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Dental Journal (Majalah Kedokteran Gigi)
Published by Universitas Airlangga
ISSN : 19783728     EISSN : 24429740     DOI : -
Core Subject : Health,
Dentistry
The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.
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Articles 964 Documents
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Plaque index between blind and deaf children after dental health education Cynthia Carissa; Jakobus Runkat; Yetty Herdiyati
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 1 (2011): March 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.413 KB) | DOI: 10.20473/j.djmkg.v44.i1.p39-42

Abstract

Background: Difficulty in mobility and motor coordination could affect the health at teeth and mouth. Dental health education of the blind and deaf children differs according their limitation. Blind and deaf children need a particular guidance in dental health education to promote oral hygiene as normal children do. Purpose: The objective of this study was to observe the difference of plaque index between blind and deaf children before and after dental health education. Methods: This research used purposive sampling technique. Twenty-three blind children were taken as samples from SLB-A Negeri Bandung and 31 deaf children from SLB-B Cicendo Bandung. The data were then collected through plaque index examination using modified patient hygiene performance (PHP) test. Results: The result descriptively showed that plaque index average value of 23 blind children before dental health education was 3.0725 and after, was 1.7970. On the other hand, the plaque index average of deaf children before dental health education was 2.7474 and after was 1.5. Conclusion: It is concluded that plaque index of deaf children is better than blind children before and after dental health education.Latar belakang: Kesulitan dalam pergerakan dan koordinasi motorik akan memengaruhi kesehatan gigi dan mulut. Pendidikan kesehatan gigi dan mulut anak buta dan tuli akan berbeda tergantung tingkat kekurangan mereka. Anak tunanetra dan anak tunarungu membutuhkan pendidikan khusus berupa pendidikan kesehatan gigi untuk meningkatkan kebersihan gigi dan mulut serupa dengan anak normal. Tujuan: Untuk mengetahui perbedaan indeks plak antara anak-anak buta dan tuli sebelum dan sesudah pendidikan kesehatan gigi. Metode: Penelitian ini menggunakan teknik purposive sampling. Dua puluh tiga anak tunanetra diambil sebagai sampel dari SLB-A Negeri Bandung dan 31 anak tunarungu dari SLB-B Cicendo Bandung. Data tersebut kemudian dikumpulkan melalui pemeriksaan indeks plak menggunakan indeks patient hygiene performance (PHP) modifikasi. Hasil: Hasil penelitian secara deskriptif menunjukkan bahwa nilai indeks plak rata-rata 23 anak tunanetra sebelum pendidikan kesehatan gigi adalah 3,0725 dan sesudah pendidikan kesehatan gigi adalah 1,7970. Sedangkan, indeks plak rata-rata anak tunarungu sebelum pendidikan kesehatan gigi adalah 2,7474 dan sesudah pendidikan kesehatan gigi adalah 1,5. Kesimpulan: Indeks plak anak tunarungu lebih baik dibandingkan dengan anak tunanetra sebelum dan sesudah pendidikan kesehatan gigi.
DNA Epstein-Barr virus (EBV) sebagai biomaker diagnosis karsinoma nasofaring Janti Sudiono; Irma Hassan
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 3 (2013): September 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.289 KB) | DOI: 10.20473/j.djmkg.v46.i3.p140-147

