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All Journal Science and Technology Indonesia Jurnal Kartika Kimia Stannum : Jurnal Sains dan Terapan Kimia Jurnal Abdi Insani Mestaka: Jurnal Pengabdian Kepada Masyarakat
Ambarwati, Yuli
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Activity of Mangrove-Derived Fusarium equiseti 20CB07RF Extract Against Clinical, Antibacterial-Resistant Pseudomonas aeruginosa Bahri, Syaiful; Setiawan, Wawan Abdullah; Setiawan, Fendi; Lutfiah, Rosyidatul; Juliasih, Ni Luh Gede Ratna; Ambarwati, Yuli; Ahmadi, Peni; Arai, Masayoshi; Hendri, John; Hadi, Sutopo; Setiawan, Andi
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.594-604

Abstract

Endophytic fungi originating from mangroves are potential sources of secondary metabolites with varying bioactivities. This research explores the bioactive metabolites produced by endophytes derived from mangrove plants. Endophytic fungi were collected from various parts of several mangrove plants (roots, stems, and leaves, as well as the surrounding mud). A total of 17 endophytics fungi were obtained. The isolates were derived from the leaves (1 isolate), stems (8 isolates), roots (5 isolates), and surrounding mud (3 isolates). A single fungal colony was cultured using solid-state fermentation for 14 days. The fermented fungal biomass was extracted using ethyl acetate (EtOAc) and evaluated for its antibacterial activity against clinical pathogenic bacteria. In the preliminary screening, the EtOAc extract of the CB07RF1 isolate exhibited notable growth-inhibitory effects against Pseudomonas aeruginosa. The isolate was verified by molecular identification using a study of the rDNA internal transcribed spacer (ITS) sequence, revealed that isolate CB07RF1 was very similar to Fusarium equiseti (99% similarity). Isolate 20CB07RF1, obtained by solid-state fermentation using a rice medium indicated as peptide compound group, and featured active components that exhibited potent growth inhibitory activity against Pseudomonas aeruginosa at a concentration of 12.5 mg/mL. This study demonstrates, for the first time, that Fusarium equiseti extracts grown in a rice medium contain antimicrobial compounds that can inhibit the growth of P. aeruginosa, an important clinical pathogen known for its antibacterial resistance. These findings accent mangrove endophytic fungi as important sources of bioactive compounds and will advance related research in the fields of biotechnology, pharmacology, and life sciences.
Uji Bioinsektisida Ekstrak Buah Bintaro dan Umbi Gadung Terhadap Hama Walang Sangit (Leptocorisa acuta T.) Bahri, Syaiful; Ilim, Ilim; Qudus, Hardoko Insan; Ambarwati, Yuli; Wulandari, Ika Rizki
Jurnal Kartika Kimia Vol 6 No 1 (2023): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v6i1.189

Abstract

Insecticides are a type of pesticide that is commonly used by farmers to eradicate insect pests, one of which is the stink bug. Excessive use results in resistance and contamination of soil, water, and air. Another alternative is bioinsecticides which are safe and do not cause resistance. In this study, the samples used were gadung and bintaro root extracts. The method used is maceration, after the maceration process, the filtrate will be tested on the target animal, looking for the best insecticide mortality and screening for phytochemicals. Lastly, the best bioinsecticide solutions were identified using FT-IR and UV-Vis spectrophotometers. The best bioinsecticide solutions with 60% and 40% mortality were bioinsecticides with a concentration of 70% gadung and 30% gadung 20% bintaro. Phytochemical screening tests were carried out on both of them, the results were positive for terpenoids and alkaloids. Identification using the FTIR spectrophotometer showed that the bioinsecticide solution contained secondary metabolites with O-H groups appearing at absorption wave number 3260 cm-1, C=O groups appearing at wave number 1637cm-1,, and C-H groups appearing at wave number 1359 cm-1. The results of the UV-Vis spectrum showed that the bioinsecticide solution had the C=C functional group as indicated by the appearance of peaks at a wavelength of 210 nm, 217 nm, and 223 nm.
Pembuatan Pompa Air Tanpa Mesin Sebagai Sarana Irigasi Di Dataran Tinggi Pekon Bumi Ratu Hendri, John; Mulyono, Mulyono; Khotimah, Siti Nurul; Purwadi, Ofik Taupik; Bahri, Syaiful; Laila, Aspita; Ambarwati, Yuli
MESTAKA: Jurnal Pengabdian Kepada Masyarakat Vol. 3 No. 6 (2024): Desember 2024
Publisher : Pakis Journal Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58184/mestaka.v3i6.532

