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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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KERAGAMAN GENETIK BEBERAPA KLON DURIAN (Durio zibethinus Murray) ASAL JAWA BARAT BERDASARKAN SIDIK RANDOM AMPLIFIED POLIMORPHIC DNA Yulita, Kusumadewi Sri; Murnianjari, Muna
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Durian (Durio zibethinus) is one of the most popular tropical fruits in SE Asia. Indonesia has several local clones that have not yet been widely introduced to local fruit markets. This present study aimed to assess genetic diversity of 17 clones of durian from West Java based on RAPD fingerprints. Thirty RAPDs primers were initially screened and four were selected for the analysis.These four primers (OPA 13, OPD 8, OPN 6 and OPA 18) generated 63 scorable bands to which 100% of them were polymorphic.OPA-13 at 700bp was exclusively possessed by Tambleg clone and other bands were shared among the other clones. Clustering analysis was performed based on RAPD profiles using the UPGMA method. The range of genetic similarity value among genotypes was 0.15-0.73 suggesting high genetic variation among them. Results from genetic diversity analysis based on plant propagation system showed a higher genetic diversity value in occulating (87.30%) plants than that of grafting (60.32%).

AKTIVITAS ENZIM 2,4-D MONOOKSIGENASE DARI BERBAGAI MIKROBA [2,4-D monooxygenase Activity of Some Microorganisms] Sulistinah, Nunik
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Nine cultures both from fungi and bacteria have been selected for testing their 2,4-D monooxygenase activity to degrade 2,4-Dtohlorophenoxyacetic acid (2,4-D). The results showed that all the cultures which were tested grows at 1000 ppm 2,4-D. Three cultures (Trichoderma viride. Asperoillus niqer and Isolat E (unidentified)) of the nine cultures are able to grow at 4000 ppm. T. viride grows well on Minimal Basal Media which contained glucose and 2000 ppm 2,4-D and produced the highest biomass (0.8660 g/l) than the others. The biomass of T. viride grew on MBM (without glucose) and added with 2000 ppm 2,4-D is 0,6520g/l. This indicated that the culture is tolerant to 2,4-D and able to use 2,4-D compound as energy and carbon sources for its growth. But we failed to prove the 2,4-D monooxygenase activity of supernatant of T. viride by measuring the changing of pH-value in the 2,4-D breakdown reaction.

KEANEKARAGAMAN JENIS TUMBUHAN PEMAKAN SERANGGA DAN LAJU FOTOSINTESISNYA DI PULAU NATUNA [Diversity of Insectivorous Plants and Its Photosynthetic Rate in Natuna Island] Mansur, Muhammad
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Studies of insectivorous plants diversity and photosynthesis rate of pitcher plants in Natuna Island was conducted on August 2011, Survey in eleven villages conducted to determine the species found in the visited sites, Bioscientific Portable LCI ADC Photosynthesis System used for measurement of pitcher plants photosynthesic rate, The results shows that there are three families of insectivorous plants founded in study sites, namely Nepenthaceae, Droseraceae and Lentibulariaceae, Six species of Nepenthaceae were found (four species and two natural hybrids) i,e; N. gracilis, N. ampullaria, N. rafflesiana, N. reinwardtiana, N. x hookeriana and N. x trichocarpa. While from family Droseraceae and Lentibulariaceae were found only one species respectively, which is Drosera burmanni and Utricularia bifida. Nepenthes reinwardtiana has the highest rate of photosynthesis namely 21,05 Âµmol/m /s, followed by N. gracilis (17,66 Âµmol/m /s), N. rafflesiana (16,6 Âµmol/m /s), N. x hookeriana (15,84 Âµmol/m /s) and N. ampullaria (9,96 Âµmol/m /s).

KARAKTERISASI PISANG REJANG TETRAPLOID HASIL INDUKSI DENGAN ORYZALIN [Characterization of tetraploid Pisang Rejang induced by oryzalin] Poerba, Yuyu Suryasari; Handayani, T; Witjaksono, Witjaksono
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Triploid banana cultivar is the most desirable cultivar in banana industry because of its higher yield compared to its diploid cultivar. Â The Â triploid cultivar can be produced by crossing tetraploid with diploid cultivar. Â However, tetraploid banana cultivar is rarely existed naturally. Â Induced tetraploid of Pisang Rejang was produced using oryzalin. The present research was conducted to characterize tetraploid Pisang Rejang (Musa acuminata, AAAA genome) induced by in-vitro oryzalin treatment from diploid Pisang Rejang. Ploidy level, molecular and morphotaxonomic characters were observed. Â Ploidy identification of induced Pisang Rejang was conducted using Flowcytometer. Â Molecular characterization was done using RAPD and ISSR markers. Morphology characters were observed based on UPOV (2010). The results showed that tetraploid plants have similar genetic properties with their diploid controls as shown by genetic identity of 0.9901 â 0.9935. The tetraploids were differed from their diploid plants in plan habit and diameter of fruit. Â The tetraploid plants produce fewer suckers, drooping leaves and broader fruits compared to its diploid control.

