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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan Biologi Undiksha Jurnal Sumberdaya Hayati BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Jurnal Biosense Quagga: Jurnal Pendidikan dan Biologi Maximus: Journal of Biological and Life Sciences TeknoKreatif: Jurnal Pengabdian kepada Masyarakat
Riana, Elisa Nurma
Institut Teknologi Sumatera
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Other

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Kandungan Logam Berat dan Vitamin E pada Testis Tikus Wistar setelah Dipaparkan Air Lindi Elisa Nurma Riana; Ayda T. Yusuf
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 4 No 1 (2021): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (542.961 KB) | DOI: 10.31539/bioedusains.v4i1.2239

Abstract

This study aims to determine the heavy metal content and vitamin E concentration of male Wistar rat testes exposed to Sarimukti landfill leachate. The research design used was a randomized block design. Rats were divided into four treatments, namely: aquabidestylates, 63% leachate, CdCl2, H2O2. The test substance was administered intraperitoneally for 14 days. Heavy metal concentrations were measured using the Atomic Absorption Spectrometry (AAS) method, while Vitamin E concentrations were measured using HPLC. The results showed that the concentration of heavy metal Zn in testicular tissue exposed to 63% leachate was 0.6 ppm, aquabidestylates was 0.5 ppm, while the other six metals had a concentration of 0 ppm. The measurement results of Vitamin E in the 63% leachate treatment were meager, namely, 1.06 ppm when compared to the aquabidestyl treatment of 7.92 ppm, CdCl2 and H2O2 were 0.53 ppm and 0.85 ppm, respectively. In conclusion, Sarimukti landfill leachate can cause an increase in ROS, which is indicated by a biomarker of decreased Vitamin E concentration in the testes, causing testicular damage. Keywords: Leachate, Heavy Metals, Wistar Rat Testicles, Vitamin E
Chemical Composition, Antioxidant, Antibacterial, Antibiofilm, and Cytotoxic Activities of Robusta Coffee Extract (Coffea canephora) Erma Suryanti; Dwi Retnowati; Muhammad Eka Prastya; Novita Ariani; Indri Yati; Vera Permatasari; Tjandrawati Mozef; Indah D. Dewijanti; Apriza Yuswan; Muhammad Asril; Elisa Nurma Riana; Irmanida Batubara
HAYATI Journal of Biosciences Vol. 30 No. 4 (2023): July 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.4.632-642

Abstract

Coffee extracts are a rich source of potential compounds with numerous biological activities. This study aimed to investigate the potential of chemical compounds derived from robusta coffee extract (Coffea canephora) and its dominant compound, caffeine, to scavenge free radicals, inhibit bacterial growth and biofilm formation, and cytotoxic properties in human breast adenocarcinoma cell line. Chemical constituents of coffee extract were analysed quantitively for total phenolic, flavonoid, and alkaloid contents, along with Liquid Chromatography Quadrupole-Mass Spectrometry (LC/MS–MS) analysis. Antioxidant activity was measured by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis; 3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Antibacterial pair with antibiofilm properties against Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Staphylococcus aureus were also determined, as well as cytotoxic activity on the MCF7 cell line model. The LC/MS–MS analysis of coffee extracts revealed high levels of caffeine; thus, the caffeine standard is used in all subsequent assays. Notably, robusta coffee extract showed remarkable antioxidant activity and selective inhibition of the growth against gram-positive and negative bacteria, along with the best inhibition of P. aeruginosa biofilm. However, compared with the caffeine standard, robusta coffee extract had lower cytotoxic activity and different bacterial targets in antibacterial and antibiofilm properties. Our results indicate that Robusta coffee extract is potentially a functional food due to its high alkaloid, phenolic, and flavonoid content and antioxidant activity, besides being used for natural compounds against bacterial infections.
Deteksi Konsentrasi Fe, Cu, Zn dan Pb Air Sungai dan Ikan yang Tercemar Air Lindi di Bandar Lampung Dengan Menggunakan SSA: Detections of Fe, Cu, Zn and Pb Concentration in River Water and Fish Polluted by Leachate in Bandar Lampung Using AAS Andriyana, Ahmad Dodi; Mulyana, Jeane Siswitasari; Khairani, Iffa Afiqa; Riana, Elisa Nurma
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.97-101

