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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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RESPON PERTUMBUHAN DAN KETERGANTUNGAN Albizzia saponaria (LOUR.) MIQ TERHADAP INOKULASI FUNGI MIKORIZA ARBUSKULA LOKAL SULAWESI TENGGARA PADA MEDIA TANAH PASCA TAMBANG NIKEL Tuheteru, Faisal Danu; Husna, Husna; Arif, Asrianti
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1917

This study that so far has not been conducted yet by others, regarding the effect of a local AMF species from Southeast Sulawesi on the growth of Albizzia saponaria (Lour.) Miq. The effect of AMF to the plant growth depends on the compatibility between host plant and fungi species which was controlled by genotypes of the both symbionts. The aim of this research is to know the growth respons and dependency of A. saponaria to this local AMF grown in post-nickel mining soil. This research was conducted in the Screen House of Forestry Department, Faculty of Agriculture-Haluoleo University since June to September 2010. A completely randomized design with three replications was used. The treatments were no inoculation of AMF (A), inoculated with -1 -1AMF Mycofer inoculum 5 g polybag (B), and inoculated with local AMF inoculum 10 g polybag (C). The seedling parameter observed were height, diameter, number of leaf, total biomass, number of nodule, percentage of root colonization and relative -1 mycorrhizal dependency (RMD). Results showed the increasing growth and biomass with the application of AMF 10 g polybag (C). There was a positive-strongest correlation value (P<0.01) between percentage of root colonization to all of seedling growth parameters, except to the diameter which was only strong correlated (P<0.05). A. saponaria seedling has high dependency level on local AMF for their survival in heavy metal contaminant soil.

PENINGKATAN PERFORMA REPRODUKSI IKAN PATIN SIAM (Pangasianodon hypophthalmus) PADA MUSIM KEMARAU MELALUI INDUKSI HORMONAL [Reproduction Improvement on Female Striped Catfish Pangasianodon hypophthalmus at Dry Season By Hormonal Induction] Tahapari, Evi; Dewi, Raden Roro Sri Pudji Sinarni
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Striped catfish (Pangasianodon hypophthalmus) spawning success is strongly influenced by environment.Striped catfish spawning generally occurs during the rainy season while in the dry season is difficult to find mature female. This research was conducted to improve reproduction performance of female striped catfish during dry season through combination injection of hormone pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG).The treatments were A : control (no hormone injections), B : injection of PMSG 10 IU/kg + HCG 10 IU/kg and C : injection of PMSG 20 IU/kg + HCG 10 IU/kg.Injections were done at interval of two weeks as many as six times. The research was conducted during dry season in June-October 2012 with rainfall 0.20- 14.40 mm/years. The results showed combination injection of PMSG + HCG could improve female reproduction performance. The best treatment was shown on C treatment with values of OSI (ovi somatic index), fecundity, fertilization rate, hatching rate and larva production was 12.18 %, 523 000 eggs/female, 79.65 %, 69.93 % and 290 000 larvae/female respectively. As for control treatment (A) the value of OSI, fecundity, fertilization rate, hatching rate and larval production, was 6.45 %, 197 000 eggs/female, 48.55 %, 35.59 % and 34 000 larvae/female, respectively

IDENTIFIKASI SECARA CEPAT BAHAN BIOAKTIF PADA TUMNUHAN DI LAPANGAN Chairul, Chairul
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

New drug discovery from natural products (bio-prospecting) is not an easy works and takes time, big budget, human resources etc. some important approaches must be taken in order to get success. A preliminary observation of biologycally active components is impotant approach in order that more selective in collecting research materials in the field. This approach is purposed to pacilitate (make easier) the next step research process later in the laboratory level. Several Methods of preliminary observaton of biologyvcally active components had been carried out in the field, but the general guideline of fast observation had been used e.g. 1). Etno-botany (Etnomedicine and Etnopharmacology), 2). Chemotaxonomy, 3). Organoleptic, 4). Chemical (reagent kit) and 5).Simple bioassay in the field (fish posion test, insecticide). Besides that collecting the herbarium specimen as well as research materials for any scienfitic and laboratory works. This paper described these approaches in order to make useful methods to researchers, who go to the field and they could collect more selective research materials before bring them to laboratory.