Abstract

Background: Nasopharyngeal carcinoma (NPC) is a malignant neoplasm arising from the mucosal epithelium of the nasopharynx with various cells differentiation. Nasopharyngeal carcinoma is vastly more common in certain regions of East Asia, South Asia and Africa with viral, dietary which is typically includes consumption of salted vegetables, fish, meat and genetic factors that implicated in its causation. The undifferentiated is the most common type of NPC and strongly associated with Epstein-Barr virus (EBV) infection. Purpose: This paper was aimed to review about molecular biomarker as non invasive diagnosis of NPC especially in related to EBV infection in nasopharyngeal epithelial cells. Reviews: The pathogenesis of NPC particularly the endemic type seems to follow a multi-step process, in which EBV, ethnic background, and environmental carcinogens all seem to play important role. EBV DNA plasm level is used continuously in clinic as a promise, sensitive and specific molecular marker diagnostic that reflected the stage, treatment response and prognosis of NPC. Detection of nuclear antigen associated with Epstein-Barr virus (EBNA) and viral DNA has revealed that EBV can infect epithelial cells and associated with their transformation in carcinogenesis. Latent membrane protein (LMP-1 and LMP-2) oncogenes EBV encoded related to proliferative gene expression indicated invasive and progressive growth of NPC. Conclusion: The new biomarkers for NPC, including EBV DNA in serum; EBV DNA and BamH1-A Reading Frame-1 (BARF1) mRNA in NPC brushings have been developed for the molecular non invasive diagnosis of this tumour.Latar belakang: Nasopharyngeal carcinoma (NPC), sering dikenal sebagai kanker nasofaring merupakan tumor ganas yang berasal dari epitel mukosa nasofaring dengan derajat diferensiasi sel yang bervariasi. Paling banyak ditemukan di Asia Selatan, Asia Timur, dan Afrika. Virus, pola diet tipikal seperti konsumsi sayuran, ikan dan daging yang diasinkan, dan faktor genetik merupakan faktor kausatif. Tipe undifferentiated paling banyak ditemukan dan sangat berkaitan dengan infeksi virus Epstein Barr (EBV). Tujuan: Tujuan penulisan ini akan meninjau pustaka mengenai biomarker molekular sebagai alat diagnostik yang non invasif untuk NPC terutama dalam kaitannya dengan infeksi EBV pada sel epitel nasofaring. Tinjauan pustaka: Patogenesis NPC terutama pada tipe endemik, merupakan proses multi tahap, dan semua faktor seperti EBV, latar belakang etnik, dan karsinogen lingkungan berperan penting. Level plasma DNA EBV digunakan secara rutin di klinik sebagai suatu marker diagnostik molekular yang menjanjikan, sensitif, dan spesifik sebagai cerminan stadium, respon terhadap pengobatan dan prognosis NPC. Terdeteksinya antigen inti yang berkaitan dengan EBV (EBNA) dan DNA virus menyatakan bahwa EBV menginfeksi sel epitel dan terkait dengan transformasi sel dalam karsinogenesis. Protein membran laten-1 dan 2 onkogen (LMP1 dan LMP2) mengkode EBV berkaitan dengan ekspresi gen pertumbuhan sel yang mengindikasikan pertumbuhan yang sangat invasif dan progesif dari NPC. Simpulan: Biomarker NPC terkini seperti pengukuran EBV DNA dalam serum; EBV DNA dan BARF1 (BamH1-A Reading Frame-1) mRNA pada sitologi NPC telah dikembangkan untuk diagnosis molekular yang non invasif
Tensile bond strength of hydroxyethyl methacrylate dentin bonding agent on dentin surface at various drying techniques Kun Ismiyatin
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 2 (2010): June 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.22 KB) | DOI: 10.20473/j.djmkg.v43.i2.p54-57