Abstract

Leachate is a pollutant produced by landfills which can pollute the aquatic environment. This will have an impact on fish that live in waters contaminated with leachate and if consumed by the public it will harm health. The toxic effects of leachate are caused by the content of toxic chemicals, one of which is heavy metals. The aim of this research was to determine the concentrations of Fe, Cu, Zn and Pb in Bakung landfill leachate, Sukamaju river water and fish meat. Bakung landfill leachate samples were taken from two outlet points. The fish sampling location was carried out in the Sukamaju River. The physical factors of water measured are temperature, pH, salinity, turbidity, DO, COD and BOD. Fe, Cu, Zn and Pb concentrations were measured using the Atomic Absorption Spectrophotometry (AAS) method. The results show that the physicochemical parameters in leachate and river water exceed the threshold. The contents of Zn, Cu, Fe and Pb in the leachate at both outlet points exceeded the threshold value with values of 2.48 mg/L and 2.80 mg/L; 16.55 mg/L and 17 mg/L; 0.078 mg/L and 0.113 mg/L; 3.26 mg/L and 3.01 mg/L. The concentration of heavy metals Pb and Fe in river water is 0.083-0.162 mg/L and 2.68-2.97 mg/L which are above the quality standard value, while the heavy metal Fe content in fish meat exceeds the specified threshold value, namely 0.557 mg/100 g and 0.564 mg/100 g.
Sosialisasi Potensi Etnobotani dan Etnozoologi Desa Pahawang Maretta, Gres; Nurhayu, Winati; Novriadi, Novriadi; Yanti Ariyanti, Yanti Ariyanti; Wahyuni, Kurnia; Riana, Elisa Nurma; Khairani, Iffa Afiqa; Darmawan, Andy; Leksikowati, Sovia Santi; Kesuma, Andri Jaya
Abdimas Galuh Vol 7, No 1 (2025): Maret 2025
Publisher : Universitas Galuh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25157/ag.v7i1.17818

Abstract

Etnobiologi adalah evaluasi ilmiah terhadap pengetahuan penduduk tentang biologi, meliputi pengetahuan tentang tumbuhan (etnobotani), hewan (etnozoologi) dan lingkungannya (etnoekologi). Kajian etnobiologi dapat menghubungkan biologi dan etnis yang ada pada suatu tradisi adat. Melalui kegiatan Pengabdian Kepada Masyarakat, mendorong masyarakat untuk peduli terhadap pelestarian sumber daya alam yang ada di Desa Pahawang. Hasil akhir dari pelaksanaan kegiatan adalah pengetahuan masyarakat tentang pemanfaatan etnobotani dan etnozoologi yang ada di desa Pahawang. Kami melakukan penilaian terhadap pengetahuan dan ketertarikan masyarakat mengenai pemanfaatan etnobotani dan etnozoologi yang ada dan diterapkan di desa Pahawang. Pengetahuan masyarakat mengenai potensi dan pemanfaatan etnobotani dan etnozoologi di desa Pahawang meningkat setelah pelatihan jika dibandingkan dengan sebelum pelatihan. Hal ini dapat dilihat dari ketercapaian tujuan dari kegiatan dari hasil pengolahan data kuesioner. Rerata ketercapaian tujuan dapat dikatakan baik (75,6%) berdasarkan dari hasil pre-test dan post-test, dimana terdapat peningkatan pengetahuan dari ibu-ibu peserta kegiatan sebesar dari awal hingga akhir kegiatan. Kemampuan melakukan budidaya tanaman pangan, dan pengetahuan tentang pengobatan alami merupakan potensi besar untuk dikembangkan demi meningkatkan ketahanan pangan dan kesehatan yang lebih baik bagi masyarakat Desa Pahawang ke depan.
IMMUNOSTIMULATORY EFFECT OF VITAMIN C ON MICE INDUCED BY STAPHYLOCOCCUS AUREUS Khairani, Iffa Afiqa; Riana, Elisa Nurma; Maretta, Gres; Azizah, Najla Nur; Putri, Isnaini Anggraini; Maruli, Berkat Afni; Mulyadi, Salsa Pratiwi; Antonius, Antonius
MAXIMUS: Journal of Biological and Life Sciences Vol. 1 No. 2 (2023)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/maximus.v1i2.1636

Abstract

Several compounds that can boost immune activity (immunostimulatory) include vitamin E, curcumin, flavonoids, and vitamin C. In this study, ascorbic acid or vitamin C, was used as one method to enhance the immune system. This study aims to determine the immunostimulatory effect of vitamin C on the phagocytosis activity of macrophages in mice induced by Staphylococcus aureus. The mice were divided into two groups: the control group, which received 0.3 ml of distilled water orally, and the treatment group, which received 0.036 mg/g BW of vitamin C orally for 14 days. On the 15th day, each mouse was intraperitoneally induced with 0.5 mL of Staphylococcus aureus bacterial suspension and left for two hours. The phagocytic activity value represents the percentage of active macrophage cells among all macrophages observed. The result showed that the value of % phagocytic activity in the control group was 21.43% ± 7.92, whereas the % phagocytic activity in the treatment group was 48.31% ± 22.27. The group of mice given vitamin C 0.036 mg/g BW orally showed increased phagocytic activity compared to the control group mice. This shows that vitamin C has potential as an immunostimulator.
Quantitative Analysis of Phytochemical Compounds and Antihyperglycemic Potential of Robusta Coffee from West Lampung Khairani, Iffa Afiqa; Mulyana, Jeane Siswitasari; Olivia, Rahel Noviantika; Riana, Elisa Nurma; Anisa, Hida Arliani Nur
Jurnal Sumberdaya Hayati Vol. 10 No. 1 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.1.1-6

