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BERITA BIOLOGI
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Articles 2,125 Documents
ANALISA ASAM ORGANIK DAN ASAM AMINO PADA NATTO YANG DIFERMENTASI OLEH 7 STRAIN BACILLUS NATTO JOKO SULISTYO
BERITA BIOLOGI Vol 3, No 6 (1986)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i6.1326

Abstract

JOKO SUUSTYO. 1986. Analysis of organic acid and amino acid in natto fermented by 7 strains of Bacillus natto. Berita Biologi 3 (6): 277 - 282. The ability of seven strains of Bacillus natto produced a number of organic and amino acid components in natto were investigated. Raw, submerced and autoclaved soybeans for natto contained 1.15% 0.46% and 0.77% in organic acid and 0.19%, 0.30% and 0.30% in amino acid, while after fermentation those range were 0.36 - 0.90% in organic acid and 2.86 -6.08% in amino add. During submercing, autoclaving and fermenting, citric acid component decreased down very sharply to about one hundredth of starting level. While acetic,iso-butiric and iso-valeric components increased up to 84.92%, 96.60% and 95.87%, respectively. Mean while, glutamic acid, iso-leucine,leucine andphenylalanine components of amino acid increased up to 84.36%, 90.52%, 91.67% and 95.88%, respecti
AKTIVITAS ANTIMIKROBA FLAVONOID - GLIKOSIDA HASIL SINTESIS SECARA TRANSGLIKOSILASI ENZIMATIK Yati Sudaryati Soeka; Elidar Naiola; Joko Sulistyo
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i6.825

Abstract

Flavonoid-glycoside was synthesized enzymatically using CGT-ase (EC.2.4.1.19) of indigenous Bacillus licheniformis in a phosphate buffer pH 6.0 at 45°C for 24 h, through transglycosylation reaction in the present of flavonoid those were extracted from rhizomes such as ginger, flngerroot, turmeric, white turmeric and white curcuma as natural acceptors, and commercial rice,cassava, corn and wheat flour as substrates.The result showed that CGT-ase of B. licheniformis transferred a glycosyl moiety in a bilayer enzymatic reaction system of n-hexanol and phosphate buffer yielding glycosides as transfer products in the present of wheat flour as substrate and white curcuma extract as its acceptor.An inhibitory effects of the synthesized flavonoid glycosides against microbial growth was furthermore examined. It was found that flavonoid-glycoside, as the transfer product, exhibited high antimicrobial activity at MIC 200 ppm on the growth of Bacillus subtilis, Escherichia coli and Saccharomyces cerevisiae, however no effect when it was assayed on Candida tropicalis, while arbutin and flavonoid-aglycon showed very low inhibitory activity on the growth of two out of four tested microbial strains.
FAUNA IKAN AIR TAWAR DI PERAIRAN KAWASAN GUNUNG SAWAL, JAWA BARAT, INDONESIA Haryono Haryono
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2186

Abstract

This study aimed to assess fish diversity and its related aspects in the Sawal Mountain Region, the watershed Citanduy in Ciamis, West Java, Indonesia. The study was conducted by grouping into four zones based on forest condition and the order of the river. The fisheswere sampled by using elektrofishing. This study recorded 12 species which Barbodes binotatus is the most abundant and widely local distributed, i.e. 20.09 individu/station and occupied 78.60 % of the area. Based on species status, as much as 75% species have wide geography distribution (common species) and the rest was introduced species. As much as 50% (6 species) have the potency as consumption fish, 25% (3 species) as ornamental fish, 17% (2 species) both as ornamental and consumption, and 8% (1 species) is stil unknown for the benefit.
POTENSI SUMBERDAYA AIR PROPINSI NTT SEBAGAI PENUNJANG PENGEMBANGAN KAWASAN CENDANA MR Djuwansah; EPU tomo; TP Sastramihardja N
BERITA BIOLOGI Vol 5, No 5 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i5.1467

