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INDONESIA
The Indonesian Biomedical Journal
Published by The Prodia Education and Research Institute
ISSN : -     EISSN : -     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Public Health
Arjuna Subject : -
Articles 15 Documents
 1   2 
Search results for , issue "Vol 10, No 3 (2018)" : 15 Documents clear
Screening of Extended-Spectrum β-Lactamases (ESBL)-producing Klebsiella pneumoniae with ChromID ESBL Media Emy Noerwidayati; Andaru Dahesihdewi; Osman Sianipar
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.430

Abstract

BACKGROUND: Klepsiella pneumoniae, one of clinical isolates, is frequently found causative agent of hospital acquired infection. Currently, K. pneumoniae is found as extended-Spectrum β-lactamases (ESBL) producer, allowing it to become multidrugresistant. A clinical laboratory with limited facility needs a valid, reliable, inexpensive and simple laboratory test to control its infection and antimicrobial-resistancy. The aim of this study is to evaluate the diagnostic performance of a ESBL media to detect ESBL-producing K. pneumoniae.METHODS: An independent and blind comparative study of ChromID ESBL media and Double Disc Synergy Test (DDST) was conducted for detecting the clinical isolate of ESBL-producing K. pneumoniae. Clinical isolates of K. pneumoniae collected from the Clinical Laboratory of Dr. Sardjito Hospital were isolated.RESULTS: There were 103 clinical isolates of K. pneumoniae, which were isolated from urine, pus, blood, stool, cerebrospinal fluid, sputum, drain liquid, nasal sinus liquid, gastric wash, bronchi liquid, injury liquid and nasal swab. The number of true positive, true negative, false positive and false negative results were 74, 18, 9 and 2, respectively. Meanwhile, the sensitivity, specificity, positive predictive value, negative predictive value, likelihood ratio for positive result and likelihood ratio for negative result of the new ESBL media were 97.4%, 66.7%, 89.2%, 90%, 2.9 and 0.03, respectively.CONCLUSION: Since the new ESBL media and DDST results were similar, so the new ESBL media could be used for screening patients with clinical presentation that indicating a high suspicious of ESBL-producing bacteria.KEYWORDS: K. pneumoniae, ChromID ESBL, DDST, ESBL, sensitivity
Detection of Polymorphism on Voltage-gated Sodium Channel Gene of Indonesian Aedes aegypti Associated with Resistance to Pyrethroids Budi Mulyaningsih; Sitti Rahmah Umniyati; Tri Baskoro Tunggul Satoto; Ernaningsih Ernaningsih; Dwi Aris Agung Nugrahaningsih
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.425

Abstract

BACKGROUND: Aedes aegypti is a vector of several pathogens including dengue virus. Vector control is an effective way to break the transmission but unfortunately constant use of insecticides ultimately causes vector resistance. Pyrethroids have been used for about 15 years to combat Ae. aegypti in Yogyakarta Province, Indonesia. Single amino acid substitutions in the voltagegated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance (kdr). The aim of this study is to detect resistant status to cypermetrine and polymorphism on the voltagegated sodium channel gene of Ae. aegypti from 2 dengue endemic areas in Yogyakarta Province (Yogyakarta city and Sleman district).METHODS: Pyrethroid resistance in Ae. aegypti mosquitoes was detected by using CDC Bottle Bioassay. To detect the polymorphism on the voltage-gated sodium channel gene of Ae. aegypti analyses were conducted by using PCR and direct DNA squencing with primers AaSCF1 and AaSCR4 for S989P, I1011M (or V), L1014F sites, and AaSCF7 and AaSCR7 for the F1534C site.RESULTS: According to bioassay, the results for Ae. aegypti from Yogyakarta city (93% mortality) and Sleman district (88% mortality) suggest the possibility of resistance to cypermethrin. We observed polymorphism on voltagegated sodium channel gene on site F1534C (heterozygous).CONCLUSION: The findings provide early evidence that the use of cypermethrin (pyrethroids) in Yogyakarta city and Sleman district, Yogyakarta Province, Indonesia is reducing its effectiveness to control Ae. aegypti. Recommendations include additional tests for confirmation.KEYWORDS: Aedes aegypti, dengue virus, cypermethrin, Bioassay, Yogyakarta
Phylogeny of HPV-16 and HPV-18 Multiple Infection of a Patient with Cervical Cancer from Dr. Hasan Sadikin General Hospital, Bandung: A Case Report Vera Amalia Lestari; Ika Agus Rini; Gita Widya Pradini; Edhyana Sahiratmadja; Herman Susanto
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.383

