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International Journal of Applied Biology
Published by Universitas Hasanuddin
ISSN : 25802410     EISSN : 25802119     DOI : -
International Journal of Applied Biology is an international, peer reviewed journal, publishing high-quality research. International Journal of Applied Biology only publishes manuscripts in English. Please see the journal's Focus & Scope for information about its Scope and peer-review policy. International Journal of Applied Biology is available in print and online version.
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Articles 168 Documents
COLOR INDICATOR FILM FROM BUTTERFLY PEA (Clitoria ternatea L.) AS SMART PACKAGING IN BROILER CHICKEN MEAT Hermawan Seftiono; Della Ayu Pramesti; Inanpi Hidayati Sumiasih
International Journal of Applied Biology Vol. 5 No. (1) (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i(1).13431

Abstract

Broiler chicken is one of the poultry products that are very susceptible to damage due to exposure to microbes, temperature, and light. In these conditions, indicators are needed to play a role in informing related changes in the product quality. The butterfly pea can be used as an indicator because it contains anthocyanin which can change color due to the influence of pH. The objective of this study was to obtain the best indicator film formulation and to determine the film response in detecting the level of damage of the chicken meat through parameters of color change, thickness, pH, TPC, and TVBN. The methods in this research were the extraction of butterfly pea, making indicator films, and applying the film to chicken meat, and physical and chemical analysis of the product. Anthocyanin was applied to the indicator film with levels of PVA and chitosan 20:80 with the addition of 5 mL of dye. The change in color of the indicator could be seen at the 24-hour mark, which was a yellowish-green color. The pH value of broiler chicken meat had entered the post-rigor stage at 24 hours with a pH value of 6.595±0.049. The TVBN regression value showed that broiler chicken meat was not safe for consumption after being stored for 4 hours and 23 minutes of 40 mgN/100 g. The TPC test that has been performed in chicken meat showed that the chicken meat was not safe for consumption in storage between 0 to 8 hours at 5.598±0.068 log CFU/g to 8.322± 0.053 log CFU/g. Keywords: anthocyanin, discoloration, pH indicator, TVBN
The COVERAGE OF LLIN AMONG EXPECTANT MOTHERS IN NWANGELE, IMO STATE, NIGERIA CHIAGOZIEM OGAZIRILEM EMEROLE
International Journal of Applied Biology Vol. 5 No. (1) (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i(1).13538

Abstract

Background: long-lasting insecticide-treated nets (LLINs) is one of the major interventions for the control and elimination of malaria, especially among pregnant women. Aim: This study aimed to evaluate the knowledge, occurrence of malaria, utilisation and acquisition of long lasting insecticide treated net (LLINs) among pregnant women in Nwangele L.G.A. Imo State. Method: This was a cross-sectional study among women attending antenatal care in a primary health facility in Nwangele LGA, Imo State, Nigeria. A total of 150 women were interviewed using structured questionnaire to obtain information on their knowledge and the coverage of LLINs. Data analysis was done using SPSS version 20. Results: The mean age of the women was 29years. Most (81.3%) of the respondents had at least a secondary education. 92% used the LLINs at night time. Cost of acquiring these nets were free and in good condition (97.3%). Distance was listed as a hindrance to acquisition of nets (42.7%). Conclusion: Malaria is a very serious public health problem; prompt treatment alone cannot guarantee the achievement of the global eradication goal. All strategies must be strengthened and employed in preventing malaria occurrence if the goal is to be achieved.
Exploration Indigenous Bacillus Bumiaji-Malang Against Ralstonia solanacearum Causing Potato Bacterial Wilt Ni'matuzahroh Ni'matuzahroh
International Journal of Applied Biology Vol. 5 No. (1) (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i(1).14184

