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Dental Journal (Majalah Kedokteran Gigi)

MKG
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Published by Universitas Airlangga

ISSN : 19783728 EISSN : 24429740 DOI : -

Core Subject : Health,

Dentistry

The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.

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Articles 964 Documents

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Physical characteristic of brown algae (Phaeophyta) from madura strait as irreversible hydrocolloid impression material Prihartini Widiyanti; Siswanto Siswanto
Dental Journal (Majalah Kedokteran Gigi) Vol. 45 No. 3 (2012): September 2012
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Brown algae is a raw material for producing natrium alginates. One type of brown algae is Sargassum sp, a member of Phaeophyta division. Sargassum sp could be found in Madura strait Indonesia. Natrium alginate can be extracted from Sargassum sp. The demand of alginate in Indonesia is mainly fulfilled from abroad, meanwhile Sargassum sp is abundantly available. Purpose: The purpose of study were to explore the potency of brown alga Sargassum sp from Madura strait as hydrocolloid impression material and to examine its physical characteristic. Methods: The methods of research including extraction natrium alginate from Sargassum sp, synthesis of dental impression material and the test of porosity, density, viscosity, and water content of impression material which fulfilled the standard of material used in clinical application in dentistry. Results: Extraction result of Sargassum sp was natrium alginate powder with cream colour, odorless, and water soluble. The water content of natrium alginate was 21.64% and the viscosity was 0.7 cPs. The best porosity result in the sample with the addition of trinatrium phosphate 4% was 3.61%. Density value of impression material was 3 gr/cm3. Conclusion: The research suggested that brown algae Sargassum sp from Madura strait is potential as hydrocolloid impression material, due to its physical properties which close to dental impression material, but still need further research to optimize the physical characteristic.Latar belakang: Alga coklat adalah sumber bahan baku material natrium alginat. Salah satu jenis alga coklat adalah Sargassum sp yang merupakan anggota divisi Phaeophyta. Sargassum sp dapat ditemukan di Selat Madura Indonesia. Natrium alginat dapat diekstraksi dari Sargassum sp. Kebutuhan akan bahan ini di Indonesia sebagian besar dipenuhi dari impor, padahal ketersediaan Sargassum sp di Indonesia sangat melimpah. Tujuan: Penelitian ini bertujuan untuk mengeksplorasi potensi alga coklat Sargassum sp dari Selat Madura sebagai bahan cetak hidrokoloid dan meneliti karakteristik fisiknya. Metode: Tahap pertama adalah ekstraksi natrium alginat dari Sargassum sp, tahap kedua yaitu sintesis bahan cetak gigi dan menguji karakteristik bahan seperti porositas, densitas, viskositas, kadar air, bahan cetak yang memenuhi standar bahan yang digunakan dalam aplikasi klinis bidang Kedokteran Gigi. Hasil: Hasil ekstraksi berupa natrium alginat bubuk dengan warna krem, tidak berbau, dan dapat larut dalam air. Kadar air natrium alginat sebesar 21,64% dengan viskositas 0,7 cPs. Porositas terbaik dalam sampel dengan penambahan trinatrium fosfat 4% yaitu 3,61%. Nilai densitas bahan cetak 3 gr/cm3. Kesimpulan: Penelitian ini menunjukkan bahwa alga coklat Sargassum sp dari Selat Madura memiliki potensi sebagai bahan cetak hydrocolloid kedokteran gigi karena memiliki karakter fisik yang mirip dengan bahan cetak kedokteran gigi, namun masih memerlukan penelitian lebih lanjut untuk mengoptimalkan karakter fisiknya.