SEBARAN KEPITING MANGROVE (CRUSTACEA: DECAPODA) YANG TERDAFTAR DI KOLEKSI RUJUKAN PUSAT PENELITIAN OSEANOGRAFI-LIPI 1960-1970 [The Mangrove Crabs (Crustacea: Decapoda) recorded in refference collection of Research Centre for Oceanografi-Indonesian Insitute of Sciences 1960-1970] Pratiwi, Rianta; Rahmat, Rahmat
BERITA BIOLOGI Vol 14, No 2 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Brachyura is a group of Crustaceans species often found in mangrove areas. Mangrove crabs in this study were from mangrove areas found in almost all Indonesian waters that were stored in the Reference Collection of Marine Biota Division, Research Center for Oceanography, Indonesian Institute of Sciences (LIPI) from 1960 to 1970. The aim of this study was to assess the presence and distribution of mangrove crabs from the waters of Indonesia as a search and as a reference collection from 1960 to 1970.A total of 359spesimens were observed, consisted of 54 species, 22 genera, and seven families. The data were stored in a database system to perform spatial information analisys.

NILAI KEPENTINGAN BUDAYA KEANEKARAGAMAN JENIS TUMBUHAN BERGUNADI HUTAN DATARAN RENDAH BODOGOL, SUKABUMI, JAWA BARAT [Index Cultural Significance of Useful Plants Diversity in Bodogol Lowland Forest, Sukabumi, West Java] Rahayu, Mulyati; Purwanto, Y; Susiarti, Siti
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Index Cultural Significance (ICS) of useful plants diversity in ethnobotanical study is an importance step, such as activity strategy which have subsystem aspect and traditional classification. Ethnobotany study in Bodogol lowland forest, West Java recorded more than 200 useful plants species within 3 times visits in 2009 to 2010. Data collection was done by âwalk in the woodâ,interview method, direct observation in permanent plots and estimation of Index Cultural Significance (ICS) form.Result of the study indicated that indigenous people around Bodogol lowland forest has good knowledge about biodiversity around them. Based on interview method, this study showed that indexs cultural significance of useful plants in this area was in the range of 0.5 and 86. Kawung {Arenga pinnata (Wurmb) Merr.} has highest ICS whilst Acung leutik (Arisaema filiforme Bl.) is the lowest.

CATATAN BEBERAPA JAMUR MAKRO DARI PULAU ENGGANO: DIVERSITAS DAN POTENSINYA [Notes on Some Macro Fungi From Enggano Island: Diversity and its Potency] Retnowati, Atik; Susan, Dewi
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2939

A Thirty one species of macro fungi which belong to phylum Basidiomycota and Ascomycota were found from three villages in Enggano Island. Among those, nine species are new records for Sumatera, namely Phellinus gilvus, Fomitella supina, Flaviporus liebmannii, Coriolopsis polyzona, Flabellophora sp., Trichaptum byssogenum, Stecherrinum sp., Stereum cf. pergameneum dan Trametes cf. villosa, whilst other two species, namely Cookeina cremeirosea and Fomitella supina are new for Indonesia. One species, Phellinus gilvus, has been known as a harmful parasite on rubber plantation. Six are recognized as edible mushrooms, namely Pleurotus ostreatus, Volvariella volvacea, Schizophyllum commune, Auricularia auricula-judae, Lentinus sajor-caju, and Panus neostrigosus. Two species, Favolus grammocephalus and Panus neostrigosus are therapeutic agents, and two others, Earliella scabrosa and Pycnoporus sanguineus are potential species as color degradation agents.

