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CHARACTERIZATIONS OF Trichoderma sp. AND ITS EFFECT ON Ralstonia solanacearum OF TOBACCO SEEDLINGS Sutarman, Sutarman; Jalaluddin, Ahmad Khafidh; Li’aini, Arrohmatus Syafaqoh; Prihatiningrum, Andriani Eko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol. 21 No. 1 (2021): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.1218-19

Abstract

Characterizations of Trichoderma sp. and its effect on Ralstonia solanacearum of tobacco seedlings. This study aims todetermine the molecular-based characteristics of Trichoderma sp. Tc-Jjr-02 and its effect as a biocontrol agent in protecting tobaccoseedlings against bacterial wilt caused by R. solanacearum. The characterization of biocontrol agents was based on morphologicaland molecular data’s observation using microscope and the key of determination. The in vivo experiments was consist of fivetreatments: (1) inoculation of Trichoderma isolates at six hours before R. solanacearum inoculation, (2) inoculation of Trichodermaisolates at six hours after R. solanacearum inoculation (3) simultaneous inoculation of Trichoderma isolates and R. solanacearum,(4) inoculated only with R. solanacearum, and (5) without any inoculation. The experiment was repeated six times. Based onBLAST’s analysis, the Tc-Jjr-02 sequence is in accordance with T. asperellum with 100% Query Cover. Inoculation of T. asperellumTc-Jjr-02 at six hours before and after and simultaneously with pathogens providing protection for young tobacco plants by slowingdown the time for the onset of blight by 100–162%, reducing the symptom index by 56–63%, and increasing the dry weight of plantbiomass by 39–53% compared to tobacco seeds which were only inoculated with R. solanacearum.
PENYAKIT HAWAR DAUN PINUS MERKUSII DI BERBAGAI PERSEMAIAN KAWASAN UTAMA HUTAN PINUS JAWA TIMUR Sutarman .; Andriani Eko Prihatiningrum
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.166 KB) | DOI: 10.23960/j.hptt.11544-52

Abstract

Leaf blight disease of Pinus merkusii in various nurseries of pine forest in major area of East Java. This study aims to: (i) obtain isolates of pathogens, (ii) determine disease on seedling growth during the critical period and the young plants, (iii) determine the effect of pathogenic isolates on disease severity, and (iv) determine the effect of altitude on severity of seedling’s leaf blight disease of Pinus merkusii from various nursery locations in East Java. The experiment was conducted in February-August 2014 at nine locations in the nursery and one young pine plantation site. Disease index data were analyzed using Microsoft Office Excel 2007 software to determine the regression model of the relationship between the index of disease with the independent variables and with Anova followed by Duncan test to find out the effect of altitude difference to the index of disease. Pathogen that causes late blight is Pestalotia theae Sawada. In the critical period, seedling disease index increased from 7.38 to an average of 26.96 in the nursery RPH Celaket; while on the young plants in the field in RPH Kemiri disease index ranged 28-31. Differences in altitude did not affect disease index difference. Based on disease index, three isolates of pathogens that require serious attention are Wagir isolates (985 m asl., the most virulent), Ngantang (500 m asl.), and South Pujon (1200 m asl.).
Dukungan Sosial dan Perilaku Sehat Guru Inisiator Sekolah Adiwiyata widyastuti widyastuti; Lely Ika Mariyati; Andriani Eko Prihatiningrum
Jurnal Pesut : Pengabdian untuk Kesejahteraan Umat Vol 1 No 2 (2019): Jurnal Pesut: Pengabdian untuk Kesejahteraan Umat
Publisher : Pusat Penerbitan Ilmiah Universitas Muhammadiyah Kalimantan Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30650/jp.v1i2.700

Abstract

Schools have large potential of human resources. Unfortunately, this potential has not effectively supported the Sekolah Adiwiyata program. Two junior high schools in Tulangan Subdistrict, Sidoarjo Regency, have potential human resources that can be optimized in initiating the Sekolah Adiwiyata program. Post-training training and mentoring were provided as a form of social support for the initiation of the Sekolah Adiwiyata program. The results show that social support strengthens the teacher's healthy behavior as the initiator of the Sekolah Adiwiyata.
PKM Karang Taruna Desa Pagerngumbuk Kecamatan Wonoayu Kabupaten Sidoarjo Propinsi Jawa Timur Emy Rosnawati; Andriani Eko Prihatiningrum; Lely Ika Mariyati
SNHRP Vol. 2 (2019): Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Ke 2 Tahun 2019
Publisher : LPPM Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.397 KB)

