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Pro-Life
Published by Universitas Kristen Indonesia
ISSN : 23020903     EISSN : 25797557     DOI : 10.33541
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Ilmu Biologi merupakan salah satu ilmu yang terus bertumbuh dan menjadi bagian yang penting dalam kehidupan. Ilmu Biologi mencakup kajian teori, penelitian hingga pendidikan Biologi menjadi cakupan ilmiah yang terus diteliti dan dikaji hingga memperoleh manfaat bagi kehidupan. Program studi pendidikan Biologi FKIP-UKI ingin memberikan wadah bagi para peneliti baik di dunia Ilmu Biologi maupun Pendidikan Biologi untuk mempublikasikan hasil kajiannya. Jurnal ini terbentuk pada tahun 2014 dan menjadi referensi bagi para mahasiswa dan peneliti lain dalam melakukan penelitian. Bidang spesifik yang dicakup dalam Jurnal adalah Pendidikan dan Ilmu Biologi. Cakupan/ fokus masalah yang dicakup dalam Jurnal antara lain Pendidikan, Metode Ajar, Tumbuhan, Hewan dan Lingkungan. Jurnal terbit 3 kali dalam satu tahun, yaitu pada Bulan November, Maret dan Juli.
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Articles 222 Documents
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Ulasan Aspek Etnobotani dan Fitokimia pada Tumbuhan Andaliman (Zanthoxylum acanthopodium DC.) oleh Suku Batak Di Sumatera Utara Bernadheta Rumondang Saragih; Reza Raihandhany
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Andaliman (Zanthoxylum acanthopodium DC.) is an essential plant for the Batak people who utilize its fruit and seeds as spices for cooking, due to the tart taste. Apart of being food needs, andaliman is often used as a traditional medicine and traditional ceremonies. This research was conducted using a literature study method on various scientific using some keywords on the google scholar site. Aspects which were collected including the information on botany, ecology, distribution, local names, ethnobotany as local food and traditional medicine, and phytochemicals. Andaliman fruits and seeds are used as ingredients for cook such as arsik, sangsang, sup trites, gulai biawak, tasa telu, and kidu-kidu. In traditional medicine, andaliman is used to cure various diseases such as improving blood circulation, asthma, rheumatism, and increasing appetite. Besides for food and traditional medicine, andaliman can be utilized in agroforestry schemes as a plant to support natural environment conservation. Extract of andaliman fruit and seed contain various secondary metabolite chemical compounds, including alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids. In the end, information on the utilization of andaliman becomes a consideration for maintaining its existence.
Studi Keanekaragaman Invertebrata Di Kawasan Perairan Teluk Maumere Nusa Tenggara Timur Sitti Arafah; Hastuti Juniyati
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

The research objective was to determine the diversity of invertebrates in the waters of Maumere Bay, East Nusa Tenggara. This research was conducted in July-August using an exploratory descriptive approach. Sampling using purposive sampling technique. The results of the study found 150 species with descriptions: 26 species of Porifera phylum, 22 Coelenterata phylum, 67 Mollusca phylum, 5 Arthropoda phylum, and 30 Echinodermata phylum. Ecological parameters: Invertebrate density 2.05/m2-0.227/m2, relative density (RD) 0.017-0.001. The highest density value was from the type of Conus teselatus and the lowest density was from the type Aphrocallistes vastus. Frequency 0.017-0.001, relative frequency (RF) 0.015-0.001 with the highest frequency value of the Conus tesulatus type and the lowest frequency of the Aphrocallistes vastus type. The highest Importance Value Index for Conus tesulatus is 0.033 and the lowest for Aphrocallistes vastus is 0.002. The Shannon-Wiener species diversity index (H') value is 784,562 or the high richness category and the evenness value (E) is 77,856 which means that quantitatively the density value among community members is evenly distributed (E passes 1). The distribution pattern of all types of invertebrates is grouped with a Morisita Index (IM) value of 11.46-38.78 or an IM value > 1. The diversity of invertebrates in the study area is strongly influenced by environmental factors such as temperature, pH, salinity, and substrate which can reflect good conditions for the growth of invertebrates.