Abstract

Background: There are several dentin surface drying techniques to provide a perfect resin penetration on dentin. There are two techniques which will be compared in this study. The first technique was by rubbing dentin surface gently using cotton pellet twice, this technique is called blot dry technique. The second technique is by air blowing dentin surface for one second and continued by rubbing dentin surface gently using moist cotton. Purpose: This experiment was aimed to examine the best dentin surface drying techniques after 37% phosphoric acid etching to obtain the optimum tensile bond strength between hydroxyethyl methacrylate (HEMA) and dentin surface. Method: Bovine teeth was prepared flat to obtain the dentin surface and than was etched using 37% phosphoric acid for 15 seconds. After etching the dentin was cleaned using 20 cc plain water and dried with blot dry techniques (group I), or dried with air blow for one second (group II), or dried with air blow for one second, and continued with rubbing gently using moist cotton pellet (group III), and without any drying as control group (group IV). After these drying, the dentin surfaces were applied with resin dentin bonding agent and put into plunger facing the composite mould. The antagonist plunger was filled with composite resin. After 24 hours, therefore bond strength was measured using Autograph. Result: Data obtained was analyzed using One-Way ANOVA with 95% confidence level and continued with LSD test on p≤0.05. The result showed that the highest tensile bond strength was on group I, while the lowest on group IV. Group II and IV, III and IV, II and III did not show signigicant difference (p>0.05). Conclusion: Dentin surface drying techniques through gentle rubbing using cotton pellet twice (blot dry technique) gave the greatest tensile bond strength.Latar belakang masalah: Tehnik pengeringan permukaan dentin agar resin dapat penetrasi dengan sempurna adalah dengan cara pengusapan secara halus sebanyak dua kali menggunakan bulatan kapas, yang disebut blot dry technique, dengan semprotan udara selama 1 detik atau dengan semprotan udara selama 1 detik yang dilanjutkan dengan pengusapan secara halus menggunakan bulatan kapas basah yang diperas dan kelebihan air pada kapas diserap dengan kertas hisap. Tujuan: Penelitian ini bertujuan untuk mengetahui tehnik pengeringan permukaan dentin yang terbaik setelah etsa dengan asam phosphat 37% untuk mendapatkan kekuatan perlekatan tarik yang optimum antara HEMA dentine bonding agent dan permukaan dentin dengan menggunakan alat ukur Autograph. Metode: Permukaan dentin gigi sapi diasah rata, kemudian di etsa dengan asam phosphat 37% selama 15 detik. Permukaan dentin dicuci dengan 20 cc air dan kemudian dikeringkan dengan cara blot dry technique (kel. I), dengan semprotan udara selama 1 detik (kel. II) atau semprotan udara selama 1 detik dan dilanjutkan dengan pengusapan secara halus menggunakan bulatan kapas basah yang diperas dan kelebihan air pada kapas diserap dengan kertas hisap (kel. III) dan tanpa pengeringan permukaan dentin sebagai kontrol (kel. IV). Selanjutnya permukaan dentin diulasi dengan resin bonding dan diletakkan kedalam plunger dengan permukaan menghadap permukaan komposit. Setelah 24 jam dilakukan pengukuran kekuatan tarik dengan menggunakan alat ukur Autograph. Hasil: Analisis data menggunakan uji Anova satu arah dengan derajat kepercayaan 95% dan dilanjutkan dengan test LSD pada p≤0,05. Dari hasil penelitian didapatkan kekuatan perlekatan tarik yang paling tinggi pada kelompok I, sedangkan yang paling rendah pada kelompok IV. Didapatkan perbedaan bermakna pada kelompok I, II, III, dan IV. Pada kelompok II dan IV, III, dan IV, II, dan III tidak didapatkan perbedaan bermakna (p>0,05). Kesimpulan: Tehnik pengeringan permukaan dentin dengan cara pengusapan secara halus menggunakan bulatan kapas sebanyak 2 kali menghasilkan kekuatan perlekatan tarik terbesar.
Identifikasi bite marks dengan ekstraksi DNA metode Chelex (Bite marks identification with Chelex methods in DNA extraction) Imelda Kristina Sutrisno; Ira Arundina; Agung Sosiawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 2 (2013): June 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (460.296 KB) | DOI: 10.20473/j.djmkg.v46.i2.p107-112

Abstract

Background: In the case of crime often encountered evidence in bite marks form that was found on the victim’s body. Generally, bitemarks identification use standard techniques that compare the interpretation picture with the tooth model of suspected person. However, sometimes the techniques do not obtain accurate results. Therefore another technique is needed to support the identification process,such as DNA analysis that use the remaining epithelium attached in saliva to identify the DNA of the suspected person. In this processes a limited DNA material could be met, not only less in quantity but also less in quality. Chelex known as one of an effective DNA extraction method in DNA forensic case is needed to overcome this problem. Purpose: The study was aimed to examine the use of Chelex as DNA extraction method on a bitemarks sample models. Methods: The blood and bitemarks of 5 persons with were taken. The DNA of each subject was exctracted with Chelex and quantified the quantity with UV Spechtrophotometer. The DNA results was amplified by PCR at locus vWA and TH01 then vizualised by electrophoresis. Results: The electrophoresis’s results showed band at locus vWA and TH01 for blood sample and bite marks with no significant differences. Conclusion: The study showed that Chelex method could be use to extract DNA from bitemarks.Latar belakang: Dalam kasus kejahatan sering dijumpai bukti dalam bentuk bekas gigitan (bitemarks) yang ditemukan pada tubuh korban. Umumnya, untuk mengidentifikasi bite marks menggunakan teknik standar yaitu membandingkan foto interpretasi dengan model gigi dari orang yang dicurigai. Namun demikian teknik ini terkadang tidak mendapatkan hasil yang akurat, sehingga diperlukan teknik lain untuk menunjang keberhasilan proses identifikasi pelaku, yakni melalui analisis DNA bitemarks, yang diperoleh dari saliva yang mengandung sisa epitel tersangka pelaku. Sampel DNA yang berasal dari bitemarks umumnya terbatas, tidak hanya terbatas dalam kuantitas tetapi juga terbatas dalam kualitas. Hal ini seringkali menimbulkan kesulitan tersendiri dalam proses analisisnya. Chelex yang dikenal sebagai salah satu metode ekstraksi yang efektif di bidang forensik, sangat diperlukan untuk mengatasi kendala tersebut . Tujuan: Penelitian ini bertujuan untuk meneliti penggunaan metode ekstraksi DNA metode Chelex pada sampel bite marks. Metode: Darah dan cetakan gigi dari 5 subjek diambil, dan DNA di ekstraks dengan Chelex dan kemudian diuji kuantitas dengan UV Spechtrophotometer. Setelah itu hasil diamplifikasi dengan PCR pada lokus vWA dan TH01 kemudian divisualisasi dengan elektroforesis. Hasil: Hasil elektroforesis menunjukkan adanya band pada lokus vWA dan TH01 untuk sampel darah dan cetakan gigi tanpa perbedaan yang signifikan secara statistika. Simpulan: Penelitian ini menunjukkan bahwa metode Chelex dapat digunakan untuk mengekstraksi DNA dari bite marks.
The combination of miacalcic, calcium lactate, and vitamin C as postextracted alveolar bone resorption inhibitor Sri Kentjananingsih
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 3 (2010): September 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.582 KB) | DOI: 10.20473/j.djmkg.v43.i3.p141-145

