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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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PEMANFAATAN BERBAGAI SENYAWA NITRIL DAN PRODUK DEGRADASINYA SEBAGAI SUBSTRAT UNTUK PERTUMBUHAN ISOLAT BAKTERI TP Supriyati, Dyah; Sunarko, Bambang
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Our experiments showed that bacterial isolate TP was able to grow on acetonitrile, butyronitrile and propionitrile as sole sources of carbon, energy and nitrogen, but not on acrylonitrile dan benzonitrile. Besides on nitriles, isolate TP could grow on acetamide,propionamide, benzamide and nicotinamide, but not on acrylamide. However, none of the tested carboxylic acids could be used as growth subtrate for bacterial isolate TP. The best growth substrates of isolate TP were butyronitrile (CH3-CH2-CH2-CN) and propionamide (CH3-CH2-CONH2). When isolate TP grew on butyronitrile, the highest biomass concentration, the doubling-time (td), and the specific growth rate1(n) were 8.99 gram cell dry weight/liter, 4.8 h and 0.144 h , and when grew on propionamide were 4.57 gram cell dry weight/liter, 5.7 h and10.122 h", respectively.

EFEKTIVITAS EKSTRAK BIJI PICUNG (Pangium edule Reinw.) TERHADAP MORTALITAS KEONG MAS (Pomacea canaliculate Lamck.) Yuningsih, Yuningsih; Kartina, Gina
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This research attempted to substitute commercial with botanical molluscicide i.e. picung (Pangium edule) seed. The trial of picung seed water extract (PWE) was conducted in 3 groups of gold apple snails (Pomacea canaliculata) - (GAS).Group 1 with body weight (bw) was 4 to 5 grams, Group 2 was 10 to 12 grams and Group 3 was blank. Both group (1 and 2) were soaked in 4 regimes of cyanide concentration from PWE: 100, 50, 25 and 12.5 /tg/mi and put 10 snails for each concentration. The observation on their mortality was conducted for 24 hours. The result showed that PWE killed 100% of GAS (< 1 hour) with concentration in 50 Âµg/ml and 25 ÃÂµ g/ml cyanide in Group 1 and Group 2, respectively, with clinical sign is the production of excessive mucous as criteria of molluscicide effect thus picung seed is quite significant as botanical molluscicide.

PENGARUH PADAT TEBAR IKAN KOAN {Ctenopharyngodon idella) TERHADAP LAJU PERAMBAHAN DAN LUAS TUTUPAN ECENG GONDOK (Eichornia crassipes) DI DANAU LIMBOTO, GORONTALO Krismono, Krismono; Rahardjo, MF; Harris, E; Kartamihardja, ES
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Water hyacinth {Eichhornia crassipes) in Lake Limboto was covering the area about 40 to 60% in 2008. It caused a lot of problems on water transportation, fishing activities, reducing water quantity and quality. To manage the number of water hyacinth population, biological control use omnivorous species and such as grasss carp (Ctenopharingodon idella) is a promosing alternative. In this study, effect of different stocking density of grass carp on the population of water hyacinth was observed. The result showed that 200 fish was the most effective compare to other stocking density and enabled to reducing the covering area up to 10 to 20%.

IMPACTS OF DRY SEASON AND FOREST FIRE 1997-1998 EPISODES ON MIXED DIPTEROCARP FOREST AT BUKIT BANGKIRAI, EAST KALIMANTAN [Pengaruh Musim Kering dan Kebakaran Hutan Episode 1997-1998 Terhadap Hutan Dipterocarp Campuran di Bukit Bangkirai, Kalimantan Timur] Simbolon, Herwint; Siregar, Mustaid; Wakiyama, Seiji; Sukigara, Naozumi; Abe, Yasuhisa; Shimizu, Hideyuki
BERITA BIOLOGI Vol 6, No 6 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Kawasan Kalimantan Timur mengalami musim kemarau panjang yang merangsang terjadinya kebakaran hutan yang luas pada 1982-1983 dan 1997-1998.Naskah ini mengemukakan hasil penelitian di Bukit Bangkirai, Kalimantan Tengah mengenai dampak dari kemarau panjang dan kebakaran hutan 1997-1998 terhadap diversitas tumbuhan pohon hutan dipterokarp campuran.Penelitian dilakukan dengan cara memperbandinkan hasil pencacahan tumbuhan pohon berlingkar batang setinggi dada lebih daripada 15cm yang terdapat dalam tiga petak penelitian, masing-masing: lha di hutan alam yang tidak terbakar (K-plot), 0,3 ha di hutan yang terbakar ringan (LD) dan lha di hutan yang terbakar berat (HD).Hasil penelitian menunjukkan bahwa musim kemarau panjang 1997-1998 menyebabkan 12,02% mortalitas individual pohon di hutan alam yang tidak terbakar, yang terlihat dari pohon mati berdiri tegak akibat kekeringan, atau kehilangan 21,67% dari total basal area. Secara keseluruhan terlihat bahwa kebakaran hutan menyebabkan kerusakan berat terhadap struktur dan komposisi hutan. Secara kumulatif, kemarau panjang dan kebakaran hutan menyebabkan mortalitas individual pohon berkisar 36-70% dan kehilangan total basal area antara 45-85% dan menyebabkan lantai hutan terbuka terhadap penyinaran matahari langsung karena menurunnya penutupan tajuk sebesar 23-79%.Kebakaran hutan juga berdampak terhadap penurunan biodiversitas tumbuhan pohon sebesar 23-79% pada tingkat jenis, 53-66% pada tingkat marga dan 18-21% pada tingkat suku.Tercatat perubahan komposisi jenis pada hutan yang terbakar; dominasi jenis Dipterocarpaceae dipetak hutan tak terbakar (K) menjadi dominasi pionir sekunder Macaranga gigantea-Vernonia arborea di petak terbakar berat (HD) dan dominasi Macaranga gigantea-Shorea smilhiana di petak terbakar ringan (LD).Beberapa jenis seperti Durio acutifolius dan Syzygium incarnaium mungkin dapat digolongkan sebagai jenis yang relative lebih tahan terhadap api.

