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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 138   139   140   141   142 
EVALUASI KERAGAMAN FENOTIPE GALUR MUTAN PISANG BARANGAN {Mmaparadisiaca kuitivar Berangan) PADA GENERASI M, V4 Azri Kusuma Dewi; lta Dwimahyani
BERITA BIOLOGI Vol 7, No 6 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i6.864

Abstract

Phenotypic variance derived from banana [Musa paradisiaca) mutant lines of cv. Barangan were carried out in nursery level which were conducted at CRDIRT-Green House, Pasar Jumat, Jakarta. Several phenotypic performances of mutant lines such as leaf and pseudostem, petiole and leaf length, plant height, leaf width were observed. Number of 150 plants, were then divided into 11 groups based on performance of leaf, pseudostem, and petiole length. Results indicated that 26% was showed for normal performance,while 6,7% was showed for abnormal lamina with normal petiole, 11,3% was showed for abnormal lamina and petiole, 28,7% was given for stunted plant, 3,3% with chocking lamina, 1,3% was showed for abnormal petiole, 2,0% with light green leaf and abnormal petiole, 6,0% was given for reddish pseudostem, 0,7% with cigar leaf, 1,3% was showed for discoloration leaf and 2,7% was showed for elongation leaf and abnormal petiole. From the result of this experiment can be concluded that gamma irradiation with dose 15 Gy produced high phenotypic variance of cv. Barangan.
KUALITAS SEMEN BEKU DOMBA GARUT (Ovis aries) PENAMBAHAN SUKROSA DALAM PENGENCER SEMEN KUNING Herdis suharman
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i1.2294

Abstract

The objective of this research was to examine the effect of sucrose in improving the quality of the plasma membrane intact and sperm motility of frozen semen of Garut ram. Semen was collected using artificial vagina weekly from six mature garut rams.   Immediately after initial evaluation, fresh semen was divided into four parts and diluted with Tr s extender without sucrose (T0), Tris extender + sucrose 0.2g/100 ml (T1), Tris extender + sucrose 0.4g/100 ml (T2) and Tris extender + sucrose 0.6g/100 ml (T3), respectively.  Results of this research showed that the percentage of sperm motility after thawing in T2 (49.00 ± 5.48%)  was significantly (P<0.05) higher than T0 (42.00 ± 2.74%) but was not significantly difference (P>0.05) than T1 (46.00 ± 4.18%)and T3 (48.00 ± 4.47%).   Evaluation of plasma membrane intact showed that T1 (62.33 ± 6.51%) was s gnificantly different (P <0.05) w h T0 (49.40 ± 2.19%) but was not significantly different (P> 0.05) than T2 (58.50 ± 4.97%) and T3 (56.40 ± 5.90%).  In conclusion, he addition of sucrose in semen extender  improved the quality of frozen semen of Garut ram.  Concentration of 0.2g / 100 ml is the op ma dose to improve the quality of the plasma membrane intact and motility of spermatozoa during the freezing process.
SILVIKULTUR CENDANA: MENCARILUASAN BUDIDAYA YANG LAYAK EKOLOGIS DAN EKONOMIS Endang Ahmad Husaeni; Sudaryanto Sudaryanto
BERITA BIOLOGI Vol 5, No 5 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i5.1461

Abstract

Kayu cendana memainkan peranan sangat penting dalam perekonomian Propinsi Nusa Tenggara Timur (NTT), melalui sumbangannya sebesar 40% per tahun terhadap PAD.Sumbangan sebesar itu berasal dari pengusahaan hutan alam yang potensinya terus menurun dari tahun ke tahun. Untuk mempertahankan dan sekaligus meningkatkan peranan cendana terhadap perekonomian Propinsi NTT, selain tetap mengusahakan hutan alam yang ada, perlu dibangun kelas perusahaan cendana seluas 10.000 ha,dengan menerapkan sistem silvikultur THPB dan silvikultur intensif.Dengan daur (umur tebang) 50 tahun kelas perusahaan tersebut mampu memproduksi kebutuhan dalam negeri dan untuk ekspor.Pembangunan kelas perusahaan cendana ini dilaksanakan bersama masyarakat di Pulau Timor, Sumba dan Flores, pada ketinggian tempat sekitar 700 m dpi.Adanya kebun benih sangat diperlukan untuk memasok kebutuhan benih setiap tahunnya.
PENGARUH PEMBERIAN EKSTRAK Saccharomyces cerevisiae Hansen TERHADAP KANDUNGAN GOSIPOL PADA KULTUR KALUS Gossypium hirsutum L. Rama Riana Sitinjak; Arbayah H Siregar; Rizkita RE
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1133