Abstract

BACKGROUND: From all of human papillomaviruses (HPV) genotypes capable of causing cervical cancer, it is estimated that 70 percent are HPV-16 and HPV-18. HPV-16 can infect the tissues in single infection or together with other high-risk types of HPV, and the most common is with HPV-18. The origin of HPV can be identified by its phylogenetic tree. The aim of this study was to determine the phylogeny of HPV-16 and HPV-18 multiple infection in cervical cancer, whether both HPVs were from the same origin.METHODS: Cervical tissue biopsies (n=33) were obtained from Hasan Sadikin Hospital in the period of September to November 2016. HPV genotyping test was performed to confirm the HPV-16 and HPV-18 multiple infection. L1 gene of both HPV-16 and HPV-18 were sequenced for phylogenetic analysis.RESULTS: Phylogenetic analysis of L1 HPV-16 and HPV-18 showed the closest relationship with sequence from China and Thailand, respectively.CONCLUSION: HPV-16 and HPV-18 multiple infection of a cervical cancer patient from Dr. Hasan Sadikin General Hospital Bandung showed a very close L1 phylogeny relationship with isolate from Asian region.KEYWORDS: HPV-16, HPV-18, multiple infection, cervical cancer, Bandung
High VEGF Level is Produced by Human Umbilical Cord- Mesenchymal Stem Cells (hUC-MSCs) in Amino Acid-Rich Medium and under Hypoxia Condition Veronika Maria Sidharta; Elizabeth Henny Herningtyas; Christine Ayu Lagonda; Dilafitria Fauza; Yuyus Kusnadi; Rina Susilowati; Ginus Partadiredja
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.457

Abstract

BACKGROUND: Secretome production by stem cells depends on their culture conditions such as oxygen concentration and the composition of the culture media. In this study, we investigated the secretion of neurotrophic growth factors of human umbilical cord mesenchymal stem cells (hUC-MSCs) in amino acid-rich culture medium and under hypoxic condition.METHODS: hUC-MSCs were cultured in normoxic and various hypoxic (1%, 5%, 10%) conditions in an amino acid-rich culture medium. The end-point parameters (cell proliferation and survival, cell morphology and growth factor secretion) were measured at 3 time-points (48 hours, 72 hours and 96 hours). ELISA-based methods were used for neurotrophic factors detection, including neurotrophic growth factor (NGF), vascular endothelial factor (VEGF), and brain-derived neurotrophic factor (BDNF).RESULTS: NGF secretion was not detectable at any time points both in normoxia and hypoxia. BDNF secretion under normoxia was induced at 48 h time point and reached the highest level at an average of 181.9±13.01 pg/mL at 96 hours, whereas hypoxia exposure to hUC-MSCs only induced the BDNF secretion at low level. VEGF secretion was barely detectable in normoxic condition. However, VEGF secretion reached the highest level at an average of 7707.55±2110.85 pg/mL in 5% hypoxia at 96 hours.CONCLUSION: Combination of amino acid-rich culture medium and hypoxia condition dramatically induced high VEGF secretion by hUC-MSCs, especially at 5% hypoxia, induced mild BDNF secretion and had no effect toward NGF secretion.KEYWORDS: human umbilical cord mesenchymal stem cells, neurotrophic growth factor, amino acid-rich, hypoxia
Analysis of ALDH1A1 and ALDH1A3 Gene mRNA Expressions in Adipose-Derived Stem Cells (ASCs) and Umbilical Cord Stem Cells (UCSCs) Septelia Inawati Wanandi; Purnamawati Purnamawati; Alice Tamara; Karina Teja Putri; Daniel Marcellius Simadibrata
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.477