AKTIVITAS ANTIBAKTERI AKTINOMISETES LAUT DARI PULAU ENGGANO [Antibacterial activity of marine actinomycetes from Enggano Island] Ratnakomala, Shanti; Apriliana, Pamella; Fahrurrozi, Fahrurrozi; Lisdiyanti, Puspita; Kusharyoto, Wien
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Marine actinomycetes were isolated from mangrove sediment from the coast in Enggano, Bengkulu Province, Indonesia using the medium NBRC No. 802 modified by the addition of 2% NaCl. A total of 29 isolates of actinomycetes were isolated from three mangrove sediment samples and evaluated their potential to produce bioactive metabolites. Screening of 29 isolates marine actinomycetes isolates were performed against three bacterial pathogens had been done. Bacteria test used was Escherichia coli NBRC 14 237, Staphylococcus aureus NBRC13 276, and Bacillus subtilis NBRC 3134. Screening result showed that seven isolates have inhibitory effects against bacteria test and 22 other isolates have no inhibition. Of the seven isolates, one isolate has inhibitory effect against the growth of Gram-negative bacteria Escherichia coli, while six other isolates inhibit Gram-positive bacteria Bacillus subtilis and Staphilococcus aureus. It was concluded that, of the 29 isolates conducted in the experiment, seven isolates produce antibacterial compounds on agar medium. Molecular identification of 23 isolates were identified based on Â the gene 16S RNA sequences showed that 22 isolates belong to the genus Streptomyces and one strain belongs to the genus Dermacoccus.

AKTIVITAS FOSFATASE TANAH DILINGKUNGAN BENTANG HUTAN ALAMI DAN NON-ALAMI Suliasih, Suliasih; Rahmansyah, Maman
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The spatial dispersal of acid and alkaline phosphates activity in forest soils appears to be controlled by position in the landscape and its soil microbial density. Soil bacteria expressively produced significant level of acid phosphatase in the investigation.The variety of acid phosphatase activity (8.25-37.55 />-nitrophenol.g"soil.h") noted higher and followed by alkaline (0.78-7.15 p-nitrophenol.g-soil.h" ), correspondingly. Acid and alkaline phosphates were exist in both of soil of natural as well as for non-natural forest soil landscape, with the value (po/o=0.6210) of correlation are 0.6889 and 0.6532, respectively. Phosphate Solubilizing Bacteria (PSB) average density in natural forest soil equivalent to 0.61xl0Ã colony forming unit (cfu) and total bacteria is 160xl0 cfu, while the PSB in non-natural forest soil is 7.75xl0 cfu and total bacteria is 48.25x10 cfu. Concerning to the forest environment as soil bacteria inhabitants of PSB, there were significantly difference of PSB population in natural and non-natural landscape forest. Forest environment is negatively affect the soil enzymes activities under Pinus maritima and Caliandra calothyrsus as the lowest activities, and to the highest ones under the Schima wallichii and Eucalyptus saligna vegetation.

KEANEKARAGAMAN TUMBUHAN VEGETASI HUTAN MANGROVE DI TUMBU-TUMBU, LAMPEAPI DAN WUNGKOLO, PULAU WAWONII, SULAWESI TENGGARA [Plant Diversity of Mangrove Forest Vegetation in Tumbu-Tumbu, Lampeapi and Wungkolo, Wawonii Island, South East Sulawesi] Suhardjono, Suhardjono
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fifty four species belong to 32 families and 46 genera were recorded in mangrove areas of Tumbu-tumbu, Lampeapi and Wungkolo(Wawonii Island, South East Sulawesi). Twenty four of them included in the IUCN (Anonim, 1997) list. The richest species diversity found in Lampeapi (48 species) followed by Tumbu-tumbu (39 spesies) and Wungkolo (29 species).The dominant species of those areas were Rhizophora apiculata, R. mucronata and Bruguiera gymnorrhiza.The total average density of three was 268â742 individu/ha, with basal area 16.14â28.99 m 2/ha, sapling plant was 66â317 indv./ha will basal area 0.38â0.92 m /ha and seedling plant was 88,13 â146,66 individu/ ha.