Abstract

Bumi Ratu Village is located on the edge of the Way Sekampung dam, with the position of the dam lower than the village land. This causes the agricultural land and rice fields of Bumi Ratu Village to have difficulty getting water, because the irrigation flow cannot reach a higher position. This problem is an important concern because it greatly affects the planting season and harvest from agriculture. The purpose of this service is to overcome the problem of irrigation of land that has a geographical location above the river level through appropriate technology in the form of a water pump without a machine (hydram pump). The method of this activity consists of several steps, namely 1) Determination of location points that have water sources, 2) Making hydram pump equipment, 3) Socialization, 4) Hydram Pump Trial and 4) Evaluation. This community service activity is expected to be an effective solution in overcoming irrigation problems on their agricultural land. The results of this community service activity show that hydram pumps are an appropriate solution to the irrigation problems that occur in Bumi Ratu Pagelaran Village. Through the use of this hydram pump, farmers and landowners are expected to optimize land irrigation around the highland area so that crop yields can be as expected.
Utilization of Organic Waste into Immunity Protein Products and Its Application in Broiler Chicken Growth Bahri, Syaiful; Berghuis, Nila Tanyela; Ambarwati, Yuli; Sari, Revita; Nurfaradina, Zaskia Ayu
Stannum : Jurnal Sains dan Terapan Kimia Vol 7 No 1 (2025): April 2025
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v7i1.5678

Abstract

This research is related to utilizing organic liquid waste to be processed into immune protein. The processing stages include a mixture of organic liquid waste in the form of garbage leachate, Zingiberaceae, Crude Palm Oil, beef animal fat, and old coconut water. Mixing was carried out by making two immune protein products that differed in composition and function including protim 1 and protim 2 in knowing the success of the product from the observation of the weight of broilers at harvest. Giving protim 1 in drinking produced broiler weights up to 18 days of the age of 707, 697, and 634 grams. The weight of broilers obtained from the provision of protim 2 in drinking until the end of harvest was 2732, 2670, and 2458 grams. The use of both protims was successfully tested by comparing broilers that did not use protim until harvest, resulting in weights of 1749, 2238, and 2338 grams. FCR and IP values for three broilers consuming both protims resulted in values of 0.66 and 11.34, respectively, with physical characteristics that were slightly hairy compared to broilers that did not consume protim.
Sosialisasi dan Pembuatan Biostimulan Cair Berbasis Limbah Organik untuk Mendukung Pertanian Berkelanjutan Bahri, Syaiful; Ambarwati, Yuli; Widiarto, Sonny; Annisa, Devi Nur; Nurhasanah, Nurhasanah; Ayumi, Eksa
Mestaka: Jurnal Pengabdian Kepada Masyarakat Vol. 4 No. 2 (2025): April 2025
Publisher : Pakis Journal Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58184/mestaka.v4i2.619

Abstract

The improper management of household organic waste often leads to environmental pollution and health issues. This community service program aims to educate the residents of Rejo Basuki village on processing organic waste into liquid organic fertilizer (POC) using the Biostimulant Liquid (BL) method. This method was chosen for its ability to accelerate the decomposition of organic materials without requiring a lengthy fermentation process, offering a practical and efficient waste management solution. The program was conducted through lectures and hands-on training on POC and organic pesticide production, led by lecturers from the Department of Chemistry, FMIPA, University of Lampung. The results showed that BL-based POC significantly improved soil structure, increased nutrient availability, and enhanced plant resistance to pests and diseases. Additionally, participants gained valuable knowledge on sustainable and economically beneficial organic waste management practices. This innovation is expected to be widely adopted to support sustainable and environmentally friendly agriculture.
Pembuatan Pupuk Organik Cair Pembenah Tanah dari Lindi dan Aplikasinya Pada Tanaman Cabai, Tomat, Terong Bahri, Syaiful; Ambarwati, Yuli; Rinawati, Rinawati; Widiarto, Sony; Hardiyanto, Candra
Jurnal Kartika Kimia Vol 8 No 1 (2025): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v8i1.232