The influence of adhesin protein from Aggregatibacter actinomycetemcomitans on IL-8 and MMP-8 titre in aggressive periodontitis Rini Revijanti Ridwan
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 1 (2015): March 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Adhesion can actually be considered as a part of both a powerful survival mechanism and a virulence mechanism for bacterial pathogens. Bacterial adhesin is an instrument for bacteria to do invasion to host. Bacterial adhesin depends on ligand interaction as a signaling mediator that will influence invasion and increase pro and anti-inflammatory because of the influence of the receptors of innate immune response. Aggregatibacter actimycetemcomitans has fimbriae included in type IV pili containing mostly with protein weighed 6.5 kDa and at least with protein weighed 54 kDa. Purpose: The purpose of this research is to analyze the influence of the induction of adhesin protein derived from A. actinomycetemcomitans on IL-8 and MMP-8 titre of Wistar rats. Methods: Adhesin protein derived from A. actinomycetemcomitans weighed 24 kDa was induced on the maxillary first molar sulcus of Wistar rats to prove that adhesin protein could affect IL-8 and MMP-8 titre. Next, to determine its influence, Elisa technique was conducted. Results: It is known that the levels of IL-8 and MMP-8 titre were increased in the group induced with adhesin protein derived from A. actinomycetemcomitans compared with the control group. Conclusion: It can be concluded that adhesin protein derived from A. actinomycetemcomitans can cause alveolar bone damage through the increasing levels of IL-8 and MMP-8 in aggressive periodontitis.

Daya antibakteri obat kumur chlorhexidine, povidone iodine, fluoride suplementasi zinc terhadap, Streptococcus mutans dan Porphyromonas gingivalis (Antibacterial effect of mouth washes containing chlorhexidine, povidone iodine, fluoride plus zinc on Strep Betadion Rizki Sinaredi; Seno Pradopo; Teguh Budi Wibowo
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 4 (2014): December 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Dental Caries and periodontal disease prevalence in Indonesian children are still high. Some efforts can be done to overcome the problem; one of them is the use of mouthwash to decrease pathogen microorganisms. The mouthwashes that commercially available in market are chlorhexidine, povidone Iodine and Fluoride with Zinc supplementation. Purpose: The purpose of this study was to examine the anti bacterial effect of the mouthwashes chlorhexidine, povidone iodine and fluoride with zinc supplementation against mix bacteria that found in the plaque, Streptococcus mutans and Porphyromonas gingivalis. Methods: The antibacterial effect was measured using disk diffusion test. The bacteria samples (plaque polybacteria, S.mutans and P. gingivalis) were inoculated and spread in the petridish containing MHA. Paper discs containing the mouthwashes were placed in the petridish and incubated for 24 hours at 37oC (anaerobe for P. gingivalis, aerobe for S. mutans and polybacteria). The diameter of inhibition zone surrounding the paper discs were measured and compared between each active ingredient contained in mouthwash. Results: Chlorhexidine had the strongest antibacterial effect than povidone iodine and fluoride. Chlorhexidine was more effective to inhibited the growth of S. mutans than to polybacteria or P.Gingivalis, while Povidone iodine and fluoride were more effective to inhibited the growth of polybacteria. Conclusion: The mouthwash chlorhexidine was more effective to inhibit the growth of plaque polybacteria, Streptoccous mutans and Porphyromonas gingivalis compared with povidone iodine and fluoride with zinc supplementation.Latar belakang: Prevalensi karies gigi dan penyakit periodontal masih tinggi pada anak Indonesia. Usaha mengatasi hal tersebut antara lain melalui melalui penggunaan obat kumur untuk mengurangi jumlah kuman pathogen. Kandungan obat kumur yang beredar di pasar diantaranya adalah chlorhexidine, povidone iodine dan fluoride dengan suplementasi zinc. Tujuan: Penelitian ini bertujuan untuk meneliti efek antibakteri dari obat kumur berbahan aktif chlorhexidine, povidone iodine dan fluoride dengan suplementasi zinc terhadap bakteri campur plak, S. mutans dan P. gingivalis. Metode: Pengukuran efek antibakteri dilakukan dengan metode disk diffusion. Bakteri sampel (bakteri campur plak, Streptococcus mutans dan Porphyromonas gingivalis) ditanam secara merata pada cawan petri dengan medium MHA. Cakram kertas yang mengandung obat kumur diletakkan di tengah cawan petri dan diinkubasi selama 24 jam pada suhu 37o C (anaerob untuk P. gingivalis, aerob untuk S.mutans dan bakteri campur). Diameter zona hambat bakteri yang mengelilingi cakram kertas diukur dan dibandingkan antara masing-masing bahan aktif yang terkandung dalam obat kumur. Hasil: Chlorhexidine mempunyai efek antibakteri paling kuat dibanding povidone iodine dan fluoride. Chlorhexidine lebih ampuh menghambat pertumbuhan bakteri S.mutans dibanding terhadap bakteri P.gingivalis dan bakteri campur dalam plak, sedang Povidone iodine dan fluoride lebih efektif menghambat pertumbuhan bakteri campur. Simpulan: Obat kumur chlorhexidine lebih efektif dalam menghambat pertumbuhan bakteri campur dari plak, Streptococcus mutans dan Porphyromonas gingivalis dibanding povidone iodine dan fluoride dengan suplementasi zinc.

