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Kadek Karang Agustina
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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 587 Documents
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Histomorfometri Sel Darah Putih Agranulosit Bibit Sapi Bali Di Nusa Penida M. Oenas Adinugroho; Ni Ketut Suwiti; Anak Agung Sagung Kendran
Buletin Veteriner Udayana Vol. 11 No. 1 Pebruari 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.468 KB) | DOI: 10.24843/bulvet.2019.v11.i01.p06

Abstract

This study aims to determine about the histology structure and the morphometry white blood cells agranulocytes (lymphocytes and monocytes) of Bali cattle in Nusa Penida. A sample of blood from 50 female calves, had been taken through a jugular vein. The blood samples were fixed and colored by using Giemza staining method. The morphometry measurement was performed with was done under Axio Zeiss Imager 2 microscope with 1000x magnification. The results of the measurements were analyzed descriptively quantitatively, while the histology description was analyzed by qualitative descriptive. The results showed average mean lymphocyte diameter (9.22 ± 0.73µm) was smaller than monocytes (12.48 ± 1.73 µm). The existence of the lymphocyte and monocytes histologic structure difference which are found in the nucleus. The lymphocyte nucleus was round and filled the cytoplasm, whereas the monocyte nucleus formed a curve on one or two sides, thus, it did not fill the cytoplasm.
Uji Alergi Ekstrak Akar Tuba Terhadap Kulit Anak Kucing Lokal Siswanto Siswanto; I Nyoman Sulabda; I Gede Soma
Buletin Veteriner Udayana Vol. 8 No. 2 Agustus 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (64.726 KB)

Abstract

Tuba root (Derris elliptica) contains a toxin rotenone which is an isoflavone compounds (flavonoids) included in the group of phenolic compound, which can kill fish and insects, including ticks and mites. Tuba root can be used as antiektoparasit in animals (eg fleas in cats), however research on skin sensitization of kitten to tuba extract was less or even not exist. This research used experimental animals; 6 kittens aged 1-3 months (unnoticed sexes) were divided randomly into two groups, three as a control (K) and three as treatment (P). Cats shorn size of 0.5 cm x 0.5 cm in the nape, abdominal and groin, then drops (one drop) the tuba root extract concentration of 15%, 20% and 25%. After 30, 60, and 120 minutes, visible signs that the onset of allergic skin itchy, red, or inflamed. The design used was completely randomized analysis method uses a variant level of 5%. Further data tested by LSD 5%. Results showed that the tuba root extract concentration of 15%, 20% or 25% did not cause an allergic on kitten skin (not significant p>0.05). It concluded that the tuba root extract is safe and can be used for anti-parasite in kittens aged 1-3 months.
KARAKTERISASI MOLEKULER DAN UJI PATOGENESITAS STREPTOCOCCUS PATOGEN ISOLAT ASAL BALI I Gusti Ketut Suarjana; Widya Asmara
Buletin Veteriner Udayana Vol. 4 No.1 Pebruari 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.577 KB)

Abstract

The main of this study were characterized of muramidase released protein (MRP) andexctracellular factor (EF) as virulence factor of Streptococcus beta hemolityc of Bali islotesand its patogenecity on mice.The MRP was isolated from the cell walls bacteria withmuramidase (lysozyme) and EF was obtained from supernatant of bacteria precipated with70% ammonium sulphate and then dialysed. These protein were identified by usingsodium-dodecyl sulphate polyacrylamide gel electrophoresis ( SDS-PAGE). Isolates thatwas observed are five consist of three isolates from pigs and two isolates from monkeys.Pathogenecity test using 20 mices divided into four group. Group I inoculated with 0,1 mltodd-hewitt broth steril as negative control, group II inoculated with 0,1 ml inoculum ofbacteria of Streptococcus suis type 2 (strain D282) as positive control, group III inoculatedwith 0,1 ml Streptococcus beta haemolytic isolated from monkey and group IV inoculated with 0,1 ml Streptococcus beta haemolytic isolated from pig. The result of this studyshowed that all isolates were consist of eight protein bands of MRP and one EF of 110 kDamolecular weight. Eight protein MRP were 125 kDa, 76 kDa, 60 kDa, 57 kDa, 48 kDa, 45kDa, 30 kDa, and 28 kDa respectively. Each isolates had two major protein bands of MRP(76 kDa and 45 kDa).The patogenecity test in mice showed that the morbidity andmortality rates were 100% and 60% respectively. The prevalence of meningitis in mice are100%. Clinical sign were observed 30 hour post inoculated (pi) whereas mice found deathstarty 48 pi.
Bakteri Coliform dan Non Coliform yang Diisolasi dari Saluran Pernapasan Sapi Bali Putri Wiliantari; I Nengah Kerta Besung; Ketut Tono PG
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.222 KB) | DOI: 10.24843/bulvet.2018.v10.i01.p06

