SUTARNO SUTARNO
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sebelas Maret. Jl. Ir. Sutami 36A, Surakarta 57126, Central Java, Indonesia

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Sustainability transitions and potential naturalization risk in the trade and cultivation of Selaginella kraussiana in tropical Indonesia AHMAD DWI SETYAWAN; SUTARNO SUTARNO; SUGIYARTO SUGIYARTO; SUNARTO SUNARTO; DIANTI DIANTI
Asian Journal of Agriculture Vol. 9 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g090259

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Abstract. Setyawan AD, Sutarno, Sugiyarto, Sunarto, Dianti. 2025. Sustainability transitions and potential naturalization risk in the trade and cultivation of Selaginella kraussiana in tropical Indonesia. Asian J Agric 9: 925-945. Selaginella kraussiana has recently become popular as a tropical ornamental plant in Indonesia because of its attractive morphology, rapid vegetative growth, and suitability for humid shaded environments. However, information regarding the multidimensional sustainability of its cultivation and trade systems remains limited, particularly concerning ecological resilience, socio-economic performance, and potential naturalization risk associated with ornamental dissemination. This study evaluated the sustainability of S. kraussiana cultivation and trade systems in Central Java, Indonesia, using a RAPFISH-based multidimensional framework integrated with SWOT-TOWS analysis. Primary data were collected through semi-structured interviews, field observations, and environmental assessments involving 40 ornamental plant farmers and traders. Sustainability evaluation included ecological, economic, social, technological, and institutional dimensions. The ecological dimension achieved the highest sustainability index (83.93%), indicating highly sustainable cultivation conditions supported by humid microclimates and vegetative propagation systems. Economic sustainability was categorized as moderately sustainable (58.29%), whereas social sustainability remained low sustainable (36.70%). Technological and institutional dimensions showed unsustainable conditions (18.29%) due to limited innovation, weak institutional support, and low organizational integration. Monte Carlo validation confirmed strong RAPFISH ordination reliability with stress values below 0.25 and coefficients of determination (R²) above 0.94. The study indicates that increasing ornamental trade and repeated movement of vegetative planting materials may elevate propagule pressure and warrant precautionary ecological monitoring under favorable humid tropical environments. These findings highlight the urgent need for integrating ecological precaution, technological adaptation, institutional strengthening, and biodiversity conservation into sustainable tropical ornamental horticulture systems while minimizing potential ecological risks associated with ornamental dissemination.
Molecular characterization of Plant Growth Promoting Rhizobacteria using 16S rRNA sequences in the organic rice field of Sukorejo Village, Central Java, Indonesia SLAMET SANTOSA; SUTARNO SUTARNO; EDI PURWANTO; SURANTO SURANTO; SAJIDAN SAJIDAN
Biodiversitas Journal of Biological Diversity Vol. 19 No. 6 (2018)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d190623

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Santosa S, Sutarno, Purwanto E, Suranto, Sajidan. 2018. Molecular characterization of Plant Growth Promoting Rhizobacteria using 16S rRNA sequences in the organic rice field of Sukorejo Village, Central Java, Indonesia. Biodiversitas 19: 2157-2162. Plant Growth Promoting Rhizobacteria (PGPR) are rhizosphere bacteria that can be utilized to increase plant growth and suppress plant diseases. PGPR concentrate on the concept of sustainable agriculture due to their role as biostimulant by synthesizing and regulating the concentration of various phytohormones and as biofertilizers. It is important to determine the original PGPR found in the roots of rice plants. This study aimed to identify PGPR based on 16S rRNA sequences. The bacterial strain was isolated from the rhizospheric soil of IR 64 organic and inorganic rice fields in the region of Sragen District, Central Java, Indonesia. The bacterial isolates were grown on the Luria Bertani (LB) Agar Medium. There were 10 colonies obtained with different morphological variations, nine of those were from the organic rice field. Molecular characterization using 16R rRNA sequences suggested the identity the nine isolates were of Pseudomonas aeruginosa strain RI-98-1, Stenotrophomonas maltophilia strain S431, Bacillus subtilis strain CEB2, Bacillus cereus strain ATCC 14579 clone EA195, S. maltophilia strain 5517, Exiguobacterium acetylicum strain SSA-3, Serratia nematodiphila strain HC4, Bacillus cereus strain ANP221, and Acinetobacter junii strain M. pstv. 21.4 Pseudomonas and Bassilus produce phytohormones (auxins) and convert inorganic phosphate to organic and Stenotropomonas can increase the growth of Acinetobacter and Exigobacterium as phosphate solvents in the soil.
Azolla microphylla and Pseudomonas aeruginosa for bioremediation of bioethanol wastewater Khoirul Annisa; SUTARNO SUTARNO; SLAMET SANTOSA
Biodiversitas Journal of Biological Diversity Vol. 22 No. 4 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220425

