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BERITA BIOLOGI
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Articles 2,125 Documents
LINNAEUS DAN SAYA Rifai, Mien A
BERITA BIOLOGI Vol 8, No 4(a) (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.172 KB) | DOI: 10.14203/beritabiologi.v8i4(a).1536

Abstract

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KRIOPRESERVASI TANAMAN PURWOCENG {Pimpinella pruatjan Molk.) DENGAN TEKNIK VITRIFIKASI Roostika, I; Darwati, I; Megia, R
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.165 KB) | DOI: 10.14203/beritabiologi.v8i6.821

Abstract

Pruatjan (Pimpinellapruatjan Molk.) is an Indonesian endangered medicinal plant that included in Appendix I based on CITES. Therefore it is a highly protected species. To avoid extinction of this plant, it is very important to conserve the plant. In vitro conservation is more suitable since this plant is difficult to be cultivated outside of its habitat. Cryopreservation technique may conserve this material for a long-term period. The objectives of this research were to find optimized treatments for pre culture, loading, and dehydration on cryopreservation of pruatjan. The research was conducted at Tissue Culture Laboratory in Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, started from May to November 2007. Pre culture was conducted using DKW basal media that added by sucrose at the level of 0.3,0.4, and 0.5M for one and three days incubation. Loading was conducted in DKW basal media containing 2M glycerol and 0.4M sucrose for 15,30, and 45 minutes duration time. Dehydration was conducted in several cryoprotectants, namely PVS1 (22% glycerol + 13% propy lene glycol + 13% etylene glycol + 6% DMSO + 3% sucrose), PVS2 (30% glycerol + 15% etylene glycol + 15% DMSO + 0,4M sucrose), PVS3 (50% glycerol + 50% sucrose), and PVS4 (35% glycerol + 20% etylene glycol + sucrose 0.6M). Result showed that pruatjan could be preserved through cryopreservation by vitrification method. The best pre culture was using 0.3 M sucrose for one day, the best loading was 30 minutes, while the best cryoprotectant was PVS2 with 90% success before freezing and 40% after freezing. The success may be improved by applying pre growth treatment, optimizing temperature of thawing, modification of recovery media and incubation condition.
GENETIC ANALYSIS OF SOME SPECIES OFPinanga (PALMAE) BY USING ISSR MARKERS Witono, Joko Ridho; Rondo, Katsuhiko
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i1.812

Abstract

A genetic analysis of 13 species of Pinanga (Palmae) was conducted by using Inter Simple Sequence Repeat (ISSR) markers. The markers were used in this study belonged to UBC primer set #9 (UBC 801-900) and each primer contains 15 to 22 mer nucleotides.Based on primer screening, nine UBC primers had clear and reproducible polymorphism bands. According to Dices and Jaccards similarity coefficients, cluster analysis by UPGMA among the 13 Pinanga species showed two clusters. Cluster A consisted of nine species: P. javana, P. arinasae, P. patula, P. salicifolia, P. coronata, P. scortechini, P. disticha, P. grandis and P. densiflora; and cluster B consisted of four species from five accessions: P. caesia, P. copelandi, P. rumphiana-1, P. rumphiana-2 and P. insignis. The genetic similarity among the 13 species of Pinanga had a correlation with their geographical distribution. In cluster A, all of the accessions are from Sundaland and the adjacent region of Thailand, whereas all of the accessions in cluster B were distributed in the Philippines, Wallacea, and the New Guinea regions. Possibly this genetic similarity was caused by their geographical history and the natural barriers between them. This is the early conclusion was conducted using genetic markers on Pinanga. Further studies such as sequencing (plastid and nuclear ribosomal DNA) and applying more accessions of Pinanga species from broader geographic distributions may provide a better understanding of the relationships. The 1SSR markers application is a simple and quick way to analyze genetic relationships because no prior sequence data is needed, a large number of markers can be generated, and the supplies and equipment required are minimal.
EPIFIT DAN LIANA PADA POHON DI HUTAN PAMAH PRIMER DAN BEKAS TERBAKAR KALIMANTAN TIMUR, INDONESIA SIMBOLON, Herwint
BERITA BIOLOGI Vol 8, No 4 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1190.34 KB) | DOI: 10.14203/beritabiologi.v8i4.2115