Abstract

Desa pagerngumbuk merupakan desa Administrasi yang terletak di Kecamatan Wonoayu Kabupaten Sidoarjo untuk dikembangkan adalah masalah lingkungan. Permasalahan yang dihadapi oleh mitra di Desa Pagerngumbuk khususnya RT 02 RW 03 adalah:1) Minimnya kesadaran pentingnya lingkungan hidup bersih dan sehat. 2) Kurang memiliki sarana dan prasarana pendukung terciptanya lingkungn hidup yang bersih dan sehat seperti ; bak sampah, bank sampah 3) Apabila musim hujan sering terjadi banjir sehingga merusak tanaman sawah 4) Banyak tanah kosong yang kurang produktif sehingga kurang bermanfaat 5) kurang memiliki pemahaman dan ketrampilan yang memadai dalam manajemen pengelolaan lingkungan hidup seperti pengelolaan sampah. Tujuannya menciptakan lingkungan yang bersih dan sehat serta pemanfaatan sampah untuk nilai ekonomis. Metode kegiatan berupa pelatihan dan pengadaan barang. Dampak dari pengabdian ini adalah peningkatan pengetahuan terkait perilaku hidup sehat dan peningkatan ketrampilan pengurus dalam mensosialisasikan perubahan perilaku sehat dan managemen pengolahan sampah. Hasil pengabdian masyarakat menunjukkan adanya perubahan dan mempunyai pengaruh yang positif dibuktikan warga sudah membiasakan diri membuang sampah pada tempatnya dan memanfaatkan sampah anorganik menjadi kerajinan yang bernilai ekonomis . Kata kunci : lingkungan, bersih, sehat
The Effect of Liquid Organic Fertilizer Concentration on Growth and Production of Pakcoy Mustard (Brassica rapa L.) Muhammad Irfan Hakim; Andriani Eko P
Procedia of Engineering and Life Science Vol 1 No 1 (2021): Proceedings of the 1st Seminar Nasional Sains 2021
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (550.466 KB) | DOI: 10.21070/pels.v1i1.757

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This research aims to find out the influence of liquid organic fertilizer concentration on the growth and production of mustard pakcoy (Brassica rapa L.). The research was conducted in Jiken village, Tulangan District, Sidoarjo Regency and plant physiology laboratory of Muhammadiyah University of Sidoarjo in July to September 2020. This research using RandomIzed Group Design (RAK) was compiled in a single factor, namely the concentration of liquid organic fertilizer with a level of 25%, 50%, 75% and repeated 3 times so that there are 30 units of experiments. The observed variables are: plant height (cm), leaf area (cm2), number of leaves (strands), wet weight (grams), antioxidant activity (ppm). The data was analyzed using ANOVA followed by BNJ test at 5% test level. The results showed no noticeable influence on the variable height of the plant and the area of the leaves. In variable number of leaves and wet weight there is a noticeable influence on the treatment of liquid organic fertilizer concentration 75% application as much as 4 times at the observation age of 35 days with the average yield of the number of leaves (17.33 strands) and wet weight (92 grams). Antioxidant activity tests showed the best results in the control treatment with the lowest value (174,779 ppm) because the lower the IC50 value, the better the antioxidant activity.
Training on processing household organic waste into eco enzyme at 'Aisyiyah Sidoarjo Orphanage Syamsudduha Syahrorini; Andriani Eko Prihatiningrum; Akhmad Mulyadi; Ida Agustini Saidi
Community Empowerment Vol 7 No 11 (2022)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31603/ce.7359

