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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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Cassythafiliformis L. (Lauraceae): PARASIT PENGINVASI FORMASI PESCAPRAE DIPANTAIBANDEALIT, TN MERU BETIRI, JAWATIMUR Sunaryo, Sunaryo
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i4.1052

Cassytha merupakan satu-satunya marga dari suku Lauraceae, yang semua jenis-jenis anggotanya hidup sebagai tumbuhan parasit. Sampai saat ini jenis yang dilaporkan terdapat di Indonesia adalah Cassytha filiformis. Jenis ini termasuk dalam kelompok parasit obligat,yang berarti bahwa dalam siklus hidupnya parasit ini memerlukan tumbuhan lain sebagai inangnya C filiformis merupakan tumbuhan parasit yang dapat menyerang berbagai jenis tumbuhan herba, semak, bahkan pepohonan.Didalam relung ekologinya parasit ini menyerang bagian-bagian batang, cabang, ranting juga bagian daun tumbuhan inangnya.Menyukai hidup didaerah terbuka dimana sinar matahari memberikan terpaan yang optimum. Didalam memparasiti tumbuhan

SIMTOMATOLOGI DAN WAKTU KEMATIAN RAYAP Macrotermes gilvus Hagen (ISOPTERA: FAMILI TERMITIDAE) SETELAHINFEKSI CENDAWAN Metarhizium brunneum Petch Sayuthi, Muhammad; Santoso, Teguh; Harahap, Idham Sakti; Kastosuwondo, Utomo
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The potential of entomopathogenic fungus Metarhizium brunneum Petch as biocontrol agent to termite Macrotermes gilvus Hagen has been tested in the laboratory.The purpose of this research is to study the symptomatology and lethal time of the termite M. gilvus fungus after infection by M. brunneum. The density of conidia at 1.21xlO6/mL showed as effective concentration in causing M. gilvus mortality, when compared to 1.08><106conidia/mL, and control. The infection stage of M. brunneum on the host until the death of its host occurred on day 2,and the sporulation of M. brunneum on the surface of the host integument occur on day 4. The entire surface of M. gilvus was covered by the mycelium and conidia of M. brunneum on day 7. Lethal time 50% of termite population of M. gilvus (LT50) was achieved in 5 days(5.14), and LT95 achieved on day 10 (10.03).

ANALISIS KERAGAMAN GENETIK Musa balbisiana Colla BERDASARKAN MARKA RAPD DAN ISSR [Genetic variation analyses of Musa balbisiana Colla based on RAPD and ISSR markers] Poerba, Yuyu Suryasari; Ahmad, Fajarudin
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Wild Musa balbisiana Colla is one of the progenitors of cultivated bananas and plantains. It is originated in Asia, and distributed from India to Papua New Guinea. This study was conducted to assess the molecular diversity of 25 accessions of M. balbisiana based on Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) analyses. RAPD and ISSR fingerprints of these banana varieties were detected by amplifications of nine primers of RAPDs and six primers of ISSRs. RAPD primers produced 84 amplified fragments varying from 150 bp to 2300 bp in size. 21.43 % of the amplification bands were polymorphic. ISSR primers produced 61 amplified fragments varying from 250 bp to 2200 bp in size. 29.30 % of the amplification bands were polymorphic. Based on these results, the 25 accessions of Indonesian M. balbisiana showed a low genetic variation, with coefficient of similarity ranged from 0.81 to 0.99.

PENGARUH MIKROBA TANAH TERHADAP PERTUMBUHAN DAN HASIL PANEN KEDELAI (Glycine max L.) Purwaningsih, Sri
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1121