Pemanfaatan, Toksisitas dan Glikosida Jantung dari Digitalis purpurea L Marina Silalahi
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Digitalis purpurea (Schrophulariaceae) is used by the local Indonesian community as an ornamental plant, even though this plant has high toxicity and has the potential to treat heart problems. This study aims to explain the botany, bioactivity and toxicity of D. purpurea based on literature studies. Literature was obtained online, especially Google scholar using the keywords Digitalis purpurea, bioactivity D. purpurea, and glycoside heart. In Europe, D. purpurea is very popular to treat congestive heart failure and has been used since 200 years ago, but it is very toxic to humans. Digitalis poisoning causes anorexia, nausea, vomiting, and diarrhea, yellow or jaundice vision, appearance of blurred lines, decreased heart rate, and weight loss. D. purpurea's bioactivity includes overcoming heart failure, antioxidants, anti-cancer/tumor. Cardiac glycosides present in D. purpurea include cardenolides, digitoxin, gitoxin, purpureaside A, purpureaside B, acteoside and purpureaside C, which are related to their bioactivity. The toxicity and inotropic effects of cardiac glycosides result directly from inhibition of membrane-bound Na+K+_ATPase, an enzyme involved in the transport of Na+K+ across cell membranes. The use of D. purpurea needs strict control so that side effects can be minimized.
Isolasi dan Uji Patogen Bakteri Asam Laktat (BAL) dari Usus Ayam Kampung yang Dipelihara Secara Intensif Di Kefamenanu Welsiliana; Charles Venirius Lisnahan; Lukas Pardosi
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aimed to isolate LAB from the intestines of native chickens and to test the ability of LAB activity against pathogenic bacteria. Bacterial isolation was carried out by taking chicken intestines, then diluting up to 102. The results of the dilution were taken 1 ml to be inoculated into MRSA media using the pour plate technique, streak plate technique was used to obtain pure cultures and then incubated at 37˚C for 24 hours. LAB culture testing for pathogenic bacteria was carried out using MHA media containing Escherichia coli cultures, then incubation for 120 hours at 37˚C. Observation parameters included colony shape, colony color, number of LAB colonies, the diameter of the clear zone and LAB colonies. The results showed that the bacteria isolated from the intestines of native chickens were LAB which had around colony shape and were yellowish-white (beige)-milky white. The number of LAB colonies obtained was 3.7 x 105 CFU/ml with the best inhibitory ability of pathogenic bacteria found in USA 3 isolates (1.85 cm clear zone; 0.90 cm colony size) after incubation for 120 hours.
Eksplorasi Fungi Endofit dari Tanaman Sambung Nyawa (Gynura procumbens (Lour.)Merr.) Ranti; Ummi Hiras Habisukan; Amin Nurokhman
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Sambung nyawa (Gynura procumbens(Lour.) Merr.) is a plant that can be used as herbal medicine such as healing cuts, lowering blood sugar levels, facilitating menstruation, and preventing cancer formation. The various properties possessed by sambung nyawa plants make these plants continue to be used and experience plant scarcity. Another alternative is to use endophytic fungi which are known as a source of efficacious medicinal secondary metabolites but have not been widely used. This study aims to isolate and identify the genus of endophytic fungi on sambung nyawa plants. The method used in this study was sampling, namely root organs, root bark, stems and leaves of sambung nyawa plants, making media using instant PDA media mixed with aquadest and antibiotics, endophytic fungi using direct planting techniques on PDA media in cups. petri, purification of endophytic fungi by eliminating fungi that are considered to be the same from their morphological characteristics until truly pure fungi are found, endophytic fungi help macroscopically by observing the color, texture, and pattern of the colony then observing microscopically using the culture slide technique then observing under a hyrox microscope by observing the type of spore, spore shape and hyphae. The results of this study were 12 isolates of endophytic fungi identified in 10 different genera, namely Nigrospora, Blastomyces, Fusarium, Pythium, Cladosporium, Trichoderma, Gliocladium, Papulaspora, Aspergillus, Monacrosporium.
