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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Biology, Medicine, & Natural Product Chemistry Molecular and Cellular Biomedical Sciences (MCBS) Makara Journal of Science Jurnal Kefarmasian Indonesia
Rifa'i, Muhaimin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Journal : The Journal of Experimental Life Sciences (JELS)

 1 
The Comparison Effect of DOCA and L-NAME on T Cells Activation in the Preeclampsia Mice Model Khodijah, Riska Amalia; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rahayu, Sri; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.03

Abstract

Preeclampsia is a systemic disorder of pregnancy characterized by the maternal immune system's failure to properly adapt to fetal antigens, resulting in chronic inflammatory responses. This research aimed to examine the effects of DOCA (deoxycorticosterone acetate) and L-NAME (Nω-nitro-L-arginine methyl ester) in inducing preeclampsia in pregnant BALB/c mice, focusing on lymphocyte activation. Twenty-four BALB/c mice were randomly divided into four groups: NP (non-pregnant mice), P (healthy pregnant mice), D (pregnant mice received DOCA), and L (pregnant mice received L-NAME). DOCA was dissolved in corn oil and administered subcutaneously at 12.5 mg.kg-1 BW before pregnancy and 6.5 mg.kg-1 BW weekly during pregnancy. L-NAME was administered daily starting from day 5 of pregnancy. Mice were dissected on day 16 of pregnancy, and the spleen was isolated to analyze the relative number of CD4+CD62L+ and CD8+CD62L+ T cells. The results showed that DOCA and LNAME exhibited fewer CD4+CD62L+ and CD8+CD62L+ T cells than healthy pregnant mice. The administration of DOCA at 12.5 mg.kg-1 BW (before mating) and 6.5 mg.kg-1 (day 7 of pregnancy) was more effective in triggering the activation of CD4+CD62L+ and CD8+CD62L+ T cells than the others indicated by the lowest number of CD4+CD62L+ and CD8+CD62L+ T cells. However, the conclusion of which one is more effective in inducing preeclampsia has not been reached. This is due to the many parameters that must be observed related to preeclampsia. Further studies are needed, especially examining proinflammatory cytokine production in T cells after induction with both inducers. Keywords: DOCA, L-NAME, Lymphocyte, Mice, Preeclampsia.
Evaluation of TNF-α and IFN-γ Levels in Macrophage of Preeclampsia Mice Model Induced by DOCA and L-NAME Susanti, Winda Karina; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rahayu, Sri; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 3 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.03.05

Abstract

Preeclampsia is a pregnancy disorder characterized by high blood pressure, proteinuria, and chronic inflammation that potentially leads to multiorgan failure. This study investigated differences in the relative number of pro-inflammatory cytokines (IFN-γ & TNF-α) in preeclamptic mice induced by DOCA and L-NAME. Eighteen pregnant BALB/c mice (n=6): pregnant mice without any treatment (P), pregnant mice treated with Deoxycorticosterone acetate/DOCA (D), and pregnant mice treated with N-ω-L-arginine Methyl Ester/L-NAME (L) and healthy nonpregnant mice as control (NP). DOCA at 12.5 mg.kg-1 BW was injected subcutaneously before mating and 6.5 mg. kg-1 BW after mating every week for 16 days. L-NAME was administered orally at 75 mg.kg-1 BW daily after five days of gestation. Mice were dissected in the second trimester (day 16), and then the spleen was isolated to analyze the relative number of CD11b+IFN-γ+ and CD11b+TNF-α+ using flow cytometry analysis. The results showed that DOCA and L-NAME induction did not significantly induce the production of TNF-α levels in the macrophage of pregnant mice. However, both DOCA and L-NAME significantly induce the production of IFN-γ levels in the macrophage of pregnant mice. Further evaluation of other markers related to preeclampsia should be done to better understand the roles of DOCA and L-NAME in inducing preeclampsia. Keywords: DOCA, L-NAME, mice, preeclampsia, pregnant.
Phytochemical Profiles, DPPH Radical Inhibition Effectiveness, and Cytotoxic Potential of Polyherbal Plants in T47D Cell Line Wahyuningsih, Nadia; Rahayu, Sri; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.02