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Abstract. Annisa K, Sutarno, Santosa S. 2021. Azolla microphylla and Pseudomonas aeruginosa for bioremediation of bioethanol wastewater. Biodiversitas 22: 1799-1805. Bioremediation is the right choice in wastewater treatment since it requires has low cost but works optimally. Azolla microphylla and Pseudomonas aeruginosa are considered to have the ability to optimize waste degradation. The aim of this study was to determine the best treatment for reducing pollutants in bioethanol waste, namely by using A. microphylla and P. aeruginosa. The twelve treatments in this experimental research included variations in the A. microphylla biomass and variations of biomass combinations with the density of the P. aeruginosa. Research method used was completely randomized design with three replications, and all 36 samples were taken on the seventh day. Research data collected were the quality parameters of bioethanol wastewater before and after the treatment. The results showed that TSS, BOD, COD were decreased during seven days of treatment, whereas pH and H2S levels were increased. The combination of 0.2 kg A. microphylla and 1010 cfu/mL P. aeruginosa showed the best result in reducing pollutants. This study showed that A. microphylla and P. aeruginosa could be used to reduce pollutants in bioethanol wastewater. Results of the research are expected to become an alternative solution to the bioethanol wastewater problem.
Projecting expansion range of Selaginella zollingeriana in the Indonesian archipelago under future climate condition AHMAD DWI SETYAWAN; JATNA SUPRIATNA; NISYAWATI NISYAWATI; ILYAS NURSAMSI; SUTARNO SUTARNO; SUGIYARTO SUGIYARTO; SUNARTO SUNARTO; PRAKASH PRADAN; SUGENG BUDIHARTA; ARI PITOYO; SAPTA SUHARDONO; PRABANG SETYONO; MUHAMMAD INDRAWAN
Biodiversitas Journal of Biological Diversity Vol. 22 No. 4 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220458

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Abstract. Setyawan AD, Supriatna J, Nisyawati, Nursamsi I, Sutarno, Sugiyarto, Sunarto, Pradan P, Budiharta S, Pitoyo A, Suhardono S, Setyono P, Indrawan M. 2021. Projecting expansion range of Selaginella zollingeriana in the Indonesian archipelago under future climate conditions. Biodiversitas 22: 2088-2103. The expansion of plant species to outside areas of its original localities has attracted great interest in theoretical ecology. The scientific curiosity of such phenomenon is even deeper when the geographical expansion is confined by natural boundaries and affected by environmental changes, including climates. This study aimed to predict the current suitable habitat niche of Selaginella zollingeriana Spring, a species with original distribution in Java Island, and to project its potential suitable niche in the Indonesian archipelago accounting for future climate conditions. In doing so, we applied the Ecological Niche Modelings (ENMs) using MaxEnt algorithm by employing 30 presence data of S. zollingeriana and twelve enviro-climatic variables. The model predicted around 17.22% (22,095 km2) of the Java Island area is potentially suitable for current habitat niche of S. zollingeriana, consisting of 10.93% (14,028 km2), 4.75% (6,097 km2), and 1.54% (1,970 km2) of low, medium, and high suitability areas, respectively. Under future scenarios, the model predicted the possibility of species expansion into the other four big islands (i.e., Sumatera, Borneo, Sulawesi, and Papua). Nonetheless, the model also predicted a declining trend of the availability of suitable niches wherein from 2030 to 2080 the modeled niche declined about 58% and 59% under the most optimistic and most pessimistic climate change projections, respectively. While this study provides a primary example in predicting species expansion in tropical archipelago, similar studies in a range of contexts (e.g., species, region) are recommended to add more evidence to strengthen the theoretical ground of expansion ecology under climate changes.
Anticipated climate changes reveal shifting in habitat suitability of high-altitude selaginellas in Java, Indonesia AHMAD DWI SETYAWAN; Jatna Supriatna; Nisyawati; Ilyas Nursamsi; SUTARNO SUTARNO; SUGIYARTO SUGIYARTO; SUNARTO SUNARTO; Prakash Pradan; SUGENG BUDIHARTA; ARI PITOYO; SAPTA SUHARDONO; PRABANG SETYONO; MUHAMMAD INDRAWAN
Biodiversitas Journal of Biological Diversity Vol. 21 No. 11 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d211157