ANALISIS VEGETASI DAN DEGRADASI JENIS TUMBUHAN HUT AN GAMBUT SETELAH KEBAKARAN DIKAWASAN TAMAN NASIONAL TANJUNG PUTING KALIMANTAN TENGAH Yusuf, Razali
BERITA BIOLOGI Vol 5, No 3 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This report deals with the result of an ecological study with reference to disclose the effect of wild fire and human interference on the peat forest deterioration and plant species degradation in Tanjung Harapan Camp, Tanjung Putting National Park, Central Kalimantan. By using quadrate method, a one-hectare both fired and unfired (natural) plots were compared.Within the fired-plots, only 19 plant species were recorded belongs to 16 genera and 12 families, while in the unfired plots, 50 plant species belongs to 34 genera and 23 families were found. The dominant species in both plots was component of pioneering species;Macaranga hypoleuca was one of the dominant.The result of successions after one year of fire showed that Dillenia suffruticosa and Baccaurea bracteata became the most frequent species found with the largest area of distribution.

DIVERSITAS DAN PROFIL METABOLIT SEKUNDER JAMUR ENDOFIT YANG DIISOLASI DARI TUMBUHAN GAMBIR (Uncaria gambler) SERTA AKTIVITAS BIOLOGISNYA SEBAGAI ANTIBAKTERI Jamal, Yuliasri; Ilyas, Muhamad; Kanti, Atit; Agusta, Andria
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Five kinds of endophytic filamentous fungi i.e. Pestalotiopsis sp. GNC, Fusarium sp. GNC-A, Fusahum sp. GNC-B, Pestalotiopsis sp. GUC and Fusarium sp. GUC were isolated from surface sterilized of steems of gambir nasi and gambir udang (Uncaria gambier Roxb.) collected from Rengat, Riau Province,Indonesia. Identification of the endophytic fungi were done through morphologically observations and chemotaxonomically approach. In an antibacterial assays, the ethyl acetate extract of the Pestalotiopsis sp.GNC cultures showed their activity against pathogenic bacteria, Eschericia coli, Staphylococcus aureus and Bacillus sublilis.Beside the above three pathogenic bacteria, the ethyl acetate extract of Fusarium sp. GNC-B culture was also active against

KERAGAAN HIBRIDAINTRA SPESIFIK DARIEMPAT STRAIN IKAN NILA (Oreochromis niloticus) DIKARAMBA JARING APUNG, DANAU LIDO, BOGOR Mahardhika, Prana; Gustiano, Rudhy; Soelistyowati, Dinar Tri; Ath-thar, MH Fariduddin
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The objectives of the study was to evaluate hybrid performance of sixteen reciprocal cross resulted from three strains (Red NIFI,Nirwana, BEST) and one population (Lido Lake population) of nile tilapi. The study was conducted in experimental floating netcages in Lido Lake. Fish of 25 days old ranged 0,4 Â± 0.07 g were used in the experiment. Fish were reared in floating net cages sized 2x1x1 m for each cross with the density of 50 fish/cage for eight weeks (56 days). Feed were given 10% of body weight with frequency three times a day. The results showed that the highest average larvae production was 1216 from Nirwana female crossed with four different male. Growth rate of hybrids were higher than the parents (P < 0.05) and the highest was .ed NIFI x c? BEST (6,440Â±0,0712% ).The highest survival rate was $ Red Lido x S BEST hybrid (77.0 Â±2,78%")). For heterosis, Specific Growth Rate (SGR) was 5.8188% on hybrid between Nirwana and Red Lido. While for survival rate was 15.5888% on Nirwana x BEST hybrid. Crossing between fenotipe of light color background (red) and dark color background (black) showed that red color was dominant to the black one. Pleiotric effect of color gen was detected on the SGR and survival rate.

Di Sela-Sela Laboratorium dan Plot Eksperimen MENGENAL HPLC: PERANANNYA DALAM ANALISA DAN PROSES ISOLASI BAHAN KIMIA ALAM Murningsih, Tri; Chairul, Chairul
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The development of method to explore natural chemical product initially from plants were started from the beginning and generously from time to time from the conventional ones until sophisticated equipments.The value of plants as food,cosmetic and medicines resources needs specific instruments and methods for determining the quantity as well as quality of the materials in plants.This paper describe in detail one of the sophisticated equipments commonly used for analyzing and isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC).