Abstract

This study concentrated on the effect of Saccharomyces cerevisiae Hansen extract on gosipol content of Gossypium hirsutum L callus cultures. There were many ways commonly used to obtain high secondary metabolites product by using tissue culture,i.e. elicitation.The objective of this study was to increase the production ofgossypol on G. hirsutum callus cultures using elicitor derived from S. cerevisiae.The callus grew optimally and produced gossypol on Linsmaierand Skoog (LS) medium with the addition of 10 M NAA and 10 M 2,4-D. Callus derived from cotyledon was subcultured five times.Callus was then elicited with elicitor derived from autoclaved S. cerevisiae and concentration of elicitor tested were 0.5, 1.0 and 2.5% (b/v). The harvesting times were 0, 2, 4, dan 6 days after elicitation. The gossypol was analyzed qualitatively and quantitatively by using High Performance Liquid Chromatography (HPLC).Gossypol content was influenced significantly by concentration of elicitor and harvesting time.The maximum ofgossypol content was obtained on the 4 day after elicitation i.e. 469.233 ± 2.332 pg/g dry weight in the elicited callus of G. hirsutum.
STRUKTUR DAN KOMPOSISI VEGETASI SEKITAR SARANG PENYU HIJAU (Chelonia mydas Linnaeus) PANTAI PANGUMBAHAN, SUKABUMI SELATAN, JAWA BARAT Roemantyo Roemantyo; Adriani Sri Nastiti; Ngurah N Wiadnyana
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i3.508

Abstract

Pangumbahan coastal area is one of many important nesting sites of the green turtle (Chelonia mydas Linnaeus) in Indonesia. This area is mainly comprises of disturb coastal old secondary forest. Some sites along the seashore were converted into fishpond, dry land agricultural and other was destroyed for other uses or as an open unproductive areas. However this area has a still plays an important role especially for the conservation of green turtle habitat and also for the ecosystem stabilization of the coastal area in general. Floristic research was conducted on September 2009 to investigate the existing vegetation structure and composition along the seashore using transects method.The “point center quarters method” was used to calculate the importance value of the vegetation on every turtle nesting sites. The important natural species vegetation communities in the area were comprises of herbs, shrubs and trees species. Ipoemoea pes-caprae (L.) R. Br and Spinifex littoreus (N. L. Burman) Merrill as a first layer coastal line plant community that play as an important herbs species which creeping grown on the white sands coastal surface. The second layer comprises herbs, small trees and shrubs such as Pandanus tectorius Parkinson ex Zucc, Crinum asiaticum L. and Callotropis gigantea R.Br. The second layer species plays as a vegetation community which covering and protecting the green turtle nest site from the direct sunshine, running of big wave and heavy rain water. Terminalia catappa L., Calophyllum inophyllum L., Barringtonia asiatica (L.) Kurz. and Hibiscus tiliaceus L. as a big crown tree community grown covering on the most behind as a back layer of coastal line which play as shading trees of the second layer species community, especially to stabilized humidity and temperature of the sand and the environment. The vegetation structure and composition of the old secondary coastal forest of Pangumbahan was described to understand the detail role and function of the vegetation species in this area.
REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.) Yohana C Sulistyaningsih; Alex Hartana; Utut Widyastuti; Hamim Hamim; Suharsono Suharsono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1940

Abstract

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.
KANDUNGAN SENYAWA ALKALOIDA, TANIN SERTA NILAI NUTRISI BEBERAPA JENIS HIJAUAN YANG DIBERIKAN PADATERNAK Dl PULAU TIMOR (The Contents of Alkaloid, Tannin and its Nutritional- Values from Several Browse Fed to Livestock in Timor Island) Yuliasri Jamal; Gono Semiadi
BERITA BIOLOGI Vol 4, No 1 (1997)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i1.1288

Abstract

A study was conducted in determining the contents of alkaloid, tannin and nutritional Values from six browses fed to fattening cattle in Timor island. The samples Were gmelina (Gmelina arborea Roxb.),pates/lamtoro (Leucaena leucocephala [Lamk.J De Wit),daun kupu-kupu (Bauhinia malabarica Roxb.), gala-galaAuri (Sesbania grandiflora [Li Pers.l gamal (Gliriddia sep/um [lick] Steud.).and kabesak (Acacialeucoohloea Willd.).Samples were collected during wet and dry seasons.Results showed that total number of alkaloid compounds varied from 14 to 30.There was an increase in concentration for alkaloid and tannin from Wet to dry season, however the concentrations were low (< 1%).The increase in the concentrations between seasons Were ranged from 20% to 32096.Nutritional values of the browse during wet season were considered high, however there is a need in evaluating the nutritional values of the browses during dry season as well.
AKTIVITAS ENZIM PROTEASE DARI Mucorjavanicus YANG DITUMBUHKAN PADA MEDIA TEPUNG SINGKONG {Mannihot utilissima) Abdul Choliq
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.786