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Abstract. Setyawan AD, Supriatna J, Nisyawati, Nursamsi I, Sutarno, Sugiyarto, Sunarto, Pradan P, Budiharta S, Pitoyo A, Suhardono S, Setyono P, Indrawan M. 2020. Anticipated climate changes reveal shifting in habitat suitability of high-altitude selaginellas in Java, Indonesia. Biodiversitas 21: 5482-5497. High-altitude ecosystems with humid and cool climate are the preferred habitat for some Selaginella species (selaginellas). Such habitats are available in Java, Indonesia, which also has fertile soils with rich mineral contents resulted from volcanic activities. However, the high-altitude ecosystems in Java are threatened by various anthropogenic activities as well as changes in climate conditions, potentially affecting some Selaginella species. This study aimed to investigate the shift in suitable habitat of four species of high-altitude Selaginella spp. (Selaginella involvens, S. opaca, S. ornata, and S. remotifolia) in Java Island under current and future climate conditions predicted by several representative greenhouse gas concentration pathways. Presence data of Selaginella localities were collected from field survey between 2007 and 2014 across the island, as well as occurrence points from the Global Biodiversity Information Facility database. A total of 1,721 occurrence points data along with environmental and climate data were used to develop species distribution models using MaxEnt. Future habitat distributions were projected under four climate scenarios to see the shift in suitable habitat and altitudinal ranges. The results showed that the distribution of the four high-altitude Selaginella species are strongly influenced by altitude, annual average temperature, and annual rainfall. In the present time, 37.32% (48,974 km2) of the area of Java has been predicted to be suitable for high-altitude Selaginella. Under the optimistic climate scenario (RCP 2.6), the highly suitable area will likely to decrease by almost 35% in the year 2080, whereas the medium and low suitable areas will reduce by about 37.2% and 18.3%, respectively. Under the pessimistic scenario (RCP 8.5), about 21.2% of low suitable areas will be lost in 2080, whereas the medium and highly suitable areas are predicted to decrease by around 38.1% and 33.4%, respectively. Under the pessimistic scenario, there will be upward shift by 51.1 m in the year 2030 from the current’s mean altitude and will shift by almost 150 m in the year 2080. The maximum altitude of predicted suitable habitat is also predicted to increase to reach almost 3500 m asl in the year 2080. The results of this study imply that habitat shift of four high-altitude Selaginella species varies depending on the scenario, but in all cases, the losses are greater than gains.
Local knowledge and the utilization of non-medicinal plants in home garden by the people of Donorejo Village in the Menoreh Karst Area, Purworejo, Central Java, Indonesia AINI MAR'ATUSH SHOLEKHA; INGGRID TRIFENA YULIA; ZAHRA HANUN; IYUALEVI GARNIS PERWITASARI; AGUSTINA PUTRI CAHYANINGSIH; SUNARTO SUNARTO; SUTARNO SUTARNO; INOCENCIO E. Jr. BUOT; AHMAD DWI SETYAWAN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 1 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240173

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Abstract. Sholekha AM, Yulia IT, Hanun Z, Perwitasari IG, Cahyaningsih AP, Sunarto, Sutarno, Sugiyarto, Buot Jr IE, Setyawan AD. 2023. Local knowledge and the utilization of non-medicinal plants in home garden by the people of Donorejo Village in the Menoreh Karst Area, Purworejo, Central Java, Indonesia. Biodiversitas 24: 645-657. Donorejo Village, in Purworejo District, Central Java, Indonesia, is one of the villages in the Menoreh Karst Mountains that uses non-medicinal plants. Donorejo villagers typically own vast plots of land that might be used for a wide range of productive purposes if planted with suitable vegetation. This study aims to investigate the knowledge of local people in the Menoreh Karst Mountain Area, Donorejo Village regarding the use of non-medicinal plants for daily life and document the use of plants by locals that can be useful to prevent delays in the transmission of local knowledge to future generations. Interviews and surveys conducted in the field using a purposive sampling strategy provided the bulk of the study's information. The age range of the 48 informants was from 25 to 91 years old, and the vast majority of them had only completed elementary school. A total of 119 species of non-medicinal plants were identified, representing 62 families and 12 different purposes, based on the results of the inventory of plants indicated by respondents and field observations. These plants were utilized as ornamental plants (48 species), food plants (44 species), cooking spices (16 species), animal feed (13 species), firewood (6 species), building materials (6 species), household items (2 species), and funeral ritual, prayer beeds, food wrapper, fence, fire starter (1 species). Based on these findings, it is clear that the residents of Donorejo Village continue to employ various non-medicinal plants in their gardens for various purposes.
Estimating carbon storage using remote sensing in the Selo Resort forest area of Mount Merbabu National Park, Central Java, Indonesia MUHAMMAD NUR SULTON; NUR ROHMAYANI ANGGELIKA PUTRI; RACHEL SANISCARA NUGRAHENI; RATIH NUR AFIFAH; RIZKY NUR FADILAH; MUHAMMAD INDRAWAN; LIA KUSUMANINGRUM; SUNARTO SUNARTO; SUTARNO SUTARNO; SUGIYARTO SUGIYARTO; PRAKASH PRADHAN; AHMAD DWI SETYAWAN
Biodiversitas Journal of Biological Diversity Vol. 24 No. 11 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d241149

