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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 168   169   170   171   172 
RESPON GENJER {Limnocharis flava (L.) Buchenau.} TERHADAP PEMUPUKAN DAN POTENSI GIZINYA UNTUK DIVERSIFIKASI KONSUMSI SAYURAN Titi Juhaeti
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.523

Abstract

The research was carried out to study the effect of fertilizer on the growth (plant height, number of leaf, length and width of leaf, fresh weight) and flower production of velvet leaf – genjer {Limnocharis flava (L.) Buchenau} and to analyze nutrient potential i.e. proximate and amino acids in leaves and flowers (including stalk). Seedlings of velvet leaf with 6 leaves were planted and fertilizer treatments were applied in one week after planting. Planting media were mixture of soil:manure:compost (2:1:1). The fertilizer treatments included control, NPK2, NPK4, MU2, MU4, POH2 and POH4. The plants were cultivated until 5 Weeks After Fertilizing (WAF). The results showed that velvet leaf can be successfully cultivated in plastic pot on planting media added with fertilizer. The highest plant growth and flower production were in the MU4 treatment (NPK Mutiara 27-5-5, 4g/plastic pot). Application of starTmik Biofertilizer 4cc/50cc water/plastic pot showed as good performance as the MU4 treatment, however, the dosage and application frequency need to be increased. The proximate analysis on leaves and flowers showed considerably high percentage of crude fibre (1.56% and 1.42%), protein (2.04% and 1.98%) and carbohydrate (3.16% dan 2.98%). Amino acid analysis revealed 9 numbers essensial amino acids and 8 number non essensial amino acids.
PRIMER-PRIMER BARU UNTUK MENGAMPLIFIKASI GEN PENGKODE PROTEIN AMPLOP VIRUS DENGUE STRAIN CH53489 Ira Djajanegara
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1969

Abstract

Restriction site of BamHI and Sail must be added in order to express the gene encoding envelope protein of dengue virus strain CH53489 (gene E) into expression vector pMAL-p2x. This approach required the PCR technique for amplification as well as restriction sites addition. However, PCR amplification is prone to error due to the process of misincorporation eventhough using Platinum taq polymerase. Therefore, it is important to be concern that there will be no alteration of the gene especially for biopharmaceutical purposes such as recombinant vaccine. This experiment was aimed to design several primers of DenV-M F, D3-1715s, D3-2117s, D3-1911c and DenV-M R for full length sequencing of the amplified products. Primers were designed in silico using Oligo Explorer and tried in vitro to check the ability of the primers to produce fragments. The sequencing results showed that the amplified product suffered from misincorporation during amplification (98.9% homology). However, the 3-D protein structure prediction did not show any major changes in the protein structure. Further analysis of the expressed protein is required to be used for biopharmaceutical purposes.
PENGARUH PENGERINGAN TERHADAP KANDUNGAN LEMAK CABE BESAR (CAPSICUM ANNUUM L.) YULIASRI JAMAL; G. PANGGABEAN
BERITA BIOLOGI Vol 3, No 8 (1988)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i8.1303

Abstract

YULIASRI JAMAL&G. PANGGABEAN. 1988 The effect of drying on the fat content of th chillies (Capsicum annuum L.). Berita Biologi 3(8) 374 - 376. The treating of chillies on various temperatures were studied in order to know their effect on the fat content.The chillies were dried in the refrigerator (12°C),in the room at ambient temperature (25°C ±2°C), in the sun shine (35°C ± 2°C) in the oven at various temperatures such as 40°C 50°C, 60°C, 70°C, 80°C, 90°C and 100°C.During the investigation were used cabe keriting and cabe besar biasa & it were obtained from the local market.The results of the observation that the highest fat content were found on the cabe keriting a 70 C drying as well as room temperature.The fat content of terated chillies, however, in the oven at 50°C, 60°C were similar 14%.Meanwhile,the dried chillies as the result on the sun shine was 3,5% fat content. The fat content of cabe besar biasa was significantly high at 70°C. The fat content of the chillies decreased rapidly on the treating in the sun shine.The chillies both cabe keriting and cabe besar biasa which were dried more than 60°C & in the sun shine, the pulp turned to brownish red, whereas the dried chillies in the refrigerator, at room temperature, 40°C, 50°C and 60°C the colour of the pulp were similar to the fresh fruits.
PEMBUATAN STARTER UNTUK EKSTRAKSI MINYAK KELAPA MURNI MENGGUNAKAN MIKROBA AMILOLITIK Elidar Naiola
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i1.801