Abstract

BACKGROUND: ALDH1A1 and ALDH1A3, the most renowned isozymes of aldehyde dehydrogenase (ALDH)1, are important in regulating the pluripotency of human mesenchymal stem cells (MSCs) and cancer stem cells (CSCs). The study aimed to analyze the mRNA expression of ALDH1A1 and ALDH1A3 genes in adipose stem cells (ASCs) and umbilical cord stem cells (UCSCs) along with their correlations to Oct-4 mRNA expression. Additionally, the interaction between these proteins was also investigated using in silico study to confirm the pluripotency of both MSCs compared to human breast ALDH+ CSCs.METHODS: This research focused on determining mRNA levels of ALDH1A1, ALDH1A3 and Oct-4 in ASCs and UCSCs using one-step qRT-PCR. The data were then normalized to those in human breast CSCs and 18S rRNA. Oct-4 gene expression was also analyzed to determine the pluripotency of ASCs and UCSCs. The protein-protein interactions were in silico analyzed using String 9.1 software.RESULTS: Relatively, ALDH1A3 was expressed at similar level in ASCs and UCSCs, while ALDH1A1 expression level was significantly higher in ASCs compared to UCSCs. In contrast to ALDH1A3, the expressions of ALDH1A1 in both MSCs were significantly lower than breast CSCs similar to Oct-4 expressions, as also revealed by the in silico data showing the interaction between these proteins. This suggests the role of ALDH1A1 on pluripotency.CONCLUSION: ALDH1A1 and ALDH1A3 were distinctly expressed in UCSCs and ASCs, which might be associated with unique properties of ASCs and UCSCs. This study may contribute to further research in terms of implication of ALDH1A1 and ALDH1A3 expressions towards the properties of MSCs and its application in stem cell therapy.KEYWORDS: ALDH1A1, ALDH1A3, UCSC, ASC, breast ALDH+ CSC, Oct-4
Positive Correlation between Very Small Embryonic Stem Cell, Hematopoietic Stem Cell, and Endothelial Progenitor Cell in Umbilical Cord Blood Unit Angliana Chouw; Bayu Winata Putera; Cynthia Retna Sartika; Ajeng Diantini
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.455

Abstract

BACKGROUND: Umbilical Cord Blood (UCB) has been widely use as regenerative medicine due to the content of undifferentiated cell which have capability to do self-renewal and differentiation into various type of cell called stem cells. Recent studies show that UCB contains not only hematopoietic stem cell (HSC) but also others stem cell and progenitor cell such as endothelial progenitor cell (EPC) and very small embryonic-like stem cell (VSEL). It is beliefs that HSC and EPC shared the same progenitor. In this study, correlation between the cell number of HSC, EPC and VSEL is analyzed in umbilical cord blood as the source of stem cell for clinical application.METHODS: The cell number of HSC, EPC and VSEL is counted from cryopreserved UCB collected from 22 women delivered via cesarean section which already stored for more than 2 years in this study. Sample were incubated with antibodies such as cluster of differentiation (CD)34-phycoerythrin (PE)/CD45-fluorescein isothiocyanate (FITC), CD133/1 (anti-CD (AC)133)-antigen-presenting cell (APC) and, CD184 (C-X-C chemokine receptor (CXCR)4)-PE.Vio770 to detect the present of HSC, EPC and VSEL in UCB. Sample were analyze using flowcytometer BD FACS Canto II.RESULTS: The cell population of HSC and late-EPC is 0.009% and 0.01% of total cell in UCB. VSEL only represented 0.001% from total cell in UCB, showing the lowest number of cell population in UCB. The correlation between the cell number of HSC and EPC is r=0,483*, p=0.023) and between HSC and VSEL is r=0.510*, p=0.015.CONCLUSION: In this study, both EPC and VSEL have a significant positive correlation with HSC.KEYWORDS: stem cell, umbilical cord blood, endothelial progenitor, flowcytometry
The Effect of Estradiol on Renal Function in Reversible and Irreversible Unilateral Ureteral Obstruction in Rats Zahra Lak; Akbar Vahdati; Mehdi Nematbakhsh
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.459