Abstract

Background: Tooth extraction can cause alveolar resorption, and will reduce the denture retention. The process of bone resorption looks like the process of osteoporosis. Calcium and vitamin D supplementation is the rational therapy for minimizing bone loss. Miacalcic is the drug of choice for osteporotic patient. Purpose: This study is aimed to know whether the combination of miacalcic, calcium lactate, and vitamin C are effective in inhibiting post extracted alveolar resorption. Methods: Thirty three healthy postmenopausal women were chosen as samples and they were classified randomly into control group (without treatment), 1st experiment group (treatment was started 3 months post extraction), and 2nd experiment group (treatment was started at the 2nd day post extraction). The treatment was done by giving miacalcic nasal spray, calcium lactate 500 mg and vitamin C 100 mg tablets every morning in 10 days every month for 3 months. X-ray photo of the post extracted area were taken an hour, 3 months, and 6 months post-extraction. Results: After 6 month, there was significant difference in buccolingual thickness decreasing among three groups (p<0.05). The maximum mean difference of buccolingual thickness decreasing was 0.72 mm, between control and 2nd experiment groups. There was no significant difference about decreasing bone density among them (p>0.10). The maximum difference of the mean of density decreasing was 1,906 g/cm2/mm between control and 2nd experiment groups. The increasing density mostly occurred in the 2nd experiment group. Conclusion: The combination of miacalcic, calcium lactate, and vitamin C are effective for inhibiting alveolar resorption, although statistically there was no significant difference about bone density decreasing. The sooner this treatment is given the better result will be achieved.Latar belakang: Pencabutan gigi menyebabkan resorpsi tulang alveolaris, dan akan mengurangi retensi geligi tiruan. Proses resorpsi tulang alveol pada osteoporosis mirip dengan proses resorpsi tulang pada penyembuhan luka bekas pencabutan. Miacalcic adalah obat utama untuk penderita osteoporosis. Kalsium dan vitamin D merupakan terapi yang rasional untuk meminimalkan resorpsi tulang. tujuan: Membuktikan apakah kombinasi miacalcic, kalsium laktat, and vitamin C juga efektif menghambat resorpsi tulang alveol pasca pencabutan. Metode: Sampel 33 wanita postmenopause yang sehat, terbagi secara acak ke dalam kelompok kontrol (tanpa perlakuan), kelompok eksperimen 1 (perlakuan mulai 3 bulan pasca pencabutan) dan kelompok eksperimen 2 (perlakuan mulai hari kedua pasca pencabutan). Perlakuannya yaitu: pemberian miacalcic semprot hidung, tablet kalsium laktat 500 mg dan vitamin C 100 mg setiap pagi, 10 hari dalam sebulan, selama tiga bulan. Foto sinar-X dari regio pasca pencabutan dibuat satu jam, 3 bulan, dan 6 bulan pasca pencabutan. Hasil: 6 bulan pasca-cabut, ada beda bermakna perihal selisih tebal bukolingual tulang alveol antar ketiga kelompok (p<0,05). Rerata penurunan ketebalan ini maksimal sebanyak 0.72 mm, antara kelompok kontrol dan kelompok eksperimen 2. Penurunan kepadatan tulang antar ketiga kelompok tidak bermakna (p>0,10). Beda maksimum rerata kepadatan tulang antara kelompok kontrol dan kelompok eksperimen 2 sebesar 1,906 g/cm2/mm. Peningkatan kepadatan terbanyak dialami anggota kelompok eksperimen 2. Kesimpulan: Kombinasi miacalcic, kalsium laktat, vitamin C efektif menghambat resorpsi tulang alveolaris, walaupun secara statistik beda penurunan kepadatan tidak bermakna. Makin awal pemberian perlakuan, hasilnya akan lebih baik.
The effect of nickel as a nickel chromium restoration corrosion product on gingival fibroblast through analysis of BCl-2 FX Ady Soesetijo; Mandojo Rukmo
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 4 (2012): December 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (846.239 KB) | DOI: 10.20473/j.djmkg.v45.i4.p202-207

