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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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PENGGUNAAN VAKSIN Aeromonas hydrophila: PENGARUHNYATERHADAP SIANTAN DAN IMUNITAS LARVA IKAN PATIN (Pangasionodon hypophthalmus) Lusiastuti, Angela Mariana; Hadie, Wartono
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Aeromonas hydrophila is a pathogen that often causes considerable losses in the area of freshwater fish fanning. Vaccination is one way to simulate parent catfish make specific immunity. Specific immunity generated by the parent will be forwarded through the oocytes produced during a certain time span. The aim of this research was to know the effect and the effectivity of using hydrovac vaccine with and without the complete adjuvant. This research was done on Patin fish Pangasionodon hypophthalmus whose givng Hydrovac 0.4 ml/kg of body weight. The comparation between complete adjuvant and vaccine was 1:1. Injection was done by intra peritoneal for three mothers each with and without complete adjuvant. Injection was done at gonad maturity level II. The result showed that antibody were positively detected on mother serum which used adjuvant or not. On larva stage, antibody was detected until four weeks old. While on 2 weeks old of larva, the concentration of titer antibody was very high and raised the dilution of 1: 2048. Survival rate of juvenile which their mother got a vaccine raised 93%, was better than 73%-63% using mother without vaccine. Booster immersion of hydrovac vaccine could give preferably at the end of three weeks old or in the beginning of fourth weeks old of larva.

PERGERAKAN LUTUNG JAWA Trachypithecus auratus (E. Geoffroy 1812) PADA FRAGMEN HABITAT TERISOLASI DI TAMAN WISATA ALAM GUNUNG PANCAR (TWAGP) BOGOR [The Movement of Javan Langur Trachypithecus auratus (E. Geoffroy 1812) in Isolated Habitat Fragment in TWAGP Bogor] Sulistyadi, Eko; Kartono, Agus Priyono; Maryanto, Ibnu
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Javan langur is protected spesies of primates that highly threatened. Habitat degradation as a major threat causes javan langur Trachypithecus auratus (E. Geoffroy 1812) populations living in isolated habitat fragments. The research was carried out to determine the daily movement of javan langur in TWAGP as form of adaptation in isolated habitat fragments. The study was conducted in May-June 2012 by using focal animal sampling method. Vegetation analysis was performed with a point centered quarter method. The results showed that there were three major land cover types used by the Javan langur, i.e. forest, transitional forest-cultivation and cultivation. The dominant activity occurred in transisional forest-cultivation habitat (48.19%). The highest proportion of activity wass resting (33.65%) followed by feeding (30.68%), moving (27.08%) and social activities (8.60%). Based on the variations of vegetation strata, the dominant activity performed at stratum C(4-20 meters) accounted for 80.07%.

PENGGUNAAN MODEL DENGAN PROGRAM CSMP UNTUK MENDUGA POTENSI HASIL PADI BERDASARKAN PENDEKATAN IKLIM, TANAH DAN TANAMAN Estiningtyas, Woro
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Most of rice yield potential prediction models are focussed on optimization of two factors(i. e. soil and crop factors) than another(climate).It is probably due to significant influence of soil and crop factors thananother in rice production.In fact,the result of yield prediction taking into account soil and climate is not sufficient because climate will became limiting factor.In order to integrate climate with soil and crop factors in rice yield potential prediction,the CSMP(Continuous System Modelling Program) model is used in this research.The results of simulation shows that through climate optimization, actual rice yield production can be increased 1500-2000 kg more than actual production.Yield potential during one year can be optimised.Moreover,CSMP can give suggestion in.the efficient use of natural ressources such as nitrogen, phosphor and potassium.

MAKNA NILAI PENTING BUDAYA KEANEKARAGAMAN HAYATI TUMBUHAN BAGI MASYARAKAT DI TAMAN NASIONAL KERINCI SEBLAT DI KABUPATEN KERINCI, PROPINSI JAMBI [The Importance of Cultural Significance Index of Plants Diversity For The Communities Within The Kerinci Seblat National Park, Kerinci Regency, Province of Jambi] Helida, Asvic; Zuhud, Ervizal A.M.; Hardjanto, Hardjanto; Purwanto, Y.; Hikmat, Agus
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Index of Cultural Significance biodiversity plants in ethnobotanical study was an important step for seeking a strategy for subsisten activity. This study was aimed to identify utilised plants that have the highest value at Kerinci community. The current study was conducted in three villages within the Kerinci Regency, Jambi Province, which located close to the Kerinci Seblat National Park: Baru Lempur, Lama Tamiai, and Ulu Jemih for 8 months (October 2013 to May 2014). Data was obtained by implementing Â participation-observation method through interviews. Estimation of cultural significance for every plant species observed using the Index for Cultural Significance (ICS). Results indicated that the people of Kerinci possessed good knowledge on the biodiversity of their surrounding. Two hundred and thirty four species of useful plants we recorded with ICS value ranging from 3 to 59. Rice (Oryza sativa; Poaceae) and Cinnamon (Cinnamomum burmannii; Lauraceae) had highest ICS value, whereas âingguâ (Ruta angustifolia; Rutaceae) and âbunching onionâ (Allium fistulosum, Amaryllidaceae ) have the lowest ICS value.