KARAKTERISASI BAKTERI PENGHASIL a-AMILASE DAN IDENTIFIKASI ISOLAT C2 YANG DIISOLASI DARI TERASI CURAH SAMARINDA, KALMANTAN TIMUR [Characterization bacteria Producing a- amylase and Identification of Strains C2 Isolated from bulk shrimp-paste in Samarinda, East Kalimantan] Soeka, Yati Sodaryati
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Enzymes a-amylase is one of the enzymes used in process of starch degradation. This present study was aimed to characterize and identify of a-amylase producing strain isolated from bulk shrimp-paste in Samarinda, East Kalimantan was carried out. An experiment was conducted to examine influences of incubation, carbon source, substrate concentration temperature for incubation, pH of media, and addition of metal ions. Identification of strain C2 was carried out by using Wizard Genomic DNA Purification Kit (Promega). The result showed that optimum activity of a-amylase from C2 after six days incubation was 18.93 U/mL. Tests on the type of substrate , soluble starch was the best source to produce a- amylase (14.51 U/mL). However, at concentration of 2 % and incubation temperature at 40Â°C, enzymatic activity was decreased to 12.56 U/mL and 12.79 U/mL, respectively within residual activity of 74.75%. The enzyme activity was 16.43 U/mL and its residual activity was 39.14 % when it was assayed at pH 8.5. Addition of metal ions in the form of divalent and monovalent cations (1 mM) showed that the enzyme could be activated by ion Ca2+ while ion Cu2+, Co2+, Mg2+, Zn2+ , Na+ Â decrease the activity of the enzyme. Identification of strain C2 using molecular characterization demonstrated that partial sequences of 16S rDNA reffered to as Bacillus subtilis.

Stappia aggregata Gl DAN Alteromonas sp.G2 BAKTERIPENDEGRADASI PHENANTRENE YANG DIISOLASIDARI LINGKUNGAN LAUT Supriyati, Dyah
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Stappia aggregata Gl and Alteromonas sp. G2 are marine bacteria isolated from Kepulauan Seribu waters, Jakarta. These bacteria were isolated using ONR7a media, and forming clear zone surrounding grown colonies after 48 hours, and able to grow on phenantrene as the sole carbon sources. After 4 hours incubation phnenantrene was degraded, and almost 12 hours about 60% of phenantrene was converted. The two strains performed similar biodegradation pattern, but different in growth characteristic.Bacteria Stappia aggregata Gl and Alteromonas sp. G2 grew optimum at 30Â°C, pH 8 and 3% salinity.

DINAMIKA POPULASI MIKROBA BENTUK FILAMEN PADA PROSES ANAEROBIK-AEROBIK UNTUK PENAMBATAN POSPAT DENGAN MIKROBA [Dynamic Population of Filamentous Organism in Anaerobic-Aerobic Process for Enhanced Biological Phosphate Removal Process] Sudiana, I Made
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1252

Filamentous bulking is commonly observed in activated sludge treatment plant. The present study is intended to study the dynamic of filamentous organism in enhanced biological phosphorous removal. An anaerobic-aerobic sequential batch reactor was operated at 6 cycles per day with anaerobic phase for 55 minutes, aerobic phase for 135 minutes and settling phase for 60 minutes. Population of filamentous organism were counted and identified.Fluorescence in situ hybridization (FISH) with oligonucleotide probe specific for the alpha, beta, gamma sub class Proteobacteria and gram positive high GC DNA content organism was done to identify in-situ community of filamentous organism in the activated sludge. Them were 4 major filamentous organisms namely Type 021N, 0041, Micmthrix oarvicella and Haliscomenobacter hydrosis, in which type 021N was always dominant. Deterioration of enhanced biological phosphorous removal process was observed when filamentous organism outgrew. Probing technique with oligonucleotide probe revealed that filamentous organism mostly belong to the gamma sub class of Proteobacteria.

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