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All Journal Buletin Dharmas Andalas Biodiversitas Journal of Biological Diversity
Ashrifurrahman Ashrifurrahman
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Andalas. Jl. Raya Unand, Limau Manis, Padang 25163, West Sumatra, Indonesia
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Electrical & Electronics Engineering Library & Information Science

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Implementasi Praktikum Biologi Berbasis Teknologi untuk Peningkatan Kompetensi Siswa dan Guru di SMU N 15 Padang Rita Maliza; Muhammad Idris; Ashrifurrahman Ashrifurrahman; Arthauly Yopita Sinurat; M.Nazri Janra; Nofrita Nofrita; Muhammad Syukri Fadil; Robby Jannatan; Mansyurdin Mansyurdin; Wilson Novarino; Kurniadi Ilham; Putra Santoso; Henny Herwina; Bramadi Arya; Tasya Putri Pratama Elisa; Reziq Marchellino Irwan
Buletin Dharmas Andalas Vol. 3 No. 1 (2026): Buletin Dharmas Andalas
Publisher : Departemen Budidaya Tanaman Perkebunan, Fakultas Pertanian, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/bda.v3i1.60

Abstract

This community service activity was conducted on October 27, 2025, at SMA Negeri 15 Padang, with the aim of enhancing the competencies of teachers and students in biotechnology through the integration of modern technology as part of the digital transformation of education. The methods employed included direct training and mentoring, supported by a guidebook entitled “Basic Biology and Biotechnology: From Microscope to DNA.” The activity was organized into several thematic groups, namely plant and animal tissue histology, animal anatomy, plant culture, and DNA isolation. The results demonstrated a significant improvement in participants’ abilities to operate basic biotechnology tools, understand applied biotechnology concepts, and develop innovative practicum activities aligned with the Merdeka Curriculum. In addition, this activity strengthened collaboration among schools, universities, and media in disseminating science learning innovations. In conclusion, the implementation of technology-based biology practicum is effective in improving participants’ competencies and scientific literacy and has strong potential for sustainable development through continued mentoring and the development of digital modules.
Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: Molecular forensic application ASHRIFURRAHMAN ASHRIFURRAHMAN; SARUEDI SIMAMORA; RUSDIYAN RITONGA; WILSON NOVARINO; DJONG HON TJONG; RIZALDI RIZALDI; SYAIFULLAH SYAIFULLAH; DEWI IMELDA ROESMA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 4 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230410

Abstract

Abstract. Ashrifurrahman, Simamora S, Ritonga R, Novarino W, Tjong DH, Rizaldi, Syaifullah, Roesma DI. 2022. Sumatran tiger identification and phylogenetic analysis based on the CO1 gene: molecular forensic application. Biodiversitas 23: 1788-1794. Wild animal hunting, especially in the Sumatran tiger (Panthera tigris sumatrae), has been caused the population decline. Regulation and law enforcement have been implemented even though it does not affect optimal because of the trickery of poachers and illegal traders. Sometimes, the evidence of P. t. sumatrae derivative products, for example, bones, nails, skins, hair, and other body parts, cannot be properly identified to raise the cases. However, genetic markers, such as the CO1 gene, have successfully identified illegal trafficking samples. This study used 20 samples, consisting of seven samples (four preserved hairs, two claws, one bone) that were suspected of P. t. sumatrae collected from illegal wildlife trade cases in West Sumatra, Indonesia. Other thirteen samples were twelve blood and one hair of P. t. sumatrae samples were collected from the Dharmasraya Sumatran Tiger Rehabilitation Center (PR-HSD). All samples were isolated, Polymerase Chain Reaction (PCR), sequenced, and 999 base pairs (bp) of the CO1 gene sequences were analyzed. In addition, National Center for Biotechnology Information (NCBI) data sequences including two P. t. sumatrae sequences, four P. t. altaica sequences, one P. t. amoyensis sequence, one P. t. corbetti sequence, three P. pardus sequences, and one Felis catus sequence were collected for comparison and supporting data. The result confirmed that all samples in this study were P. t. sumatrae. We determined those depending on similarity value which was 99.60%-99.70% with P. tigris reference sequence (NC_010642.1) and 99.90%-100% with P. t. sumatrae (JF357969.1). Phylogenetic analysis supported species identification with average intraspecies sequence divergence was 0 to 0.4% and presented the monophyletic group. This study was the first and most recent report to use seized samples to identify P. t. sumatrae based on the CO1 gene in West Sumatra, Indonesia.
Mitochondrial COI gene-based phylogenetic and haplotype analysis of Manouria emys from Sumatra, Indonesia ASHRIFURRAHMAN ASHRIFURRAHMAN; SYAIFULLAH SYAIFULLAH; DEWI IMELDA ROESMA; DJONG HON TJONG; INDRI LESTARI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 12 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261224

Abstract

Abstract. Ashrifurrahman, Syaifullah, Roesma DI, Tjong DH, Lestari I. 2025. Mitochondrial COI gene-based phylogenetic and haplotype analysis of Manouria emys from Sumatra, Indonesia. Biodiversitas 26: 6224-6231. Genetic and phylogenetic analyses are essential for understanding the evolutionary relationships and genetic variation of the critically endangered Manouria emys, particularly in Indonesia, where molecular data remain limited. This study aimed to determine the phylogenetic placement and haplotype diversity of M. emys from Sumatra, Indonesia representing the first COI record from this region. A single sample collected from West Sumatra was analyzed through DNA extraction, PCR amplification of the COI (Cytochrome Oxidase Subunit I) gene, and sequencing. An 886 bp COI gene fragment was confirmed as M. emys through sequence similarity analysis and subsequently aligned with 29 global reference sequences for phylogenetic and haplotype analyses. Phylogenetic analysis using the Maximum Likelihood method showed that the Sumatran sample clustered within the M. emys emys clade, together with sequences from Borneo and the Taipei Zoo. Three subclades were observed within M. emys, corresponding to M. emys emys, M. emys phayrei, and one distinct genetic lineage of unconfirmed subspecies status. The analysis showed low genetic divergence within each subspecies but relatively high differentiation between M. emys and the outgroup. Haplotype analysis identified three main haplogroups, with the Sumatran sample showing close genetic affinity to M. emys emys from Borneo. The presence of multiple M. emys emys haplotypes reported from India may reflect population movement or human mediated translocation, highlighting the need for broader regional studies. This study provides the first molecular evidence of M. emys from Sumatra, offering a valuable genetic reference for future research, conservation management, and monitoring of wildlife trade involving this critically endangered tortoise.
Co-Authors . Mansyurdin Arthauly Yopita Sinurat Bramadi Arya DEWI IMELDA ROESMA DEWI IMELDA ROESMA Djong Hon Tjong Henny Herwina INDRI LESTARI Kurniadi Ilham M.Nazri Janra Maliza, Rita Muhammad Idris Muhammad Syukri Fadil Nofrita Nofrita Putra Santoso Reziq Marchellino Irwan RIZALDI RIZALDI Robby Jannatan RUSDIYAN RITONGA SARUEDI SIMAMORA SYAIFULLAH SYAIFULLAH SYAIFULLAH SYAIFULLAH Tasya Putri Pratama Elisa Wilson Novarino