Abstract

Background: Restoration of NiCr may undergo corrosion process in artificial saliva. Corrosion product is soluble Ni substances in salivary electrolytes. Ni2+ may freely enter the cells through passive transport DMT-1. Ni2+ in the cell causes initiation of the ROS formation,which subsequently can conduct the redoxs reactions leading to DNA damage. The damage DNA affects the genetic expression, especially bcl-2, and even triggers apoptosis. Purpose: The aim of this study was to reveal the mechanism of Ni toxicity as a corrosion product of NiCr restoration on gingival fibroblasts through expression analysis of Bcl-2. Methods: Cells with a density of 105 planted on each coverslip in 72 wells to the treatment group and 24 wells to the control group (24 hours incubation). In the treatment groups, each well exposed with 20 μL artificial saliva containing Ni concentration results immerse each restoration, whereas the control group was exposed to 20 μL artificial saliva (incubation 1, 3, and 7 days). The data collected were subsequently analyzed using two-ways ANOVA, followed by one-way ANOVA. Comparing between experimental groups after one-way ANOVA was conducted using Fisher’s LSD. Whereas, the calculation and documentation of Bcl-2 expression was performed camera of Olympus Microscope BX-50 Japan. Results: Statistical analysis of two-ways ANOVA showed the presence of interaction between the increasing Ni concentration and exposure duration on the expression of Bcl-2 gingival fibroblasts (p=0.021<a=0.05). Conclusion: It can be concluded that the higher concentration of Ni exposed to gingival fibroblasts, and the longer incubation time will decreased Bcl-2 expression.Latar belakang: Restorasi NiCr dapat mengalami proses korosi di dalam saliva artificial. Produk korosi yang dihasilkan adalah substansi Ni yang terlarut di dalam elektrolit saliva. Ni2+ bebas dapat memasuki sel (fibroblas gingiva) melalui transport pasif DMT-1. Ni2+ di dalam sel menginisiasi pembentukan ROS, yang selanjutnya dapat menjalankan reaksi redoks dan dapat menimbulkan kerusakan DNA. DNA yang rusak mempengaruhi ekspresi genetik, terutama Bcl-2 dan bahkan dapat memicu apoptosis. Tujuan: Tujuan penelitian ini adalah untuk mengungkap mekanisme toksisitas Ni sebagai suatu produk korosi restorasi NiCr pada fibroblas gingiva melalui analisis ekspresi Bcl-2. Metode: Sel dengan kepadatan 105 ditanam pada tiap-tiap coverslip di dalam 72 well untuk kelompok perlakuan dan ditanam pada tiap-tiap coverslip di dalam 24 well untuk kelompok kontrol (inkubasi selama 24 jam). Pada kelompok perlakuan, masing-masing well dipapar dengan 20 μL saliva artificial yang mengandung konsentrasi Ni hasil perendaman tiap-tiap restorasi, sedangkan pada kelompok kontrol dipapar 20 μL saliva artificial (inkubasi 1,3 dan 7 hari). Data yang terkumpul selanjutnya dianalisis menggunakan ANOVA dua arah dan ANOVA satu arah. Perbandingan antar kelompok eksperimental setelah analisis ANOVA satu arah menggunakan uji Fisher’s LSD. Penghitungan jumlah sel yang mengekspresikan Bcl-2, kemudian dilanjutkan dengan dokumentasi dengan menggunakan kamera Olympus Microscope BX-50 Japan. Hasil: Analisis statistik ANOVA dua arah menunjukkan adanya interaksi antara peningkatan konsentrasi Ni dan lama paparan terhadap ekspresi Bcl-2 fibroblas gingiva > (p = 0,021 < á = 0,05). Kesimpulan: Dapat disimpulkan bahwa semakin tinggi konsentrasi paparan Ni pada fibroblas gingiva dan semakin lama masa inkubasi, maka akan menurunkan ekspresi Bcl-2.
Facial reconstruction using polypropylene mesh after resection of maxillary ossifying fibroma R. Soesanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 3 (2012): September 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1296.363 KB) | DOI: 10.20473/j.djmkg.v45.i3.p172-176