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Abstract. Sulton MN, Putri NRA, Nugraheni RS, Afifah RN, Fadilah RN, Indrawan M, Kusumaningrum L, Sutarno, Sunarto, Sugiyarto, Pradhan P, Setyawan AD. 2023. Estimating carbon storage using remote sensing in the Selo Resort forest area of Mount Merbabu National Park, Central Java, Indonesia. Biodiversitas 24: 6264-6270. This study is of paramount importance for long-term climate change mitigation efforts, as it aims to estimate carbon storage and assess land cover density in Selo Resort forest area of Mount Merbabu National Park (MMbNP), Central Java, Indonesia, utilizing remote sensing techniques. Sentinel 2A satellite imagery, acquired on May 28, 2023, serves as the foundational dataset for this research. Employing ArcGIS 10.5, the satellite imagery was processed to derive the Normalized Difference Vegetation Index (NDVI), a critical parameter in this analysis. The NDVI values were subsequently employed to categorize land cover density, leading to the classification of land into five distinct classes: non-vegetation, shrubs, low density, medium density, and high density. This classification process enabled the determination of land cover density. The calculated values for each density class, namely low, medium, and high-density land cover, revealed substantial carbon storage potentials: 12,357.99 tons C, 92,871.04 tons C, and 73,542.06 tons C, respectively. Moreover, the computed areas for low, medium, and high-density cover were 202.59 ha, 364.93 ha, and 348.26 ha, respectively. This study underscores the need for ongoing monitoring to detect changes in land cover and to implement afforestation initiatives in areas with low-density vegetation. Such efforts are crucial for the preservation of land cover and the associated carbon storage benefits, thereby contributing to broader climate change mitigation strategies.
Putative occurrence of amentoflavone across Selaginella species inferred from HPLC Retention Time analysis AHMAD DWI SETYAWAN; TATIK CHIKMAWATI; MIFTAHUDIN MIFTAHUDIN; PUSPA DEWI N. LOTULUNG; SUTARNO SUTARNO; SUGIYARTO SUGIYARTO; SUNARTO SUNARTO
Biodiversitas Journal of Biological Diversity Vol. 26 No. 12 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261246

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Abstract. Setyawan AD, Chikmawati T, Miftahudin, Lotulung PDN, Sutarno, Sugiyarto, Sunarto. 2025. Putative occurrence of amentoflavone across Selaginella species inferred from HPLC Retention Time analysis. Biodiversitas 26: 6490-6501. Amentoflavone is a prominent biflavonoid frequently reported in the genus Selaginella and widely studied for its diverse biological activities. However, information on its distribution across species remains fragmented and largely restricted to isolated reports on individual taxa. This study aims to provide a comparative, compound-centric assessment of the putative occurrence of amentoflavone across multiple Selaginella species using a standardized Retention Time (RT)-based HPLC approach. Plant materials representing 20 samples from 17 Selaginella species were analyzed under uniform extraction and chromatographic conditions. An authentic amentoflavone reference standard was used to establish an external RT benchmark, and chromatograms recorded at 270 nm were qualitatively evaluated for RT correspondence within a predefined RT screening window centered on the reference peak. Occurrence was assessed using a presence–absence framework without quantitative estimation or multivariate analysis. Putative RT correspondence consistent with amentoflavone was detected in 9 of the 17 analyzed species, while several taxa consistently lacked detectable RT-matched peaks. The observed RT ranges among positive species were narrowly clustered, indicating stable chromatographic behavior of the detected signal across different plant matrices. Intraspecific comparisons revealed contrasting presence–absence outcomes among populations of the same species, highlighting population-level variability in detectable compound occurrence. The results demonstrate that putative amentoflavone occurrence in Selaginella is species-specific and not universally distributed across the genus. This study underscores the value of RT-based HPLC as an exploratory, hypothesis-generating screening tool for mapping compound occurrence across diverse taxa, while explicitly acknowledging its limitations in structural confirmation and sensitivity. By providing a qualitative baseline, this screening-based framework offers an initial biotechnological entry point for prioritizing Selaginella taxa for targeted metabolite validation and subsequent investigation using structure-confirming analytical techniques.
Taxonomic reassessment of the Selaginella flora of Java, Indonesia AHMAD DWI SETYAWAN; TATIK CHIKMAWATI; MIFTAHUDIN MIFTAHUDIN; SUTARNO SUTARNO; SUGIYARTO SUGIYARTO; SUNARTO SUNARTO
Biodiversitas Journal of Biological Diversity Vol. 27 No. 2 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270239