Abstract

The main purpose of this study is to determine the coliform and non coliform bacteria at the respiratory tract of Bali cattle that raised at the lowlands and highlands. 36 Samples were taken on calves, heifers, and adults, then grown on Salmonella Shigella Agar medium by spreading method. Coliform colonies and non coliform colonies were analyzed variably and followed by Least Significant Difference (LSD) test. The results showed that the number of coliform bacteria of bali cattle at the highlands was 1287.96 CFU/g and at the lowlands was 643.9798 CFU/g and non coliform bacteria at the highlands was 162.2464 CFU/g and at the lowlands as much as 81.12321 CFU/ g. Coliform and non coliform bacteria were obtained at the highlands and the lowlands showed no significant differences.
Hubungan Bagian-Bagian Panjang Kaki Depan Dan Belakang Pada Induk Sapi Bali Dengan Pedet Yang Dilahirkan I Made Edy Susanta; I Putu Sampurna; I Ketut Suatha
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.023 KB)

Abstract

This study aims were to determine the relationship between the parts of the front and rear foot length of bali calf with its parent. The object of the research consisted of 30 breeding calves and 30 individuals namely 15 male and female newborn respectively. Data in the form of measures footlong pieces of stem and parts of the calf leg length were analyzed using linear regression analysis. To determine whether there are differences between the parts of the front and rear foot length between the male with the female calf in a test with test T. The greater the ratio shows the development of long pieces of front and rear legs shows the earlier development. At birth the sizeof the back foot bottom, middle and top of the calf males and females, 63.7%, 46.5%, and 43.5% respectively of the size of its parent. Similarly, the size of the front foot down the middle and upperparts, 59.2%, 46.7%, and 44.9% respectively of the size of its parent. Development of parts of the front and rear leg starting from the bottom, middle and last at the top.
KUALITAS AIR MINUM TERNAK AYAM PETELUR DI DESA PILING KECAMATAN PENEBEL KABUPATEN TABANAN DI TINJAU DARI JUMLAH BAKTERI COLIFORM I Gusti Ketut Suarjana
Buletin Veteriner Udayana Vol. 1 No. 2 Agustus 2009
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.368 KB)

Abstract

A study was carried out to evaluate the water quality in layer farming at Piling Village,Distric and Regency of Tabanan, based on number of Coliform bacteria. The study wasblockrandomized design was asigned in this study. Water samples were collected fromthree sourcess, river, water reservoir and drinking water of pen.Samples were collectedin10 time.The Coliform analysed by most probable number (MPN) Method with nine tubeseries.The result of this study showed that number of Coliform in river, reservoir and place ofdrinking water were 11240 MPN/100 ml, 19800 MPN/100 ml and 35600 MPN/ 100 mlrespectively. Statisticcaly of this study showed amount of Coliform in drinking water offarm layer significant highest (P<0,01) than those reservoir and river. Amount of Coliformin reservoir significant highest (P<0,01) than those river.
Kualitas Air Peternakan Ayam Broiler Ditinjau dari Jumlah Bakteri Coliform dan Escherichia coli Eggy Hidta Lusandika; I Gusti Ketut Suarjana; I Ketut Suada
Buletin Veteriner Udayana Vol. 9 No. 1 Pebruari 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study aims were to determine the number of Coliform and Escherichia coli bacteria in drinking water of broiler chickens farm in the Mangesta Village, Penebel Sub-District, Tabanan. This study used a randomized block design from three sampling sites which were Beji (the water springs), water reservoir, and drinking place livestock, and had nine times repetition in each group. The number of Coliform and Escherichia coli bacteria was calculated by the method of spread. The data result from Coliform and Escherichia coli bacteria were analyzed using Analysis of Variance, followed by Duncan's Multiple Range Test. The research number of Coliform bacteria at Beji (water springs), water reservoir, and the drinking plate were 19768 x 105 CFU/100 ml, 11222 x 105 CFU/100 ml, 30957 x 105 CFU/100 ml and the number of Escherichia coli were 9233 x 105 CFU/100 ml, 3457 x 105 CFU/100 ml, and 19657 x 105 CFU/100 ml. Statistically, the number of Escherichia coli and Coliform in drinking place was significantly higher than the Beji (water springs) and water reservoir.
Profil Mineral Magnesium dan Tembaga Serum Darah Sapi Bali yang Dipelihara di Lahan Tegalan Ni Komang Dian Sri Sujani; I Wayan Piraksa; Ni Ketut Ketut Suwiti
Buletin Veteriner Udayana Vol. 6 No. 2 Agustus 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (82.633 KB)

