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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 100   101   102   103   104 
PERBANYAKAN TANAMAN WIEP {Grevillea papuana) SECARA KULTUR JARINGAN L Agus Sukamto
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i5.1193

Abstract

The population of wiep (Grevillea papuana) originated from Papua, decreased because of over cutting or over exploitation for timber, ornamental or medicine. Experiment was carried out to propagate wiep by using tissue culture method. Hypocotyls, cotyledons and nodes from seedlings grown in vitro, were used as explants. These explants were grown on Murashige & Skoog medium which macro and micronutrients were half strength, with or without hormones (BA 1 mg/1, BA 1 mg/1 + NAA 0,5 mg/1, BA 2 mg/1 + NAA 0,5 mg/1, BA 1 mg/1 + 2,4-D 0,5 mg/1, and BA 2 mg/1 + 2,4-D 0,5 mg/1).All explants did not produce callus on medium without hormones, but produced callus with hormones except BA treatment on cotyledon explants. Bigger callus were produced by explants that were treated by combination BA with NAA or 2,4-D compare to those treated by BA only. Nodes and hypocotyls could produce shoots, but cotyledons did not. Three kinds of explants did not produce any roots. Node is the best explants for producing shoots.Combination of BA 1 mg/1 and 2,4-D 0,5 mg/1 caused synergistic effect to stimulate shoot production of wiep hypocotyls explants.
PURIFIKASI DAN KARAKTERISASI ENZIM PEKTINASE DARI Aspergillus ustus BL5 Yopi Yopi; Nanik Rahmani; Ade Andriani; Fitria Dewi; Anja Meryandini
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.646

Abstract

Pectinase is an enzyme that could hydrolyze pectin into galacturonic acid. Natural pectinase was produced by microbes such as bacteria, yeast, fungi and Actinomycetes. Application of pectinase in industry were mainly in juice industry, textile, pulp, tea, cocoa and coffee fermentation. In this research, we conducted purification and characterization of pectinase produced by Aspergillus ustus BL5 in submerged fermentation using commercial pectin. The result showed that the optimum of pectinase production was reached at 120 hours fermentation process with specific activity 0.59 U/mg. The crude extract of pectinase was then concentrated using PEG 6000 and purified by Sephadex G-75 gel filtration chromatography. There were 2 fractions contained pectinase which the activity was 4.15 U/mg (pectinase A) and 3.3 U/mg (pectinase B), respectively. Compare to crude extract, the yield product of pectinase A and B increased 6.94 and 5.53 times, respectively. The purified pectinase A have optimum temperature at 50 oC and optimun pH at 5.
EVALUASI KARAKTER PEKA PANJANG HARI (PHOTOPERIOD) PADA TIGA GOLONGAN (Subspecies) PADI (Oryza sativa) SERTA PENGARUHNYA TERHADAP KARAKTER AGRONOMIS Tintin Suhartini
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1999

Abstract

Day length or photoperiod is a situation of replacement of light period to dark which able to control the flower initiation and also growth of crop. Rice is short day plant; if day length exceed the critical phase it will be late of flowering.The purpose of this experiment was to evaluate rice cultivars which differ in groups and agronomic characters to photoperiod.The experiment was done at two seasons in November 1997 and May 1998 at green house BB-BIOGEN Bogor. The total of 29 cultivars consist of 3 subspesies of javanica, indica and japonica type were used. The experiment was based on randomized block design with 4 replications. The result showed the local varieties were sensitive and javanica were medium, while improved varieties and japonica type were insensitive to photoperiod. The effect of seasons were significantly different to flowering days of local and javanica types, significantly and not significantly different to japonica, while not significantly different to improved varieties. The season has not significant effect to panicle length and unfilled grain per panicle characters for all cultivars type. Plant heigth and dry straw per hill were higher in rainy season, while fill grain per panicle, total grain per panicle and 1000 grain weight were higher in dry season. The results showed there have correlation between flowering days to all characters were observed, except number of tiller in rainy season was not correlated.
PENGARUH PEMUPUKAN NITROGEN, FOSFOR DAN KALIUM PADA PERTUMBUHAN TUNAS KANGKUNG (IPOMOEA AQUATICA FORST.) SAEFUDIN SAEFUDIN; R. SANTOSA
BERITA BIOLOGI Vol 3, No 5 (1986)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i5.1333