Abstract

The experiment was conducted to study the activity of protease from Mucor javanicus in cassava flour media. The influence of peptone and incubation time to the production of the enzyme,and the influence of pH and temperature to the activity of protease were studied. The production of protease was conducted in the flask (100 ml) which contain 17.5 g cassava flour and SO ml aqueous media.The species of M.javanicus observed consist of 10 strains. The strain of M.javanicus which has the highest production of protease among the tenth was incubated in cassava flour media with 3 variations of incubation time (4, 7 and 10 days) and 5 variations of peptone concentration (1-5%). The pH and temperature used for protease characterization were pH 4.0-7.0 and 30-70°C, respectively. The results show that the highest protease production with the optimal incubation period 7 days (0.51 U/ml)was M. javanicus M3. The optimal concentration of peptone for protease production was 2 %. The optimal protease activity was reached in pH 5 and temperature 50°C with the value of 4.53 U/ml.
RESPON BEBERAPA KULTIVAR PADI SAWAH PADA PENGAIRAN SISTEM GENANGAN DALAM PARIT Syamsuddin Syamsuddin; D. Indradewa
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i1.2860

Abstract

Efficiency of water use in paddy rice irrigation system is necessary to increase cropping intensity. Development of rice cultivars that are responsive to adequate soil moisture with high yield and efficient use of water is required for tropical paddy fields. The research was conducted in Sleman district of Yogyakarta province, from April to August 2011. The study was conducted on some selected rice cultivars to be cultivated on soil saturated culture (SSC) system to asses the response of cultivars that can produce high grain yield. Experiment was using randomized block design (RBD) and environment factors as replications. Control treatment was rice field. Rice cultivars used are IR.64, Ciherang, Inpari-1, Inpari-2, Inpari-3, Inpari-4, Inpari-10, Inpari-6 Jete, Cigeulis, Ciliwung, Luk Ulo, Way Apo Buru, Widas, Bondoyudo, Silugonggo, Tukad Petanu, Batang Piaman, Cibogo, Dodokan, Sarinah, OM.5637, WS.63-22-1-1-3-3, Inpari-8, Situ, Bagendit, and Cimelati. The results showed that the cultivars Cimelati and Sarinah was a cultivar response to the SSC system compared to other cultivars. The Cimelati cultivar grain yield potential was 25.02 g/hill and Sarinah was 21.45 g/hill. The yield using SSC system was higher compared to rice fields system.
SERANGGA PERUSAK KECIPIR (PSOPHOCARPUS TETRAGONOLOBUS L.) ANITA HANNA; S. ADISOEMARTO
BERITA BIOLOGI Vol 2, No 9&10 (1984)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v2i9&10.1417

Abstract

Kecipir kini merupakan tanaman protein yang mulai digalakkan pengembangannya karena kandungan protein yang hampir sama dengan kedelai. Biji kering mengandung 2.9,8 - 37,4% protein, daun 5,7 - 15%, bunga sekitar 5,6% dan polong muda 1,9 - 2,9% (NAS 1975, Citroreksoko 1977). Banyak bagian tanaman kecipir, seperti daun muda, polong dan biji, serta pada beberapa vaiietas juga juga umbinya, dapat dimakan (Burkill 1936).Penggalakan pengembangan budidaya kecipii ini memerlukan perhatian masalah hamanya. Penelitian mengenai hama kecipir telah dilakukan di berbagai negara, di antaranya ialah Papua Niugini, Malaysia dan Filipina (Price 1978). Di Indonesia sendiri penelaahan terhadap hama kecipir masih agak diabaikan. Dari pengumpulan data yang telah dilakukan temyata bahwa kecipir di Indonesia mempunyai pula musuh alami yang berpotensi dan kalau dibiarkan dapat menggagalkan usaha penanamannya (Adisoemarto dkk. 1978). Mengingat permasalahan hama yang timbul perlu dilakukan penelaahan secara kualitatif serangga hama yang berhubungan dengan kecipir.

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