluene-acetone-ethanol-ammonia (45:45:7:3) as mobile phase and aluminium sheet of silica thin layer chromatography Gel 60 F254 as the stationary phase. Codeine determined by spectrophotodensitometry at 215 nm. Limit of detection and limit of quantification of codeine was 184.43 ng and 563.62 ng per spot, respectively the percentage recovery of in vitro was 60.60%. Profile concentration of codeine after IV bolus showed decreasing concentration at the first terminal and increasing codeine concentration at the last terminal that indicated an enterohepatic cycle. Codeine pharmacokinetic followed the first compartment with the half time was 30.61 minutes and the distribution volume was 7760.50 mL. Enterohepatic cycle caused a prolonged lifetime of codeine at the rabbit
PERFORMA FENOTIPIK KAMBRO HASIL PERSILANGAN ANTARA BETINA BROILER COBB 500 DAN JANTAN PELUNG BLIRIK HITAM I Wayan Swarautama Mahardhika; Budi Setiadi Daryono
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (664.387 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p12

Abstract

This research was conducted to measure the phenotypic performance of Kambro crossbreeds of Pelung Blirik Hitam and Broiler Cobb 500. Based on Body Weight (BT) measurement, Kambro population (n = 17) has an average BT of 1,244.14 ± 453.82 grams significant (p <0.01) to F1 Pelung (n = 7) with an average BT of 602.88 ± 79.93 grams in 8 weeks period with ad libitum diet of standard feed. Phenotypic performance of Kambro significant to F1 Pelung based on the measurement of linear bodyweight parameter, vitality parameter, PPa-PBe parameter and phenotype parameter. Kambro has the phenotype combination of parental generation based on phenotype parameter. PPa parameter was suitable BT estimation model based on non-linear quadratic regression (r = 0.956) with formula 1.84E3 ±3.54E2*x+31.73*x2. Difference between chicken group (p<0.014) was significant to BT and interaction between group and linear bodyweight parameter was not significant based on Analysis of Covariance. The mortality rate of Kambro was lower than F1 Pelung with the absent of vaccination in semi-intensive rearing system. As the size of hybrids population was limited, research findings must be validated with larger population size of hybrids.
The Gambaran Histologi dan Histomorfometri Limpa Kambing Peranakan Etawah Tri Ulfah Arema Yanti; Ni Ketut Suwiti; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (228.421 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p03

Abstract

The objective of this study to find out the histological and histomorphometry description of etawah crossbreed (PE) spleen. The spleens were collected from 10 male and female PE goats in Kampung Jawa slaughterhouse, Denpasar, Bali, then fixed in a solution of 10% Neutral Buffer Formalin processed for making preparation histology based on the method of Kiernan with hematoxylin-Eosin (HE) staining techniques. The goat's spleen consists of capsule, white and red pulp. Spleen histomorphometry of male and female PE goats obtained results, each having a capsule thickness of 128.58 ± 0.37 µm and 163.40 ± 0.35 ?m, trabecula thickness of 125.68 ± 0.13 µm and 131.45 ± 0, 28 µm, and white pulp diameter 466.63 ± 0.15 µm and 392.40 ± 0.30 µm respectively. It was concluded that gender affected the histology structure and histomorphometry of etawah crossbreed spleen.
Histopatologi Bronkiolus dan Pembuluh Darah Paru Mencit Jantan Pasca Terpapar Asap Rokok Elektrik Mia Monica; Anak Agung Ayu Mirah Adi; Ida Bagus Oka Winaya
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.988 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p08

Abstract

This study aims to determine the histopathological changes of bronchioles and blood vessel of mice (Mus musculus) lung post exposed of electric cigarette smoke (vaping). This study was used 24 mice that were divided into two treatment groups. Group I (P0) as a control without electronic cigarette smoke exposured and group II (P1) consistied of 12 mice as a treatment group, were exprosure with electric cigarette smoke. This study used a complete randomized design (RAL) split in time pattern with three 1st, 2nd and 3rd post-treatment sampling times. Four mice of each group were taken at each observation time. Lung tissue was taken to make preparations and stained with Hematoxylin-eosin (HE) staining. The results showed that there were significant differences (P <0.05) between the treatment group and the control group, but the duration of exposure did not have an effect. Mucosal thickness in the treatment group was higher than in the control group (P <0.05). Vessel lung endothelins and thrombosis. It can be concluded, exposure to electric cigarette smoke can cause degeneration, cell necrosis and increase the mucosal thickness of the bronchioles. The vascular system experiences endotheliosis, endothelial cell necrosis and thrombosis

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