Abstract

SAEFUDIN & R. SANTOSA.1986.The effect of nitrogen, phosphorous and potassium fertilizers on the growth of Ipomoea aquatica Forst. Berita Biologi 3 (5): 214-218.Experiments to study the effect of nitrogen, phosphorous and potassium fertilizers on the growth of Ipomoea aquatica, a leafy vegetable, were carried out on four sites, i.e. in the villages of Teluk, Karangklesem, Purwokerto Kulon and Sumampir of the district of Banyumas respectively. The rates of application used were 0, 125, 250 and 375 kg of urea, triple superphosphate or potassium chloride per ha. The nitrogen fertilizer increased significantly the growth of Ipomoea aquatica with optimum rate 250 kg of urea per ha. Potassium chloride increased slightly the stem elongation, but not the vegetable yield, while the phosphorous fertilizer did not show any significant effect.Among sites, the highest yield was obtained from the plot in the village of Teluk, followed by Karangklesem, Purwokerto Kulon and Sumampir in decreasing order.
NEMATODA PADA TIKUS SUKU MURIDAE DAN POLA INFEKSINYA DITAMAN NASIONAL LORE LINDU, SULAWESI TENGAH Endang Purwaningsih; Kartika Dewi
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.831

Abstract

The study of the patterns of nematode infection on rodents in Lore Lindu, Central Sulawesi was carried out. A total of 52 rodents were examined. Of these rodents 37 (71.15 per cent) were found to harbour nematode parasites and 15 (28.85 per cent) were free.There were found 10 species of nematode: Subulura andersoni, Syphacia muris, Heterakis spumosa, Cyclodontostomum purvisi,Hepatojarakus malayae, Molinacuaria indonesiensis, Protospirura muris,Rictularia tani, Trichuris muris dan Nippostrongylus muris that found in intestine, stomach, cecum and liver.The kind of infections were single (17.31 per cent) and association (53.84 per cent).The highest prevalence was 5. andersoni (44.23 per cent). Each of species nematodes had a specific habitat in their hosts. The greatest number of habitat that species of nematodes found was stomach (all of the species nematodes were found at the stomach).
Pellacalyx Symphiodiscus STAFP FROM LONG BAGUN, MAHAKAM HULU: MORPHOLOGICAL CHARACTERIZATION AND ITS DISTRIBUTION Inggit Puji Astuti; Ratna Susandarini; Rismita Sari
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2650

Abstract

Pellacalyx is one of the genera in Rhizophoraceae, distributed from Myanmar, South China, Indonesia, Malaysia and the Philippines. In Indonesia, Pellacalyx can be found in the island of Sumatra, Kalimantan and Sulawesi. Eight species of Pellacalyx have been found  in Indonesia, four of which them were from Kalimantan, i.e. P. axillaris, P. cristatus, P. lobbii and P. symphiodiscus. These four species generally grow in the primary and secondary forest, from lowland to an altitude of 1300 m above sea level. Information of Pellacalyx in Indonesia is still very limited , and rarely found in the wild. The last information of Pellacalyx found in West Sumatra was reported by an expedition team of the Center for Plant Conservation Bogor Botanical Garden in 2006.  The Expert Team of the Khatulistiwa Expedition in Long Bagun, West Kutai, East Kalimantan on May 26–June 2, 2012 found a species of Pellacalyx. Morphological characterization was then conducted based on the herbarium specimen for identification. The identification was carried out in Bogor Botanic Gardens, Center for Biological Research, Indonesian Institute of Sciences and The National Herbarium Leiden, Netherland. The results show that Pellacalyx specimen from Long Bagun is Pellacalyx symphiodiscus. 
PEMBAHASAN Bungaran Saragih
BERITA BIOLOGI Vol 5, No 5 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i5.1473

