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Articles 2,125 Documents
INFEKSI Salmonella enteritidis PADA TELUR AYAM DAN MANUSIA SERTA RESISTENSINYA TERHADAP ANTIMIKROBA Anni Kusumaningsih; M Sudarwanto
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1944

Abstract

Salmonella enteritidis is one of pathogenic bacteria in chicken and human with re-emerging foodbome pathogen. Uncontrol use of antimicrobial drugs for the prevention and treatment of infectious diseases in chicken and human may lead to the development of antimicrobial resistance. Sampels of chicken eggs were collected from markets, layer farms, and grant parent stock farms. Sampels from human were anal swabs collected from hospitals and a laboratory of microbiology. Identification of bacteria was conducted by isolation and biotyping using selected media for Salmonella, while serotyping S. enteritidis was conducted with spesific 0 somatic (1, 9, and 12) and H flageIJa (m) antigen.Antimicrobial resistance tests were conducted by the standard diffusion method using antimicrobial disks.The results of isolation and identification of Salmonella show that 9 out of 122 (7.4%) sampels of layer eggs and 7 out of 23 (30.4%) sampels of embrioneted eggs were positive with Salmonella spp. The results of serotyping against S. enteritidis show that 7 out of 9 (77.0%) from layer eggs and 7 out of 7 (100.0%) from embrioneted eggs were positive with S. enteritidis.From the IS anal swabs of human were obtained 14 (93,3%) contain S. enteritidis.The antimicrobial resistance profiles of S. enteritidis isolated from layer eggs show high resistances to streptomycin (42.9%), neomycin (85.7%), doxicycline (64.3%), and ciprofloxacin (57,1%), whereas those S. enteritidis isolated from human show high resistances to streptomycin (50.0%), neomicyn (85.7%), tetracycline (42.9%), and doxicycline (42.9%).Multiple resistance profiles of S. enteritidis isolated from eggs were mostly to 2-3 antibiotics and those of S. enteritidis isolated from human were mostly to more than 5 antibiotics.
FITOSOSIOLOGI HUTAN Dl KAWASAN PENYANGGA DANAU SEMAYANG DAN MELINTANG, KALIMANTAN TIMUR Mustaid Siregar
BERITA BIOLOGI Vol 4, No 1 (1997)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i1.1292

Abstract

This study was aimed at describing the forest types and their degradation at buffer zone of Semayang and Melintang lakes,East Kalimantan.The study was carried out in October 1994 by plot method.Within twenty five main plots, 108 tree species belongs to 75 genera and 38 families were recorded.Ouster analysis shows, the plots have high variation in species.By 50 % similarity index,there could be identified five communities (units)in peat swamp forest,eleven units in riparian forest and two units in kerangas forest.Structure, floristic composition and their degradation of communities at each forests are discussed.
INDUKSI KALUS DAN ORGANOGENESIS KULTUR IN VITRO Dendrobium lineale Rolfe Djadja Siti Hazar Hoesen; Witjaksono Witjaksono; LA Sukamto
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.790

Abstract

Callus induction and organogenesis were evaluated in the protocorm like bodies (PLB) culture of Dendrobium lineale Rolfe (Orchidaceae). The globular PLB were cultured in Murashige and Skoog (MS) medium with half strength macronutrients QA MS), supplemented with thidiazuron (TDZ 0,1, 0,5 and 1) mg/1 and 2,4 Dichlorophenoxy acetic acid (2,4-D 0.1, 0.5, 1 and 5) mg/1 as treatments. The media containing 1 mg/1 TDZ and (1 and 5) mg/1 2,4-D was the best treatment for callus initiation (100%). The largest diameter of callus was obtained from 1 mg/1 TDZ (average 3.5 cm). Shoot buds regeneration achieved on 1 mg/1 TDZ (average 41.66 %). However roots regeneration was very low (average 6.66 %) obtained from 1 mg/1 2,4-D. The number of adventitious buds produced from the regenerated shoots on media without plant growth regulator
ANALISIS DELIMITASI JENIS PADA Monascus Spp. MENGGUNAKAN SIDIK JARI DNA ARBITRARY PRIMER PCR Nandang Suharna; Heddy Julistiono
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2928

Abstract

A species delimitation analysis within Monascus spp. using Arbitrary Primer Polymerase Chain Reaction (AP PCR) DNA fingerprint was carried out. This is one of the methods used for identification and discrimination of bacterial strains within the same species. Its advantages including using single primer, independent of DNA quality, and observing amplicon shared by only some strain. This study analyzed Monascus sp. MM isolate which was originated from a source contaning high level of ethanol and two M. purpureus isolates which isolated from angkak. However, based on ITS region, 99% homology showed the unclear species delimitation. Therefore, this analysis was aimed at clarifying on the identities of Monascus species tested. The result showed DNA polymorphism among three isolates of Monascus that showed species delimitation. This study showed that species delimitation within Monascus isolates used in this analysis could be supported by AP PCR DNA fingerprinting. Therefor we suggested to use this technique or method for phylogenetic study to clarify taxonomic position of Monascus strains. 
FERMENTASI BAHAN ONCOM BANDUNG DUDI D. SASTRAATMADJA; SUSONO SAONO
BERITA BIOLOGI Vol 2, No 9&10 (1984)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v2i9&10.1421