Abstract

This study aims to determine the mineral magnesium and copper profile in blood serum of bali cattle farmed on dry land. Samples blood serum was taken from 15 bali cattles that farmed on dry land at Kecamatan Tejakula, Buleleng-Bali. Serums was analyzed by using wet destruction was H2SO4 and HNO3. Samples are read using by Atomic Absorpsion Spectrophotometry (AAS). The result shows that magnesium mean of 22,364 mg/l + 0, 005 mg/l, and copper mean of 0,087 mg/l + 0.001 mg/l. If this result compared to other cattle around the world, magnesium level is still in the normal range, while copper level more lower than normal level.
Penambahan Bovine Serum Albumin pada Pengencer Beltsville Thawing Solution terhadap Motilitas dan Daya Hidup Spermatozoa Babi Landrace I Made Agus Suryanatha; Wayan Bebas; Desak Nyoman Dewi Indira Laksmi
Buletin Veteriner Udayana Vol. 11 No. 2 Agustus 2019
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.055 KB) | DOI: 10.24843/bulvet.2019.v11.i02.p10

Abstract

This study aims to determine the effect of the addition of various bovine serum albumin (BSA) concentrations on betsville thawing solution (BTS)® extender to maintaining vitality and motility of spermatozoa on Landrace pigs. This study used Completely Randomized Design (CRD) with four treatments namely T0 (control): were given BTS® without BSA, T1: were given BTS® added BSA 5 mg/ml, T2: were given BTS® added BSA 10 mg/ml and T3: were given BTS® added BSA 20 mg/ml. Each treatment combination was repeated five times, with the total sample used was 20 samples. The measured variables in this study were the vitality and motility of Landrace pig semen. The results showed that the addition of BSA to BTS® diluents could increase the vitality and motility of spermatozoa on Landrace pigs. Where the optimal concentration was 10 mg/ml in keeping stability and maintaining the osmosis pressure stable
Titer Antibodi Anjing Lokal Enam Bulan Pasca Vaksinasi Rabies I Nengah Sudarmayasa; Ida Bagus Kade Suardana; I Nyoman Suartha
Buletin Veteriner Udayana Vol. 12 No. 1 Pebruari 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (128.987 KB) | DOI: 10.24843/bulvet.2020.v12.i01.p09

Abstract

Rabies is a zoonotic disease that can be transmitted from animals to humans. Most cases of rabies (98%) were caused by the bite of dogs infected with rabies, while the rest by the cats. This study aims to determine the antibody titer six months y.o local dog after the rabies vaccination using vaccines rabisin. This research was conducted in three stages, namely: taking blood samples in the villages of Buduk and Sading, serum sampling and serum testing using an enzyme linked immunosorbent assay (ELISA) test using 19 samples of local dogs with random blood sampling. The results showed that from 19 samples tested 17 samples (89%) had protective antibody titers (seropositive) and two samples (11%) did not have protective (seronegative) antibody titers. Seropositive was the value of Optical Density (OD) above 0, 5 IU, while seronegative is the value of Optical Density (OD) below 0.5 IU.

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