PERKEMBANGAN OOSIT IKAN PATIN SIAM, Pangasianodon hypophthalmus Sauvage, 1878 (PANGASIIDAE; SILURIFORMES) Tahapari, Evi; Iswanto, Bambang
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pangasianodon hypophthalmus is an introduced species of fish culture and fisheries resources in Indonesia. Reproductive biology aspects, e.g. maturity, plays an important role on the fisheries management. There was no detail information of oocytes development of P. hypophthalmus. The aim of this study is to find out the detail of its oocytes development through the observation of whole oocytes and ovarian histological slides microscopically. The results showed that the rhythm of oocyte development of P. hypophthalmus was grouped synchronism, assigned by the presence of two oocyte groups in the mature females, i.e. the large and maturing oocytes which would be spawned soon, and the small ones as the stock and still unyolked for the next spawning. The oocyte development could be devided into five stages, i.e. stage 1 (chromatin nucleolar and perinucleolar) with oocytes diameter less than 300 urn, stage 2 (yolk vesicles and cortical alveolar) with oocytes diameter of 230-660 urn, stage 3 (yolk granules) with oocytes diameter of 430-1,100 urn, stage 4 (migratory nucleus and hydrated) with oocytes diameter of 950-1,260 urn, and stage S (atretic) with oocytes diameter less than 800 m

PEMANFAATAN KEANEKARAGAMAN SUMBERDAYA TUMBUHAN OLEH MASYARAKAT BADUY-DALAM DI SEKITAR GUNUNG KENDENG SELATAN, KABUPATEN LEBAK, BANTEN BAGIAN SELATAN Wardah, Wardah
BERITA BIOLOGI Vol 6, No 6 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Kendeng mountain, where "Baduy-Dalam" community lives is a native reserve area.Observation around "leuweung kolot","leuweung ngora", and "leuweung lembur" near the community area shows how the Baduy-Dalam community controls the sustainability of their forests.These forests are almost untouchable.Information on the biodiversity of plant species in the areas have not known yet.A study on the knowledge of Baduy-Dalam community and utilization of plant resources in Cikeusik,Cikertawana, and Cibeo villages was conducted.The results shows that the Baduy-Dalam tribe had a close relationship with their surroundings; it is seen from their knowledge in managing the environment in order to survive and continuing their living.Among 163 plant species recorded, 81 species are as potential food plants (the largest group), 38 species of medicinal plants, 17 species of building materials, 8 species for ritual, 17 species for fire wood, 4 species handycraf, 3 species economically potential, and 1 species for drinking. There are two species is considered as endangered species namely Arcangelisia Jlava and Alstonia scholaris.

VARIASI DAN DEGRADASI SUARA PANGGILAN KODOK JANGKRIK [HYLARANA NICOBARIENSIS (STOLICZKA, 1870)] ASAL PULAU ENGGANO (ANURA: RANIDAE) [Variation and degradation on advertisement calls of Cricket Frog, Hylarana nicobariensis (Stoliczka, 1870) from Enggano Island] Kurniati, Hellen; Hamidy, Amir
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Frogâs group of Hylarana nicobariensis (Stoliczka, 1870) is a complex species that has diverse genetic populations and variations on advertisement calls. Variations on advertisement calls among populations have been reported in Sumatra, Java and Bali. The population of frog in Enggano has different phenomenon that was not found in Sumatra and Java. Â Enggano population was genetically identical, but the advertisement calls were very diverse. There were four types of advertisement calls on the population in Enggano Island, namely: (1) advertisement call type 1 which has pulse wave with the dominant frequency of 2855.13 Â± 135.22 Hertz; (2) advertisement call type 2 which has pure tone wave with the dominant frequency of 2720.29 Â± 97.69 Hertz; (3) advertisement call type 3 which has pulse wave with dominant frequency 2634.61 Â± 292.81 Hertz; (4) advertisement call type 4 which has pure tone wave with dominant frequency 1618.79 Â± 83.05 Hertz. The degradation of the dominant frequency and the lower frequency was likely due to the increase of snout to vent length (SVL) of adult male that were followed by increasing the size of the head.