Abstract

Oncom teimasuk makanan yang harganya murah tetapi mengandung protein yang relatif tinggi. Di Jawa Barat makanan ini telah lama dikenal serta banyak dimakan. Pembuatannya dilakukan secaia tiadisional dengan menggunakan bahandasar bungkil kacang tanah dengan campuran onggok/ampas tapioka dan ampas tahu, atau bahan dasar ampas tahu dengan campuran onggok. Sampai saat ini belum banyak usaha yang dilakukan untuk mengetahui seluk beluk ilmiah produk ini, apalagi usaha terencana untuk meningkatkan mutu seita menyebarluaskan pemanfaatannya. Hal ini dapat disimpulkan dari terbatasnya pustaka yang ada (Dwidjoseputro et al. 1970, Van Veen et al. 1968, Steinkraus & Van Veen 1971, Muhilal et al. 1970; Slamet & Tarwotjo 1971; Saono et al. 1974).
PERTUMBUHAN BEBERAPA ISOLAT MIKROBA DARI BERBAGAI LIMBAH INDUSTRI PADA BENZAMIDA Nunik Sulistinah; Bambang Sunarko
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1104

Abstract

Twenty five microbes could be isolated from industrial effluents.Seven isolates of those examined microbes were able to grow on benzamide as sources of carbon,energy,and nitrogen..The highest growth on benzamide was shown by bacterial isolate D1.Besides on benzamide, isolate D1 could grow on acetamide,acrylamide, benzamide,nicotinamide and propionamide, respectively.. On carboxylic acids, however isolate D1 could grow only on acetic acid, propionic acid, and benzoic acid as carbon and energy sources.When isolate D1 grew on 40 mM benzamide, the doubling time(\j was 6 h 40 minutes, the specific growth rate (\J) was 0,046 h'\ the attained maximum cell biomass was 4.96 g cell dry weigtAiter medium, and the yield coefficient (Y) was 124 g cell dry weight/mole benzamide.
ADAPTASI OSMOTIK TUMBUHAN MANGROVE Avicennia marina (Forsskål) * Vierh. DAN KEDELAI (Glycine max (L.) Merr.) TERHADAP STRES SALINE Beth Paul Naiola
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.480

Abstract

The extent of salinity effect land due to bad agricultural practices and global climate change that could affect the productivity of cultivated plants in agricultural and coastal areas as a result of rising sea levels, encourage the study of cultivated plants resistance to salinity stress. This research is aimed to observe the mangrove Avicennia marina (Forsskål) Vierh., a halophyt, grew up on the beach as a plant model that is able to adapt to saline environment by pumping out the NaCl (sodium pump) and deposited on the leaf surface. On the application of sea water with a multilevel concentrations (0, 25, 50 and 75%) A. marina is able to adjust by changing the osmotic potential in line with the level of concentration of sea water, as an indication of the course of osmotic regulation. Meanwhile, soybean var. Anjasmoro only able to adapt to sea water at a concentration of 25%, equivalent to 150 mM NaCl. This result may be the starting point by breeders and biotecknolo gists in utilizing the potential genes drives the salinity resistance characters in Indonesian mangrove species (especially Avicennia marina) to contrive salinity resistance cultivars of cultivated food plants with better yield.
PERSPEKTIF GENDER SUKU OSING DI BANYUWANGI DALAM PENILAIAN KEMANFAATAN TANAMAN Budi Prasetyo; Tatik Chikmawati; Eko Baroto Walujo; Ervizal A.M Zuhud
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.3689

Abstract

The different roles and responsibilities of male and female of the Osing tribe influence the knowledge of the use of plants. The aims of research is to analyze the values of the benefits of plants based on gender perception. The research used a qualitative and quantitative methods. Data collection for the value of plant species utilization were carried out  using Pebble Distribution Method (PDM) and analysis used Index of Cultural Significance (ICS). The Osing community recognised 86 plant species that were grouped in to eight beneficial groups. The male in three villages have a better understanding of ethnobotanical knowledge and practice than female on crops as staple foods, medicines, fruits, and firewood, while understanding of ornamental plants are relatively the same for both male and female. Female have a broader and more detailed understanding on vegetables, plant species used as building construction, and traditional rituals. The high PDM value in eight beneficial groups was supported by the high value of each ICS.  
ANALISA KANDUNGAN LOVASTATIN, PIGMEN DAN CITRININ PADA FERMENTASI BERAS IR-42 DENGAN MUTAN Monascus purpureus N Nurhidayat
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1888

Abstract

The need of medicine product are increasing now.The fermentation of rice by using Monascus purpureus produce lovastatin, pigments and citrinin as secondary metabolites. The aim of this study was to know the concentration of lovastatin, red and yellow pigments and citrinin in rice which fermented by Monascus purpureus that had been mutated by using ultraviolet irradiation.Three strains were used in this research, such as AsK, yellow Jmba and red Jmba.The result were analyzed by using HPLC and Spectrofotometer.The result showed that the yellow Jmba strain which treated by UV mutagenesis for 3 minutes contain higher lovastatin (1.798%) and pigment and lower citrinin (0.019%) compare to without mutagenesis.On the other hand ASK strain produced higher lovastatin (1.903%) but higher citrinin (1.270%).This research is expected to provide scientific information to the public.That M. purpureus contained lovastatin that is beneficial,red and yellow pigments for natural food colorant.
GAMBARAN MORFOLOGI BUAH/BIJI PERUD PUSPA (Balanophora fungosa J.R. & G. Forst. ssp. indica (Arn.) B. Hansen var. globosa (Jungh.) B. Hansen) Sunaryo Sunaryo
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1253

Abstract

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