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International Journal of Applied Biology
Published by Universitas Hasanuddin
ISSN : 25802410     EISSN : 25802119     DOI : -
Core Subject : Science, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Automotive Engineering Energy Environmental Science
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
Arjuna Subject : -
Articles 168 Documents
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Bioactivity of Nypa fruticans Leaves as A Candidate for Anticancer Compounds Against MCF-7 Breast Cancer Cells: Bioactivity of Nypa fruticans Leaves as A Candidate for Anticancer Compounds Against MCF-7 Breast Cancer Cells Evy Noviana Noviana; Eva Johannes; Sjafaraenan
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.19091

Abstract

Breast cancer is the highest incidence of cancer for among women worldwide. The use of anticancer compounds from natural ingredients as cancer therapeutics is to reduce chemotherapy side effects. Indonesia has high biodiversity especially its pharmacologically beneficial such as Nipah. Nipah (Nypa fruticans W.) is a type of palm-shaped mangrove plant which widely found in watershed areas . This study aims to explores the bioactivity of ethanol extract from N. fruticans leaves against MCF-7 breast cancer cells. Screening for bioactive compounds was performed by Gas chromatography-mass spectrometry (GC-MS). Anticancer pre-screening was assessed by brine shrimp lethality (BSL) test and anticancer activity was assessed by 3-(4,5-Dimethylthiazol-2-cyl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. According of GC-MS analysis showed the presence of 54 phytochemical compounds. Some of them have bioactivity as anticancer including Sitosterol, Tocopherol, and Phytol. The BSLT method showed potent activity with LC50 was 84.25 µg/mL. Anticancer activity using MTT assays results the low IC50 value was 88.77 µg/mL that showed high anticancer activity by growth inhibition in MCF-7 breast cancer cells. This study delivers information that ethanol extract from N. fruticans leaves possesses bioactive compounds could be use as a candidate for anticancer compounds for cancer treatment.
Extraction,purification and characterization of amylase,protease,lipase of halophilic bacterial isolates from Bordi, India VIKAS JOSHI; anupama pathak; Supriya murkute; Bhoomi Das; zeenat khan
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.19118

Abstract

Extremely halophilic isolates were obtained from mangrove vegetation of west coast Bordi region, Maharashtra India. All six isolates were selected for further investigation on the basis of their potential of producing enzymes amylase, protease and lipases respectively. Production of enzyme from isolates was performed using basal medium with starch, skimmed milk and tributyrin. Ammonium sulphate salt precipitation and dialysis was performed to obtain partially purified crude samples of enzymes. Activity of crude enzymes was determined in unit/ml by using standard methods. Also the effect of pH and temperature on enzyme activity was also determined.
Synthesis of silver nanoparticles from Padina australis seawedd extract as bioreductor A. Nur Ainun
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.19227

Abstract

The synthesis of silver nanoparticles was carried out using silver nitrate solution as a precursor and Padina australis seaweed as a bioreductor. This study aims to obtain silver nanoparticles through the green synthesis method using Padina australis seaweed extract. The process of forming silver nanoparticles was carried out by adding Padina australis seaweed extract to 1mM AgNO3 solution. The nanoparticles were characterized using a UV-Vis spectrophotometer instrument. The results of the UV-Vis spectrum analysis showed that the best nanoparticle synthesis was shown in the 0.5% concentration extract with a duration of 30 minutes with a synthesis temperature of 70°C, with an absorbance peak at a wavelength of 445.00 nm. Based on visual observation, the extract turned reddish brown.
Indicator Film of Natural Coloring of Butterfly Pea (Clitoria ternatea L.) as Detection of Beef Damage : Indicator Film of Natural Coloring of Butterfly Pea (Clitoria ternatea L.) as Detection of Beef Damage Inanpi Hidayati Sumiasih
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.19241

Abstract

Beef is a food source with a high protein content that would be ideal for microbes to flourish. Microbes would reduce the quality of a product. Therefore, an indicator on smart packaging would be needed to detect the quality of a product. The indicator film in this study used butterfly pea flower extract which contains a natural coloring agent, anthocyanin. The objective of this study was to obtain the indicator film with the best concentration of PVA, chitosan, and butterfly pea flower extract as the natural dye, to study the response of indicator film color, pH, and thickness, to the beef pH and TVBN, and to calculate the total microbes as the determinant of the beef quality. The study consisted of three steps, namely, the extraction of butterfly pea flower, the making of indicator film, and the application of indicator film on beef packaging. The best indicator film was obtained with the formulation of PVA and chitosan of 20:80 with the addition of 5 ml of butterfly pea extract. The color change was from blue to yellowish-green with °hue of 137.81±19.31o. The thickness of indicator film in 48 hours of storage decreased from 0.171±0.042 to 0.136±0.043. The pH of beef increased after 8 hours of storage from 5.726±0.011 to 7.540±0.351. The TVBN of beef after 8 hours of storage had exceeded the threshold of 30.815±5,602 which indicates that it was not safe for consumption. The TPC of beef from the 8 hours of storage had exceeded the maximum number of 7.338±0.035 log CFU/g. Keyword: anthocyanin, butterfly pea extract, thickness, volatile bases
The Morphology Character of Japanese Taro (Colocasia esculenta var. Antiquorum) In Induction of Polyploidization Mutations In Vitro: A Case Study of Increased Concentration and Duration of Immersion of Colchicine Mutagens Sudirman Sudirman; Nirwansyah Amier; Ihsan Syawal Rahmat
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.19557