Abstract

BACKGROUND: The ureteral obstruction (UO) is the most common clinical disorders. This study was designed to investigate the renoprotective effect of estradiol in unilateral UO (UUO) and reversible UUO (RUUO).METHODS: Eighty-four ovareictomized Wistar rats were assigned into 14 groups. Group 1 was sham operated group. All the groups 2-14 were subject to UUO, however they received additional treatments. Group 2-4 received test substances (vehicle, 0.1 or 0.05 mg/kg of estradiol) and sacrificed after 3 days. Group 5-7 received test substances and after 3 days followed by RUUO (obstruction removal) and after one day post RUUO were sacrificed. Group 8-9 followed by RUUO after 3 days and simultaneously received estradiol and after one day post RUUO were sacrificed. Group 10-12 followed by RUUO after 3 days and simultaneously received test substances and after 3 days post RUUO were sacrificed. Group 13-14 received test substances followed by RUUO and received test substances again and after 3 days post RUUO were sacrificed.RESULTS: The estradiol reduced body weight significantly (p<0.05) in UUO, and the uterus weight (UW) increased significantly by estradiol (p<0.05). The increased serum levels of blood urea nitrogen (BUN) and creatinine (Cr), and kidney weight (KW) induced by UUO (p<0.05) were not attenuated by estradiol. Estradiol treatment did not alter the levels of BUN, Cr, KW, Cr clearance (CrCl), urine sodium excretion and urine flow when compared with control.CONCLUSION: Although the protective role of estradiol in cardiovascular system is well accepted, however our findings suggest that its protection during UUO or after RUUO in renal system cannot be certain.KEYWORDS: estradiol, ureteral obstruction, renal function, rat
Frequencies of CYP1A2 Single Nucleotide Polymorphism in Indonesian and Its Effect on Blood Pressure Erizal Sugiono; Andi Wijaya; Anwar Santoso; Ferry Sandra; Ilham Jaya Patellongi; Irawan Yusuf
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.374

Abstract

BACKGROUND:  The association between caffeine with blood pressure (BP) still remains controversial. Caffeine is mainly metabolized by cytochrome-P450 (CYP)1A2 enzyme. Polymorphism of CYP1A2 is known to cause interindividual variation on enzymatic activity, thus affects caffeine metabolism and its effect on cardiovascular (CV) system.METHODS: We conducted a cross-sectional study and recruited 121 Indonesian subjects aged 25-60 years with varying coffee-drinking habits. DNA was extracted from peripheral blood and genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique. Blood pressures were measured in the morning prior to the daily activity. Caffeine concentration in blood plasma was measured using high-performance liquid chromatography (HPLC). The differences between variables were analyzed using MannWhitney analysis and the correlations among variables were determined using multivariate logistic regression analysis.RESULTS: This study showed that the frequencies of single nucleotide polymorphisms (SNPs) among Indonesian were 31.8%, 18.2%, 25.2% and 24.8% respectively for CYP1A2*1A, CYP1A2*1B, CYP1A2*1C and CYP1A2*1F alleles. The genotype analysis showed that the subject number of the wild type (*1A/*1A) and the variants were 9.92% and 90.08%. There were no significant differences in term of BP levels among CYP1A2 genotypes and coffee drinking habit groups.CONCLUSIONS: The frequencies of CYP1A2 SNPs in Indonesian are different with frequencies in other populations. Since the association were not statistically significant, CYP1A2 polymorphisms as the predictor of elevated blood pressuare should be investigated further.KEYWORDS: coffee, caffeine, blood pressure, single nucleotide polymorphism, CYP1A2
The dtxR Gene: A New Alternative Marker to Identify Corynebacterium ulcerans and Corynebacterium pseudotuberculosis by PCR Assay Sunarno Sunarno; Nyoman Fitri; Nelly Puspandari; Kambang Sariadji
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.403