A study was conducted in order to know the effect of soil microorganism as biofertilizer on the growth and yield of soybean (Glycine max L.) in Malang, East Java.The purpose of the study was to get inoculants as biofertilizer to improve the production of soybean.The experiment was carried out in Bantur village, Bantur district, South Malang, East Java, by using the rice land.The strain used were five single inoculants as follows: A (Bio 20R + Bio 22R + Bio 277R), B (Bio 277R + Bio 278R + Bio 302R), C (1L + 8L + Bio 302R), D (Bio 20R + Bio 22R + Bio 277R + Bio 278R + Bio 302R + 1L + 8L), V (MVA fungi), and four mixed inoculants (AV, BV, CV, and DV). Four controls as uninoculated plant and without fertilizer (Ki), uninoculated plant and with Urea fertilizer equal to 100 kg/ha (K2), uninoculated plant and with TSP fertilizer equal to 80 kg/ha (K3), uninoculated plant and with Urea and TSP fertilizer (K4). The experiment was designed as Randomized Complete Block as 13 treatments with three replications for each treatment, and interval planted with 20 x 35 cm. The plants were harvested after 90 days. The parameters of investigation were the dry weight of canopy, roots, root nodules, seeds, 100 of seeds, number of nodules and pods with harvest productions. The results showed that single inoculants (MVA fungi) and mixed inoculants CV (1L + 8L + Bio 302R) and MVA fungi) gave better results on the production of soybean.

PERUBAHAN KADAR KOLESTEROL SERUM PADA TIKUS SETELAH MENGKONSUMSI MALTOOLIGOSAKARIDA YANG DISINTESIS SECARA ENZIMATIK MENGGUNAKAN AMILASE Bacillus licheniformis BLl [The Change of Serum Cholesterol Level in Rats after Consuming Maltooligosaccharide Synthesized by Enzimatic Reaction of Bacillus licheniformis BLl Amylase] Dinoto, Achmad; Rahayu, Rita Dwi; Satyaningtijas, Aryani S
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

To investigate the impact of consuming enzymatically synthesized maltooligosaccharide using Bacillus licheniformis BLl amylase on reduction of serum cholesterol level, in preclinical study groups of Sprague Dawley rats were fed for 3 weeks with four different test diets: basal diet (BD), high serum cholesterol-stimulating basal diet (CD), high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) commercial maltooligosaccharide (CI), and high serum cholesterol-stimulating basal diet supplemented with 3% (w/w feed) maltooligosaccharide synthesized by enzimatic reaction of B. licheniformis BLl amylase (CB).Our result shows a reduction of serum cholesterol level up to 39.1% in rats CB after three weeks consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase. In addition, significantly increased blood glucose level was not found in this study as an impact of consuming maltooligosaccharide synthesized using B. licheniformis BLl amylase.

PATOGENISITAS Salmonella enterica SEROTIPE enteritidis ISOLAT LOKAL PADA ANAK AYAM DAN MENCIT Kusumaningsih, Ani
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i4.764

This study was to identify the pathogenicity of local isolates of Salmonella enterica serotipe enteritidis (S. enteritidis/SE) in experimental chickens and mice. Thirty six local isolates of S. enteritidis isolated from chickens, eggs and human was inoculated 8 intra peritoneally (ip) to each 5 chickens and mice per group. Each group received 0.2 ml of broth culture containing 2 x 10Ã CFU of S. enteritidis. Chickens and mice were observed daily at 1, 2, 3, 4, 5, 6 and 7 days after inoculation for clinical signs and mortality. The result showed that the overall mortality rate of chicken and mice were 78.4% and 63.9% respectively. The highest mortality rate was in the first day after inoculation, 51.1% for chickens and 40.0% for mice, however the lowest mortality rate was in the sixth day (0.0%) after inoculation in chickens and in the seventh day (0.6%) in mice. The bacteria of S. enteritidis can be isolated from various organs (liver, heart and spleen) and caeca tonsil of every dead chickens and mice. The study showed that local isolates of S. enteritidis isolated from chickens, eggs, and human were very pathogen for experimental chickens and mice.

AKTTVITAS SELULASE, AMILASE DAN INVERTASE PADA TANAH KEBUN BIOLOGI WAMENA Rahmansyah, M; Latupapua, HJD
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Enzymatic activities in soil as due to microbes action in organic matter degradation, lead to propose as indicators for determining soil degree enrichment.In this work, the enzymatic activities of cellulase, invertase and amylase were determined in tropical soil collected from Biological Research Station in Wamena. Result of measurement on five soil samples showed that cellulase activity occurred between 0.10 - 0.31 mg reducing sugar/g soil/hour in 2% Carboxymethylcellulose (CMC) substrate, and about 0.15 - 0.25 mg reducing sugar/g soil/hour in 0.5% Avicel substrate.Amylase and invertase assayed in 6% of amylum and 6% of sucrose substrates correspondingly; and its activities between 2.55 - 3.54 and 0.59 - 1.30 mg reducing sugar/g soil/hour, respectively. Correlation of enzymatic activities and soil organic-C content (1.35 - 2.70%) of soil layer was significant in selulase and amilase, but it was poor correlation in invertase activity.

