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All Journal Majalah Kedokteran Gigi Indonesia Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Dentomaxillofacial Science Jurnal Pengabdian UNDIKMA Strada Jurnal Ilmiah Kesehatan (SJIK)

Ira Arundina

Departemen Biologi Oral, Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya, Jawa Timur, Indonesia

Author-ID : 3196264

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Identifikasi Kromatografi Lapis Tipis Sudamala (Artemisia vulgaris L.) Ira Arundina; Theresia Indah Budhy S.; Muhammad Luthfi; Retno Indrawati
Majalah Kedokteran Gigi Indonesia Vol 1, No 2 (2015): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Identification of Sudamala (Artemisia vulgaris L.) Thin Layer Chromatography. Oral squamous cell carcinoma is the type of cancer which is most frequently found in oral cavity. The primary risk factor of malignancy in oral cavity includes the habit of consuming tobacco and or alcohol. The plant sudamala (Artemisia vulgaris L.) is often used in the community as anti-tumor in digestive organ, including in oral cavity. However, there have been no studies on active ingredients playing the role as anti-cancer in oral cavity. The species are mostly from the genus Artemisia, while those generally growing in Indonesia are the species Artemisia vulgaris L. The objective of this study is to explain the identification by TLC of sudamala. The study was sudamala extraction, identification of sudamala extract, sudamala fractionation with Vacuum Column Chromatography and identification of sudamala fractionation with TLC (Thin Layer Chromatography). The result shows that the fractionation using hexane extract resulted in 11 fractions of n-hexane: ethyl acetate. The conclusion of this study is that the fraction of n-hexane: ethyl acetate (3:7, v/v) sudamala (Artemisia vulgaris L.) identified contains terpenoids compounds.

Korelasi Jumlah Streptococcus mutans (S. mutans) dan Level Ekspresi Interlukin 8 (IL-8) pada Severe Early Childhood Caries Muhammad Luthfi; Retno Indrawati; Ira Arundina; Yoes Prijatna Dachlan
Majalah Kedokteran Gigi Indonesia Vol 1, No 2 (2015): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Correlation of Streptococcus mutans (S. mutans) Level and Interleukin 8 (IL-8) Expressions of Salivary Neutrophils in Severe Early Childhood Caries. Early childhood caries is a very serious health problem because it is a chronic infectious disease that is contagious. Dental caries begins after the primary teeth grow and develop on the tooth surface very quickly and progressively. In recent years the views of neutrophils have changed dramatically. Neutrophils not only act as a microbe killer through phagocytosis, the release of reactive oxigen species (ROS) and its antimicrobial peptide, but neutrophil activation also helps regulate the immune response. To analyze the relationship between the amount S. mutans and IL-8 expression of salivary neutrophils in severe early Childhood caries. Two groups, namely Isolation of S. mutans were performed on saliva samples taken from 20 caries-free and 20 severe early childhood caries and samples Nacl 1,5% mouthwash results of 20 caries-free and 20 severe early childhood caries salivary neutrophils that were analysis of IL-8 expression by flow cytometry. Based on the average value, it is known that S. mutans level in early Childhood caries-free is lower (513.500,00 +185.565,28 CFU/ml) in comparison to the severe early Childhood caries (977.000,00 +222.500,15 CFU/ml), but the expression of IL-8 neutrophil salivary neutrophils in early Childhood caries-free is higher (3,31+0,50) in comparison to the severe early Childhood caries (2,95+0,56). The increased S. mutans level is probably caused by the decrease in the expression of IL-8 salivary neutrophils in severe early childhood caries.