The effectiveness of mimba oil (Azadirachta indica A. Juss) spray disinfectant on alginate impression Hanoem EH; Wahjuni W; Dinda Dewi Artini
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 4 (2011): December 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Alginate impression contaminated by saliva and blood could potentially cause cross contamination. To prevent this, the impression has to be disinfected by disinfectant liquid, such as mimba oil. Mimba oil (Azadirachta indica A.Juss) has some chemical content, such as azadirachtin, which is a phenol group used as antibacterial and antimalaria, nimbolide used as antibacterial and antimalarial, and nimbidin used as antibacterial and antifungal. Purpose: The purpose of this study was to find out the most effective concentration of mimba oil as disinfectant to decrease microorganism colony on alginate impression. Methods: Thirty six samples were taken from 9 respondents. This alginate impression was divided into 4 groups: group 1 sprayed with sterile aquadest (as control group), group 2 sprayed with mimba oil 50% for 30 seconds, group 3 sprayed with mimba oil 75% for 30 seconds, group 4 sprayed with mimba oil 100% for 30 seconds. The microorganism colony was counted by colony counter. The sample data then were analyzed with Kolmogorov-Smirnov test, and was tested with Kruskal Wallis test and Mann Whitney test for further analysis. Results: There was significant difference among each group, p = 0.01 (p < 0.05). Conclusion: In conclusion, usage of 50% concentration of mimba oil as disinfectant is effective to decrease microorganism colony on alginate impression.Latar belakang: Cetakan alginat yang terkontaminasi saliva dan darah dapat berpotensi terjadinya infeksi silang. Untuk mencegah hal tersebut, cetakan didisinfeksi dengan bahan disinfektan cair seperti minyak mimba. Minyak mimba (Azadirachta indica A.Juss) memiliki beberapa kandungan kimia, antara lain Azadirachtin yang merupakan kelompok fenol yang memmiliki efek antibakteri dan antimalaria, nimbolide memiliki efek antibakteri dan antimalaria sedangkan nimbidin memiliki efek antibakteri dan antijamur. Tujuan: Tujuan dari penelitian ini adalah untuk menentukan konsentrasi yang paling efektif pada minyak mimba sebagai disinfektan untuk mengurangi jumlah koloni mikroorganisme pada cetakan alginat. Metode: Tiga puluh enam sampel diambil dari sembilan subyek. Cetakan alginat dibagi menjadi empat kelompok: kelompok 1 disemprot dengan aquades steril (kontrol), kelompok 2 disemprot dengan minyak mimba 50% selama 30 detik, kelompok 3 disemprot dengan minyak mimba 75% selama 30 detik, kelompok 4 disemprot dengan minyak mimba 100% selama 30 detik, Jumlah koloni mikroorganisme dihitung menggunakan colony counter. Data dianalisa dengan uji Kolmogorov-Smirnov, dilanjutkan dengan uji Kruskal Wallis dan Mann Whitney. Hasil: Terdapat perbedaan bermakna pada masing-masing kelompok, p = 0,01 (p < 0,05). Kesimpulan: Dapat disimpulkan dengan konsentrasi 50% sebagai desinfektan cetakan alginate telah efektif menurunkan pertumbuhan microorganisme rongga mulut.