DIVERSITY OF XYLOSE ASSIMILATING YEAST FROM THE ISLAND OF ENGGANO, SUMATERA, INDONESIA [Keragaman Khamir Pengguna Xilose yang Diisolasi dari Pulau Enggano, Sumatera, Indonesia] Kanti, Atit; Sumerta, I Nyoman
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Naturally occurring yeasts (wild type) are important genetic resources for many industrial interests. Though biodiversity exploration of Enggano has been done since 1944, but there is no report for yeast diversity from this island. Due to its industrial interest, xylose fermenting yeast is target for many scientists. This present study aims to isolate xylose assimilating yeast from island of Enggano. The samples were collected from varying sources included soil, leaf litter, decay wood, fruit and sediment. Xylose enrichment culture technique was performed to enrich xylose fermenting yeast. While for the isolation of yeast several methods which include dilution, ballistopore falling, direct isolation, membrane filtration were done. Of 200 isolates, 76 strains (38 %) were xylose assimilating yeast which mostly belong to Candida which include C. insectorum, C. tropicalis, C. boidinii, C. pseudolambica, C. yuanshanica, C. silvae, Cyberlindnera saturnus, Williopsis saturnus and Sporobolomyces poonsookiae. One strain Candida sp. that was isolated from soil could be assigned as a candidate of novel species on base on its morphological and biochemical characteristics along with analyses of gene sequence from D1/D2 domain. This study confirms that yeast survey is very important to obtain new genetic resources for industrial interest as well as for taxonomic study.

PENAPISAN AKTIVITAS LIPOLITIK SEPULUH BIAKAN Rhizopus KOLEKSIUICC (UNIVERSITY OF INDONESIA CULTURE COLLECTION) Lusini, Inu Yuyun; ET, Wibowo Mangunwardoyo; Gandjar, Indrawati
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i6.849

Ten Rhizopus were screened for their extracellular lipolytic activity. All strains showed lipolytic activity with diffferent activities.R. microsporus var. rhizopodiformis (Cohn) Scipper & Stalpers UICC 520 (4.52 unit/ml) and R. microsporus var. oligosporus UICC 550 (2.58 unit/ml) showed a high lipolytic activity in screening medium containing 5% pepton and 1% glucose (b/v) without a lipid substrate after 24 hours of incubation at room temperature.

PEMANFAATAN INOKULAN MIKROBA SEBAGAI PENGKAYA KOMPOS PADA BUDIDAYA SAYURAN [Microbial inoculants for compost enrichment on vegetables cultivation] Antonius, Sarjiya; Rahmansyah, Maman; Muslichah, Dwi Agustiyani
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Â Microbial existence is due to mineralize organic compound in soil and caused nutrients availability to the plants. In this study, some of microbial inoculants consist of a). Phosphate soluble bacteria (Pseudomonas sp.) collected from soils that intensively utilized agrochemical in its agriculture activities; b). Nitrogen - fixing bacteria Rhizobium leguminosarum; and c) Trichoderma sp. and Aspergillus sp.as organic degradation fungi were used for compost enrichment.These inoculants formulation were utilized to carrots (Daucus carota L.), broccoli (Brassica oleracea L.) and maize (Zea mays L.) cultivation in upland area, Cisarua, Bogor, West Java. This study was aimed to assess inoculants response to enrich fully mature and half ripe compost. Crops response to the inoculation treatment was varied among these commodities. In general, the response of plants with the use of compost enriched biofertilizer was positive, with an increase in yield of about 15- 30% for carrots, broccoli around 65- 90% and about 10% of corn crops. The significant increase in crops production as impact of organic fertilizier application indicated that C organic content of the organic farm of Cimelati and Agato were defisit.

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