Abstract

Ralstonia solanacearum is a soil borne pathogen, which has a very wide host range and causes bacterial wilt. The use of biological agents, such as bacterial groups has been tried in several areas of potato plantations in Java. This study aims to obtain Bacillus isolates from the potato cropping area, Sumber Brantas, Bumiaji, Batu, which had the potential to suppress growth R. solanacearum. Bacillus was isolated from the soil rhizosphere of potato plants, while R. solanacearum was isolated from the base of potato stems showing bacterial wilt symptoms on tetrazolium chloride (TZC) selective medium. Bacillus spp. and R. solanacearum isolates were tested for hypersensitivity on the leaves of the KR-15 tobacco plant. Isolates that cause necrosis symptoms in tobacco leaves can be ascertained to be pathogenic. This study succeeded in obtaining 13 Bacillus spp. isolates with different colony morphologies. Three isolates of Bacillus spp. were selected from the 13 isolates obtained, based on their ability to suppress the growth of R. solanacearum and are expected to be potential as biological agents. Based on genetic analysis, the 3 bacterial isolates were identified as Bacillus mycoides and Bacillus weihenstephanensis.
THE EFFECTIVENESS OF ORGANIC FERTILIZER AND MICORIZA ARBUSCULA ON GROWTH AND PRODUCTIVITY GREEN EGGPLANT (SOLANUM MELONGENA L.) Diah Sudiarti
International Journal of Applied Biology Vol. 5 No. (1) (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Green eggplant (Solanum melongena L.) is a food plant grown for its fruit. The fruit of the eggplant depends on the fruit stalk and is a single true fruit, thick, soft, and juicy. This study was a completely randomized design with a three-treatment pattern. The first is a combination of organic fertilizer and arbuscular mycorrhizal fungi, the second is a positive control, namely using NPK fertilizer, the third is a negative control, namely without treatment. Each treatment was repeated three times and each repetition consisted of 3 plants. Plant growth parameters consist of plant height and number of leaves. Plant productivity parameters consist of the number of fruit, fruit wet weight, and dry weight. Based on the results of the study, it was obtained data that the combination of organic fertilizer and Arbuscular Mycorrhizal Fungi had better growth and productivity results compared to other treatments with an average plant height of 44.46; the average number of leaves 11.12; the average number of fruits was 5.51; mean fruit wet weight 106; and the average dry weight of fruit 84. The results of the Anova statistical test showed that the significance value of plant height and a number leaves was 0.000 (α <0.05), and the significance value for the number of fruits was 0.006 (α <0.05), while the significance value of weight wet and dry weight of fruit is 0.000 (α <0.05), this indicates that the combination of organic fertilizer and Arbuscular Mycorrhizal has a significant effect on the growth and productivity of green eggplant plants.
Antibiotic susceptibility pattern of Pseudomonas aeroginosa isolated from ready to eat food from selected street vending food locations in Ikpoba-Okha local government area of Edo State Shadrach Osalumhense Okhuebor; Osazee Izevbuwa
International Journal of Applied Biology Vol. 5 No. 2 (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i2.14440

Abstract

This study was conducted to analysis of the antibiotic susceptibility pattern of Pseudomonas aeroginosa isolated from ready-to-eat food in selected street food vendors in Ikpoba-okha LGA. The mean total viable plate counts (TVC) for Pseudomonas aeroginosa was ascertained with the spread plate methods using nutrient agar with results indicating a mean total viable count (TVC) ranging from 2.20 x 10⁵ to 1.05 x 10³. The highest counts of 2.20 x 10⁵ was obtained in food samples collected from SFL 5 while the lowest count of 1.05 x 10³ was obtained from SFL 1. The organism had a high percentage occurrence of 90% haven been isolated from most food samples (especially soups) from all street food vending location except SFL 3.The data obtained from the gram negative antibiotic susceptibility analysis showed that Pseudomonas aeroginosa was more sensitive to Ciprofloxacin (10µg) and Norfloxacin (10µg), sensitive to Gentamycin (10µg), Augmentin (30µg), Amikacin (30µg) and Ceftazidime (30µg) but showed resistance Cotrimoxazole (30µg), Ceftriazone (30µg) and Tetracycline (30µg). The results of this study indicates that most of the ready to eat food samples examined had high contamination of Pseudomonas aeroginosa and hence did not meet microbiological quality standards. Hence, it is recommended that a more close supervision of ready to eat food from street food vending locations in Ikpoba-okha should be carried out by relevant authorities.
Effectiveness Of Waste Management At The Hamlet Level In Mamasa Village Mamasa Regency Mildayati -
International Journal of Applied Biology Vol. 5 No. 2 (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i2.14581

Abstract

Population growth and changes in people's consumption patterns lead to an increase in the volume, types and characteristics of increasingly diverse waste. This increase in the amount of waste that is not followed by the repairment and improvement of waste management facilities and infrastructure has resulted in a complex waste problem. Therefore, one of the efforts that can be applied to overcome the waste problem is by taking a source approach. In this approach, waste will be handled (managed) at the upstream (source) before it reaches the landfill (downstream). Ways that can be done include sorting the waste and also the 3R (reduce, reuse and recycle) program. This study aims to determine the effectiveness of waste management at the hamlet level in Mamasa Village, Mamasa Regency. This study uses a descriptive qualitative approach with data reduction analysis. The results showed that the effectiveness of waste management was 37.5% and the effectiveness of waste reduction was 42%. The study concluded that the effectiveness of waste management at the hamlet level in Mamasa village was not very effective.
EFFECT OF NATURAL GROWTH REGULATORY SUBSTANCE (PGR) AND DIFFERENCES OF PLANTING MEDIA ON CHLOROPHIL CONTENT NUMBER OF VEGETABLESTOMATES AND AREA OF VEGETABLESTOMATES MICROGREENS BROCCOLI (Brassica oleracea L.) Achmad Roihan Roihan
International Journal of Applied Biology Vol. 5 No. 2 (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i2.14817