Identifikasi bite marks dengan ekstraksi DNA metode Chelex (Bite marks identification with Chelex methods in DNA extraction) Imelda Kristina Sutrisno; Ira Arundina; Agung Sosiawan
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 2 (2013): June 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: In the case of crime often encountered evidence in bite marks form that was found on the victim’s body. Generally, bitemarks identification use standard techniques that compare the interpretation picture with the tooth model of suspected person. However, sometimes the techniques do not obtain accurate results. Therefore another technique is needed to support the identification process,such as DNA analysis that use the remaining epithelium attached in saliva to identify the DNA of the suspected person. In this processes a limited DNA material could be met, not only less in quantity but also less in quality. Chelex known as one of an effective DNA extraction method in DNA forensic case is needed to overcome this problem. Purpose: The study was aimed to examine the use of Chelex as DNA extraction method on a bitemarks sample models. Methods: The blood and bitemarks of 5 persons with were taken. The DNA of each subject was exctracted with Chelex and quantified the quantity with UV Spechtrophotometer. The DNA results was amplified by PCR at locus vWA and TH01 then vizualised by electrophoresis. Results: The electrophoresis’s results showed band at locus vWA and TH01 for blood sample and bite marks with no significant differences. Conclusion: The study showed that Chelex method could be use to extract DNA from bitemarks.Latar belakang: Dalam kasus kejahatan sering dijumpai bukti dalam bentuk bekas gigitan (bitemarks) yang ditemukan pada tubuh korban. Umumnya, untuk mengidentifikasi bite marks menggunakan teknik standar yaitu membandingkan foto interpretasi dengan model gigi dari orang yang dicurigai. Namun demikian teknik ini terkadang tidak mendapatkan hasil yang akurat, sehingga diperlukan teknik lain untuk menunjang keberhasilan proses identifikasi pelaku, yakni melalui analisis DNA bitemarks, yang diperoleh dari saliva yang mengandung sisa epitel tersangka pelaku. Sampel DNA yang berasal dari bitemarks umumnya terbatas, tidak hanya terbatas dalam kuantitas tetapi juga terbatas dalam kualitas. Hal ini seringkali menimbulkan kesulitan tersendiri dalam proses analisisnya. Chelex yang dikenal sebagai salah satu metode ekstraksi yang efektif di bidang forensik, sangat diperlukan untuk mengatasi kendala tersebut . Tujuan: Penelitian ini bertujuan untuk meneliti penggunaan metode ekstraksi DNA metode Chelex pada sampel bite marks. Metode: Darah dan cetakan gigi dari 5 subjek diambil, dan DNA di ekstraks dengan Chelex dan kemudian diuji kuantitas dengan UV Spechtrophotometer. Setelah itu hasil diamplifikasi dengan PCR pada lokus vWA dan TH01 kemudian divisualisasi dengan elektroforesis. Hasil: Hasil elektroforesis menunjukkan adanya band pada lokus vWA dan TH01 untuk sampel darah dan cetakan gigi tanpa perbedaan yang signifikan secara statistika. Simpulan: Penelitian ini menunjukkan bahwa metode Chelex dapat digunakan untuk mengekstraksi DNA dari bite marks.

Analgesic effect of coconut shell (Cocos nucifera L) liquid smoke on mice Meircurius Dwi C.S; Tantiana Tantiana; Ira Arundina
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 3 (2012): September 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Drugs can be used to eliminate pain by inhibiting the activity of conversing arachidonic acid into prostaglandin. The chemical compositions of coconut shell are cellulose, pentosan, lignin, solvent extraction, uronat anhydrous, nitrogen, and water. One active ingredient in coconut shell is phenyl propanoid (consisting in lignin structure) and guaicol. Phenyl propanoid and guaicol are phenolic compounds that can be used as antioxidant, antiseptic, anti-inflammatory, anesthetic and analgesic. Liquid smoke of coconut shell (Cocos nucifera L) contains phenolic compound is believed able to bind a component conversing arachidonic acid into prostaglandin. Purpose: The study was aimed to examine the analgesic effect of liquid smoke of coconut shell (Cocos nucifera L). Methods: The study was a laboratory experimental research, conducted on 2-3 months old male mice (Mus musculus) with 20-30 grams of weight. There were control group and treatment groups each of which had seven mice. Control group was orally given 0.01 ml/weight (ml/gr) of distilled water, after 30 minutes 0.01 ml/weight (ml/gr) of acetic acid 0.6% was delivered via intraperitoneal injection. The treatment groups were given liquid smoke of coconut shell (Cocos nucifera L) with the concentrations of 25%, 50%, and 100% respectively. The analgesic effect was then determined by decreasing of writhing reflex on mice recorded every 5 minutes for 30 minutes. Results: There were significant differences of writhing reflexes in the treatment groups given liquid smoke of coconut shell with the concentrations of 25%, 50%, and 100%. The higher concentration of liquid smoke the higher its analgesic effect. Conclusion: Liquid smoke of coconut shell (Cocos nucifera L) has analgesic effect.Latar belakang: Salah satu mekanisme obat yang digunakan untuk menghilangkan rasa nyeri adalah menghambat aktivitas konversi asam arakhidonat menjadi prostaglandin. Komposisi kimia tempurung kelapa terdiri dari selulosa, petosan, lignin, solvent, uronat unhidrat, nitrogen dan air. Salah satu bahan aktif dalam tempurung kelapa adalah phenyl propanoid (terdapat dalam struktur lignin) dan guaiakol. Phenyl propanoid dan guaiakol adalah suatu senyawa fenol yang mempunyai sifat sebagai antioksidan, antiseptic, anti-inflamasi, anastesi dan analgesik. Liquid smoke tempurung kelapa (Cocos nucifera L)mengandung senyawa fenol yang dapat mengikat komponen dalam konversi asam arakhidonat menjadi prostaglandin. Tujuan: Studi ini bertujuan untuk meneliti efek analgesik dari liquid smoke tempurung kelapa (Cocos nucifera L). Metode: Penelitian ini adalah eksperimental laboratoris dengan jenis post test only control group design pada mencit (Mus musculus) jantan usia 2-3 bulan dengan berat badan 20-30gram. Kelompok kontrol dan perlakuan terdiri dari 7 hewan coba. Kelompok kontrol diberi aquades 0.01ml/bb (ml/gr) (po) dan setelah 30 menit diberi asam asetat 0.6% 0.01ml/bb (ml/gr) (ip). Kelompok perlakuan diberi liquid smoke tempurung kelapa (Cocos nucifera L) dengan konsentrasi 25%, 50% and 100%. Efek analgesik ditentukan dengan melihat penurunan writhing reflex (liukan atau geliat tubuh hewan coba) yang dihitung setiap 5 menit selama 30 menit. Hasil: Terdapat perbedaan yang bermakna jumlah writhing reflex pada pemberian liquid smoke tempurung kelapa konsentrasi 25%, 50% dan 100%. Semakin tinggi konsentrasi liquid smoke, semakin tinggi pula efek analgesic yang ditimbulkan. Kesimpulan: Liquid smoke tempurung kelapa mempunyai efek analgesik.