Abstract

Kelayakan budidaya cendana (Santalum album L) di Propinsi NTT dibahas dari sisi sumberdaya air. Propinsi NTT merupakan daerah dengan iklim kering dibandingkan dengan propinsi lain di Indonesia. Di Propinsi ini terdapat daerah-daerah yang memiliki neraca air tahunan defisit. Daerah-daerah dengan potensi sumberdaya air yang memadai sangat terbatas. Potensi air tanah relatif sedikit dan mahal sehingga eksploitasinya hanya akan menguntungkan apabila komoditi yang diusahakan memiliki nilai ekonomis tinggi. Cendana merupakan salah satu alternatif karena (1) merupakan tumbuhan endemik daerah NTT, (2) toleran terhadap iklim kering dan (3) memiliki nilai ekonomis tinggi. Cendana laik dikembangkan secara estate di Propinsi NTT pada daerah beriklim kering yang terletak di tepi sungai atau sekitar mata air yang kontinyu, sekitar embung atau diairi oleh air tanah.
ISOLASI, SELEKSI, DAN KARAKTERISASI MIKROBA PENDEGRADASI ASETONITRIL DARI LIMBAH INDUSTRI Bambang Sunarko; Adityarini Adityarini; Usman Sumo F Tambunan; Nunik Sulistinah
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i2.1151

Abstract

A number of microbes which could grow on acetonitrile were isolated and selected from industrial effluents and were studied to characterise the isolate which has the best degrading capability.Cultures were grown on mineral medium with microelements and acetonitrile was added as sole source of energy, carbon, and nitrogen.Isolate D5, identified as Corynebacteriumsp.,was able to grow on high concentration acetonitrile (up to 5 % v/v) and exhibited the highest specific growth rate (\j).When Corynebacterium D5 grew on 2 % (v/v) acetonitrile,the doubling time was 6 hours 40 minutes,the specific growth rate (p) was 0.1 h and the acetonitrile decreasing rate was 3.99 mM/h.Increasing of acetonitrile concentration would extend the doubling time, decline the maximum growth and specific growth rate (\i), and biomass production of Corynebacterium 05.The products of acetonitrile degradation by Corynebacterium D5 were acetamide, acetic acid, and ammonia.The maximum growth of Corynebacterium D5 showed when /3-aminopropionitrile was used as a substrate.
SELEKSI DAN KARAKTERISASI MIKROBA LIGNOSELULOLITIK YANG DIISOLASI DARI LIMBAH SERBUK GERGAJI SEBAGAI MEDIA TANAM JAMUR TIRAM (Pleurotus ostreatus) R Haryo Bimo Setiarto; Iwan Saskiawan
BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i1.514

Abstract

Spent Mushroom Substrate (SMS) is a composted growing medium that results from the mushroom growing process. The utilization of SMS for biofertilizer or soil conditioner would be an important point in green agriculture. The study revealed the lignocellulolytic activity from 20 isolates of Fungi and 13 isolates of Bacteria which were isolated from sawdust as a spent mushroom substrate of Pleurotus ostreatus. The selected microorganism then would be used as inocullant for the production of biofertilizer using SMS as a main material. The lignocellulolytic system consist of laccase, cellulase and xylanase activity was analyzed. The results shown that among 20 isolates of Fungi, the highest activity of laccase, cellulase, and xylanase was obtained from the isolates 2F1, 2F4 and 2F5. There was (6.153 U, 4.662 U, 3.791U) for laccase, (6.740, 3.711 U, 3.605 U) for cellulase and (6.870 U, 4.673 U, 3.773 U) for xylanase respectively for 2F1, 2F4 and 2F5. Furhtermore, the characterization of the highest lignocellulytic fungi was also conducted. The isolate 2F1 opimally grow in pH 5 at 40oC, isolate 2F4 in pH 9 at 30oC and isolate 2F5 in pH 5 at 30oC. The identification of isolated fungi are in the progress.
KAJIAN OZONISASI (O3) TERHADAP KARAKTERISTIK KUBIS BUNGA {Brassica oleracea var. botrytis) SEGAR SELAMA PENYIMPANAN PADA SUHU DINGIN Ali Asgar; A T Sugiarto; Sumartini Sumartini; D Ariani
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1947

Abstract

The aims and objectives of this study are to determine the effect of ozonization on the characteristics of cauliflower (Brassica oleraceae var.botrytis) during cold storage.The benefit of this research is to prolong the storage period of cabbage flowers with characteristic and quality be appropriate to consumers. The design used is the Randomized Block Design with 6 replications. The treatments consisted of 0 ppm ozone concentration bear leaves, 1.5 ppm ozone concentration without leaves, 1.5 ppm ozone concentration bearing leaves, and 1.5 ppm ozone concentrations without leaves.The parameters observed were weight loss, hardness, moisture content, vitamin C, heavy metals Hg and Ag,number of microorganisms, organoleptic color, freshness and appearance. The results showed that the best treatment was 1.5 ppm ozoneconcentrations with cabbage flowers and leaves. This was caused by ozone acts in sterilization (ozone can kill a variety of pathogenic microorganisms such as bacteria, viruses and fungi), deodorant (ozone eliminating odors caused by a variety of organic compounds and microorganisms), decoloration (ozone can eliminate organic color), and degradation (ozone can break down various organic compounds and heavy metals).
FLUKTUASI DAN KOMPOSISI KOMUNUAS FITOPLANKTON DI PERAIRAN MANGROVE P. DUA (TELUK BANTEN) 1985 - 1986 MULYADI MULYADI
BERITA BIOLOGI Vol 3, No 9 (1989)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i9.1294