Abstract

Microgreens are plants from the group of vegetables or herbal plants that are harvested at the age of 7-21 days. As a horticultural crop, broccoli (Brassica oleracea L.) is a type of vegetable that belongs to the cabbage family (Brassicaceae). This study aims to determine the effect of natural ZPT and differences in growing media on chlorophyll content, number of stomata and stomata openings of broccoli microgreens. This study consists of 2 treatment factors. The first factor of the planting medium consists of husk charcoal (M1). Cocopeat (M2) and rockwool (M3). The second factor of natural ZPT consists of no ZPT (Z0), coconut water (Z1) and bean sprout extract (Z2). The combination treatment of rockwool growing media and natural zpt of bean sprout extract (M3Z2) gave the best results on parameters of chlorophyll a, b and total content, each of which was 14 days old. While the combination of treatment with cocopeat growing media and natural zpt of bean sprout extract (M1Z2) gave the best results on parameters of chlorophyll a, b and total content, each of which was 21 days after planting. The best parameters for the number of stomata and the area of stomata openings were found in the combination treatment of cocopeat growing media and natural zpt of coconut water (M1Z1), 15.00 and 115.13 μm2, respectively.
Grain yield evaluation of maize genotypes at different planting dates Santa Bahadur B.K.
International Journal of Applied Biology Vol. 5 No. 2 (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i2.14832

Abstract

The genotypic yield potential of maize is influenced by planting dates. A study was conducted in Rampur, Chitwan, Nepal, in 2013/14 to determine the best planting dates for maize genotypes. Every week from April, 2013 to March, 2014, four maize genotypes viz Hybrid RML-4/RML-17, RML-32/RML-17, ACROSS-9944/ACROSS-9942, and S99TLYQ-B were planted. The maximum grain production (5565 kg/ha) was recorded in August, followed by February (5266 kg/ha), June (4475 kg/ha), and July (4255 kg/ha). The lowest yield was obtained in November planting (2572 kg/ha) followed by December planting (3019 kg/ha). During August planting, the maximum grain yields were obtained in RML-4/RML-17 (7392 kg/ha) followed by RML-32/RML17 (6606 kg/ha) and ACROSS-9944/ACROSS-9942 (5004 kg/ha). The QPM genotype S99TLYQ-B produced the highest grain yield (4198 kg/ha) in February planting. Maize grown in the winter had a larger yield potentiality than maize grown in the rainy season. This study suggests that RML-4/RML-17, RML-32/RML17, and ACROSS-9944/ACROSS-9942 should be planted in August (winter season) and S99TLYQ-B in February (spring season) for maximizing production.
The potential distribution prediction of the invasive alien species Acacia decurrens Wild., in Indonesia Whisnu Febry Afrianto
International Journal of Applied Biology Vol. 5 No. 2 (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i2.17949

Abstract

Acacia deccurens Wild. has been reported as invasive alien species (IAS) in several areas of Indonesia. Climate change may impact IAS to be more invader. The study aimed was to develop a species distribution model of A. deccurens to depict the potential distribution under climate change in Indonesia. Biodiversity and Climate Change Virtual Laboratory (BCCVL) was used to examine a species distribution model (SDM) of A. decurrens in Indonesia based on climate variables and its naturalized distribution to predict the project distribution under current and future climate conditions. The data was collected from Global Biodiversity Information Facility (GBIF) to identify the species occurrences. The climate variables used in this study were temperature and precipitation layers based on WorldClim, current climate (1950-2000), 2.5 arcmin (~5km). The SDM of the Generalized Linear Model (GLM) was utilized to predict the response variable as a function of multiple predictor variables. We selected four IPCC Representative Concentration Pathways (RCP) 2.6, 4.5, 6.0, and 8.5 for 2050. The prediction of the distribution of A. deccurens in 2050 showed that it was likely to decrease in Indonesia (mostly found only in Sumatra and Sulawesi Island). Almost all climate variables used in this study were responsive to A. decurrens distribution, except B09 - mean temperature of the driest quarter. The ROC plot showed excellent values (0.99). The information of the potential distribution on IAS under current and future climate scenarios can be used for policymakers and stakeholders to manage and handle the invasion.
Ethnomedicinal Uses of Plants by Major Ethnic Tribes in Terai Districts of Nepal Mahamad Sayab Miya; Sachin Timilsina; Asmit Neupane
International Journal of Applied Biology Vol. 5 No. 2 (2021): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v5i2.18169

Abstract

Indigenous communities and tribes of Nepal have been using medicinal plants for the treatment of several diseases or ailments since their origin. Various studies were conducted in the Terai region of Nepal regarding the pharmacological, ethnobotanical, and medicinal importance of plant species. This study aims to compile information on ethnomedicinal uses of plants by major ethnic groups in the Terai districts of Nepal. A total of 35 published documents based on ethnomedicinal uses of several medicinal plants in Terai of Nepal till 2020 A.D. were accessed for the study from online portals like Research Gate, Scopus, and Google Scholar. A total of 300 plant species from 98 families were documented which are used for the treatment of 120 diseases or ailments by the 8 ethnic groups of 6 terai districts. Leaf of plants is found to be used for the treatment of maximum number of diseases (72). Plants from families like Fabaceae, Malvaceae, Poaceae, etc. were used for the treatement of diseases like Diabetes, jaundice, syphilis, gastritis and other wounds, fractures along mental disorders. Indigenous knowledge and ethnomedicinal importance of different plant species must be explored, documented, and passed through different generations which could widen the scope of modern herbal medicine science.

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