Inhibitory effect of n-hexane: ethyl acetate fraction from Artemisia vulgaris L. on cell culture of oral epithelial carcinoma Ira Arundina
Dental Journal (Majalah Kedokteran Gigi) Vol. 42 No. 1 (2009): March 2009
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Sudamala herb (Artemisia vulgaris L.) is often used in society as an anti tumor for organs of digestive system including oral cavity. Nevertheless, there are still no further scientific researches on active materials which can be used as anti carcinogen in oral cavity. Most of anti carcinogens are actually obtained from the genus Artemisia. Moreover, in Indonesia, the species of the genus Artemisia that grows the most is Artemisia vulgaris L. The problem of this research, however, is that the effect of n-hexane fraction: ethyl acetate from Artemisia vulgaris L. towards the decreasing of oncogene in oral squamous cell carcinoma is still indefinite. Purpose: The objective of this research is to explain the effect of giving n-hexane : ethyl acetate (3:7) fraction containing terpenoid from Artemisia vulgaris L. towards the decreasing of oncogene in oral epithelial carcinoma cell line. Methods: The method of this research is laboratory experimental research by using squamous cell carcinoma of oral cavity on cell culture. The inhibitory percentage test in vitro, furthermore, is taken during the analysis. The result then is analyzed by probit analysis with drawing relation curve between the inhibitory percentage and concentration. Result: The result of n-hexane : ethyl acetate (3:7) fraction containing terpenoid from Artemisia vulgaris L. has the smallest IC50, 3.902 μg/ml, less than 20 μg/ml suitable with NCI criteria; thus, it can potentially be used as anti carcinogen. Conclusion: There is the decreasing of oncogenes after being given n-hexane : ethyl acetate (3:7) fraction containing terpenoid from Artemisia vulgaris L. towards oral epithelial carcinoma cell line.