Abstract

Menurut pendapat saya seharusnya NTT bisa lebih makmur daripada yang ada sekarang ini, tetapi sangat sayang sekali rupanya sumber daya alam yang sangat penting di sana itu tidak digunakan secara baik atau tidak dikelola secara baik. Oleh karena itu tidak bisa menyumbang untuk Pembangunan Daerah padahal altematif yang lain barangkali sangat sulit untuk ditawarkan. Kalau saya dengar tadi penjelasan, masalah yang pokok dari tanaman Cendana ini adalah bukan pada masalah teknis biologis; masalahnya adalah policy, kebijakan. Pemerintah Daerah melanjutkan apa yang telah dilakukan oleh Pemerintahan Kolonialdan tidak memperbaikinya. Apa yang dibuat Pemerintahan Kolonial sudah bagus; bagus untuk mereka, bagus untuk kepentingan negara yang menjajah. Tetapi setelah kita merdeka 'konteks' tidak kita pelajari, kita lakukan hal yang sama dan akibatnya seperti sekarang. Kalau tadi Bapak Kepala Dinas Kehutanan Propinsi mengatakan "semuanya tidak ada lagi biaya dinas", saya melihat dengan begitu cemasnya, jangan-jangan Cendana sudah akan punah. Seharusnya NTT bisa memiliki monopoli internasional terhadap cendana, tetapi itu tidak dimungkinkan lagi dan orang (negara) lain sudah mulai mencari alternatif, menanam. Cendana India yang asalnya dari Timor sekarang sudah lebih terkenal dari Cendana Timor sendiri, seperti yang dikatakan Bapak Hartono (Pembicara, Pengusaha dari PT Tropical Oil, Red.). Intinya adalah bahwa komoditi itu terlalu diregulasi oleh Pemerintah; dan tidak hanya diregulasi tapi dimonopoli, dan merampas hak rakyat untuk melakukan bisnis ini.
TATA AIR PODOLANDAK (Baccaurea sp.), SPESIES JARANG KAWASAN HUTAN TROPIK TERDEGRADASI AKIBAT PENAMBANGAN EMAS DI BOJONGPARI, JAMPANG, PELABUHAN RATU, JAWA BARAT B. P. Naiola
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1157

Abstract

Forest exploitation activities always brings some environmental problems such as reduction in individual plant or species biodiversity and some physical deterioration which lead to microclimate change.This study presenting the results of measurement of plant-water relations against a shrub plant (Sundanese) podolakan (Baccaurea sp.),a rare species in a degraded tropical forest land under gold mining pressure in Jampang,Pelabuhan Ratu, West Java.Results shows that no significant differences between plants growing in the degraded and non-degraded (natural) sites in their water relations aspects emphasized on water potential components i.e. total water potential (¥, -MPa): 0,48 and 0,38, osmotic potential (¥„, -MPa):1,64 and 1,44 and turgor pressure (y/p, MPa): 1,16 and 1,06 respectively. The values of their fat turgorloss point showed no significant different either between degraded and natural sites, i.e. -1,93 MPa and -1,43 MPa.Interestingly, in comparison with the dominant species i.e. puspa (Schima wallichii (DC) Korth.) in the non-degraded site, results shows a significant different between these two degraded forest species in their ¥„ (0,01>P>0,001), where there was better developed in the rare species (-1,44 MPa) than the dominant ones (-1,29 MPa). While in the degraded site, there was also a significant different (P<0,001),where the rare species had better development (-1,64 MPa) than the dominant (-0,67 MPa). It seems that the dominant spesies in the degraded site underwent more environmental (water) stress than the rare species as shown by more negative of its Y (-0,82 MPa),while rare species was only -0.48 MPa. Water potentials at turgor loss points were not significantly different between Baccaurea sp. in degraded and natural sites.It is suggested that even though as a rare species, Baccaurea sp.retained better water status. This is maybe due to its position in the forest as lower strata plants, thus received less effect of climatic stress in upper canopy such as more light intensity and bigger wind flow. Hence, in natural reclamation, plant species like Baccaurea sp. may have better chance to role as pioneering species. No significant differences in f at f(turgor loss points) between the degraded and natural sites (-1..93 and -1,43 MPa respectively). It is suggested that water is not a limiting factor for the plants in degraded tropical forest site under gold mining pressure.
INTERAKSI GALUR × LINGKUNGAN, POTENSI HASIL DAN STABILITAS HASIL GALUR HARAPAN KEDELAI {Glycine max (L.) Merr.} HITAM M Muchlish Adie; Ayda Krisnawati; GWA Susanto
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.520