THE STATUS OF Trichosanthes anguina L. (CUCURBITACEAE) ) [Status Trichosanthes anguina L. (Cucurbitaceae)] Rugayah, Rugayah
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i3.1066

Perbedaan pendapat tentang status kedudukan taksonomi Trichosanthes anguina sebagai jenis yang berbeda dengan T. cucumerina,maupun sebagai anak jenis T. cucumerina subsp. anguina atau varietas T. cucumerina var. anguina memerlukan data pendukung.Anatomi daun dengan sayatan paradermal menunjukkan bahwa keduanya mempunyai bentuk stomata dan sel epidermis yang sama,tetapi berbeda hanya pada ukurannya. Stomata Trichosanthes anguina berukuran 15-17.5 x 10-12.5 urn dan sel epidermis 22.5-31.25 x 12.5-20 m, sedangkan Trichosanthes cucumerina mempunyai stomata 10-12.5 x 8.75-12.5 um dan epidermis sel 15-25 x 10-15 um. Data tersebut mendukung dalam menetapkan T.anguina sebagai T. cucumerina var.anguina.

IDENTIFIKASI Lernaea sp. YANG MENGINFEKSI IKAN ARWANA IRIAN {{Scleropagesjardinii (Saville-Kent, 1892)} DI MERAUKE, JAKARTA, BOGOR DAN DEPOK Shatrie, Dikry N; Imamudin, Kurniasih; Nurcahyo, Wisnu; Triyanto, Triyanto
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Lemaea spp. is very important fish ectoparasites because it spreads all over the world and its economic importance has increased due to numerous epizootics occurrence among the most important farmed fish; and has wide host range. Lemaea sp. has been infected not only the family Cyprinidae and Characidae but also in Osteoglossidae family, such as Red Arwana [Scleropagesformosus) in Malaysia and Arwana fish {Scleropagesjardinii (Saville-Kent, 1892)) in Irian.The objective of this study was to identity Lernaea sp. often attacked Arwana irian fish as a precautionary measure the spread of infection, especially on Arwana irian fish. Arwana irian fish were collected from Merauke, Jakarta, Bogor and Depok, 10 infected fish per area. Found Lernaea sp. fixed in glutaraldehyde for morphology study. Based on morphological charactheristic, there were six different Lemaea sp. attacked Arwana irian fish. They are Lemaea cyprinacea, L. devastatrix, L. lophiara, L.oryzophila, L. papuensis and Lemaea n sp. Lemaea n sp. was considered as a new species due to different variation of holdfast organ.

UJI PALATABILITAS TEPUNG BUNGKIL KELAPA SAWIT YANG DIHIDROLISIS DENGAN ENZIM RUMEN DAN EFEK TERHADAP RESPON PERTUMBUHAN BENIH IKAN PATIN SIAM (Pangasius hypophthalmus Sauvage) [The Palatability Test of Palm Kernel Meal Hydrolyzed by Rumen Enzyme and Growth Reponse of Seed Catfish (Pangasius hypophthalmus Sauvage)] Pamungkas, Wahyu
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.644

The quality of feed is not only determined by the nutrient content and digestibility of the feed or feed ingredients, but also determined by the palatability of the feed. Palatability is one of important factor in the preparation of rations, because palatability affects the amount of feed intake. The purpose of the study was to determine the effect of feed commercial mixed with palm kernel meal (PKM) that was hydrolyzed by sheep rumen liquor enzyme to palatability of feed by measuring total feed consumption and growth responses of seed catfish (Pangasius hypopthalmus Sauvage). The feeds used in the study were commercial feed added hydrolyzed palm kernel meal (PKMe) and unhydrolized palm kernel meal (PKM). The treatments were as follows A). Commercial feed, B). 30% + 70% BKSecommercial feed30% PKMe + 70% commercial feed, C). 40% PKMe + 60% commercial feed, D). 30% PKM + 70% commercial feed, E). 40% PKM+ 60% commercial feed. Ten fishes with wight around 20 g were used in the trial and held in 80 l tanks. A completely randomized experimental design consisted of 1 variable and triplicates were selected. The result of the experiment showed that feed consumption was not significantly different among the treatment. It was showed that the test diet have a good palatability. The result of feed consumption, survival rate and growth responses observation showed that it was not significantly different among the treatments (P>0.05). Result of digestibility analysis showed that diet A and B have digestibility coefficient higher than diet C and D. Based on the evaluation in those parameters it was concluded that palm kernel meal that has been hydrolized by sheep rumen liquor enzyme have a good palatability and can be used as feed for seed catfish (Pangasius hypophthalmus Sauvage) with addition of up to 40% in the feed.

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