Abstract

Background: Ossifying fibroma is a variant of fibrous dysplasia and catagorized as osteofibrosis lesion. It commonly affects long bones but occasionally involves jaws. Mandible affected more common than maxilla. The treatment of ossifying fibroma include excision and resection. Excision of lesion and resection of maxilla could cause facial defect which cannot be reconstructed with bone graft. Purpose: The aim of this case report is to report the potential use of polypropylene mesh in facial reconstruction after hemimaxillectomy in patients diagnosed with ossifying fibroma of the maxilla. Case: A 17-years-old female patient came to Oral and Maxillofacial Surgery clinic, Dental Hospital, Faculty of Dentistry, Airlangga University, with chief complaint of swelling in the upper left cheek of 2 years duration which was not related to any history of toothache. Patient also complained of the itchiness and pain on the swelling area occasionally as well as salty discharge from the mass. The FNA was done and the citology result indicated a benign mesenchimal tumor. Incisonal biopsy was subsequently performed and the histopathology report confirmed the diagnosis of cementifying fibroma. Case management: Left hemimaxillectomy was done, and post surgical defect in the facial and buccal aspect was immediately reconstructed using three layers of polypropylene mesh. On follow-up, eleven months post-operatively, the patient was well and there was no facial deformity or asymmetry. Conclusion: Polypropylene mesh is a potential material for facial reconstruction as it can reduce the risk of facial deformity after hemimaxillectomy of patients with tumor of the maxilla.Latar belakang: Ossifying fibroma merupakan varian dari fibrous dysplasia dan termasuk di dalam kategori lesi osteofibrosis. Umumnya mengenai tulang panjang tetapi dapat juga mengenai rahang dan lebih banyak menyerang tulang mandibula dibanding maksila. Perawatan ossifying fibroma dilakukan dengan eksisi atau reseksi. Perawatan eksisi atau reseksi tulang maksila dapat mengakibatkan deformitas wajah yang tidak dapat direkontruksi dengan bone graft. Tujuan: Laporan kasus ini bertujuan untuk melaporkan penggunaan polipropylene mesh pada rekonstruksi wajah setelah hemimaxillectomy pada pasien dengan diagnose ossifying fibroma pada maksila. Kasus: Penderita wanita berusia 17 tahun datang ke Klinik Bedah Mulut dan Maksilofasial, Rumah Sakit Gigi dan Mulut Fakultas Kedokteran Gigi Universitas Airlangga, dengan keluhan utama pembengkakan pada pipi kiri atas yang muncul sejak 2 tahun yang lalu tanpa ada riwayat keluhan sakit gigi. Penderita mengeluh gatal, kadang sakit dan keluarnya cairan asin pada daerah benjolan. Hasil FNA dan test sitologi menunjukkan gambaran tumor jinak mesensimal. Pemeriksaan dengan biopsi dan histopatologi mengkonfirmasi diagnosa cementifying fibroma. Tatalaksana kasus: Dilakukan hemimaksilektomy pada sebelah kiri, dan defek pasca pembedahan pada sisi fasial dan bukal segera direkontruksi dengan pemasangan polipropylene mesh 3 lapis. Sebelas bulan setelah operasi kondisi pasien baik dan tidak ada deformitas atau asimetri wajah. Kesimpulan: Polypropylene mesh merupakan bahan yang potensial untuk rekonstruksi wajah karena dapat mengurangi terjadinya deformitas wajah setelah hemimaxillectomy pada pasien dengan tumor maksila.
The decreasing of NFκB level in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with application of 1% curcumin on gingival sulcus Agung Krismariono
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 1 (2015): March 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.448 KB) | DOI: 10.20473/j.djmkg.v48.i1.p35-38

