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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
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MIKROBAENDOFITIK DARI TAMAN NASIONALBATANG GADIS SUMATERA UTARA: POTENSINYA DALAM MENGHASILKAN SENYAWA ANTIMIKROBATERHADAPMIKROBAPATOGEN Harmastini Sukiman; Sylvia Lekatompessy; Tiwit Widowati
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i6.858

Abstract

Batang Gadis National Park (TNBG) is one of the tropical forests in North Sumatra with megabiodiversity of flora and fauna including microorganism. Endophytic microbes conservation from varieties of forest plants in Batang Gadis National Park has been completed. Nineteen endophytes isolates from TNBG have been screened for their potential on producing bioactive compound against the pathogenic bacteria. Qualitative screening has been done using the growth agar media and identified the clear zone appeared surrounding the bacteria colony. The result indicated that isolate MSCI 87.4 showed high strengthening secresion (4.35) againts Xanthomonas campestris, whereas isolate MSCI 37.1 showed high secretion against Bacillus subtilis (2.69) and Escherichia coli (2.60). Isolate MSCI 37.4 showed potential on producing bioactive compound againts Staphylococcus aureus (4.41). Isolates MSCI 87.4, MSCI37.1, MSCI 37.4 and MSCI 58.1 even could produce bioactive compound against four pathogenic bacteria that are Xanthomonas campestris, Bacilus subtilis, Escherichia coli and Staphylococcus aureus, whereas isolate MSCI 15.5b potential on producing bioactive compound against two isolates namely Xanthomonas campestris and Bacillus subtilis. The endophytes bacteria mainly belong to the Gram negative group and four out of nineteen isolates tested belong to the Gram positive group. The cell mainly coccus and only one is bacilli without fiagella. The five most potential isolates has been maintained under freeze dried condition for futher conservation and study. Thin Layer Analysis using semipolar organic separation solution showed that most isolates identified were able to produce bioactive compound except two isolates i.e. MSCI 48.4a and MSCI 53.1; however further analysis is needed to confirm the product.
INDUKSI POLIPLOIDI DENGAN KOLKISIN PADA HIBRID F1 HASIL PERSILANGAN ANTAR SPECIES PADA TANAMAN PANILI ASAL CIAMIS Fitri Damayanti; Ika Mariska
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3455

Abstract

Steem root disease caused by Fusarium oxysporum Schl. f. sp. Vanillae (Tucker) Gordon represent one internal issues of vanilla development (Vanilla planifolia Andrews). To obtain resistance clone to the disease, it can exploit resource of wild vanilla (V. albida B. L Syn) through crosses. Hybrids which were interspecific crossed generally were sterile. To overcome sterility problem of the hybrids, chromosome doubling was made by colchicine application. Explant used globular structure of proembryo from F1 seed result from a cross between wild vanilla of Ciamis as female parents and cultivated vanilla clone of Ciamis as male parent. Concentration level colchicine used were 0.00%, 0.05%, 0.10%, 0.20% and 0.25% with period of treatment of 3 and 6 days. After colchicine treatment embryo cultures were subcultured into new mwdium that was basal media Murashige-Skoog enriched with 2.5 mg/l BAP. Result of the experiment showed that colchicine treatment,globular structure were F1 embryo tending to inhibit early regeneration. The cultures showed variabilities from treatment of colchicin 0.20% during of 6 day and 0.25% for 3 days. Phenotypic performance of the chromosome doubled hybrids showing great variation in color and vigor of the culture. Tetraploid plant(2n=4x=64) was obtained from the colchicine treatment of 0.25% for 6 days. Chromosome addtion was followed by improvement of cell dimensions and organ magnification.
STATUS DAN STRATEGI PEMULIAAN POHON EBONI (Diospyros celebica Bakh.) Budi Santoso
BERITA BIOLOGI Vol 6, No 2 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i2.1499

Abstract

Sejak tahun 1990 eboni telah dinyatakan sebagai jenis kayu yang terkena larangan tebang dan dilindungi.Sebagai tindak lanjut, peraturan itu diberlakukan pada Hak Pengusahaan Hutan agar tidak lagi menebang pohon secara serampangan serta mewajibkan penanaman pada areal-areal bekas tebangan.Semakin terbatasnya pohon eboni di hutan alam dikhawatirkan basis genetika dari jenis ini akan menyempit, sehingga menyulitkan pekerjaan pemuliaannya. Kegiatan pemuliaan tanaman eboni masih sangat terbatas.Balai Penelitian Kehutanan Ujung Pandang merintis dengan melakukan konservasi ex-situ_eboni dan uji keturunan.Fakultas Kehutanan Universitas Gadjah Mada melakukan penelitian uji keturunan dan uji provenansi.Di Sulawesi Tengah sebagai pusat eboni, kegiatan pemuliaan tanaman ini baru dilakukan pada tahap awal dengan kegiatan pemilihan pohon induk. Agar program pemuliaan pohon eboni dapat terarah dengan baik, sehingga tujuan dari program pemuliaan yang diinginkan dapat tercapai diperlukan strategi pemuliaan yang benar.Untuk mengelola program pemuliaan dengan kendala waktu dan sumber daya yang tersedia diperlukan suatu system yang efisien dan yang memperhatikan berbagai kepentingan.
KEBERADAAN DAN DISTRIBUSl JENIS-JENIS Trichoderma DI HUTAN KAWASAN TAMAN NASIONAL GUNUNG HALIMUN Nandang Suharna
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1184