Maturasi dan erupsi gigi permanen pada anak periode gigi pergantian (The maturition and eruption of permanent teeth in mixed dentition children) Sri Kuswandari
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 2 (2014): June 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Tooth eruption might used as predictor of child age when the chronologic age is unknown. Beside the dental maturity, tooth eruption is also influenced by some factors, such as caries and tooth extraction. Purpose: The aim of this research was to examine the relationship of maturity and eruption of permanent teeth with chronologic age in mixed dentition period children. Methods: The subjects were patients of Prof. Soedomo Dental Hospital Pediatric Dental Clinic, consist of 38 boys and 39 girls in the aged of 6-12 years. The tooth eruption data was taken by counting the permanent teeth in intra oral examination. The dental maturity was assessed by Dermijian method from dental panoramic radiology. The data were statistical analyzed by regresion-correlation and t-test program of SPSS 16.0 for Windows. Results: Maturation of permanent teeth in each of age group was more advanced in girls than boys. However, only groups of 7, 8 and 11 years were showed significant different (p<0.05), while for tooth eruption there was no significant difference (p>0.05). The coefficient correlation between tooth eruption, chronologic age and dental maturation scores were relative high, between 0.75–0.86 (p<0.01). The contribution of chronologic age and dental maturation to predict tooth eruption (R2) were 58–73.6%. The Dermijian method predicted age 0.83 years higher. Conclusion: There were close relationship between chronologic age, Dermijian method dental maturity, and eruption of permanen tooth, and could be used as predictor for eruption of permanent teeth in the mixed dentition period children. The Dermijian method predicted 0.83 years older than the chronologic age.Latar belakang: Erupsi gigi sering digunakan untuk memperkirakan umur anak. Selain maturasi gigi, erupsi gigi juga dipengaruhi oleh faktor, seperti karies dan pencabutan gigi. Tujuan: Penelitian ini bertujuan untuk meneliti hubungan antara maturasi dan erupsi gigi permanen dengan umur pada anak periode gigi bercampur. Metode: Subjek terdiri atas 38 anak laki-laki dan 39 anak perempuan berumur 5,98–11,90 tahun pasien klinik Ilmu Kedokteran Gigi Anak Rumah Sakit Gigi dan Mulut Prof. Soedomo, Fakultas Kedokteran Gigi Universitas Gadjah Mada. Penghitungan jumlah gigi permanen yang telah erupsi dilakukan dengan pemeriksaan klinis. Penentuan maturitas gigi metode Dermijian dilakukan dengan rontgen panoramik. Analisis statistik dengan regresi korelasi dan uji t program SPSS 16,0 for Windows. Hasil: Maturasi gigi permanen pada anak perempuan tiap kelompok umur lebih tinggi dibandingkan pada anak laki-laki, tetapi hanya pada kelompok umur 7, 8 dan 11 menunjukkan perbedaan bermakna (p<0,05). Tidak ada perbedaan bermakna (p>0,05) erupsi gigi antara laki-laki dan perempuan. Koefisisen korelasi antar erupsi gigi, umur kronologis, skor maturasi gigi cukup tinggi, yaitu 0,75–0,86 (p<0,01). Kontribusi umur kronologis dan maturasi gigi dalam memprediksi erupsi gigi (R2) berkisar > 58–73,6%. Metode Dermijian memprediksi umur 0,83 tahun lebih tinggi. Simpulan: Antara umur kronologis, maturasi gigi metode Dermijian dan erupsi gigi berhubungan erat, dan merupakan prediktor yang baik bagi erupsi gigi pada anak periode gigi bercampur. Metode Dermijian memprediksi 0,83 tahun lebih tinggi dibandingkan umur kronologis.