Abstract

Background: Periodontal disease is a chronic, multi-factorial disease. Chronic periodontitis is one of the main causes of tooth loss. Chronic periodontitis is usually caused by Porphyromonas gingivalis (P. gingivalis). P. gingivalis can induce NFκB activation resulting in the increasing of periodontal extracellular matrix degradation. Curcumin can inhibit NFκB activation and reduce the severity of periodontal degradation. Purpose: This research was aimed to observe level of NFκB in gingival junctional epithelium of rat exposed to Porphyromonas gingivalis with local administration of curcumin. Methods: Sixteen Wistar rat were divided into two groups. Group 1 (treatment) consisted of eight rat given 2 x 106CFU/ml P. gingivalis and 1% curcumin. Meanwhile, group 2 (control) consisted of eight rat given 2 x 106 CFU/ml P. gingivalis only. GCF samples were collected from gingival sulcus. The samples were biochemically analyzed with ELISA method. Data were then analyzed statistically by using independent t-test (α=0.05). Results: The examination of NFκB level showed that there was significant difference between treatment group and control group (p<0.05). The level of NFκB in the treatment group was significantly lower than the control group. Conclusion: It can be concluded that 1% curcumin application can reduce NFκB level in gingival junctional epithelium of rat exposed to P. gingivalis.
Degrees of chitosan deacetylation from white shrimp shell waste as dental biomaterials Sularsih Sularsih; Anita Yuliati; Coen Pramono D
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 1 (2012): March 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (318.046 KB) | DOI: 10.20473/j.djmkg.v45.i1.p17-21

Abstract

Background: Chitosan is biomaterial improved for various dentistry applications because it is biocompatible, degradable, nontoxic, and not carcinogenic. The main parameter affecting the characteristics of chitosan is deacetylation degree. Purpose: This study is aimed to determine the degree of deacetylated of chitosan derived from white shrimp shell waste used as dental biomaterial. Methods: White shrimp shells were crushed into powder. Next, deproteination process was conducted with 3.5% NaOH solution, demineralized with 1N HCl solution, and then depigmented with 90% acetone solution into chitin powder. Deacetylation process was then conducted by soaking the chitin powder in 50% NaOH solution for 6 h at 65° C to produce white powder of chitosan. Afterwards, deacetylation degree test was conducted by using Fourier Transform Infrared Spectrophotometer (FTIR) to calculate the ratio of the absorption bands between the absorbance peak of amide group about 1655 cm–1 and the absorbance peak of hydroxyl group about 3450 cm–1. Results: The result of the deacetylation degree test on the chitosan powder derived from white shrimp shell waste was high, about 85.165%, and had the eligible form, solubility, and pH. Conclusion: It can be concluded that the deacetylation degree of chitosan from white shrimp shells could reach 85.165%.Latar belakang: Kitosan merupakan biomaterial yang dikembangkan untuk berbagai aplikasi kedokteran gigi karena biokompatibel, dapat didegradasi, tidak toksik dan tidak karsinogenik. Parameter utama yang mempengaruhi karakteristik kitosan adalah derajat deasetilasi. Tujuan: Tujuan dari penelitian ini adalah mengetahui derajat deasetilasi kitosan dari limbah kulit udang putih sebagai biomaterial kedokteran gigi. Metode: Kulit udang putih dihaluskan menjadi serbuk. Setelah itu dilakukan proses deproteinasi dengan larutan NaOH 3,5%, demineralisasi dengan larutan HCl 1N, depigmentasi dengan larutan aseton 90% sehingga menjadi serbuk kitin. Proses deasetilasi dilakukan dengan merendam serbuk kitin dalam larutan NaOH 50% selama 6 jam pada suhu 65° C sehingga dihasilkan serbuk putih kitosan. Uji derajat deasetilisasi menggunakan metode spektrofotometer Fourier Transform Inframerah (FTIR) dengan menghitung nilai perbandingan pita serapan antara puncak absorbansi gugus amida sekitar 1655 cm–1, dan puncak absorbansi gugus hidroksil sekitar 3450 cm–1. Hasil: Hasil uji derajat deasetilasi serbuk kitosan dari limbah kulit udang putih adalah tinggi yaitu sebesar 85.165% dan memiliki bentuk, kelarutan dan pH yang memenuhi syarat. Kesimpulan: Dapat disimpulkan derajat deasitilasi kitosan dari kulit udang putih adalah 85,165%.
In vitro effect of Q-switched Nd:YAG laser exposure on morphology, hydroxyapatite composition and microhardness properties of human dentin Retna Apsari; Siswanto Siswanto; Anita Yuliati; Noriah Bidin
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 4 (2011): December 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.266 KB) | DOI: 10.20473/j.djmkg.v44.i4.p181-186