Abstract

Epiphytes and lianas on the trees grown in three categories of gradation of Mixed Dipteroearp Forests (natural, lightly degraded and heavily degraded after forest fires) have been studied in Bukit Bangkirai Nature Recreation Park, East Kalimantan.166 species of epiphytes and lianas were found in those three study plots, among them 89; 134 and 56 species were distributed in the natural, lightly degraded and heavily degraded forest plots, respectively. 30 species were distributed widely in three types of forests, 37 species were tend to be the shade tolerant species and 16 species as light demanding species, since they were distributed in the closed and open forests, respectively. The most common species that distributed in those three studied plots were Derris elegans, Spatholobus gyrocarpus and Embelia ribes. Number of epiphytes and lianas on the single individual tree host were increase as the tree diameter increased. Number of species of epiphytes and lianas on a species of host were increased as the number of individuals were increased, indicates that most of these epiphytes and lianas were not host specific.
PENGARUH KONSENTRASI NITROGEN DAN FOSFOR TERHADAP PERTUMBUHAN, KANDUNGAN PROTEIN, KARBOHIDRAT DAN FIKOSIANIN PADA KULTUR Spirulinafusiformis Chrismadha, Tjandra; Panggabean, Lily M; Mardiati, Yayah
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (430.143 KB) | DOI: 10.14203/beritabiologi.v8i3.792

Abstract

An experiment was carried out to find out the optimum nitrogen and phosphorous concentration for growth and phycocyanin production in Spirulina fusiformis culture. The cultures were grown in Zarouk medium at various nitrogen and phosphorous concentrations, which were 0.0 mM N, 7.5 mM N, 15.0 mM N, 22.5 mM N, and 30.0 mM N, as well as 0 raM P, 90 mM P, 180 mM P, 270 mM P, and 360 mM P, with four replications each. The result shows that optimal growth of the alga, which is expressed in terms of the biomass yield, was achieved at nitrogen and phosphorous concentration of 7.5 mM and 270 mM, respectively.At the same time, the highest phycocyanin content was obtained at nitrogen concentration of 22.5 mM, which was 1,2% of the biomass, and phosphorous concentration of 360 mM, which was 1.1% of the biomass. According to this result, it is suggested the optimum concentration of nitrogen and phosphorous in the media of Spirulina culture for phycocyanin production is 22.5 mM and 360 mM, respectively.
PROSES NITRIFIKASI OLEH KULTUR MIKROBA PENITRIFIKASI N-Sw DAN ZEOLIT Agustiyani, Dwi; Imamuddin, Hartati; Gunawan, Edi; Darusman, Latifah K
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.491 KB) | DOI: 10.14203/beritabiologi.v8i5.1904

Abstract

The addition of zeolite into the mix culture of nitrifying bacteria N-Sw was investigated in order to improve the nitrification activity. In this experiment, the ammonium conversion was investigated by zeolite as a sole agent and also mixed with nitrifying culture N-Sw.The mix culture of nitrifying bacteria N-Sw was developed from the sludge of wastewater treatment of palm oil industry, which acclimated by ammonium sulfate for about one year. The result show that the nitrification efficiency on the treatment using nitrifying culture N-Sw was 30.76%, the ammonium elimination rate was 7.46 mg N-NH /L/hour.The addition of 10 g/l zeolite, increase both the nitrification efficiency (64.58%), and the ammonium elimination rate (14.0 mg N-NH /L/hour).The nitrification efficiency increased to be 100% on the second and third day operation, and the ammonium elimination rate was increased to be 22.4–22.9 mg N-NH /L/hour. From this experiment indicated that the role of zeolite on the improving the nitrification activity was as an absorbent of ammonium.
PRODUKSI KITINASE EKSTRASELULER Aspergillus rugulosus 501 SECARA OPTIMAL PADA MEDIA CAIR Widhyastuti, Nunuk
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.982 KB) | DOI: 10.14203/beritabiologi.v8i6.839