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The catering operation at the Aisyiyah Sidoarjo Orphanage generates about 5-7 kg of organic waste each day. The orphanage does not currently have a waste segregation site, which means that waste is mixed together and wrapped in plastic, which is obviously bad for the environment. In order to maintain cleanliness and environmental health and to improve the orphanage's economy by using Eco Enzyme fluids, the community service's objective is to give orphanage children early training in the processing of organic waste into Eco Enzyme. The method is through lectures and the practice of making eco enzymes. This program produces Eco Enzyme and its fermentation, which can be used to make liquid plant fertilizer, treat burns and itching, clean bathrooms and floors, and dish soap-and-liquid bath soap blend.
The Effect Of Planting And Volume Media Soaking Time Zpt Plant Seed On The Growth Beginning Of Cane ( Saccharum Officinarum L. ) System Bud Chip Andriani Eko Prihatiningrum; Moch R Syarifuddin Fathony
Nabatia Vol 5 No 1 (2017): June
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.114 KB)

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The study aims to determine the effect of interaction between the treatment effect of soaking tanamdan media volume ZPT against early seedling growth of sugarcane (Sac- charum officinarum L) system bud chip, green hause conducted at the University of Muhammadiyah Sidoarjo in November to January 2016. The study used randomized block design pola faktorial, consisting of two factors and three replications. The first fac- tor is the treatment of soaking ZPT consisting of 4 levels P0 (Without soaking ZPT), P1 (2 hours soaking ZPT), P2 (4 hours soaking ZPT), P3 (6 hours soaking ZPT), P4 (8 hours of soaking ZPT) , while the second factor is the volume of the growing media consisting of two kinds, V1 (80ml), V2 (200ml). Based on the results of analysis of variance study showed the combination treatment prolonged submersion tanamdan media volume ZPT no significant effect on the variable plant height, number of leaves, plant diameter, root length, root weight. Soaking ZPT on sugarcane crop seeds bud chip system with interval showed no real influence. the use of planting medium volume at the level V1 (80ml) and V2 (200) showed highly significant interaction. The use of volume media best planting with V2 (200ml) bud chip.
Test The Ability of Tricoderma harzianum and Bacillus subtilis As Control Agents of Wilted Ralstonia solanacearum Bacteria in Tobacco Plants (Nicotiana tobacum) Andriani Eko Prihatiningrum; Ahmad Khafids jalaluddin
Nabatia Vol 8 No 1 (2020): June
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.259 KB)

Abstract

This research aims to determine the interaction between Trichoderma harzianum and Bacillus subtilis as controlling bacteria wilting caused by Ralstonia solanacearum bacteria. This research was carried out in Laboratory of Microbiology and Green house at Science and Technology Universitas Muhammadiyah Sidoarjo and has been done on februari until March 2019. This research uses a completely randomized design (RAL) with 11 treatment and 3 replication to obtain 33 experimental units. The variables observed were the onset of symptoms of the dieses, indek of dieeses, stover wet weight planting. Data were ana- lyzed with variety of ANOVA followed by as 5% BNJ. The result of this research indicate that giving Trichoderma harzianum and Bacillus subtilis at the begining and simultane- ously can suppress pathogens than giving pathogens Ralstonia solanacearum first. This research showed the initial appearance of symptoms of dieses different very markedly bud the wet weight of bark and dry weight trimmed cropping showed different not real.
The The Effect Of Paitan (Thithonia Diversifolia L.) POC And Medium Of Fertilizer Plants ON The Growth and Production OF Pagoda Palm Plant (Brassica Nanirosa L.) Andriani Eko Prihatiningrum; Achmad Billyardi
Nabatia Vol 8 No 1 (2020): June
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.887 KB)