Abstract

The thirteen isolates of amylolitic microbes had been tested their ability to extract the oil from " coconut milk" and nine of them could break the emulsion and separated the oil from the water and protein. The aim of this study was to find a starter that can be used for producing the coconut oil by using molase and "gula aer" gewang (Corypha utan Lamk.) palm sugar as the substrates.The result suggest that by using the isolates (ferm. 1 and ferm.2), "gula aer"gewang can be used as a substrate without supplemented by organic nitrogen. The starter prepared with isolate ferm. 1 containing cells of microbe about 10.2 x 10 cell/ml and prepared with ferm.2 about 9.0 - 10.2 x 10 cell/ml. After 4 weeks the amount of the cells decreased to 0.98 x 10 cell/ml and 0.90 x 10cell/ml, respectively, The amount of microbes were stable until 12 weeks.The starter conducted the fermentation processes at 40°C for 16 hours and produced the coconut oil. The extracted oil content about 85% saturated fatty acids and 42% of them was lauric acid. Another chemical component of the extracted oil were Iodine numbers, peroxide numbers and free fatty acid (FFA), they were 5.98%, 2.51 Meq/kg and 0.41%, respectively.
EVALUASI ANTIBAKTERI DAN ANTIOKSIDAN EKSTRAK SMILAX spp. DARI PULAU ENGGANO Praptiwi Tiwi; Kartika Dyah Palupi; Ahmad Fathoni; Ary P Keim; M Fathi Royani; Oscar Effendi; Andria Agusta
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2213

Abstract

Three species of Smilax spp. (Smilax macrophylla, S. odoratissima and S. zeylanica) collected from Enggano island were evaluated for their potential as an antibacterial and antioxidant. Stems and leaves of three species of Smilax spp. were extracted successively with nhexane,chloroform, ethyl acetate and methanol. Antibacterial activity was evaluated by TLC-bioautography against Eschericha. coli InaCC B5 and Staphylococcus. aureus Ina-CC B4. The antioxidant activity was analyzed by DPPH free radical activity by bioautography method. The value of minimum inhibitory concentration (MIC) and IC50 of active extracts were done by serial dilution in 96- well microplate. The results showed that 20 extracts have antioxidant activity, 13 extracts inhibited the growth of S. aureus Ina-CC B4, and 14 extracts inhibited the growth of E. coli Ina-CC B5. MIC values of active extracts against S. aureus Ina-CC B4 were in the range of 128 - > 512 µg / ml, while the values of MIC against E. coli B5 Ina-CC were > 512 µg / ml. IC50 values of extracts that has antioxidant activity were in the range of 184.11-4549.34 mg/L.
RHINO ATTACKS ON MAN IN UDJUNG KULON Walter Angst
BERITA BIOLOGI Vol 1, No 3 (1971)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v1i3.1443

Abstract

Reports of attacks by Javanese rhino (Rhinoceros sandaicus are quite rare. HOOGERWERF (1938) and SODY (1959) mentioned some cases, and SCHENKEL & SCHENKEL-HULLIGER (1969) also provided a general discussion of the aggressive behaviour of rhinos.
DAMPAK PENAMBANGAN GAMBUT TERHADAP BIODIVERSITAS IKAN DI KABUPATEN BENGKALIS, RIAU Haryono Haryono; Agus H Tjakrawidjaja
BERITA BIOLOGI Vol 5, No 3 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i3.1115

Abstract

A study on peat mining impact on fish diversity was conducted at Perawang, Bengkalis Regency,Province of Riau between 1996 and 1997.The purpose of study is to discover the diversity and potential of fishes in relation with water quality due to peat mining activities.The results recorded 17 species belonging to 14 genus and 10 families in mining areas, while in the protected areas (with 3 Watersheds) recorded 4 species in Bukit Batu, 21 species in Siak Kecil and 17 species in Kampar.Cyprinidae is dominant family in mining areas, while Belontiidae in protected areas.The water quality ranges are DO between 0.49 and 4.60 ppm, CO2 between 2.31 and 26.36 ppm, pH between 3.80 and 4.80 and turbidity 1.40 and 4.20. The potential offish found are mainly consumption and ornamental fish.
PERBAIKAN MUTU GENETIK UDANG GALAH (Macrobrachium rosenbergii) BERDASARKAN SELEKSI FAMILI Lies Emmawati Hadie; Wartono Hadie
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.491