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Abstract. Setyawan AD, Chikmawati T, Miftahudin, Sutarno, Sugiyarto, Sunarto. 2026. Taxonomic reassessment of the Selaginella flora of Java, Indonesia. Biodiversitas 27 (2): d270239. https://doi.org/10.13057/biodiv/d270239. Selaginella Beauv. is one of the most taxonomically complex lycophyte genera in tropical Asia, and species delimitation in Java has long been complicated by extensive morphological variation, nomenclatural inconsistency, and contrasting taxonomic interpretations. This study provides a comprehensive floristic and taxonomic reassessment of Selaginella in Java based on 1,962 occurrence records from field surveys and herbarium collections. Species diversity, distribution patterns, and taxonomic relationships were evaluated using comparative morphology, ecological evidence, herbarium examination, and contemporary nomenclatural standardization. A total of 21 species are recognized, including 19 native taxa and two introduced or naturalized species (S. kraussiana and S. uncinata). Updated diagnostic descriptions and identification keys are provided, and several problematic taxa are reassessed. Selaginella ascendens and S. springiana are interpreted within the S. intermedia complex, whereas S. caudata and S. caulescens are treated within the S. plana and S. involvens complexes, respectively. Several vegetative characters traditionally used for species delimitation, including branch flattening, foliage density, and leaf overlap, were found to be highly variable and environmentally responsive, whereas stem pubescence, aristate leaf apices, and certain growth-form characteristics remain more reliable for identification. Floristic comparisons indicate strong affinities between the Javan Selaginella flora and other regions of Sundaland, particularly Sumatra and Peninsular Malaysia, together with secondary connections to Wallacea and the broader tropical and subtropical regions of Asia. The study provides an updated checklist, identification framework, and taxonomic baseline for future ecological, biogeographical, and evolutionary research on Asian Selaginella.
Genetic diversity and molecular differentiation among Javanese Selaginella revealed by SSR and ISSR markers AHMAD DWI SETYAWAN; JATNA SUPRIATNA; D. DARNAEDI; ROKHMATULOH ROKHMATULOH; SUTARNO SUTARNO; SUGIYARTO SUGIYARTO; SUNARTO SUNARTO; YULITA KUSUMADEWI
Biodiversitas Journal of Biological Diversity Vol. 27 No. 5 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270520

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Abstract. Setyawan AD, Supriatna J, Darnaedi D, Rokhmatuloh, Sutarno, Sugiyarto, Sunarto, Kusumadewi Y. 2026. Genetic diversity and molecular differentiation among Javanese Selaginella revealed by SSR and ISSR markers. Biodiversitas 27 (5): d270520. https://doi.org/10.13057/biodiv/d270520. Selaginella is a highly diverse lycophyte genus in tropical Asia, yet molecular information for Indonesian taxa remains limited, particularly for evaluating species differentiation across geographically separated populations. This study assessed genetic diversity and molecular relationships among 32 Selaginella accessions representing eight recognized species and three unidentified taxonomic entities, collected mainly from Java, Indonesia, with additional materials from Bali, Lombok, and Papua. Genetic variation was analyzed using four SSR primers and five ISSR primers, which together generated 122 scorable loci, all of them polymorphic. SSR markers produced 43 loci, whereas ISSR markers generated 79 loci and contributed higher discriminatory power through greater polymorphism information content, resolving power, and marker index. Genetic similarity coefficients ranged from 0.42 to 0.93, indicating moderate to high molecular affinity among accessions. Both UPGMA clustering and principal component analysis consistently separated the major taxa, although widespread species such as S. plana and S. involvens showed moderate internal dispersion across accessions from different localities. AMOVA revealed that 80.1% of molecular variation occurred within species and 19.9% among species, with significant species-level differentiation (PhiPT = 0.199; P = 0.001). Nei's genetic distance revealed moderate molecular affinity among most Javanese taxa, whereas the Lombok and Papuan accessions occupied more divergent molecular positions. Geographic origin had little influence on the overall molecular structure, suggesting that species identity was the primary determinant of genetic differentiation. These findings demonstrate that combining SSR and ISSR markers provides a reliable molecular framework for refining taxonomy and supporting future conservation efforts for tropical Selaginella in Indonesia.