Peranan Mikroorganisme Pendegradasi Plastik: Tinjauan Biodegradasi Plastik, Mekanismenya, serta Mikroorganisme yang Berperan Putu Cindy Arista
Jurnal Pro-Life Vol. 10 No. 1 (2023): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Plastic is difficult to decompose in nature because it is a synthetic polymer made by humans and is made from petroleum. The problem that arises from the uncontrolled use of plastic is the accumulation of plastic waste in the environment which is not handled properly and becomes a source of environmental pollution. Plastic waste creates various kinds of environmental problems that disturb the balance of ecosystems and have an impact on the life of organisms in the environment. To overcome this problem, it is necessary to carry out related research as an effective solution for dealing with plastic waste. Biodegradation is one of the right methods for dealing with plastic waste now and in the future. However, there is limited review of the bio-decomposition of plastics, the microbial species present, the effectiveness of degradation, and the mechanisms involved. Based on this, this research focuses on a brief review of plastic biodegradation such as the factors that affect biodegradation, the species of microorganisms that play a role in the degradation of macro and micro-plastics, as well as the mechanism of plastic degradation through enzymes from the viewpoint of microorganisms. Various species of microorganisms such as bacteria, algae and fungi have the ability to decompose plastics but they cannot mineralize plastics completely. Meanwhile, research on the enzymes involved in plastic degradation is still limited, especially in the case of algae. This review can be a reference for researchers to find effective strategies to address the problem of plastic waste worldwide.
Keanekaragaman Hama Dominan pada Tanaman Padi (Oryza sativa L.) Di Langsa Timur, Aceh Nurjannah; Isnijar, Wahyu Firmana; Salwa, Zakia Hanifah; Sari, Herlina Putri Endah; Purnamawati, Jatu
Jurnal Pro-Life Vol. 10 No. 2 (2023): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Oryza sativa L. is a rice-producing food commodity plant that has high economic value, because of its function as staple food, source of income and source of nutrition for most of Indonesia's population. The availability of rice must always be guaranteed, but often encounters obstacles, one of which is the presence of pests. The purpose of this study was to determine the diversity and dominant pests species on rice plants in 3 villages, i.e Sukarejo, Alue Pineung and Cinta Raja Village, East Langsa District, Aceh. The method used in this research is descriptive explorative field observation using hand collecting sampling. Data analysis was carried out by describing pests, symptoms of attack and continued by calculating the value of the Shannon-Wiener diversity index. Based on the observations, the result showed 6 species of pests were found on rice which consist of 5 orders and 6 families, they are Leptocorisa acuta, Nezara viridula, Oxya serville, Lonchura leucogastroides and Pomacea caniculata. The diversity index values ​​in 3 villages were 1.41-1.72 with highest diversity index value from Alue Pineung Village. Of the 6 types of species, the dominant pest causing the most damage was Leptocorisa acuta (Walang sangit). This research is expected to be a reference in preventing pest attacks in the Langsa. Keywords: Diversity of pests, dominant pests, rice
Efektivitas Pemberian Zat Pengatur Tumbuh Campuran Bawang Merah (Allium cepa L.) dan Bonggol Pisang (Musa paradisiaca L.) terhadap Pertumbuhan Stek Batang Petunia sp. Fitriani, Linna; Riastuti, Reny Dwi; Arisandy, Destien Atmi; Jayati, Ria Dwi
Jurnal Pro-Life Vol. 10 No. 2 (2023): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

This study aims to determine the effectiveness of giving Growth Stimulating Substances on the Growth of Cuttings of Petunia sp. The population in this study is Petunia Plants. The samples were petunia plant cuttings soaked in a natural ZPT solution mixed with shallots and banana weevils. Data collection techniques used observation sheets. The results of the study were analyzed using one way Anava.. Based on the results of the study it was found that the percentage of growing cuttings in the ZPT treatment of mixed shallots and banana weevils and harmonic ZPT had the same level of growth percentage of cuttings in the medium category. Based on statistical analysis on shoot length, the value of F = 4.393 was obtained with a significance of 0.047. In calculating the root length, the value of F = 33.831 is obtained with a significance of 0.000. Based on the results of the research that has been done, it can be concluded that the administration of a mixture of growth regulators (ZPT) of shallots (Allium cepa L) and banana weevil (Musa paradisiaca, L) is effective on the growth of petunia stem cuttings). Keywords: Effectiveness, growth, natural ZPT, Petunia sp.