Abstract

Recent demand of black soybean {Glycine max (L.) Merr.} seed has been rising for industrial raw material. A total of five promising lines of black soybean (9837/K-D-8-185, 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211) were evaluated for the potential yield at 18 soybean production centrals in West Java, East Java, Bali and West Nusa Tenggara, from 2004 until 2006..Variety of Cikuray (black soybean), Wilis (famous variety with high yield) and Burangrang (large seed size and short maturation day) were used as check varieties.The field experimental design for each location was completely randomized design with four replicates.The treatment consist of eight lines/variety with 2,4 m × 4,5 m plot size, 40 cm × 15 cm plant distance, two plants/hill.Fertilizer of 50 kg Urea, 100 kg SP36 and 75 kg KCl per ha were applied before sowing time. Weed, insect and disease were controlled intensively.Stability analysis was according to Eberhart and Russell (1966), and adaptability was by Finlay dan Wilkinson (1963). Location, genotype and interaction between 2genotype x environment (G × L) were significant for seed yield. Estimate of environment variance component (d L = 0.0604) was the 2 2 biggest one, and followed by error variance value (d E = 0.0470), genotype variance (d G = 0.0258) and the smallest was estimate of 2 interaction variance G × L (d GL = 0.0225).Significant interaction of G × L showed that each promising lines have specific reaction to certain location quality. Five promising lines were able to produce 2.44 until 2.51 t/ha, or 17% higher than black soybean variety of Cikuray (2.03 t/ha); or 10% higher than Burangrang and 3% more higher than Wilis. Genotype of 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211 were indentified stable. Genotype of 9837/K-D-3-185-195, W/9837-D-6-220, 9837/K-D-3-185-82 and 9837/W-D-5-211 were promising to be high yielded and adaptable across location; on the contrary, genotype of 9837/K-D-8-185 was more adaptable in optimal location.
SIDIK JARI DNA PLASMA NUTFAH PADI LOKAL MENGGUNAKAN MARKA MOLEKULER SPESIFIK UNTUK SIFAT PADI BERAS MERAH Dwinita W Utami; Aderahma Ilhami; Ida Hanarida
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1966

Abstract

Red rice is one corp that has genetic variation both based upon phenotype and genotype evaluation using moleculer markers for specific gene. A research objective is to identify genetic diversity using red rice specific molecular markers. Forty accessions of Indonesian local rice included 9 accessions of red rice germplasm were used as genetic materials for this research. Specific molecular markers (RC3, RC9 and RC12) for a pigment color found in rice seed were designed based on alignment sequence analysis to rc-bHLH gene, a transcription factor for prothocyanidine protein. Then these markers were used to analyze the genetic variation of red rice. Cluster analysis and association test between phenotype and genotype performances were analyzed by TASSEL 2.1 software program. Results showed that red rice accessions distributed into 2 cluster. One cluster is more closely related to white rice than the other. The association test showed RC12 is the most significant marker in association with the red rice trait: red pericarp and white aleuron. These phenotype variations were found in Cempo merah, one accession originated from Yogyakarta and Pulut mandoti, one accession from South of Sulawesi.

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