The effects of golden sea cucumber extract (Stichopus hermanii) on the number of lymphocytes during the healing process of traumatic ulcer on wistar rat’s oral mucous Ira Arundina; Yuliati Yuliati; Pratiwi Soesilawati; Dian W Damaiyanti; Dania Maharani
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 2 (2015): June 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Indonesia is a country with the world’s biggest potential and producer of sea cucumbers. Golden sea cucumber contains glicosaminoglycans, such as heparan sulphate and chondroitin sulphate, which could have a positive implication on wound healing process. This acceleration of wound healing process could be observed through the increasing of lymphocytes on ulcus traumaticus. Purpose: This study aims to analyze the effects of golden sea cucumber extract on the number of lymphocytes during the healing process of traumatic ulcer on Wistar rat’s oral mucous. Method: Golden sea cucumber extrat was made with freeze-dried method, and then gel was prepared using PEG 400 and PEG 4000 solvent. Twenty male rats with mucosal ulcus made were divided into a control group and three treatment groups with 20%, 40% and 80% golden sea cucumber extracts. All samples were euthanized on day 4 and then a preparation for histopathological examination was made to examine the number of lymphocytes. Result: The biggest number of lymphocytes was found in the treatment group with 40% golden sea cucumber extract, while the lowest one was found in the control group. The results of one way Anova test then showed a significant difference between the control group and the treatment groups. And, the results of Tukey HSD showed a significant difference between the control group and the treatment group with 40% golden sea cucumber extract. Conclusion: It can be concluded that 40% golden sea cucumber (Stichopus hermanii) extract can increase the number of lymphocytes during the healing process of traumatic ulcer on Wistar rat’s oral mucous.

The amount of macrophages and activated plasma cells on wound healing process affected by spirulina Regina Purnama Dewi Iskandar; Retno Indrawati; Ira Arundina; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 4 (2015): December 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Spirulina which grows abundantly in tropical seas have been investigated to enhance immune system. The administration of spirulina in tooth extraction sockets was expected to optimise the function of immunocompetent cells. Therefore, wound healing process would be improved. Purpose: The aim of this study was to prove that administration of spirulina could influence immune system in tooth extraction sockets. Method: There were 28 Cavia cobayas used in this study and were put in group of four. Mandibular left incisive were extracted from each of them. The basis made from mixture of polyethylene glycol (PEG) 400 and PEG 4000 was administrated into each socket in control group (TG0). In addition, spirulina 12% was administrated into group TG1, spirulina 24% was administrated into group TG2, and spirulina 48% was administrated into group TG3. All of the Cavia cobaya were decapitated and the jaws were removed in day 5 after tooth extraction. The jaws were decalcified in EDTA solution, formed into paraffin block, processed for hematoxylin and eosin (H & E) and immunohistochemistry staining afterwards. Datas were analysed statistically using Anova method. Result: There was an augmentation in the number of macrophages and activated plasma cells after spirulina application. The administration of higher concentrations of Spirulina leads to greater amount of macrophages and activated plasma cells in each groups. Conclusion: In conclusion, spirulina is able to increase the amount of macrophages and activated plasma cells which play important role in healing process.

Inhibitory effect of jengkol leaf (Pithecellobium jiringa) extract to inhibit Candida albicans biofilm Muhammad Luthfi; Ira Arundina; Nizamiar Hamni
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 3 (2016): September 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Candida albicans (C. albicans) are dimorphic fungi in oral cavity, considered not only as normal flora, but also as pathogens. C. albicans have an ability to grow biofilm, which has a thick layer of outer skin structure, called as extracellular matrix. Jengkol leaves (Pithecellobium jiringa) contain alkaloids, flavonoids, terpenoids, and lectins, which have an ability as antifungal agent Purpose: This study aimed to analyze the optimum dose of jengkol leaf extract as antibiofilm against C. albicans biofilms. Method: C. albicans were cultured on yeast peptone dextrosa (YPD) media in 96-well microtiter plate flat bottom plates. There were one control group (without treatment) and three treatment groups. The first treatment group was given jengkol leaf extract at a dose of 100 mg/ ml. The second treatment group was given jengkol leaf extract at a dose of 200 mg/ ml. And, the third treatment group was given jengkol leaf extract at a dose of 400 mg/ ml. Semi quantitative method was applied to determine C. albicans biofilmsis using Crystal Violet staining technique. The absorbance of the cells then was calculated using a spectrophotometer with a wavelength of 570 nm. Result: The mean value of optical density in the control group was 1.23. The mean value of optical density in the treatment group with a dose of 100 mg/ ml was 0.2. Meanwhile, the mean value of optical density in the treatment group with a dose of 200 mg/ ml was 0.2, and 0.21 in the treatment group with a dose of 400 mg/ ml. The results also showed that there were significant differences between the control group and all of the treatment groups (p<0.05), but there was no significant difference between the treatment groups (p>0.05). Conclusion: The optimum dose of jengkol leaf extract used as antibiofilm against C. albicans biofilms is 100 mg /ml with an inhibitory percentage of 83.7%.