Sensitivity difference of Streptococcus viridans on 35% Piper betle linn extract and 10% povidone iodine towards recurrent apthous stomatitis Maharani Laillyza Apriasari; Bagus Soebadi; Hening Tuti Hendarti
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 3 (2011): September 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Oral ulceration often becomes the main reason for the patients to see a dentist. Therapy of the oral ulceration is by giving the palliative therapy with topical antiseptic. Nowadays, there are many researches concerning with the traditional medicines as alternative therapy. One of them is Piper betle linn which contains the antiseptic agent. Purpose: This research is aimed to observe the sensitivity difference of Streptococcus viridans on 35% Piper betle linn extract and 10%povidone iodine. Methods: This laboratory research was conducted by the post test only design with random complete design. The research sampel is Streptococcus viridans culture that was scrapped from the ulcer of the recurrent aphthous stomatitis patient, then it was replicated by using the Federer theory. Results: Inhibitory zone of 35% Piper betle linn extract is bigger than 10% povidone iodine. Conclusion: Streptococcus viridans are more sensitive to 35% Piper bittle linn extract than 10% povidone iodine. 35% Piper betle linn extract has more antibacterial effect than 10% povidone iodine.Latar belakang: Ulserasi rongga mulut seringkali menjadi alasan utama bagi pasien untuk memeriksakan diri ke dokter gigi. Terapi ulserasi rongga mulut adalah pemberian terapi paliatif kepada penderita, seperti: pemberian obat topikal yang mengandung antiseptik. Saat ini banyak penelitian dalam pengembangan obat tradisional yang dapat dijadikan sebagai obat alternatif. Salah satu diantaranya adalah daun sirih yang mengandung zat antiseptik. Tujuan: Penelitian ini bertujuan mengetahui perbedaan sensitivitas Streptococcus viridans terhadap ekstrak daun sirih 35% jika dibandingkan dengan povidone iodine 10%. Metode: Penelitian laboratoris yang dilakukan dengan post test only design dengan rancangan acak lengkap. Sampel penelitian adalah kultur Streptococcus viridans yang diambil melalui swab dari hapusan ulser pada pasien yang menderita stomatitis aftosa rekuren, kemudian dilakukan replikasi dengan rumus Federer. Hasil: Zona hambat ekstrak daun sirih 35% lebih besar daripada zona hambat povidone iodine 10%. Kesimpulan: Streptococcus viridans lebih sensitif terhadap ekstrak daun sirih 35%. Ekstrak daun sirih 35% memiliki efek daya antibakteri yang lebih tinggi jika dibandingkan dengan povidone iodine 10%.

The role of heat shock protein (HSP ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture Sri Wigati Mardi Mulyani; Ernie Maduratna Setiawati; Erma Safitri; Eha Renwi Astuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 1 (2014): March 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The concept of stem cell therapy is one of the new hope as a medical therapy on salivary gland defect. However, the lack of viability of the transplanted stem cells survival rate led to the decrease of effectiveness of stem cell therapy. The underlying assumption in the decrease of viability and function of stem cells is an increase of apoptosis incidence. It suggests that the microenvironment in the area of damaged tissues is not conducive to support stem cell viability. One of the microenvironment is the hypoxia condition. Several scientific journals revealed that the administration of hypoxic cell culture can result in stress cells but on the other hand the stress condition of the cells also stimulates heat shock protein 27 (HSP 27) as antiapoptosis through inhibition of caspase 9. Purpose: The purpose of this study was to examine the role of heat shock protein 27 as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture. Methods: Stem cell culture was performed in hypoxic conditions (O2 1%) and measured the resistance to apoptosis through HSP 27 and caspase 9 expression of bone marrow mesenchymal stem cells by using immunoflorecence and real time PCR. Results: The result of study showed that preconditioning hypoxia could inhibit apoptosis through increasing HSP 27 and decreasing level of caspase 9. Conclusion: The study suggested that hypoxic precondition could reduce apoptosis by increasing amount of heat shock protein 27 and decreasing caspase 9.Latar belakang: Konsep terapi stem cell merupakan salah satu harapan baru sebagai terapi medis kelainan kelenjar ludah. Namun, rendahnya viabilitas stem cell yang ditransplantasikan menyebabkan penurunan efektivitas terapi. Asumsi yang mendasari rendahnya viabilitas dan fungsi stem cell adalah tingginya kejadian apoptosis. Hal ini menunjukkan bahwa lingkungan mikro di daerah jaringan yang rusak tidak kondusif untuk mendukung viabilitas stem cell. Salah satu lingkungan mikro adalah kondisi hipoksia. Beberapa jurnal ilmiah mengungkapkan bahwa kondisi hipoksia pada kultur sel dapat menyebabkan sel-sel stres, namun di sisi lain kondisi stres sel juga merangsang heat shock protein 27 (HSP 27) sebagai antiapoptosis dengan menghambat ekspresi caspase 9. Tujuan: Tujuan penelitian ini adalah untuk meneliti peran protein heat shock 27 sebagai inhibitor apoptosis dalam kondisi hipoksia kultur stem cell sumsum tulang. Metode: Kultur stem sel dilakukan dalam kondisi hipoksia (O2 1%) dan mengukur resistensi terhadap apoptosis melalui ekspresi HSP 27 dan caspase 9 stem cell mesenchymal sumsum tulang dengan menggunakan immunoflorecence dan PCR real time. Hasil: Hasil penelitian menunjukkan bahwa prakondisi hipoksia dapat menghambat apoptosis melalui peningkatan HSP 27 dan penurunan tingkat Caspase 9. Simpulan: Studi ini menunjukkan bahwa prakondisi hipoksia dapat mengurangi apoptosis dengan meningkatkan jumlah protein heat shock 27 dan penurunan caspase 9.