Abstract

MULYADI. 1989. Fluctuation and composition of phytoplankton community on Dua island mangrove waters (Ban ten bay) 1985 - 1986. Berita Biologi 3(9): 445 - 449.The study was carried out form July 1985 to June 1986 and a number of 29 phytoplankton genera were recorded.Spatial distribution of each genus within the mangrove waters due to the difference of habitat tolerance.Skeletonema, Thalassiosira and Nitzschia are the genera which have wide distribution (22.5%, 17.2% and 15.2% of distribution percentage respectively).The distributions of those genera are not always followed by high abundance. The number and abundance of phytoplankton were higher during the dry season than the rainy season.The highest density of phytoplankton community was recorded in July while the lowest was in March..
PENGARUH KONSENTRASI NITROGEN DAN FOSFOR TERHADAP PERTUMBUHAN, KANDUNGAN PROTEIN, KARBOHIDRAT DAN FIKOSIANIN PADA KULTUR Spirulinafusiformis Tjandra Chrismadha; Lily M Panggabean; Yayah Mardiati
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i3.792

Abstract

An experiment was carried out to find out the optimum nitrogen and phosphorous concentration for growth and phycocyanin production in Spirulina fusiformis culture. The cultures were grown in Zarouk medium at various nitrogen and phosphorous concentrations, which were 0.0 mM N, 7.5 mM N, 15.0 mM N, 22.5 mM N, and 30.0 mM N, as well as 0 raM P, 90 mM P, 180 mM P, 270 mM P, and 360 mM P, with four replications each. The result shows that optimal growth of the alga, which is expressed in terms of the biomass yield, was achieved at nitrogen and phosphorous concentration of 7.5 mM and 270 mM, respectively.At the same time, the highest phycocyanin content was obtained at nitrogen concentration of 22.5 mM, which was 1,2% of the biomass, and phosphorous concentration of 360 mM, which was 1.1% of the biomass. According to this result, it is suggested the optimum concentration of nitrogen and phosphorous in the media of Spirulina culture for phycocyanin production is 22.5 mM and 360 mM, respectively.
EVALUASI AKTIVITAS ANTI-INFLAMASI DAN ANTIOKSIDAN SECARA IN-VITRO, KANDUNGAN FENOLAT DAN FLAVONOID TOTAL PADA TERMINALIA SPP. Tri Murningsih; Ahmad Fathoni
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2264

Abstract

Terminalia is the second largest genus of Combretaceae family, consists of 200 species distributed in the tropics and subtropics. Many species of Terminalia are well kown in traditional medicine  in several countries of Africa and Asia. In this study, bark extracts of four species of Terminalia (T. catappa, T. citrina, T. bellirica and T. macadamii) were evaluated for their in-vitro anti-inflammatory and antioxidant activity. Determination of the anti-inflammatory activity was carried out using red blood cell membrane stabilization assay and the antioxidant activity was carried out using DPPH free radical scavenging and ferric reducing power assay. Estimation of total phenolic   content was carried out using Folin-Ciocalteu reagent, while  total flavonoid content using alumminium trichloride reagent. The results showed that extract of T. catappa has the most potent anti-inflammatory activity (IC50= 97,83±0,0100 ?g/mL) and the most powerful  antioxidant activity of DPPH free radical scavenging (IC50= 21,8900 ± 0,0264 ?g/mL) and ferric reducing power (IC50= 87,1533 ± 0,04163 ?g/ mL). The weakest anti-inflammatory activity was T. citrina extract (IC50= 159,35±0,0200 ?g/mL), whereas T. bellirica extract demonstrated the weakest antioxidant activity of  DPPH free radical scavenging (IC50= 44,4867 ± 0,0153 ?g/mL) and ferric reducing  power (IC50= 140,89±0,04 ?g/mL). The extract of T. citrina has the highest content of total phenolic (84,8167 ± 0,53407 mg GAE/g) and flavonoid (23,1200 ± 1,7149 mg RE/g) while the T. macadamii extract has the lowest content of total phenolic (24,3700 ± 0,0173 mg GAE/g) and flavonoid (13,2667 ± 0,3386 mg RE/g).

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