Abstract

A study was conducted m determine the occurrence and distribution of Trichoderma species in forest in Gunung Halimun National Park. Location for data ollection were Cikaniki (1.100 asl), Citalahab (1.100 asl.)and Gunung Botol (1.500 asl, 1.600 asl,and 1.700 asl).Isolation of Trichoderma were from non vegetation soils, rhizosphere soils and stumps of three dominating forest trees i.e.Allingia excelsa,Caslanopsis javanicti and Schimu wallichii.The isolation method used were soil plate (non vegetation soil,Rhizosphere soil), dilution plate (non vegetation soil.Rhizosphere soil) and surface sterilization (stump).Trichoderma viride recorded as the commonest fungus of its occurrence and the most widespread in non vegetation soil in all location studied. Other Trichoderma species encountered were T. longibrachialuin, T. virens T. koningii.T. hamaium. T. pseudokoningii and T. polysporum. While in rhizosphere soils of the three dominating trees {Allingia excelsa. Schima wallichii. and Casianopsis javanica) located in Cikaniki, T. hamatum, was the commonest fungus of its occurrence. Another common was T. koningii. Other species were rarely encountered were T. longibrachialum, T. virens, T. pseudokoningii and T. polysporum, and T. fertile. In stumps of four trees in Cikaniki four species of Trichoderma were recorded of their occurrences. They were T. harzianum (Memecylon excelsum). T. koningii (S. wallichii), T. viride (A. excelsa, S. wallichii and C. javanica) and T. virens (A. excelsa and M. excelsum). It i> concluded that in forest of Gunung Halimun National Park, habitat influenced on the occurrence and distribution of Trichoderma species, but not for altitude. Trichoderma was high in diversity, dominant in occurrence and widespread in distribution.
KEANEKARAGAMAN TUMBUHAN PAKU TERESTRIAL DI HUTAN KOTA DKI JAKARTA Dwi Andayaningsih; Tatik Chikmawati; Sulistijorini Sulistijorini
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.638

Abstract

Urban forest is one of the green open area consisted of natural forest-like vegetation. It has suitable microclimate for understory habitat, including terrestrial ferns. The diversity of fern in three urban forest in DKI Jakarta is reported, i.e the urban forest at The University of Indonesia (UI) Campus, The Cibubur Arboretum and PT JIEP (Jakarta Industrial Estate Pulogadung). Vegetation analysis was conducted by purposive sampling method with a transect line of 200 m in each location with 15 plots of (3x3) m2 each. Fern determination was done based on herbarium specimens and literatures. Fern diversity was analyzed based on Shannon diversity index, and equality index. Canonical correspondence analysis (CCA) was performed to determine the correlation between fern diversity and abiotic factors. There were 18 fern species classified into 11 genera and eight families, at the UI urban forest and Cibubur Arboretum, but there was no fern in PT JIEP. Fern with the highest importance value index was Sphaerostephanos sp. (161.33%) in The UI urban forest, and Lygodium microphyllum (Cav.) R.Br. (71.63%) in Cibubur Arboretum. The CCA result showed that the fern diversity correlated to four abiotic factors i.e temperature,humidity, light intensity and soil pH.
ODONTOCHILUS UNIFLORUS (BLUME) H.Ǣ. PEDERSEN & ORMEROD: A NEW ADDITION OF THE JEWEL ORCHIDS FOR FLORA OF JAVA Lina Susanti Juswara
BERITA BIOLOGI Vol 19, No 1 (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i1.3817

Abstract

Odontochilus uniflorus (Blume) H.Ǣ. Pedersen &Ormerod, is a new addition to Flora of Java based on three specimen collected from Mt. Halimun-Salak National Parks. This species was previously known only from Thailand, Malaysia, and Sumatra. Hence, this new finding expands the range of distribution of the species of O. uniflorus as well as adding information for orchids of Java. Identification key of the species of Odontochilus in Java is also presented in this paper.   
EKSPLORASI DAN IDENTIFIKASI MIKORIZA INDIGEN ASAL TANAH BEKAS TAMBANG BATUBARA Margarettha Margarettha
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1923