Abstract

Japanese taro is an alternative food commodity and has prospects as an export commodity, especially to Japan. Colchicine is a mutagen that can be used in polyploid mutations but can be toxic in plants. The purpose of this study was to determine the effect of various concentrations of colchicine and immersion time on the growth and morphological characters of Japanese taro plantlets in vitro. In this study, there were 2 focus factors of treatment. The first factor is colchicine concentration consisting of 0.0% (K0/control), 0.1% (K1), 0.2% (K2) and 0.3% (K3). the second factor is the immersion time consisting of 48 hours (T1), 72 hours (T2) and 96 hours (T3). The treatment of in vitro shoot immersion with 0.1%, 0.2% and 0.3% concentration of colchicine solution for 48 hours, 72 hours and 96 hours was significantly different from the control. Explant growth decreased every week and died at 6 MSI on colchicine treatment. The level of concentration and duration of immersion with different colchicine caused different color changes of the explants, the higher the concentration of colchicine and the duration of immersion, the color of the explants would quickly turn brown.
Wine Production from Banana (Musa sapientum) Using Yeast (Saccharomyces cerevisiae) Isolated from Grape (Vitis vinifera). Constance Chinyere Ezemba
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.19752

Abstract

This research was carried out to produce wine from banana (Musa sapientum) using yeast (Saccharomyces cerevisiae) isolated from grape (Vitis vinifera). The fermentation of the banana wine lasted for 21days. During fermentation, liquor of the fermenting “must” were removed every 48hours from the fermentor for analysis of pH, titratable acidity, specific gravity and reducing sugar using standard procedures.. The results from the experiment showed that Specific gravity of the wine was observed to reduce drastically as the fermentation progresses. The pH of the Banana wine during fermentation increased from 4.16- 4.22 while the titrable acidity of the Banana wine produced increased from 1.05-1.77. The alcohol content of the wine increased from 0.0 to 9%. The higher the fermentation temperature, the faster the conversion of sugars into alcohol and carbon dioxide by the yeast. The flavor and taste was appreciable. This study showed that acceptable wine can be produced from banana with the yeast Saccharomyces cerevisiae isolated from grape.
IIn Silico Testing On The Activity Of Flavonol In Sterculia foetida Leaf As Natural Anti Hyperlipidemia Compounds Nindrahayu Syam; Andi Ariyandy; Ika Yustisia; Sulfahri; Yusfina
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.19918

Abstract

This study aims to measure the biological activity of flavonols in the leaves of Sterculia foetida as an antihyperlipidemic drug. The chemical structure of flavonols contained in the leaves of Sterculia foetida was taken from the literature. The target protein used was 3-hydroxy 3-methylglutaryl coenzyme A reductase and the control was simvastatin. Water molecules have been removed with PyMol v2.5.2 Software. Docking between the target protein and flavonols was performed using PyRx-Python Prescription 0.8 Software. The results showed that flavonol compounds have greater potential for antihyperlipidemia compared to control compounds. The equivalent affinity of 3-hydroxy 3-methylglutaryl coenzyme A reductase for flavonols is -8.3, and the affinity of 3-hydroxy-3-methylglutaryl coenzyme A reductase for simvastatin is -7.9. Flavonol toxicity studies have shown that flavonols are not potentially carcinogenic and did not cause mutations. The absorption of flavonols in water was higher than that of the control compound.
The Biodiversity of Tunicate (Ascidiacea) in the waters of Badi Island, Pangkajene Regency of South Sulawesi Magdalena Litaay
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.20401

Abstract

Tunicates belongs to Urochordate that inhibit coral reefs ecosystem. The uniqueness of the tunic can be seen from the body shape like a tube with 2 siphons and the body is covered by a protective layer known as the tunic. The present study aims to observe marine tunicate at waters of Badi Island, Pangkajene Regency, South Sulawesi. This research using the Belt Transect method with a length of 20 m and a width of 4 m at a depth of 5 m with 4 station points according to the wind directions. All tunicate inside transect was noted and identified. Identification was based on morphological characteristics. The result shows that there were 18 species of tunicates present in the coral reefs ecosystem of Badi Island.
The Exchange Rate Of Bagan Rambo Fishermen In Barru District. Barru Regency, South Sulawesi Alpiani Alpiani
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.21335

Abstract

This study aims to analyze and examine the exchange rate of labour fishers on Bagan Rambo fishing gear in Barru district, Barru Regency. The fishers' exchange rate is used to measure the basic needs of fishers, which considers the income and expenditure of the households of the Bagan Rambo labourers. This type of research uses instrumental case studies. Analyze the poverty of fishers as an instrument for understanding their lives. The results showed that the exchange rate of fishers (NTN) of Bagan Rambo workers was lower than one hundred (88,49%), which means the Bagan Rambo labour fishers cannot meet their basic needs.
Tuna Fishery Resources Supply Chain (Thunnus sp) in Maumere District, Sikka Regency, East Nusa Tenggara Province Alpiani Alpiani
International Journal of Applied Biology Vol. 6 No. 1 (2022): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v6i1.21351

Abstract

The potential of Maritime Affairs and Fisheries owned by Indonesia becomes a massive opportunity in producing various types of commodities provided by natural resources such as the fishing fish, aquaculture, fishery products, and ecotourism. Based on this, it is necessary to have synergy between the government, the private sector and the community in managing marine fisheries resources that exist today. Of course, with the existing potential, it will become a challenge in its management that can guarantee the sustainability of natural resources and the improvement of the people's economy. This paper examines the opportunities and challenges of the fishing industry in Indonesia using the input and output data of the Directorate General of Maritime and Fisheries Resources Supervision, which are analyzed descriptively using tables and graphs and journal reviews relating to the study.

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