Titanium - ceramic restoration: How to improve the binding between titanium and ceramic Harry Laksono
Dental Journal (Majalah Kedokteran Gigi) Vol. 44 No. 1 (2011): March 2011
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Titanium alloys has been used as an alternative to nickel-chromium alloys for metal-ceramic restorations because of its good biocompatibility and mechanical properties. This indicated that it was possible to design coping according to standards established for metal-ceramics. However, titanium is chemically reacting strongly with gaseous elements which causes problems when ceramics are fused to titanium. Purpose: To provide information about improving the bonding between titanium and ceramic. Review: Titanium has two crystal modifications, the close-packed hexagonal (α) structure, up to 880° C, and above this temperature the bodycentered cubic (β) structure. The principal problems is the extensive dissolution of oxygen resulting in thick, oxygen-rich titanium layers called α-case that harms the bonding of ceramic to titanium and the great mismatch in the coefficient of thermal expansion of conventional ultra-low fusing ceramic. Methods have been developed for fusing ceramic to titanium like processing methods, the used of ultra-low fusing titanium ceramic, bonding agent, and protocol for ceramic bonding to titanium. Conclusion: Titanium and titanium alloys, based on their physical and chemical properties suitable for titanium-ceramic restorations, but careful selection of processing methods, ceramic materials, laboratory skill and strict protocol for ceramic bonding to titanium are necessary to improve the bonding between titanium and ceramic.Latar Belakang: Logam campur titanium telah dipakai sebagai salah satu bahan alternatif untuk logam nikel-krom pada pembuatan restorasi keramik taut logam karena mempunyai biokompatibilitas dan sifat mekanik yang baik. Hal ini menunjukkan bahwa logam titanium dapat dipakai untuk pembuatan koping logam berdasarkan standar yang dipakai untuk pembuatan restorasi keramik taut logam. Meskipun, secara kimiawi logam titanium bereaksi dengan elemen-elemen gas yang menyebabkan masalah pada perlekatan keramik pada titanium. Tujuan: Memberikan informasi tentang cara meningkatkan kekuatan perlekatan antara keramik dengan titanium. Tinjauan: Titanium mempunyai 2 struktur kristal, struktur close-packed hexagonal (α) diatas 880°C dan struktur bodycentered cubic (β) dibawah 883°C. Masalah utama adalah pelepasan gas oksigen yang menghasilkan lapisan titanium kaya oksigen yang tebal disebut α-case yang menghalangi perlekatan keramik pada titanium dan koefisien ekspansi panas dari bahan ultra-low fusing ceramic yang berbeda dengan titanium. Berbagai cara telah dikembangkan untuk mendapatkan perlekatan keramik pada titanium seperti teknik pembuatan, pemakaian bahan ultra-low fusing titanium ceramic, bahan bonding dan protokol untuk perlekatan bahan keramik pada titanium. Kesimpulan: Titanium dan logam campur titanium, berdasarkan sifat-sifat mekanik dan kimiawinya dapat dipakai untuk pembuatan restorasi keramik taut logam, tetapi pemilihan teknik pembuatan, bahan keramik, ketrampilan peteknik gigi dan mengikuti protokol untuk mendapatkan perlekatan keramik pada titanium dengan benar diperlukan untuk meningkatkan kekuatan perlekatan antara keramik dan titanium.