Abstract

Background: A Q-switched Nd:YAG laser was employed as a source of ablation. The fundamental wavelength of the laser is 1064 nm, with pulse duration of 8 nanosecond operates with uniphase mode of TEM00. In the following experiments, dentin samples (without caries and plaque) are exposed to pulse laser with Q-switching effect at various energy dose. Purpose: The aim of this study was to investigate the effect of laser ablation on dentin samples using Q-switched Nd:YAG laser exposure. Methods: The laser was operated in repetitive mode with frequency of 10 Hz. The energy dose of the laser was ranging from 13.9 J/cm2, 21.2 J/cm2 and 41.7 J/cm2. The target material comprised of human dentin. The laser was exposed in one mode with Q-switched Nd:YAG laser. Energy delivered to the target through free beam technique. The exposed human dentin was examined by using x-ray diffraction (XRD) and fluoresence scanning electron microscopy for energy dispersive (FESEM-EDAX). Microhardness of human dentin were examined by using microhardness vickers test (MVT). Results: The result obtained showed that the composition of hydroxyapatite of the dentin after exposed by Q-switched Nd:YAG laser are 75.02% to 78.21%, with microhardness of 38.7 kgf/mm2 to 86.6 kgf/mm2. This indicated that exposed pulsed Nd:YAG laser on the human dentin attributed to the phototermal effect. The power density created by the Q-switched Nd:YAG laser enables the heat to produce optical breakdown (melting and hole) associated with plasma formation and shock wave propagation, from energy dose of 21.2 J/cm2. From XRD analysis showed that the exposure of Nd:YAG laser did not involve in changing the crystal structure of the dentin, but due to photoablation effect. Conclusion: In conclusion, the application of Q-switched Nd:YAG laser as contactless drills in dentistry should be regarded as an alternative to the classical mechanical technique to improve the quality of the dentin treatment.Latar belakang: Mode Q-Switch pada laser Nd:YAG dapat menghasilkan fenomena ablasi pada dentin. Laser Nd:YAG yang digunakan mempunyai panjang gelombang 1064 nm, durasi pulsa 8 ns beroperasi dengan mode TEM00. Sampel dentin yang digunakan tanpa karies dan plak, yang dipapari laser dengan mode Q-switch dalam berbagai variasi dosis energi. Tujuan: Tujuan penelitian ini adalah mengamati efek ablasi dentin secara in vitro akibat paparan laser Nd:YAG Q-switch dengan pengamatan morfologi permukaan, komposisi hidroksiapatit, dan uji kekerasan mikro. Metode: Laser Nd:YAG Q-switch dengan frekuensi 10 Hz dan variasi dosis energi 13,9 J/cm2, 21,2 J/cm2 dan 41,7 J/cm2 ditembakkan pada sampel dentin manusia dengan teknik penyinaran bebas tanpa dilewatkan serat optik. Dentin yang terbuka diamati menggunakan x-ray diffraction (XRD) dan fluoresence scanning electron microscopy for energy dispersive (FESEM-EDAX). Kekerasan mikro dari dentin juga diamati menggunakan microhardness vickers test (MVT). Hasil: Hasil penelitian menunjukkan bahwa komposisi hidroksiapatit dari dentin setelah paparan laser Nd:YAG Q-Switch menunjukkan peningkatan berkisar 75,02% sampai 78,21% dibandingkan normal, dengan kekerasan mikro berkisar 38,7 kgf/mm2 sampai 86,6 kgf/mm2. Perubahan pada struktur mikro tersebut disebakan karena adanya efek fototermal. Kerapatan daya yang bervariasi berdasarkan variasi dosis energi menyebabkan efek panas pada dentin yang menyebabkan adanya fenomena optical breakdown, yang ditandai dengan munculnya efek leleh dan lubang pada sampel karena produksi plasma dan adanya gelombang kejut, mulai dosis 21,2 J/cm2. Berdasarkan uji XRD, efek yang muncul pada dentin tidak menyebabkan terjadinya perubahan struktur kristal hidroksiapatit, tetapi menyebabkan perubahan komposisi hidroksiapatit yang disebut dengan fotoablasi. Kesimpulan: Dapat disimpulkan bahwa penggunaan mode Q-switched pada laser Nd:YAG sebagai alat dengan kontak minimal dapat dijadikan teknik alternatif untuk meningkatkan kualitas perawatan dental.

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