Kualitas Mikrobiologi Daging Se’i Sapi melalui Metode Curing menggunakan Ekstrak Daun Jati (Tectona Grandis L.f.) pada Penyimpanan Suhu Ruang Kia, Kristoforus. W.; Fobia, Kaprisius G.; Pardosi, Lukas
Jurnal Pro-Life Vol. 10 No. 2 (2023): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Tanaman jati (Tectona Grandis L.f.) merupakan salah satu tanaman alami jenis pepohonan yang hanya dimanfaatkan batangnya dalam bidang industri, sedangkan daunnya yang memiliki segudang manfaat jarang dimanfaatkan oleh masyarakat sehingga menjadi limbah yang terbuang. Daun jati secara tradisional sering digunakan untuk membungkus bahan makanan seperti daging, ikan dan tempe selama proses fermentasi untuk mencegah terjadinya pembusukan karena mengandung senyawa antibakteri. Penelitian bertujuan mengetahui aktivitas antimikrobial ekstrak daun jati terhadap pertumbuhan bakteri E. coli dan kualitas mikrobiologis daging se’i sapi. Variabel yang diteliti yaitu uji aktivitas antimikrobial ekstrak daun jati terhadap bakteri E. coli, perhitungan jumlah koloni (TPC) dan pengamatan keberadaan bakteri E. coli. Data diolah dengan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Metode penelitian menggunakan Rancangan Acak Lengkap. Perlakuan yang diuji yaitu: P0 = pembuatan dengan penambahan aquades (Kontrol -), P1 = pembuatan se’i dengan penambahan nitrat 0,6 g (kontrol +) dan P2 = pembuatan se’i ditambahkan ekstrak daun jati 0,5%. Hasil menunjukan bahwa konsentrasi ekstrak daun jati 0,5%, 1% dan 1.5% memiliki kemampuan bakteriostatik dengan kategori kuat dan rerata zona bening yang diperoleh yaitu 12.6 mm, 15.4 mm, dan 19.5 mm. Kontrol tidak memiliki kemampuan menghambat pertumbuhan bakteri. Hasil uji mikrobiologi dengan perhitungan TPC menunjukkan bahwa ekstrak daun jati memiliki kemampuan bakteriostatik dengan rerata yang diperoleh 92.0 CFU/g pada konsentrasi 0.5%. Sedangkan pada kontrol (-) dan (+) menunjukan nilai tertinggi yaitu 201.3 CFU/g dan 169.7 CFU/g. Hasil uji cemaran bakteri E. coli menunjukkan bahwa ekstrak daun jati memiliki kemampuan bakteriostatik dengan nilai rerata yang diperoleh 13.3 CFU/g pada konsentrasi 0.5%. Sedangkan pada kontrol (-) dan (+) menunjukan nilai tertinggi yaitu 55.4 CFU/g dan 38.0 CFU/g. Disimpulkan bahwa ekstrak daun jati memiliki kemampuan antimikrobial terhadap bakteri E. coli dengan terbentuknya zona bening pada konsentrasi 0,5%, 1% dan 1.5%, memiliki kemampuan dalam menghambat pertumbuhan koloni bakteri pada se’i sapi. Kata kunci: Antimikrobial, E. coli, ekstrak daun jati, se’i, zona hambat
Efek Pemberian Pupuk Organik Cair Berbasis Kulit Buah (Eco Enzyme) terhadap Pertumbuhan Tanaman Pakcoy (Brassica chinensis L.) Sinaga, Wulan Sari; Limeranto, Desyana Millenia; Pangala, Eugenia Larissa Bakti; Madyaningrana , Kukuh
Jurnal Pro-Life Vol. 10 No. 2 (2023): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Abstract

Household organic waste can be used as material for organic fertilizer for supporting urban farming activities. Organic fertilizer is beneficial as an effort to reduce waste’s negative impact to environment, and to minimize the use of chemical fertilizers. This research aimed to study the macro nutrient content of fruit peel based-liquid organic fertilizer (LOF) and its effect on the growth of Pakcoy (Brassica chinensis L.). Several fruit peels from household organic waste such as banana, mango, orange, dragon fruit, and papaya peels were used as material for LOF preparation. The research was conducted with completely randomized design (CRD) method with 5 treatments and 5 replications, namely the K- (without fertilizer, only water), K+ (0.2 mL commercial LOF), P1 (0.1 mL fruit peel based-LOF), P2 (0.2 mL fruit peel based-LOF) and P3 (0.4 mL fruit peel based- LOF) for 56 days of observation. The obtained data were analyzed using one-way ANOVA. Results showed that macro nutrients of fruit peel based LOF did not yet meet the quality standards set by the Ministry of Agriculture, and the dose of 0,4 ml fruit peels based-LOF showed the best effect to support the growth of the Pakcoy plant in all Pakcoy growth parameters. Keywords: Fruit Peels, eco enzyme, macro-nutrients, pakcoy

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