MIKROBAENDOFITIK DARI TAMAN NASIONALBATANG GADIS SUMATERA UTARA: POTENSINYA DALAM MENGHASILKAN SENYAWA ANTIMIKROBATERHADAPMIKROBAPATOGEN Sukiman, Harmastini; Lekatompessy, Sylvia; Widowati, Tiwit
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Batang Gadis National Park (TNBG) is one of the tropical forests in North Sumatra with megabiodiversity of flora and fauna including microorganism. Endophytic microbes conservation from varieties of forest plants in Batang Gadis National Park has been completed. Nineteen endophytes isolates from TNBG have been screened for their potential on producing bioactive compound against the pathogenic bacteria. Qualitative screening has been done using the growth agar media and identified the clear zone appeared surrounding the bacteria colony. The result indicated that isolate MSCI 87.4 showed high strengthening secresion (4.35) againts Xanthomonas campestris, whereas isolate MSCI 37.1 showed high secretion against Bacillus subtilis (2.69) and Escherichia coli (2.60). Isolate MSCI 37.4 showed potential on producing bioactive compound againts Staphylococcus aureus (4.41). Isolates MSCI 87.4, MSCI37.1, MSCI 37.4 and MSCI 58.1 even could produce bioactive compound against four pathogenic bacteria that are Xanthomonas campestris, Bacilus subtilis, Escherichia coli and Staphylococcus aureus, whereas isolate MSCI 15.5b potential on producing bioactive compound against two isolates namely Xanthomonas campestris and Bacillus subtilis. The endophytes bacteria mainly belong to the Gram negative group and four out of nineteen isolates tested belong to the Gram positive group. The cell mainly coccus and only one is bacilli without fiagella. The five most potential isolates has been maintained under freeze dried condition for futher conservation and study. Thin Layer Analysis using semipolar organic separation solution showed that most isolates identified were able to produce bioactive compound except two isolates i.e. MSCI 48.4a and MSCI 53.1; however further analysis is needed to confirm the product.

RESPON PADI TRANSGENIK CV. NIPPONBARE GENERASI T1 YANG MENGANDUNG GEN Oryza sativa DEHYDRATION-RESPONSE ELEMENT BINDING 1A (OsDREB1A) TERHADAP CEKAMAN SALINITAS [Response of T1 Generation Transgenic Rice cv. Nipponbare Containing an Oryza sativa Dehydration-response Element Binding 1A (OsDREB1A) Gene to Salinity Stress] Santoso, Tri Joko; Apriana, Aniversari; Sisharmini, Atmitri; Trijatmiko, Kurniawan Rudi
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Salinity is one of the abiotic constraints in the cultivation of rice crop. One of the reasons agricultural land becomes saline is due to the intrusion of seawater into the mainland as a result of global climate change. Dehydration-responsive element binding (DREB) gene is a plant -specific transcription factor gene that have important role in regulating plant responses to abiotic stresses, including high salinity. Transgenic rice plants cv. Nipponbare carrying OsDREB1A gene have been generated. However, study of the response of putative transgenic plants to salinity has not been done. The research objective is to study the response of T1 generation Nipponbare-OsDREB1A transgenic rice plants to salinity stress. The result showed that the response of putative transgenic rice Nipponbare-OsDREB1A to salinity stress 25 mM and 150 mM NaCl indicated a level of tolerance varies from highly sensitive to highly tolerance. These variations were possibly occurred because of the segregation state of the T1 generation transgenic rice. Based on damage symptom scoring and PCR analysis provided information that transgenic rice plant cv. Nipponbare-OsDREB1A which showed positive PCR had a very high tolerance to salinity stress 150 mM compared with non-transgenic rice cv. Nipponbare.

VARIASI GENETIK Lactobacillus fermentum Beijerink ASAL SAYUR ASIN BERDASARKAN ANALISIS RFLP 16S-23S rDNA ISR, RAPD-PCR DAN ERIC-PCR [Genetic Variation of Lactobacillus fermentum Beijerink Origin Sayur Asin Based on RFLP 16S-23S rDNA ISR, RAPD-PCR and ERIC-PCR Analysis] Sulistiani, Sulistiani; Mangunwardoyo, Wibowo; Abinawanto, Abinawanto; Sukara, Endang; Dinoto, Achmad; Salamah, Andi
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Molecular analysis of Lactobacillus fermentum isolates is essential to understand their genetic variation in relations to their roles in sayur asin fermentation process. Combination of three molecular techniques which is restriction fragment length polymorphism (RFLP) of 16S23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA (RAPD-PCR) and an enterobacterial repetitive intergenic consensus (ERIC-PCR) analysis were performed to discriminate 19 representative isolates of L. fermentum isolated from sayur asin. The result showed that L. fermentum strain D11 is distantly related to other isolates based on RFLP using HhaI restriction enzyme and RAPDPCR analyses. In addition, both of RAPD-PCR and ERIC-PCR successfully determined the genetic variation among L. fermentum strains by exhibiting distinct 4-8 bands (800-2080 bp) and 4-10 bands (280-3050 bp), respectively. A dendogram generated from UPGMA cluster analysis of both RAPD-PCR and ERIC-PCR data showed two distinct genotypic groups exist among L. fermentum isolated from sayur asin in Indonesia.

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