Abstract

The base populations were composed to support selective breeding of giant freshwater prawn (Macrobrachium rosenbergii). Composite population were improved additive and dominance of genetic variance, especially for character of economic important. Economic character of giant freshwater prawn have a ratio of carapace length to standard length (ratio CL/SL).This research is aimed to improved the genetic of carapace and standard length ratio for giant freshwater prawn based on family selection. Selection methods were conducted on trait of carapace and standard length ratio as an edible portion. The base population prawn were used from three location i.e. Cimanuk (Cape of Air, West Java), Citanduy (Pamarican,West Java) and Musi River (Palembang, South Sumatra). Family selection was used selectedstructure. Parents were selected based on breeding value. Natural spawning was used product to first generation (F1) production of population. Larval rearing was used clear water system, fingerling production in the concrete tank, and juvenile rearing was conducted on earthen ponds. Respons selection was estimated to five months of freshwater prawn. Result of this experiment indicated that population of giant freshwater prawn can build by breeding program with heritability value of dressing out to 0.56+0.07; selection differential to 13.74 and selection intensity to 4.05. Prediction of genetic improvement from that genetic parameters is a value 7.69% to one generation. Implementation of population would be increased of genetic quality and there is decreased of gen degradation to prawn population.
AKTIVITAS LARVISIDAL EKSTRAK ETIL ASETAT DAN HEKSANA DARI FILTRAT Beauveria bassiana TERHADAP Aedes aegypti I Nyoman Pugeg Aryantha; Wahyu Setyaji Dwiantara
BERITA BIOLOGI Vol 18, No 3 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i3.3618

Abstract

Beauveria bassiana produces several metabolites that are toxic to insects so that it can be used as a biological insect control agent as an alternative to synthetic pesticides. The aim of this study was to determine the larvicidal activity of ethyl acetate and hexane extract from B. bassiana filtrate culture against Aedes aegypti 2nd instar larvae. This research was it cooked by determining the optimum age of spore inoculum of B. bassiana on the Potato Dextrose Agar (PDA) based on the number of spores and its viability. Afterwards, we determine the incubation time of B. bassiana in the Potato Dextrose Broth (PDB) in order to obtain filtrate culture which have highest mortality effects against Ae. aegypti 2nd instar larvae. B. bassiana filtrate culture was extracted with hexane and ethyl acetate and tested aegypti for larvicidal activity with a concentration range of 50, 100, 200, 300 ppm. The LC50 value was carried out by probit analysis. The results showed that ninth day old culture in the PDA was the optimum age of spore inoculum with the spore number and viability were 2.54 x 107 spore/mL and 93.46% respectively. The filtrate of sixth day old culture in PDB medium gave 100% mortality against 2nd instar Ae. Aegypti larvae. LC50 values of ethyl acetate and hexane extract were 117.28 dan 287.09 ppm. These results showed that the ethyl acetate and hexane extract of B. bassiana filtrate culture have biopesticide potential against 2nd instar Ae. aegypti larvae.   
PROSES NITRIFIKASI OLEH KULTUR MIKROBA PENITRIFIKASI N-Sw DAN ZEOLIT Dwi Agustiyani; Hartati Imamuddin; Edi Gunawan; Latifah K Darusman
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i5.1904

Abstract

The addition of zeolite into the mix culture of nitrifying bacteria N-Sw was investigated in order to improve the nitrification activity. In this experiment, the ammonium conversion was investigated by zeolite as a sole agent and also mixed with nitrifying culture N-Sw.The mix culture of nitrifying bacteria N-Sw was developed from the sludge of wastewater treatment of palm oil industry, which acclimated by ammonium sulfate for about one year. The result show that the nitrification efficiency on the treatment using nitrifying culture N-Sw was 30.76%, the ammonium elimination rate was 7.46 mg N-NH /L/hour.The addition of 10 g/l zeolite, increase both the nitrification efficiency (64.58%), and the ammonium elimination rate (14.0 mg N-NH /L/hour).The nitrification efficiency increased to be 100% on the second and third day operation, and the ammonium elimination rate was increased to be 22.4–22.9 mg N-NH /L/hour. From this experiment indicated that the role of zeolite on the improving the nitrification activity was as an absorbent of ammonium.

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