Abstract

BACKGROUND: There are found some studies which reported the successfull of polymerase chain reaction (PCR) assay to identify Corynebacterium ulcerans and Corynebacterium pseudotuberculosis. This study aimed to describe the dtxR gene profile as a new marker for C. ulceran and C. pseudotuberculosis for the PCR assay.METHODS: Ten C. ulcerans and 35 C. pseudotuberculosis DNA sequences data registered in GeneBank was analyzed by bioinformatic tools. PCR primer was designed based on the concerved region and the gene similarity data. On the other hands, reference strains (C. ulcerans NCTC 12077 and Corynebacterium diphtheriae NCTC 3984) and dtxR gene of C. pseudotuberculosis (synthetic gene) were used in the PCR assay optimization for C. ulcerans and C. pseudotuberculosis identification.RESULTS: The study showed that dtxR genes of both C. ulcerans and C. pseudotuberculosis were more conserve than pld gene, moreover dtxR gene was more specific compared to 16S rRNA gene. PCR assay with dtxR gene as a target could identify C. ulcerans and C. pseudotuberculosis accurately without mispriming, misamplification and misidentification.CONCLUSION: dtxR gene could be used as marker to identify C. ulcerans and C. pseudotuberculosis by PCR assay.KEYWORDS: C. pseudotuberculosis, C. ulcerans, dtxR gene, PCR
Could Complete Blood Count Parameters and Non-fasting Cholesterol Profile Describe Inflammation and Oxidative Stress in Chronic Kidney Disease? Ika Nindya Kadariswantiningsih; Mochammad Thaha; Cahyo Wibisono Nugroho; Berliana Hamidah; Haerani Rasyid; Zaky El Hakim; Maulana Muhtadin Suryansyah; Mohammad Yusuf Alsagaff; Djoko Santoso; Maulana Antiyan Empitu; Yusuke Suzuki
The Indonesian Biomedical Journal Vol 10, No 3 (2018)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v10i3.451

Abstract

BACKGROUND: Establishment of inexpensive clinical laboratory tests to evaluate inflammation and oxidative stress is urgently needed in the limited resources settings. This study aims to investigate the potential of complete blood count (CBC) parameters and non-fasting cholesterol profile parameters to describe inflammation and oxidative stress in chronic kidney disease (CKD) patients.METHODS: Measurement of CBC, non-fasting cholesterol profile, high sensitivity C-reactive protein (hs-CRP) and malondialdehyde (MDA) were performed in 71 CKD patients grouped into hemodialysis (HD) and non-hemodialysis (non-HD). Correlation analysis were performed to assess the potential of CBC and cholesterol profile to describe the level of hs-CRP and MDA.RESULTS: In the HD group, total cholesterol was moderately associated with hs-CRP while total cholesterol/HDL-C ratio, monocyte/HDL-C ratio, monocyte/LDL-C ratio, neutrophil/HDL-C ratio, neutrophil/LDL-C ratio, platelet/HDL-C ratio and platelet/LDL-C ratio were strongly associated with hs-CRP. In the non-HD group,only neutrophil/total cholesterol ratio and platelet/total cholesterol ratio that were associated with hs-CRP. Total cholesterol, monocyte/LDL-C ratio, neutrophil/LDL-C ratio and platelet/LDL-C ratio were moderately associated with MDA while total cholesterol/HDL-C ratio, monocyte/HDL-C ratio, neutrophil/HDL-C ratio and platelet/HDL-C ratio were strongly associated respectively with MDA in HD group. In the non-HD group, total cholesterol/HDL-C ratio, neutrophil/HDL-C ratio and platelet/HDL-C ratio were moderately associated with MDA in non-HD group while monocyte/HDL-C ratio was weakly associated with MDA.CONCLUSION: Some CBC parameters and non-fasting cholesterol profile such as cholesterol/HDL-C, monocyte/HDL-C, neutrophil/HDL-C and platelet/HDL-C ratio showed a potential to describe the inflammation and stress oxidative markers, given some strong associations with the level of hs-CRP and MDA. Further study is needed to assess whether this parameter represent long-term prognostic value among CKD patients.KEYWORDS: inflammation, oxidative stress, CRP, MDA, TAC, 8-OHdG, CBC, cholesterol

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