Abstract

Breast cancer subtypes include luminal A, luminal B, HER2, triple-negative, and normal-like. Antioxidants, ROS, and cancer have a close relationship. Cancer cells exhibit an elevated ROS level, which can be used for selective cell-based therapeutic targeting. Therefore, developing potential therapeutic potential therapeutic options is crucial. Herbal medicine has gained much interest in treating cancer. Indian jujube (Ziziphus mauritiana), cumin (Nigella sativa), turmeric (Curcuma longa), soursop (Annona muricata), and meniran (Phylanthus niruri) potentially have a role as anticancer agents and were used as alternative medicine. This study aimed to determine the phytochemicals total content, DPPH radical inhibition, and cytotoxicity of the polyherbal plants to the T47D cell line. The antioxidant analysis was conducted using DPPH assay, and the phytochemicals analysis was determined using total flavonoid (TFC), phenolic (TPC), and alkaloid (TAC) content. The cell viability assessment was performed using the WST-1 method. The antioxidant analysis revealed that the IC50 value of polyherbal medicine is 213.65 µg.ml-1. The phytochemical analysis showed that the highest content is the flavonoid compared to others, with a value of 138.986 mgQE.mg-1. Other than that, the cell viability assessment showed that the IC50 of the herbal medicine is 480.8 µg.mL-1. These findings indicated that combining turmeric, cumin, meniran, Indian jujube, and soursop has moderate cytotoxicity against the T47D cell line since it can reduce T47D cell line viability at high doses. Keywords: antioxidant, breast cancer, herbal medicine, phytochemicals, T47D cell line.
Elephantopus scaber L. Ethanolic Leaves Extract Modulates IL-2 Production and T-Lymphocyte Activation in Pulmonary Fibrosis Mice Model Izzah, Fathiyah Nurul; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Rifa'i, Muhaimin; Djati, Muhammad Sasmito
The Journal of Experimental Life Science Vol. 14 No. 1 (2024)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2024.014.01.01

Abstract

Pulmonary fibrosis is a chronic disease characterized by progressive connective tissue deposition that replaces healthy lung tissue. This study aimed to investigate the effect of Elephantopus scaber L. Ethanol Extract (ESEE) treatment on the relative number of IL-2 cytokine and lymphocyte activation in bleomycin (BLM)-induced pulmonary fibrosis mice model. Fifty-six male BALB/c mice were divided into seven treatment groups: N (normal); V or vehicle (corn oil); PF or Pulmonary Fibrosis (BLM 2 mg.kg-1); Dex (Dexamethasone 3 mg.kg-1 + BLM); D1-D3 (ESEE at doses of 0.0504, 0.1008, and 0.2016 mg.kg-1 BW + BLM). ESEE, dexamethasone, and corn oil were administered orally, followed by intraperitoneal bleomycin injection daily for 14 days. Mice were dissected on days 7 and 14, and spleens were isolated to analyze cell populations expressing CD4+IL-2+, CD8+IL-2+, CD4+CD62L+, and CD8+CD62L+. The results showed that bleomycin injection could increase the relative number of IL-2 and decrease the relative number of naive T cells compared to normal mice. ESEE treatment significantly reduced the relative number of IL-2, thus decreasing naive T cell activation after one week of bleomycin injection compared to the mice model. In contrast, the increased IL-2 production led to the increasing naive T cell activation after two weeks of bleomycin injection. Therefore, ESEE treatment has the potential to maintain homeostasis through modulation of IL-2 production and T-lymphocyte activation in the pulmonary fibrosis mice model. Keywords: Elephantopus scaber, IL-2, lymphocytes, mice, pulmonary fibrosis.
Co-Authors Al Faizah, Belinda Nabiila Al-Faizah, Belinda Nabiila Ardiansyah, Esha Aris Soewondo Askandar Tjokroprawiro Atho'illah, Mochammad Fitri Atho’illah, Mochammad Fitri Dinia Rizqi Dwijayanti Djanggan Sargowo Djati, Mohammad Sasmito Estri Laras Arumingtyas Fadlilah, Dawama Nur Farida Rachmawati, Farida Fatinah, Arik Arubil Izati, Rahmi Izzah, Fathiyah Nurul Kavitarna, Septhyanti Aprilia Khodijah, Riska Amalia Moch Sasmito Djati Nashi Widodo Sa'adah, Nur Alfi Maghfirotus Sakti, Muhammad Wisam Wira Sakti, Sefihara Paramitha Sari, Fikriya Novita Shinta Oktya Wardhani, Shinta Oktya Shofiyah, Aya Siti Nur Arifah, Siti Nur SRI RAHAYU Sri Widyarti Susanti, Winda Karina Totowiputro, Dicky Kurniawan Tsuboi, Hideo Wahyuningsih, Nadia Widekdo, Dwi purno Wisnu Barlianto YOGA DWI JATMIKO Yuyun Ika Christina