Abstract

Chitinases hydrolyzing chitin are produced by various organisms and their physiological functions depend on their sources.Chitinases and its hydrolysis products have a broad range of applications. Increasing commercial interest in chitinase and its products has led to the need of inexpensive, reliable sources of active and stable chitinase preparations.The activity of chitinase produced by Aspergillus rugulosus 501 in liquid culture and the parameters that controlled the enzyme synthesis were studied to find optimal conditions for the enzyme production. Hydrolysis products of chitinase were detected by Reissig method. The results showed that fungal chitinase activity in media containing organic nitrogen and phosphate was higher than that in media containing trace element, organic and anorganic nitrogen but lack of phosphate. The maximum enzyme activity reached in the second day incubation.Chitinase production was affected by the initial pH of media, chitin consentration and source of nitrogen, while the consentrasion of inoculum used in this study did not affect enzyme production. High enzyme activity was found in media with initial pH 5.0-6.5. When using different consentration of colloidal chitin, chitinase activities were also found high in concentration from 0.75% tol .5%. The organic nitrogen sources gave the better results compared to the anorganic ones.
PERBANYAKAN ULIN (Eusideroxylon zwageri T.et.B) DENGAN BUI DAN SETEK Utami, Ning Wikan; Hoesen, Djadja Siti Hazar; Witjaksono, Witjaksono; Danu, Danu
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.393 KB) | DOI: 10.14203/beritabiologi.v7i4.2064

Abstract

The ulin or so called vernacular name kayu besi (Eusideroxylon zwageri T.et.B.) is one of the the important timber in East Kalimantan (East Borneo).However, there are some problems in the regeneration of this species. The research on propagation of ulin was carried out in Treub Laboratory, Research Center for Biology. The research consist of two experiments The research consisted of two experiments.The first experiment seeds which was treated in order to stimulate the germination and the second experiment by treating the mixture between 1BA and vitamin C for stimulating root cutting.The best result of seeds germination was recorded 90%,which the seeds were treated by removing the whole seedcoat and then by exposing to the sunlight for 5 hours The seed itself began to germinate 7 weeks after sowing.Meanwhile, the control no germination et all until 28 weeks.The best result on cutting was indicated by treating with the combination of IBA 10 mg/1 and vitamin C 50 mg/1 which was achieved the highest rooting 100%,although without hormone treatment only resulted 33.3% rooting.
FAUNA IKAN DI SUNGAI CIMADUR, CIUJUNG DAN CIBERANGDI SEKITAR TAMAN NASIONAL GUNUNG HALIMUN, KABUPATEN LEBAK, BANTEN Rachmatika, Ike; Dewantoro, G Wahyu; Sauri, S
BERITA BIOLOGI Vol 7, No 1&2 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i1&2.1233

Abstract

Fish fauna survey were conducted in Cimadur, Ciujung and Ciberang Rivers. From these studies six fishes species were found as additional record for fishes living in Gunung Halimun National Park and its adjacent area. This finding brings the fish species living at this park to be sixty.In this record, it includes Cypriniid fishes that attain large size: Barbodes balleroides and Osteochilus hasseltii, found in the segment of the middle of R. Ciujung (18.3 m asl).
GENETIC VARIABILITY AND HERITABILITY ESTIMATES OF QUANTITATIVE CHARACTERS IN LOCAL MUNGBEAN (Vigna radiata (L.) Wilczek) VARIETIES [Keragaman Genetik dan Dugaan Heritabilitas Karakter Kuantitatif pada Varietas Lokal Kacang Hijau (Vigna radiata (L) Wilczek Hakim, Lukman
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (172.722 KB) | DOI: 10.14203/bb.v8i5.1893

Abstract

Information of the variability and heritability of quantitative characters on local mungbean germplasm are important for supporting breeding program. A total of 98 local mungbean varieties or accessions were evaluated at Cikeumeuh Experimental Farm,Bogor, during wet season of 2005. The experiment was conducted in a randomized block design with three replications. Each variety was planted in three rows of four meters long. Plant spacing was 40x20 cm, each hill contained two plants. The differences among the varieties were significant for all the characters studied, except for number of seeds per pod and pod length. High yielding varieties were recorded from Demak, Belu, Pati, and Jeneponto. These varieties had a combination of high number of pods per plant, large seed size and early maturity.Seeds weight per plant, pods per plant and seed size had high heritability and expected genetic advance.While the heritability and expected genetic advance for number of branches, pod length, and seeds per pod were all low. Plant height had a high genotypic variance associated with high heritability and high expected genetic advance. Similarly for days to flowering and days to maturity is genotypic in nature with high heritability coupled with a low expected genetic advance for days to flowering and moderate expected genetic advance for days to maturity. Pods per plant, seed size and seed weight per plant had a high genotypic variance associated with high heritability. The genetic advance of these characters predicted that the greatest gain for one generation of selection would be obtained by selection for pods per plant (45.07%), seed size (41.88%) and seed weight per plant (37.03%).

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