Korelasi antara jumlah mikronukleus dan ekspresi 8-oxo-dG akibat paparan radiografi panoramic (The correlation of micronucleus formation and 8-oxo-dG expression due to the panoramic radiography exposure) Rurie Ratna Shantiningsih; Suwaldi Suwaldi; Indwiani Astuti; Munakhir Mudjosemedi
Dental Journal (Majalah Kedokteran Gigi) Vol. 46 No. 3 (2013): September 2013
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The expression of 8-oxo-dG is defined as one form of damaged DNA occuring as the result of oxidation reaction due to X ray exposure. Panoramic radiography exposure has been widely known to be able to increase micronucleus which are signing the early stage and as biomarker in carsinogenesis mechanism. Purpose: The purpose of this research was to determine the correlation between micronucleus number and 8-oxo-dG expression as a result of panoramic radiography exposure. Methods: Twelve New Zealand male rabbits aging 6 months were divided into 4 groups. Group I were rabbits that represented 0 day, group II represented 3th day, group III represented 6th day, and group IV represented 9th day after the panoramic radiography exposure. Respectively samples were swabbed at mandibular anterior gingival mucosa before and after the panoramic radiography exposure. The swabbed samples were coloured by using Feulgen-Rossenbeck modified staining for calculating the amount of micronucleus formation. Expression of 8-oxodG was detected using immunohistochemical of rabbit’s gingival mucosa epithelium. Statistical analysis were carried out on pearson correlation. Results: There was a highest increasing of micronucleus on the 9th day after panoramic radiography exposure. Meanwhile, there was significant correlation (p=0,049) of the increasing amount of micronucleus and 8-oxo-dG expression in negatively correlation (r = -0,578). The increasing of micronucleus formation on the 9th day after panoramic radiography exposure was accordance with some previous studies. The expression score of 8-oxo-dG decreases as the day goes by. Conclusion: There was a correlation between the number of micronucleus and expression score of 8-oxo-dG.Latar belakang: Ekspresi 8-oxo-dG adalah suatu bentuk kerusakan DNA yang terjadi akibat reaksi oksidatif dari paparan sinar X. Paparan radiografi panoramik telah diketahui menyebabkan peningkatan jumlah mikronukleus yang merupakan biomarker tahap dini mekanisme karsinogenesis. Tujuan: Tujuan penelitian ini adalah untuk meneliti korelasi antara jumlah mikronukleus dan ekspresi 8-oxo-dG akibat paparan radiografi panoramik. Metode: Sebanyak 12 ekor kelinci New Zealand jantan usia 6 bulan dibagi menjadi 4 kelompok. Kelompok I merupakan kelinci untuk mewakili hari ke-0, kelompok II untuk mewakili hari ke-3, kelompok III untuk mewakili hari ke-6, dan kelompok IV untuk mewakili hari ke-9 setelah paparan radiografi panoramik. Terhadap seluruh kelinci dilakukan usapan pada mukosa gingiva anterior rahang bawah sebelum dan sesudah dilakukan paparan radiografi. Selanjutnya terhadap sampel usapan itu dilakukan pewarnaan menggunakan teknik pewarnaan modifikasi Feulgen-Rossenbeck untuk menghitung jumlah mikronukleus. Ekspresi 8-oxo-dG dinilai dari pemeriksaan imunohistokimia pada sel epitel mukosa gingiva kelinci. Analisis statistik menggunakan uji korelasi Pearson. Hasil: Peningkatan jumlah mikronukleus paling tinggi terjadi pada hari ke-9 setelah paparan radiografi panoramik. Sementara itu, terdapat korelasi yang signifikan (p=0,049) antara peningkatan jumlah mikronukleus dan ekspresi 8-oxo-dG dengan arah korelasi yang negatif (r=-0,578). Peningkatan jumlah mikronukleus yang terjadi pada hari ke-9 setelah paparan radiografi panoramik ini sesuai dengan penelitian sebelumnya. Dengan bertambahnya hari skor ekspresi 8-oxo-dG semakin menurun. Simpulan: Terdapat korelasi antara jumlah mikronukleus dan skor ekspresi 8-oxo-dG.