Abstract

Liquid organic fertilizer is a solution from the decay of organic materials whose basic ingredients come from animals or plants that have undergone fermentation. This research aims to process traditional market organic waste with the addition of biang which can produce a product in the form of liquid organic fertilizer derived from the combination of biang and leachate. In this research, soil improver liquid organic fertilizer samples were tested and according to a ministerial degree of Agriculture of the Republic of Indonesia No. 261 of 2019 concerning Organic Fertilizers, Biofertilizers and Soil Improvement. Applications were made on topsoil, subsoil 1, and subsoil 2 containing chili, tomato, and eggplant plants and plant height observations were made for 30 days. Laboratory tests of liquid organic fertilizer for soil improvement, the results obtained only the parameters of heavy metals, pH, C-organic and micronutrients (Fe) that have met the quality standards. The results of plant height observations obtained a significant increase in plant growth and the application of liquid organic fertilizer soil improver can make subsoil soil compete with topsoil soil. Further research needs to be done by utilizing other alternative materials to increase the levels of macro and micronutrients and so that the results obtained can meet the quality standards used.
MEMBANGUN DESA MANDIRI ENERGI MELALUI PENGOLAHAN SAMPAH PLASTIK MENJADI BBM ALTERNATIF DI DESA HANURA KECAMATAN TELUK PANDAN PESAWARAN Bahri, Syaiful; Ambarwati, Yuli; Waluyo, Sri; Oktarina, Rasmi Zakiah; Notiragayu, Notiragayu
Jurnal Abdi Insani Vol 11 No 4 (2024): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v11i4.2025

Abstract

The market in Hanura Village faces serious problems related to rubbish, especially because it is located close to the main beach tourist route which is busy during holidays. Increased activity in markets produces more waste, which is usually simply transferred to landfill, but this method is less effective. Plastic waste is the second biggest problem after organic waste and is difficult to decompose naturally, so other solutions are needed to reduce waste accumulation. To tackle this issue, the Unila community service team proposes a more efficient plastic waste management system. Drawing from Bahri et al., (2019), their research demonstrates that plastic waste can be converted into alternative fuel through a pyrolysis process, utilizing a device known as a pyrolizers. With an abundant supply of plastic waste and Hanura Village’s strategic location, the development and application of this process are being implemented through a community service program based on research dissemination. The result of this activity is a plastic waste processing tool that converts waste into alternative fuel in the form of a pyrolyzer with three condensers. The fuel produced from the pyrolysis of plastic waste is characterized by its chromatogram profile using Gas Chromatography Mass Spectrometry (GCMS), and its calorific value is measured using a bomb calorimeter, while the octane number is determined using an Octane Number Tester.  The results of the community service activity in Hanura Village cognitively show that the residents of Hanura Village were highly enthusiastic about receiving the training, especially related to the processing of plastic waste into fuel, with an overall achievement of specific instructional objectives reaching 53%.
Co-Authors Ahmadi, Peni Andi Setiawan Annisa, Devi Nur Arai, Masayoshi Aspita Laila Ayumi, Eksa Berghuis, Nila Tanyela Hardiyanto, Candra Ilim, Ilim John Hendri Juliasih, Ni Luh Gede Ratna Lutfiah, Rosyidatul Mulyono Mulyono Notiragayu, Notiragayu Nurfaradina, Zaskia Ayu Nurhasanah Nurhasanah Oktarina, Rasmi Zakiah Purwadi, Ofik Taupik Qudus, Hardoko Insan Revita Sari Rinawati Rinawati Setiawan, Fendi Siti Nurul Khotimah Sri Waluyo Sutopo Hadi SYAIFUL BAHRI WAWAN ABDULLAH SETIAWAN, WAWAN ABDULLAH Widiarto, Sonny Widiarto, Sony Wulandari, Ika Rizki