Abstract

West Lampung Regency in Lampung Province produces high quality robusta coffee with a distinct, strong bitter flavour. Bitter flavour indicates the amount of bioactive substances produced by plants called phytochemicals. The aim of this study is to analyse the phytochemical compounds and antihyperglycemic potential of robusta coffee beans from West Lampung, Indonesia. Quantitative phytochemical analysis was carried out using the thin layer chromatography method and spectrophotometry. Animal experimental design using robusta coffee on hyperglycemia conditions in mice which was induced by alloxan 170 mg/kgBW subcutaneously and given by 1 ml of brewed robusta coffee for 10 days. The robusta coffee from West Lampung contained total caffeine 4,014.87 µg/g, total flavonoid content (TFC) 93.6 mg quercetin equivalent per gram of sample, with total alkaloid content (TAC) 0.848 mg of quinine equivalent per gram of sample, total tannin content (TTC) 182.3 mg of tannic acid equivalent per gram of sample, and total saponin content (TSC) 24.2 mg of quillaja bark equivalent per gram of sample. The administration of 1 ml/mice/day of robusta coffee brewed for 10 days did not show a decrease in blood sugar level in hyperglycemic mice, due to the short duration of the study, so the role of coffee in decreasing hyperglycemia conditions has not been optimally observed.
Antioxidant Activity and Immunomodulatory Effect of Stachytarpheta sp. Leaf Extract on Phagocytic Activity of Mice Peritoneal Macrophages in Alloxan Induction Iffa Afiqa Khairani; Wahyuni Dian Lestari; Elisa Nurma Riana; Jeane Siswitasari Mulyana; Hida Arliani Nur Anisa; Rizka Fatriani; Silvia Andriani
Quagga: Jurnal Pendidikan dan Biologi Vol 17 No 2 (2025): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v17i2.404

Abstract

Hyperglycemia is a condition characterized by elevated blood glucose levels, which is a hallmark of diabetes mellitus. Prolonged hyperglycemia not only disrupts glucose homeostasis but also impairs the immune response. The condition of hyperglycemia in the body can increase free radicals and oxidative stress, thus can impair various macrophage functions, including their ability to engulf and eliminate pathogens. The bioactive constituents in Stachytarpheta sp. may contribute to reducing oxidative damage and enhancing immune system function. This study aims to evaluate both the antioxidant capacity and the immunomodulatory effects of Stachytarpheta sp. leaf extract on the phagocytic activity of peritoneal macrophages in alloxan-induced diabetic mice. Alloxan is widely used in research to induce hyperglycemia (diabetic conditions) in experimental animals. The antioxidant activity of the extract was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay, while phagocytic activity of peritoneal macrophages was performed in vivo using male mice. Stachytarpheta sp. leaf extract from Beteng Sari Village, East Lampung has very strong antioxidant activity against DPPH with IC50 value 14,85 ± 0,30 μg/mL. This extract at doses of 200, 300, and 400 mg/kgBW can be an immunomodulator that maintains the phagocytic activity of macrophage cells in alloxan-induced mice.
Efek Pemberian Ekstrak Kulit Kabau (Archidendron jiringoides komariah) Terhadap Konsentrasi Spermatozoa Mencit Jantan yang Diinduksi Timbal (Pb) Elisa Nurma Riana; Yanti Ariyanti
Jurnal Pendidikan Biologi Undiksha Vol. 11 No. 1 (2024)
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/jjpb.v11i1.79441

Abstract

Infertility is currently an important problem in the world, including in Indonesia. Infertility can occur in men and women. Inmen, factors causing infertility can come from external sources. One environmental factor that can cause infertility is exposureto heavy metals from the environment. One way to prevent and treat infertility includes consuming natural antioxidants derivedfrom plants. One plant that has antioxidant compounds and has antioxidant activity is the kabau plant. This research wasconducted to test the effect of kabau seed pod extract (Archidendron jiringoides Komariah) on male mice exposed to Pb metalto determine its effect on the concentration of spermatozoa in male mice. The methods used in this research include:preparation of test animals, making simplicia and kabau seed pod extract, exposing the extract to test animals, isolatingorgans, and making spermatozoa suspensions. Giving kabau seed pod extract to mice that had previously been exposed tolead (Pb) increased the concentration of mice spermatozoa with the most effective dose of kabau bark extract, namely 190mg/kg BW. However, statistical analysis shows that the increase is not significant.
EFEK EKSTRAK KULIT BUAH KABAU (Archidendron bubalinum (Jack) I.C Nielsen) TERHADAP AKTIVITAS MAKROFAG MENCIT JANTAN SETELAH DIPAPARKAN CdCl2 Elisa Nurma Riana; Iffa Afiqa Khairani; Hida Arliani Nur Anisa; Rizka Fatriani; Silvia Andriani
JURNAL BIOSENSE Vol 9 No 2 (2026): Edisi April 2026
Publisher : Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i2.7817

