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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia Jurnal Ekologi Kesehatan AL KAUNIYAH Elkawnie Journal of Science and Applicative Technology Civil Engineering Journal Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Agro Bali: Agricultural Journal Journal of Multidisciplinary Applied Natural Science
Muhammad Asril
Department Of Biology, Institut Teknologi Sumatera, Bandar Lampung-35365 (Indonesia)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Physics Public Health

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Chemical Composition, Antioxidant, Antibacterial, Antibiofilm, and Cytotoxic Activities of Robusta Coffee Extract (Coffea canephora) Erma Suryanti; Dwi Retnowati; Muhammad Eka Prastya; Novita Ariani; Indri Yati; Vera Permatasari; Tjandrawati Mozef; Indah D. Dewijanti; Apriza Yuswan; Muhammad Asril; Elisa Nurma Riana; Irmanida Batubara
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.632-642

Abstract

Coffee extracts are a rich source of potential compounds with numerous biological activities. This study aimed to investigate the potential of chemical compounds derived from robusta coffee extract (Coffea canephora) and its dominant compound, caffeine, to scavenge free radicals, inhibit bacterial growth and biofilm formation, and cytotoxic properties in human breast adenocarcinoma cell line. Chemical constituents of coffee extract were analysed quantitively for total phenolic, flavonoid, and alkaloid contents, along with Liquid Chromatography Quadrupole-Mass Spectrometry (LC/MS–MS) analysis. Antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis; 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Antibacterial pair with antibiofilm properties against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus were also determined, as well as cytotoxic activity on the MCF7 cell line model. The LC/MS–MS analysis of coffee extracts revealed high levels of caffeine; thus, the caffeine standard is used in all subsequent assays. Notably, robusta coffee extract showed remarkable antioxidant activity and selective inhibition of the growth against gram-positive and negative bacteria, along with the best inhibition of P. aeruginosa biofilm. However, compared with the caffeine standard, robusta coffee extract had lower cytotoxic activity and different bacterial targets in antibacterial and antibiofilm properties. Our results indicate that Robusta coffee extract is potentially a functional food due to its high alkaloid, phenolic, and flavonoid content and antioxidant activity, besides being used for natural compounds against bacterial infections.
Co-Authors Alfian Zurfi Analekta Tiara Perdana Anja Asmarany Apriza Yuswan Azisa Nabila Putri Bayo Alhusaeri Siregar Dewi Chusniasih Dwi Retnowati Erma Suryanti Fina Khaerunissa Frima I. Oktaviani Ika Agus Rini Ika Agus RINI Ika Agus Rini Indah D. Dewijanti Indri Yati Irmanida Batubara Leksikowati, Sovia Santi Mahyarudin, Mahyarudin Muhammad Eka Prastya Nadia Rahmah Novita Ariani Oktaviani, Indah Qurrota Ayun Revi Agustin Revi Agustin Riana, Elisa Nurma Salsabila Sugiarto Tarra Ivanka Tjandrawati Mozef Vera Permatasari Wulandari Putri Antika Y. Aryanti Yanti Ariyanti Yuni Lisafitri