Abstract

Attempts to recovery the degraded soils are applied with a friendly environment agricultural concept, such as bioremediation by potential indigenous microorganism like mycorrhyza. The objective of this research is to explore and identify indigenous mycorrhyzas of ex-mining coal soil. Soil samples were obtained as random sampling from top soil and stock pile (depth 0- 30 cm).Furthermore, identifying and trapping of mycorrhyza were also studied. The results obtained 13 types of indigenous mycorrhyzas i.e. Glomus sp. with 9 spore types, Acaulospora sp. with 3 types of and 1 type from Enthrospora sp.
AKTIFITAS BIOREPRODUKSI GENERATIF DAN KEMUNGKINAN OTOFERTILISASI PADA BUNGA BEBERAPA JENIS ZINGIBERACEAE MINOR LIAR Erlin Rachman
BERITA BIOLOGI Vol 4, No 2&3 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i2&3.1283

Abstract

ApreBminary study on flowers of some wild-minor Zingiberaceae species was conducted to know their generative reproduction andpossiblefertilisation mechanism. Nine species from five wild-minor Zingiberaceae genera grown in Bengkulu Forestry Grand Park :Achasma macrocheilos Griff., A.triorgyale Bak.,Amomum aculeatum Roxb.,A.maxima Roxb.,A.testaceum BJdl, Homstedtia minor VaL, H. scyphus Ret.Nicolaia speciosa Bl, and Zingiber aromaticum VaL, were in situ observed. As much as 90% of the species were found in flowering stage in the study periods, between September and December 1995, 75% of the plants set fruit and seed while 25% set seeded fruit according to native people from neighbour village. All of the flowers showed that many spikelet might be anthesi edper spike per day and were terminally heated on non leafy "stem".Some stigmas observed were occupied by some pollens though pollinator was absent. In addition, stigma - anther distances were very near (0-3 mm).We found that generative bioreproduction was active and productive and autofertili^ation mechanism might be considered to be occurred on the species.
ANALISIS SENYAWA BIO AKTIF DARI EKSTRAK BUI PICUNG (Pangium edule Reinw.) SEGAR Wibowo Mangunwardoyo; Lily Ismaini; Endang Sri Heruwati
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.781

Abstract

Pangi (Pangium edule Reinw.) seed has long been used traditionally as a preservative agent for fish, especially in remote areas.A study has been conducted on analysis of bioactive compounds of pangi fresh seeds extracts and their fractions.In this study,maceration of fresh seed using water and 50% ethanol was carried out followed by thin layer chromatography (TLC) analysis to see weather the extracts contained tannin. Both extract then separated into their respective fractions using column chromatography.Fractions which had been tested to have highest antibacterial activity were then analysed using gas chromatography mass spectrometry (GC-MS) to assess the active compounds which believe to be a preservative agent.Identification of water and 50% ethanol extract of Pangium edule Reinw.fresh seeds with TLC resulted that tannin were found in those extracts. Furthermore, GC-MS analysis showed that fractions which had been previously tested to have high antibacterial activity contained 9-octadecanoic acid whith similarity index of 89%, and 1,2-benzenedicarboxylic acid whith similarity index of 94-85%.
KARAKTER RESPIRASI DAN MINERALISASI KARBON ORGANIK PADA SAMPEL TANAH DIKOLEKSI DARI PULAU BANGKA Maman Rahmansyah; Suliasih Suliasih
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i1.2855

Abstract

The study was designed to explore soil biomass content and soil enzymatic activities that involved in carbon organic soil mineralization. Samples of soil were collected from two locations in Bangka Island. Bulk samples of top soil (TP) and excavated (TG)  were collected from garden soil in Pangkalpinang. Other soils were gathered from forest floor (TU), beneath pelawan (Tristaniopsis merguensis Griff.) trees which is endemic plant to North Bangka forest. Soil biological character was evaluated by measuring soil microbial population, respiration rate, and cellulase and amylase activities. Experiments were carried out in Research Center for Biology, Indonesian Institute of Sciences, on dried soil treatment (Control), soil moistened with 5 mL H2O in 100g soil (Gluc.0), and soil moistened with 5 mL 1% glucose solution (Gluc.1). Bacterial population obtained was higher than fungal population in all soil samples. Soil respiration activity in TU after treatment was 4.51±0.49; 5.26±0,79; and 8.28±1.41 ppm CO2/100g-soil/5-minutes, respectively. Meanwhile , respiration in TP  were 3.65 ± 2.12; 3.57 ± 1.18; and 7.94 ± 1.05 ppm CO2/100g-soil/5-minutes; and in the TG are 2.61 ± 0.70; 3.34 ± 0.94; and 5.46 ± 2.30 ppm CO2/100gsoil/5-minutes. Cellulase activity of all samples were not significantly different. Forest soil cellulase activity was significantly different compared to garden soil. Glucose induction was positively increased amylase activity compared to cellulase. Soil enzyme, soil respiration activities and microbial population of forest soil could be used as a reference to recover degraded land in Bangka Island.

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