Abstract

Logam kadmium (Cd) yang berasal dari kegiatan industri seperti produksi baterai, pelapisan logam, penggunaan pupuk fosfat, pembakaran bahan bakar fosil, dan pembuatan plastik dapat meningkatkan produksi reactive oxygen species (ROS). Produksi ROS berlebih dalam tubuh akan menyebabkan penyakit dan mempengaruhi sistem imun tubuh akibat stres oksidatif. Stres oksidatif merupakan suatu kondisi ketidakseimbangan antara radikal bebas dan antioksidan. Untuk melindungi tubuh dari stres oksidatif diperlukan suatu senyawa antioksidan, seperti yang terkandung dalam kulit buah kabau (Archidendron bubalinum). Penelitian ini bertujuan mengetahui mengetahui adanya peningkatan aktivitas fagositosis makrofag pada mencit Jantan yang dipaparkan CdCl2. Penelitian ini bersifat eksperimental menggunakan rancangan acak lengkap (RAL). Mencit yang digunakan 24 ekor dengan 6 kelompok yang terdiri dari 3 kelompok kontrol dan 3 kelompok perlakuan. Hasil pengujian menunjukkan bahwa pemberian ekstrak kulit buah kabau dosis 95 mg/kgBB, 190 mg/kgBB dan 380 mg/kgBB pada uji aktivitas fagositosis makrofag menunjukkan hasil yang signifikan sebesar p = 0,005. Hal ini berarti bahwa ekstrak kulit buah kabau dapat meningkatkan sistem imun mencit dengan perlakuan P2 memiliki aktivitas fagositosis tertinggi yaitu 55,91%.
Aksi Bina Kesehatan Reproduksi dengan Potensi Tanaman Obat Keluarga di Kelurahan Way Dadi, Bandar Lampung Fajar Islam Sitanggang; Elisa Nurma Riana; Hendry Wijayanti; Erma Suryanti
TeknoKreatif: Jurnal Pengabdian kepada Masyarakat Vol 3 No 1 (2023): TEKNOKREATIF : Jurnal Pengabdian kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M), Institut Teknologi Sumatera, Lampung, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/teknokreatif.v3i1.739

Abstract

Reproductive health is one of the knowledge that is still taboo in society. In fact, it is very important for reproductive development. In adolescents, physical, mental, and social changes must be balanced with good and correct knowledge. Thus, a healthy and quality generation can be created. Way Dadi Village, is one of the villages located in Sukarame District, Bandar Lampung City with a fairly high number of teenagers and adults. This Community Service (PKM) aims to provide knowledge to the public about the importance of reproductive health in maintaining the quality of generations and provide knowledge about the importance of knowing the potential of Medicinal Plants (TOGA) which can be used as prevention of reproductive diseases. This PKM is carried out with a face-to-face method in accordance with health protocols. The PKM method is carried out by evaluating the pre-instruction and post-instruction research questionnaires. Filling in is based on the weighting of understanding starting from not, enough, moderate, good, and very good. Thus, the final post-exam results are obtained with a very good average understanding of up to 86%.
Co-Authors Andri Jaya Kesuma Andriyana, Ahmad Dodi ANDY DARMAWAN Anisa Mumtaz Zahra Anisa, Hida Arliani Nur Antonius Antonius Antonius Antonius Apriza Yuswan Ayda T. Yusuf Azizah, Najla Nur Dwi Retnowati Erma Suryanti Erma Suryanti Fajar Islam Sitanggang Gebriel Nurcahya Gres Maretta, Gres Hendry Wijayanti Hida Arliani Nur Anisa Hida Arliani Nur Anisa Iffa Afiqa Khairani Indah D. Dewijanti Indri Yati Irmanida Batubara Jeane Siswitasari Mulyana Kurnia Wahyuni Leksikowati, Sovia Santi Maruli, Berkat Afni Muhammad Asril Muhammad Eka Prastya Mulyadi, Salsa Pratiwi Mulyana, Jeane Siswitasari Novita Ariani Novriadi, Novriadi Nurhayu, Winati Olivia, Rahel Noviantika Putri, Isnaini Anggraini Rizka Fatriani Sari, Dian Anggria Silvia Andriani Tjandrawati Mozef Vera Permatasari Wahyuni Dian Lestari Winati Nurhayu Yanti Ariyanti Yanti Ariyanti, Yanti Ariyanti