Abstract

The research was conducted in the village jiken tulangan sidoarjo. especially mustard pagoda still needed, opening up opportunities in the production of organic farming, which supports the public demand for mustard pagoda, pagoda mustard plants can respond to the growth and yield of mustard pagoda. as organic materials, this study aims to determine the effect of dose poc paitan and cow manure on the growth and yield of mustard pagoda. as well as to determine the dose poc paitan and cow manure is right at pagoda mustard growth in order to obtain the highest yield, The experiment was arranged in factorial design used was a randomized block dsign (RAK) first factor E0,E1,E2 and the second factor B1, B2. B3 repeated 3 replications and 9 treatments each test and the test continued HSD 5% The result of research show that dosing poc paitan and cow manure average – highest average on observation of plant height, leaf number, stem diameter, leaf area, fresh weight, dry weight and root showed the highest at treatment E1 = cow manure 100 gr (20 ton/ ha) and B= POC paitan 25ml/ltr.
Response Growth And Production Of Soybean (Glicine Max) On Composting Fertilizer (Azolla And Straw) And Additional Rhizobium Andriani Eko P; Didit Riancono
Nabatia Vol 6 No 1 (2018): June
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (229.873 KB)

Abstract

The need for soybeans is very high, but not balanced by soybean production. Recorded in 2014, the government should import kedelei of 67.28% of the national soybean demand. Therefore, sustainable agricultural system is needed to increase soybean production. The aim of this research is to know the combination of treatment of biological fertilizer (Rhizobium) and compost (azolla and straw). The experiment was conducted in Panjer village, Mojosari sub-district, Mojokerto, from April to July 2017. The research used randomized block design (RAK) factorial design with 2 factors. Factor 1 was rhizobium (without rhizobium (R0) and rhizobium (R1)) and factor 2 is the application of compost fertilizer (without the application of compost fertilizer (K0), azolla compost fertilizer (K1), the application of straw compost fertilizer (K2) and the composting of azolla compost and straw compost (K3)). From these two factors, 8 treatment combinations were repeated 3 times. Data were analyzed by multiform analysis followed by 5% duncan test. The results showed that the treatment combination was not significantly different, but the compost treatment showed that azolla compost was able to increase both vegetative and generative growth, whereas in biofertilizer was able to increase wet weight of root nodule but did not affect the increase of other observation parameters.
Co-Authors A'rasy Fahruddin A. Miftakhurrohmat Abdullah Fahmi Rabbani Abror, M. Achmad Baihaqi Achmad Billyardi Adawiyah, Ro’biatul Ade Eviyanti Agus Hayatal Falah Ahmad Khafids jalaluddin Aisyah Nurkhaliza Akhmad Mulyadi Alfrina Nabila Alhaq Alhaq, Alfrina Nabila Allysa Rowihatunnufus Hikmah Andajani, Erna Andika Wahyu Nugraha Assholikhah, Aisyah Dirra Belia Findurina Bilqis Sabrina A Budi, Setyo Budiandari, Rahma Utami Canda Kharisma Septabrina Danya Mozza Elshiva Denny Irawan Didit Riancono Emy Rosnawati Faiza Nur Aini Fajrian Moch Bintang Farabi, Ahmad Al Findurina, Belia fitriyah nur Fitriyan Nur Indahsari Hana Catur Wahyuni Hasan Ubaidillah Hilmi, Sectio Bachrul Ida Agustini Saidi Izza Anshori Jalaluddin, Ahmad Khafidh Kinanti, Kintan Sari Lely Ika Mariyati Li’aini, Arrohmatus Syafaqoh Lukman Hudi M Abror M. Amin Zakariyah mariah alqibtiyah Metatia Intan Mauliana Miftakhurrohmat, A. Miranda, Wiji Amelia Moch R Syarifuddin Fathony Muhammad Abror Muhammad Alif Muhammad Irfan Hakim Nabila Nurma Riski Nurkhaliza, Aisyah poppy diana Poppy Diana Sari Purwanti, Yanik Putri Karina Rachmadani Rabbani, Abdullah Fahmi Rachmadani, Putri Karina Rahmah Utami Budiandari Rima Azara Ro’biatul Adawiyah Sahara, Zhahlya Amaldha Saiful Arifin Sectio Bachrul Hilmi Septabrina, Canda Kharisma Shaza Dyah Ayuni Shazana Dyah Ayuni Siti Rahayu Sony Raharjo Sugeng Santoso Sutarman . Sutarman Sutarman Syahrorini, Syamsudduha Syamshudduha Syahrorini Syarifa Ramadhani Nurbaya Widayanti, Silvi Widyastuti Wiharyanti Nur Lailiyah Wiji Amelia Miranda