Increase of collagen in diabetes-related traumatic ulcers after the application of liquid smoke coconut shell Meircurius Dwi Condro Surboyo; Ira Arundina; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 2 (2017): June 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Traumatic ulcers in patients with diabetes mellitus may experience delayed healing despite their diabetic condition being controlled. Liquid smoke coconut shell containing phenolic compounds can potentially accelerate the healing process. One healing process indicator is the increased number of fibroblasts, another being the increased amount of collagen. Purpose: This study aimed to analyze the amount of collagen in traumatic ulcers in diabetics after application of liquid smoke coconut shell. Methods: Alloxan was induced in twenty-four male Wistar rats as models of diabetes mellitus. A traumatic 10 mm ulcer was made along the labial fornix incisive inferior with a round, stainless steel blade before liquid smoke coconut shell and benzydamine hydrochloride (as the control) was administrated once a day. A biopsy of the labial fornix incisive inferior was subsequently performed after the topical application for 5 and 7 days. Histological assessment was conducted to analyze the amount of collagen by means of Masson Trichome staining. Results: Histologically, the topical application of liquid smoke coconut shell for 5 days significantly increased the amount of collagen, higher than that of benzydamine hydrochloride as the control (p=0.006) (p<0.05). Meanwhile, the topical application of liquid smoke coconut shell for 7 days made the concentration of collagen no significantly different from that of benzydamine hydrochloride as the control (p=0.156) (p>0.05). Conclusion: Liquid smoke coconut shell applied for 5 days increase the amount of collagen in traumatic ulcers in diabetic patients.

The possibility of polymorphonuclear leukocyte activation in dental socket healing by freeze-dried Aloe vera induction Pratiwi Soesilawati; Ester Arijani Rachmat; Ira Arundina; Nita Naomi
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 3 (2021): September 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i3.p124-127

Background: Research has proved that Aloe vera has anti-inflammatory, anti-bacterial, and immunomodulator properties that can accelerate the healing process. These properties could be beneficial in the tooth extraction wound-healing process. Neutrophils are the first inflammatory cells to invade and are the predominant cell markers in the wound for 24 hours after injury. Polymorphonuclear leukocytes (PMN) provide initial protection against micro-organisms, as they engulf and digest foreign bodies. Purpose: The aim of this study was to prove the effect of Aloe vera 90% gel application on the PMN count in the healing process of Cavia cobaya following tooth extraction. Methods: This laboratory experimental research was done using Post-Test Only Control Group Design. The lower incisive of twelve male Cavia cobayas were extracted then divided into four groups. Group P1 and P3 had 90% Aloe vera gel applied into the socket and sutured; group K1 and K3, received no 90% Aloe vera gel application and acted as controls. Samples from group P1 and K1 were terminated and had their mandibula taken one day after tooth extraction, and those from group P3 and K3 were terminated after three days. Tissues were then processed into histology slides, and PMN cells were counted. Results: There were significant differences in the PMN count between groups K1, P1, and K3 compared with P3 (p < 0.05). Higher PMN count was shown on the group containing Aloe vera 90% gel compared with control group, both on day one and three after tooth extraction. Conclusion: Aloe vera 90% gel can increase the PMN count in the inflammation phase of the tooth extraction wound-healing process.

Co-Authors Agung Sosiawan Anis Irmawati Damaiyanti, Dian Widya Dania Maharani Dian W Damaiyanti Ester Arijani Rachmat Hendrik Setia Budi Imelda Kristina Sutrisno Ketut Suardita, Ketut Leonita Widyana Mahardikasari Lia Aulia Rachma Meircurius Dwi C.S Mohammed Aljunaid Muhammad Luthfi Muhammad Naufal Hatta Nita Naomi Nizamiar Hamni Pratiwi Soesilawati Rahmawati, Septyana Eka Regina Purnama Dewi Iskandar Retno Indrawati Roestamadji Retno Palupi Retno Pudji Rahayu Rika D. Syarif Rima Parwati Sari, Rima Parwati Roni Handayani S.Pd. M Kes I Ketut Sudiana . Sawitri Dwi Indah Pertami Sidarningsih Sidarningsih, Sidarningsih Soesilowati, Pratiwi Surboyo, Meircurius Dwi Condro Tantiana Tantiana Theresia Indah Budhy Sulisetyawati Tuti Kusumaningsih Yoes Prijatna Dachlan Yuliati

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