Candida albicans adherence on acrylic resin plates immersed in black tea steeping Soebagio Soebagio
Dental Journal (Majalah Kedokteran Gigi) Vol. 43 No. 4 (2010): December 2010
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Black tea or Cournelia sinensis is one of known tea varieties in Indonesia. Actually, black tea is consumed daily as beverage that can function as antiseptics and fungicides. Black tea containing antibacterial and fungicide properties can reduce the number of Candida albicans (C. albicans) colony attachment on the surface of acrylic resin plates. Purpose: This study was done to determine the effective concentration of black tea steeping used as the immersion material of acrylic resin plates towards the number of Candida albicans colonies. Methods: In this study, acrylic resin plates with the size of 10 × 20 × 1 mm were immersed in black tea steeping with the following concentrations, 3.33%, 6.66%, and 13.33%, for one hour. The growth of C. albicans colonies were then detected by counting the number of colonies growing on Sabouraud’s dextrose medium. Data was analyzed by using both One-Way ANOVA with the significance level of 5%, and Tuckey’s multiple comparison tests (Tuckey’s HSD test). Results: There were significant differences in the number of C. albicans colonies in acrylic resin plates immersed in black tea with the concentration of 3.33%, 6.66%, and 13.33%. Conclusion: It can be concluded that the higher concentration of black tea (13.33%) used to immerse acrylic resin plates, the greater the decreased number of C. albicans colonies.Latar belakang: Teh hitam atau Cournelia sinensis adalah salah satu macam teh yang dikenal di Indonesia. Teh hitam seringkali dipakai sebagai minuman sehari- hari dan berkhasiat sebagai antiseptik maupun fungisid. Teh hitam yang mengandung sifat antibakteri dan fungisid dapat menurunkan jumlah koloni Candida albicans (C. albicans) yang melekat pada permukaan lempeng resin akrilik. Tujuan: Untuk mengetahui konsentrasi efektif seduhan teh hitam sebagai bahan perendam lempeng resin akrilik terhadap jumlah koloni C. albicans. Metode: Pada penelitian ini lempeng resin akrilik ukuran 10 × 20 × 1 mm direndam dalam seduhan teh hitam konsentrasi 3,33%, 6,66%, 13,33% selama 4 jam. Pertumbuhan jumlah koloni C. albicans dilakukan dengan cara menghitung jumlah koloni yang tumbuh pada media Sabouraud’s dextrose. Analisis data menggunakan uji anova satu arah dengan taraf kemaknaan 5%, uji pembanding ganda tuckey HSD. Hasil: Terdapat perbedaan yang bermakna jumlah koloni C. Albicans yang melekat pada lempeng resin akrilik yang direndam dalam seduhan teh hitam dengan konsentrasi 3,33%, 6,66% dan 13,33%. Kesimpulan: Konsentrasi 13,33% atau makin pekat seduhan teh hitam yang digunakan untuk merendam lempeng resin akrilik menyebabkan penurunan jumlah koloni C. albicans.

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Home Page

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Contact Name
Saka Winias

Contact Email
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Location
Kota surabaya,

Jawa timur

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