KARAKTERISASI ENZIM PROTEASE DARI BAKTERI Stenotrophomonas sp. ASAL GUNUNG BROMO, JAWA TIMUR [Characterization of Protease Enzymes of Stenotrophomonas sp. bacteria from Bromo Mountain, East Java] Soeka, Yati Sodaryati; Sulistiani, Sulistiani
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2940

Protease is an enzyme that can hydrolyze protein into simpler compounds, i.e peptides and amino acids. Microbial Proteases have the Â potency to be applied in industries such as detergents, skins, silver recovery, dairy, baking, beverages and pharmaceutical industries. These hydrolytic enzyme are efficiently involved in the food industry to increase the nutritional value, digestibility, palatability, flavour and reducing allergenic compounds as well as in the management of domestic and industrial wastes. The purpose of this study was to investigate the ability of Stenotrophomonas sp. isolated from Mount Bromo, East Java in producing protease. Protease activity of the bacterial isolate was qualitatively determined by formation of a clear zone surrounded their colonies on media containing skim milk (1%). We analyzed its Â proteolic activity against some effects of the incubation period, pH, temperatures and addition of monovalent and divalent metal ionsquantitatively using a spectrophotometer at ? 280 nm.The results showed that the optimum activity after incubation for two days was 315.88 U/ mL. The enzyme has continued to its activity at pH 8 (419.68 U/mL) and maintained its stability at 398.22 U/mL with activities decreased to 94.87%, while its activity at 60Â°C was 519.86 U/mL and could maintain its stability at 419.58 U/ mL, the activity decreased to 74.75%. The addition of Ca2+ could activated its enzyme activity at the amount of 424.33U/mL, while without addition of the ion its activity was 400.29 U/mL. The addition with ion MnÂ²+, K+, Na+ and Cu 2+ could act as inhibitors that might reduced the activity of the enzyme. Â

POLA PERSARANGAN CURIK BALI (Leucopsar rothscliildiStresemann, 1912) DAN KERAB ATNYA DI TAMAN NASIONAL BALI BARAT Noerdjito, Mas
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Bali myna (Leucopsar rothschildi Stresemann, 1912) was reported as an endemic bird from western part of Bali Island, and also as endangered species.The species lived in the lowland forest and used tree holes made by other animals as their nests.Besides L.rothschildi, there were two other species of birds found using the holes as their nest in the study site, namely Black-winged starling(Sturnus melanopterus (Daudin, 1800)) and Javan myna (Acridotheres fitscus (Wagler, 1827)).This paper deals with result of research on the competition of those three species of birds in obtaining tree hole for nesting, was carried out in West Bali National Park, Bali. Data demonstrated that there were no differences in the size of nest for the three species of birds, Javan myna lived in the habitat of coconut garden where Bali myna did not occupy it, and the habitat of Black-winged starling was overlapped with Bali myna, but Bali myna preferred living in denser vegetation. The reproduction of the three species of birds were dependent on the occurrence of other birds species belonging to Picidae and Capitonidae who were able to make holes on trees.

HIDROLISIS KITIN MELALUI FERMENTASI SEMI PADAT UNTUK PRODUKSI N-ASETILGLUKOSAMINA Saskiawan, Iwan; Handayani, Rini
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

N-acetyl-D-glucosamine (GlcNAc), the monomeric unit of polymer chitin has attracted much attention for their therapeutic activity in osteoarthritis.It is mainly produced by acid hydrolysis of chitin which affect the environmental problem because of its acidic wastes.Therefore, it is need to develop the new method for GlcNAc production. The aim of this experiment is to produce GlcNAc by mean of submerged fermentation of chitin. The preliminary study showed that fermentation of chitin by Aspergillus sp.501 produced higher GlcNAc than that of Saccharomyces sp. It was 1.39 ng/ml and 1.07 ng/ml. Then the Aspergillus sp. 501 was used in optimation of GlcNAc production. The effect of pH and nitrogen course such as bacto peptone, yeast extract, amonium sulfat and urea to GlcNAc production was examined. Then the product of GlcNAc was precipited by vacum evapotated and freeze dryed. The results showed that the highest of production GlcNAc of 2.228 ng/mL was obtained on pH 4 of medium solid state fermentation using urea as Nitrogen source at 10 days incubation.

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